TALENs Mediate Efficient and Heritable Mutation of Endogenous Genes in the Marine Annelid Platynereis dumerilii
Open accessAbstract
Platynereis dumerilii is a marine polychaete and an established model system for studies of evolution and development. Platynereis is also a re-emerging model for studying the molecular basis of circalunar reproductive timing: a biological phenomenon observed in many marine species. While gene expression studies have provided new insight into patterns of gene regulation, a lack of reverse genetic tools has so far limited the depth of functional analyses in this species. To address this need, we established customized transcriptional activator-like effector nucleases (TALENs) as a tool to engineer targeted modifications in Platynereis genes. By adapting a workflow of TALEN construction protocols and mutation screening approaches for use in Platynereis, we engineered frameshift mutations in three endogenous Platynereis genes. We confirmed that such mutations are heritable, demonstrating that TALENs can be used to generate homozygous knockout lines in P. dumerilii. This is the first use of TALENs for generating genetic knockout mutations in an annelid model. These tools not only open the door for detailed in vivo functional analyses, but also can facilitate further technical development, such as targeted genome editing.
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Platynereis is molecularly accessible, and various genetic toolkits allow functional studies.
“Moreover, this species is molecularly accessible, and various genetic toolkits allow functional studies (9–11).”
From Corazonin signaling integrates energy homeostasis and lunar phase to regulate aspects of growth and sexual maturation in <i>Platynereis</i> (2020) · cites this paper as [11]
The marine bristleworm Platynereis dumerilii is well suited to tackle the endocrine basis of this integration.
“The marine bristleworm Platynereis dumerilii is well suited to tackle the endocrine basis of this integration.”
From Corazonin signaling integrates energy homeostasis and lunar phase to regulate aspects of growth and sexual maturation in <i>Platynereis</i> (2020) · cites this paper as [11]
The number of spawning animals resulting from this regulation is lowest during the full moon phase.
“the number of spawning animals resulting from this regulation is lowest during the full moon phase (8, 11)”
From Corazonin signaling integrates energy homeostasis and lunar phase to regulate aspects of growth and sexual maturation in <i>Platynereis</i> (2020) · cites this paper as [11]
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BackgroundMethodČermák, T., Doyle, E., Christian, M. L., Wang, L., Zhang, Y., Schmidt, C. L., Baller, J. A., Somia, N. V., Bogdanove, A. J., & Voytas, D. F. (2011) · Nucleic Acids Research · doi:10.1093/nar/gkr218 · Cited by 2,034 · cited 6× · see citing works
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BackgroundMethodInfluentialTargeting DNA Double-Strand Breaks with TAL Effector Nucleases
Christian, M. L., Čermák, T., Doyle, E., Schmidt, C. L., Zhang, F., Hummel, A. W., Bogdanove, A. J., & Voytas, D. F. (2010) · Genetics · doi:10.1534/genetics.110.120717 · Cited by 2,033 · cited 2× · see citing works
“TALENs are custom-designed nucleases that consist of a modular DNA-binding domain fused to a monomeric, C-terminal FokI nuclease domain (Christian et al. 2010).”
BackgroundImproving CRISPR-Cas nuclease specificity using truncated guide RNAs
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BackgroundTALENs: A widely applicable technology for targeted genome editing
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“Gene insertion can occur following TALEN-induced cleavage, via homologous recombination, or NHEJ mechanisms (reviewed in Gaj et al. 2013 and Joung and Sander 2013).”
BackgroundThe Genome of the Sea Urchin <i>Strongylocentrotus purpuratus</i>
Sodergren, E., Weinstock, G. M., Davidson, E. H., Cameron, R. A., Gibbs, R. A., Angerer, R. C., Angerer, L. M., Arnone, M. I., Burgess, D. R., Burke, R. D., et al. (2006) · Science · doi:10.1126/science.1133609 · Cited by 1,132 · cited 2× · see citing works
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BackgroundIn vivo genome editing using a high-efficiency TALEN system
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A novel TALE nuclease scaffold enables high genome editing activity in combination with low toxicity
Mussolino, C., Morbitzer, R., Lütge, F., Dannemann, N., Lahaye, T., & Cathomen, T. (2011) · Nucleic Acids Research · doi:10.1093/nar/gkr597 · Cited by 714 · cited 2× · see citing works
“Transcription/translation in vitro cleavage assay TALEN efficacy was evaluated using an in vitro DNA cleavage assay [referred to here as transcription/translation (TnT) assay] similar to that previously described (Mussolino et al. 2011).”
MethodHeritable gene targeting in zebrafish using customized TALENs
Huang, P., Xiao, A., Zhou, M., Zhu, Z., Lin, S., & Zhang, B. (2011) · Nature Biotechnology · doi:10.1038/nbt.1939 · Cited by 658 · cited 2× · see citing works
“These indels can be up to several hundred base pairs in length and result in frameshift mutations that lead to the production of truncated or nonfunctional proteins (Sander et al. 2011; Lei et al. 2012; Ansai et al. 2013).”
BackgroundInsights into bilaterian evolution from three spiralian genomes
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“…TALENs featuring two alternative backbone architectures [either with long N- and C-terminal domains (N287/C230) and the wild-type FokI domain from the pTAL3 vector (Cermak et al. 2011) or with the shorter backbone architecture (N136/C63) with heterodimeric DD/RR FokI domains (Dahlem et al. 2012).”
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Auer, T. O., Duroure, K., De Cian, A., Concordet, J.-P., & Del Bene, F. (2014) · Genome Research · doi:10.1101/gr.161638.113 · Cited by 631 · cited 1× · see citing works
“Furthermore, as both systems use very different initial mechanisms of DNA sequence recognition, it is possible that in some cases where one system fails or is inefficient, the other might work better (Hwang et al. 2013; Auer et al. 2014).”
BackgroundTargeted gene disruption in somatic zebrafish cells using engineered TALENs
Sander, J. D., Cade, L., Khayter, C., Reyon, D., Peterson, R. T., Joung, J. K., & Yeh, J.-R. J. (2011) · Nature Biotechnology · doi:10.1038/nbt.1934 · Cited by 622 · cited 2× · see citing works
“These indels can be up to several hundred base pairs in length and result in frameshift mutations that lead to the production of truncated or nonfunctional proteins (Sander et al. 2011; Lei et al. 2012; Ansai et al. 2013).”
BackgroundTAL Effector-Nucleotide Targeter (TALE-NT) 2.0: tools for TAL effector design and target prediction
Doyle, E., Booher, N. J., Standage, D., Voytas, D. F., Brendel, V., VanDyk, J. K., & Bogdanove, A. J. (2012) · Nucleic Acids Research · doi:10.1093/nar/gks608 · Cited by 587 · cited 1× · see citing works
“0 and 2.0; https://tale-nt.cac.cornell.edu/) (Doyle et al. 2012).”
InfluentialEfficient TALEN-mediated gene knockout in livestock
Carlson, D. F., Tan, W. S., Lillico, S. G., Štveráková, D., Proudfoot, C., Christian, M. L., Voytas, D. F., Long, C. R., Whitelaw, B., & Fahrenkrug, S. C. (2012) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1211446109 · Cited by 580 · cited 1× · see citing works
“Compared with long-range deletions, inversion events are rare and have only been reported in vivo at rates of 0.2– 1% and 4% in zebrafish (Gupta et al. 2013; Xiao et al. 2013) and pigs (Carlson et al. 2012), respectively.”
BackgroundInfluentialDenes, A. S., Jékely, G., Steinmetz, P. R. H., Raible, F., Snyman, H., Prud'homme, B., Ferrier, D. E. K., Balavoine, G., & Arendt, D. (2007) · Cell · doi:10.1016/j.cell.2007.02.040 · Cited by 480 · cited 2× · see citing works
“…(Conzelmann et al. 2011), and treatment with small-molecule activators and inhibitors, to directly modulate protein functions in vitro (estrogen receptor) (Keay and Thornton 2009) and in vivo (Denes et al. 2007; Schneider and Bowerman 2007; Dray et al. 2010; Demilly et al. 2013; Lidke et al. 2014).”
BackgroundMethodSelf-citationDahlem, T. J., Hoshijima, K., Jurynec, M. J., Gunther, D., Starker, C. G., Locke, A. S., Weis, A. M., Voytas, D. F., & Grunwald, D. J. (2012) · PLoS Genetics · doi:10.1371/journal.pgen.1002861 · Cited by 453 · cited 6× · see citing works
“TALEN efficiency in vivo is reported to be significantly decreased where mismatches are present in one or both of the TALEN recognition sequences (Cade et al. 2012; Dahlem et al. 2012).”
BackgroundMethodInfluentialChromosomal deletions and inversions mediated by TALENs and CRISPR/Cas in zebrafish
Xiao, A., Wang, Z., Hu, Y., Wu, Y., Luo, Z., Yang, Z., Zu, Y., Li, W., Huang, P., Tong, X., et al. (2013) · Nucleic Acids Research · doi:10.1093/nar/gkt464 · Cited by 388 · cited 2× · see citing works
“This variety of mutations is similar to what has been shown for TALEN-mediated in vivo genome modification studies in other model systems (Ma et al. 2012; Qiu et al. 2013; Xiao et al. 2013).”
BackgroundResultInfluentialTessmar-Raible, K., Raible, F., Christodoulou, F., Guy, K., Rembold, M., Hausen, H., & Arendt, D. (2007) · Cell · doi:10.1016/j.cell.2007.04.041 · Cited by 384 · cited 1× · see citing works
“…circadian light receptor (Zantke et al. 2013); estrogen receptor (er), a verified ortholog of vertebrate liganddependent estrogen receptors (Keay and Thornton 2009); and vasotocin-neurophysin (vtn), an ortholog of oxytocin/ vasopressin-neurophysin prepro-hormones (Tessmar-Raible et al. 2007).”
BackgroundInversion of dorsoventral axis?
Arendt, D., & Nübler‐Jung, K. (1994) · Nature · doi:10.1038/371026a0 · Cited by 334 · cited 1× · see citing works
“Evidence from comparative morphology and development suggest that nereidid annelids like P. dumerilii possess a body plan that is likely ancestral for Bilateria (Dohrn 1875; Arendt and Nübler-Jung 1994, 1997; Tessmar-Raible and Arendt 2003).”
BackgroundTALEN-mediated precise genome modification by homologous recombination in zebrafish
Zu, Y., Tong, X., Wang, Z., Liu, D., Pan, R., Li, Z., Hu, Y., Luo, Z., Huang, P., Wu, Q., et al. (2013) · Nature Methods · doi:10.1038/nmeth.2374 · Cited by 328 · cited 1× · see citing works
“…mice (Davies et al. 2013; Qiu et al. 2013; Wang et al. 2013), teleost fish [zebrafish (Danio rerio)] (Huang et al. 2011; Bedell et al. 2012; Zu et al. 2013), medaka (Oryzias latipes) (Ansai et al. 2013, 2014), Xenopus (Ishibashi et al. 2012; Lei et al. 2012; Suzuki et al. 2013), and D.…”
Tomer, R., Denes, A. S., Tessmar-Raible, K., & Arendt, D. (2010) · Cell · doi:10.1016/j.cell.2010.07.043 · Cited by 317 · cited 1× · see citing works
“These have been facilitated by reliable techniques such as in situ hybridization (Tessmar-Raible et al. 2005), quantitative PCR (Dray et al. 2010; Zantke et al. 2013), and image registration (Tomer et al. 2010).”
MethodVertebrate-Type Intron-Rich Genes in the Marine Annelid <i>Platynereis dumerilii</i>
Raible, F., Tessmar-Raible, K., Osoegawa, K., Wincker, P., Jubin, C., Balavoine, G., Ferrier, D., Benes, V., de Jong, P., Weissenbach, J., et al. (2005) · Science · doi:10.1126/science.1119089 · Cited by 257 · cited 3× · see citing works
“These have been facilitated by reliable techniques such as in situ hybridization (Tessmar-Raible et al. 2005), quantitative PCR (Dray et al. 2010; Zantke et al. 2013), and image registration (Tomer et al. 2010).”
BackgroundMethodCade, L., Reyon, D., Hwang, W. Y., Tsai, S. Q., Patel, S., Khayter, C., Joung, J. K., Sander, J. D., Peterson, R. T., & Yeh, J.-R. J. (2012) · Nucleic Acids Research · doi:10.1093/nar/gks518 · Cited by 243 · cited 6× · see citing works
“Genetics Toolbox Review 83 Germline transmission of TALEN-induced mutations In a variety of animal models, TALEN-induced mutations have been shown to occur in the germline, thus facilitating the establishment of mutant lines (Cade et al. 2012; Aryan et al. 2013; Qiu et al. 2013; Sajwan et al. 2013).”
BackgroundMethodInfluentialLei, Y., Guo, X., Liu, Y., Cao, Y., Deng, Y., Chen, X., Cheng, C. H. K., Dawid, I. B., Chen, Y., & Zhao, H. (2012) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1215421109 · Cited by 242 · cited 3× · see citing works
“…2013; Qiu et al. 2013; Wang et al. 2013), teleost fish [zebrafish (Danio rerio)] (Huang et al. 2011; Bedell et al. 2012; Zu et al. 2013), medaka (Oryzias latipes) (Ansai et al. 2013, 2014), Xenopus (Ishibashi et al. 2012; Lei et al. 2012; Suzuki et al. 2013), and D. melanogaster (Liu et al. 2012).”
BackgroundThe normal development of Platynereis dumerilii (Nereididae, Annelida)
Fischer, A., Henrich, T., & Arendt, D. (2010) · Frontiers in Zoology · doi:10.1186/1742-9994-7-31 · Cited by 235 · cited 1× · see citing works
“Additionally, Platynereis is also studied to understand principles of animal development (reviewed in Fischer et al. 2010), the hormonal regulation of regeneration vs. maturation (Hauenschild 1974; Hofmann 1976), and chronobiology (Hauenschild 1960; Zantke et al. 2013).”
BackgroundEfficient and Specific Modifications of the Drosophila Genome by Means of an Easy TALEN Strategy
Liu, J., Li, C., Yu, Z., Huang, P., Wu, H., Wei, C., Zhu, N., Shen, Y., Chen, Y., Zhang, B., et al. (2012) · Journal of genetics and genomics/Journal of Genetics and Genomics · doi:10.1016/j.jgg.2012.04.003 · Cited by 209 · cited 3× · see citing works
“…2013; Qiu et al. 2013; Wang et al. 2013), teleost fish [zebrafish (Danio rerio)] (Huang et al. 2011; Bedell et al. 2012; Zu et al. 2013), medaka (Oryzias latipes) (Ansai et al. 2013, 2014), Xenopus (Ishibashi et al. 2012; Lei et al. 2012; Suzuki et al. 2013), and D. melanogaster (Liu et al. 2012).”
BackgroundArendt, D., & Nübler‐Jung, K. (1997) · Mechanisms of Development · doi:10.1016/s0925-4773(96)00620-x · Cited by 181 · cited 1× · see citing works
“Evidence from comparative morphology and development suggest that nereidid annelids like P. dumerilii possess a body plan that is likely ancestral for Bilateria (Dohrn 1875; Arendt and Nübler-Jung 1994, 1997; Tessmar-Raible and Arendt 2003).”
BackgroundHighly Efficient and Specific Genome Editing in Silkworm Using Custom TALENs
Ma, S., Zhang, S., Wang, F., Liu, Y., Liu, Y., Xu, H., Liu, C., Lin, Y., Zhao, P., & Xia, Q. (2012) · PLoS ONE · doi:10.1371/journal.pone.0045035 · Cited by 168 · cited 4× · see citing works
“…2013; Qiu et al. 2013; Wang et al. 2013), teleost fish [zebrafish (Danio rerio)] (Huang et al. 2011; Bedell et al. 2012; Zu et al. 2013), medaka (Oryzias latipes) (Ansai et al. 2013, 2014), Xenopus (Ishibashi et al. 2012; Lei et al. 2012; Suzuki et al. 2013), and D. melanogaster (Liu et al. 2012).”
BackgroundResultInfluentialConserved MIP receptor–ligand pair regulates <i>Platynereis</i> larval settlement
Conzelmann, M., Williams, E. A., Tunaru, S., Randel, N., Shahidi, R., Asadulina, A., Berger, J., Offermanns, S., & Jékely, G. (2013) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1220285110 · Cited by 155 · cited 2× · see citing works
“While useful for gaining spatio-temporal insight into protein activity, the penetration (i.e., effective dosage), affinity, and specificity of smallmolecule inhibitors (often developed for use in other systems) may limit the pathways and targets that can be investigated in vivo in Platynereis.”
BackgroundMethodCircadian and circalunar clock interactions in a marine annelid
Zantke, J., Ishikawa-Fujiwara, T., Arboleda, E., Lohs, C., Schipany, K., Hallay, N., Straw, A. D., Todo, T., & Tessmar-Raible, K. (2013) · Cell Reports · doi:10.1016/j.celrep.2013.08.031 · Cited by 148 · cited 6× · see citing works
“These have been facilitated by reliable techniques such as in situ hybridization (Tessmar-Raible et al. 2005), quantitative PCR (Dray et al. 2010; Zantke et al. 2013), and image registration (Tomer et al. 2010).”
BackgroundMethodInfluentialSelf-citationWatanabe, T., Ochiai, H., Sakuma, T., Horch, H. W., Hamaguchi, N., Nakamura, T., Bando, T., Ohuchi, H., Yamamoto, T., Noji, S., et al. (2012) · Nature Communications · doi:10.1038/ncomms2020 · Cited by 139 · cited 2× · see citing works
“…functional in a variety of other invertebrate arthropods, including mosquitos (Aedes aegypti) (Aryan et al. 2013) and Anopheles gambiae (Smidler et al. 2013), silkworm (Bombyx mori) (Ma et al. 2012; Sajwan et al. 2013; Takasu et al. 2013), and cricket (Gryllus bimaculatus) (Watanabe et al. 2012).”
BackgroundNeuropeptides regulate swimming depth of Platynereis larvae
Conzelmann, M., Offenburger, S.-L., Asadulina, A., Keller, T., Münch, T. A., & Jékely, G. (2011) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1109085108 · Cited by 136 · cited 2× · see citing works
“In addition, generating celltype-specific transgenic lines is not trivial, considering that only few specific enhancers have been characterized for Platynereis.”
BackgroundMethodHauenschild, C. (1960) · Cold Spring Harbor Symposia on Quantitative Biology · doi:10.1101/sqb.1960.025.01.051 · Cited by 134 · cited 1× · see citing works
“Additionally, Platynereis is also studied to understand principles of animal development (reviewed in Fischer et al. 2010), the hormonal regulation of regeneration vs. maturation (Hauenschild 1974; Hofmann 1976), and chronobiology (Hauenschild 1960; Zantke et al. 2013).”
BackgroundKeay, J., & Thornton, J. W. (2008) · Endocrinology · doi:10.1210/en.2008-1338 · Cited by 130 · cited 5× · see citing works
“…known SNPs or exon–intron boundaries; (2) target sites were upstream of, or within critical protein functional domains; (3) a “T” residue preceded the first nucleotide in each TALENbinding site; and (4) spacer regions contained a unique restriction site to facilitate mutation screening by…”
BackgroundMethodInfluentialEmerging systems: between vertebrates and arthropods, the Lophotrochozoa
Tessmar‐Raible, K. (2003) · Current Opinion in Genetics & Development · doi:10.1016/s0959-437x(03)00086-8 · Cited by 124 · cited 1× · see citing works
“Evidence from comparative morphology and development suggest that nereidid annelids like P. dumerilii possess a body plan that is likely ancestral for Bilateria (Dohrn 1875; Arendt and Nübler-Jung 1994, 1997; Tessmar-Raible and Arendt 2003).”
BackgroundSchneider, S., & Bowerman, B. A. (2007) · Developmental Cell · doi:10.1016/j.devcel.2007.05.002 · Cited by 123 · cited 1× · see citing works
“…(Conzelmann et al. 2011), and treatment with small-molecule activators and inhibitors, to directly modulate protein functions in vitro (estrogen receptor) (Keay and Thornton 2009) and in vivo (Denes et al. 2007; Schneider and Bowerman 2007; Dray et al. 2010; Demilly et al. 2013; Lidke et al. 2014).”
BackgroundAnsai, S., Sakuma, T., Yamamoto, T., Ariga, H., Uemura, N., Takahashi, R., & Kinoshita, M. (2013) · Genetics · doi:10.1534/genetics.112.147645 · Cited by 115 · cited 3× · see citing works
“…2013; Qiu et al. 2013; Wang et al. 2013), teleost fish [zebrafish (Danio rerio)] (Huang et al. 2011; Bedell et al. 2012; Zu et al. 2013), medaka (Oryzias latipes) (Ansai et al. 2013, 2014), Xenopus (Ishibashi et al. 2012; Lei et al. 2012; Suzuki et al. 2013), and D. melanogaster (Liu et al. 2012).”
BackgroundTALEN-mediated editing of the mouse Y chromosome
Wang, H., Hu, Y., Markoulaki, S., Welstead, G. G., Cheng, A. W., Shivalila, C., Pyntikova, T., Dadon, D. B., Voytas, D. F., Bogdanove, A. J., et al. (2013) · Nature Biotechnology · doi:10.1038/nbt.2595 · Cited by 113 · cited 3× · see citing works
“…use of TALENs for inducing targeted mutations has been reported in many conventional models, for example: mice (Davies et al. 2013; Qiu et al. 2013; Wang et al. 2013), teleost fish [zebrafish (Danio rerio)] (Huang et al. 2011; Bedell et al. 2012; Zu et al. 2013), medaka (Oryzias latipes) (Ansai et…”
BackgroundTALEN-Based Gene Disruption in the Dengue Vector Aedes aegypti
Aryan, A., Anderson, M. A. E., Myles, K. M., & Adelman, Z. N. (2013) · PLoS ONE · doi:10.1371/journal.pone.0060082 · Cited by 110 · cited 4× · see citing works
“TALENs are also reported to be functional in a variety of other invertebrate arthropods, including mosquitos (Aedes aegypti) (Aryan et al. 2013) and Anopheles gambiae (Smidler et al. 2013), silkworm (Bombyx mori) (Ma et al. 2012; Sajwan et al. 2013; Takasu et al. 2013), and cricket (Gryllus…”
BackgroundInfluentialTargeted chromosomal deletions and inversions in zebrafishPreprint
Gupta, A., Hall, V. L., Kok, F. O., Shin, M., McNulty, J. C., Lawson, N. D., & Wolfe, S. A. (2013) · Genome Research · doi:10.1101/gr.154070.112 · Cited by 103 · cited 3× · see citing works
“Note added in proof: See Zantke et al. 2014 (pp. 19–31) in this issue for a related work.”
BackgroundInfluentialThe CRISPR System—Keeping Zebrafish Gene Targeting Fresh
Blackburn, P. R., Campbell, J. M., Clark, K. J., & Ekker, S. C. (2013) · Zebrafish · doi:10.1089/zeb.2013.9999 · Cited by 101 · cited 1× · see citing works
“While the production of Cas9/CRISPR reagents is more straightforward than constructing TALENs and thus potentially useful for multiplex functional screens, possible target sites are more limited than in the case of TALENs (reviewed in Blackburn et al. 2013).”
BackgroundHedgehog Signaling Regulates Segment Formation in the Annelid Platynereis
Dray, N., Tessmar‐Raible, K., Gouar, M. L., Vibert, L., Christodoulou, F., Schipany, K., Guillou, A., Zantke, J., Snyman, H., Béhague, J., et al. (2010) · Science · doi:10.1126/science.1188913 · Cited by 98 · cited 3× · see citing works
“…(Conzelmann et al. 2011), and treatment with small-molecule activators and inhibitors, to directly modulate protein functions in vitro (estrogen receptor) (Keay and Thornton 2009) and in vivo (Denes et al. 2007; Schneider and Bowerman 2007; Dray et al. 2010; Demilly et al. 2013; Lidke et al. 2014).”
BackgroundMethodTessmar‐Raible, K., Steinmetz, P. R. H., Snyman, H., Hassel, M., & Arendt, D. (2005) · BioTechniques · doi:10.2144/000112023 · Cited by 98 · cited 1× · see citing works
“These have been facilitated by reliable techniques such as in situ hybridization (Tessmar-Raible et al. 2005), quantitative PCR (Dray et al. 2010; Zantke et al. 2013), and image registration (Tomer et al. 2010).”
MethodBackfisch, B., Veedin Rajan, V. B., Fischer, R. M., Lohs, C., Arboleda, E., Tessmar-Raible, K., & Raible, F. (2013) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1209657109 · Cited by 93 · cited 3× · see citing works
“Established techniques for transgenesis also provide the opportunity to label specific cell types (Backfisch et al. 2013).”
BackgroundMethodQiu, Z., Liu, M., Chen, Z., Shao, Y., Pan, H., Wei, G., Yu, C., Zhang, L., Li, X., Wang, P., et al. (2013) · Nucleic Acids Research · doi:10.1093/nar/gkt258 · Cited by 89 · cited 3× · see citing works
“This variety of mutations is similar to what has been shown for TALEN-mediated in vivo genome modification studies in other model systems (Ma et al. 2012; Qiu et al. 2013; Xiao et al. 2013).”
BackgroundResultEfficient TALEN Construction for Bombyx mori Gene Targeting
Takasu, Y., Sajwan, S., Daimon, T., Osanai-Futahashi, M., Uchino, K., Sezutsu, H., Tamura, T., & Žurovec, M. (2013) · PLoS ONE · doi:10.1371/journal.pone.0073458 · Cited by 87 · cited 2× · see citing works
“…2013; Qiu et al. 2013; Wang et al. 2013), teleost fish [zebrafish (Danio rerio)] (Huang et al. 2011; Bedell et al. 2012; Zu et al. 2013), medaka (Oryzias latipes) (Ansai et al. 2013, 2014), Xenopus (Ishibashi et al. 2012; Lei et al. 2012; Suzuki et al. 2013), and D. melanogaster (Liu et al. 2012).”
BackgroundTargeted Mutagenesis in the Malaria Mosquito Using TALE Nucleases
Smidler, A. L., Terenzi, O., Soichot, J., Levashina, E. A., & Marois, É. (2013) · PLoS ONE · doi:10.1371/journal.pone.0074511 · Cited by 86 · cited 2× · see citing works
“…2013; Qiu et al. 2013; Wang et al. 2013), teleost fish [zebrafish (Danio rerio)] (Huang et al. 2011; Bedell et al. 2012; Zu et al. 2013), medaka (Oryzias latipes) (Ansai et al. 2013, 2014), Xenopus (Ishibashi et al. 2012; Lei et al. 2012; Suzuki et al. 2013), and D. melanogaster (Liu et al. 2012).”
BackgroundEfficient disruption of endogenous Bombyx gene by TAL effector nucleases
Sajwan, S., Takasu, Y., Tamura, T., Uchino, K., Sezutsu, H., & Žurovec, M. (2012) · Insect Biochemistry and Molecular Biology · doi:10.1016/j.ibmb.2012.10.011 · Cited by 82 · cited 3× · see citing works
“…2013; Qiu et al. 2013; Wang et al. 2013), teleost fish [zebrafish (Danio rerio)] (Huang et al. 2011; Bedell et al. 2012; Zu et al. 2013), medaka (Oryzias latipes) (Ansai et al. 2013, 2014), Xenopus (Ishibashi et al. 2012; Lei et al. 2012; Suzuki et al. 2013), and D. melanogaster (Liu et al. 2012).”
BackgroundAnsai, S., Inohaya, K., Yoshiura, Y., Schartl, M., Uemura, N., Takahashi, R., & Kinoshita, M. (2013) · Development Growth & Differentiation · doi:10.1111/dgd.12104 · Cited by 82 · cited 2× · see citing works
“…2013; Qiu et al. 2013; Wang et al. 2013), teleost fish [zebrafish (Danio rerio)] (Huang et al. 2011; Bedell et al. 2012; Zu et al. 2013), medaka (Oryzias latipes) (Ansai et al. 2013, 2014), Xenopus (Ishibashi et al. 2012; Lei et al. 2012; Suzuki et al. 2013), and D. melanogaster (Liu et al. 2012).”
BackgroundInvolvement of the Wnt/β-catenin pathway in neurectoderm architecture in Platynereis dumerilii
Demilly, A., Steinmetz, P. R. H., Gazave, E., Marchand, L., & Vervoort, M. (2013) · Nature Communications · doi:10.1038/ncomms2915 · Cited by 78 · cited 1× · see citing works
“…(Conzelmann et al. 2011), and treatment with small-molecule activators and inhibitors, to directly modulate protein functions in vitro (estrogen receptor) (Keay and Thornton 2009) and in vivo (Denes et al. 2007; Schneider and Bowerman 2007; Dray et al. 2010; Demilly et al. 2013; Lidke et al. 2014).”
BackgroundSuzuki, K., Isoyama, Y., Kashiwagi, K., Sakuma, T., Ochiai, H., Sakamoto, N., Furuno, N., Kashiwagi, A., & Yamamoto, T. (2013) · Biology Open · doi:10.1242/bio.20133855 · Cited by 77 · cited 3× · see citing works
“…2013; Qiu et al. 2013; Wang et al. 2013), teleost fish [zebrafish (Danio rerio)] (Huang et al. 2011; Bedell et al. 2012; Zu et al. 2013), medaka (Oryzias latipes) (Ansai et al. 2013, 2014), Xenopus (Ishibashi et al. 2012; Lei et al. 2012; Suzuki et al. 2013), and D. melanogaster (Liu et al. 2012).”
BackgroundHighly efficient bi-allelic mutation rates using TALENs in Xenopus tropicalis
Ishibashi, S., Cliffe, R. N., & Amaya, E. (2012) · Biology Open · doi:10.1242/bio.20123228 · Cited by 71 · cited 5× · see citing works
“Suzuki, K. T., Y. Isoyama, K. Kashiwagi, T. Sakuma, H. Ochiai et al., 2013 High efficiency TALENs enable F0 functional analysis by targeted gene disruption in Xenopus laevis embryos.”
InfluentialGenetic and Genomic Tools for the Marine AnnelidPlatynereis dumerilii
Zantke, J., Bannister, S. C., Rajan, V. B. V., Raible, F., & Tessmar‐Raible, K. (2014) · Genetics · doi:10.1534/genetics.112.148254 · Cited by 66 · cited 5× · see citing works
“See also Tables 1 and 2.”
BackgroundMethodInfluentialDavies, B., Davies, G., Preece, C., Puliyadi, R., Szumska, D., & Bhattacharya, S. (2013) · PLoS ONE · doi:10.1371/journal.pone.0060216 · Cited by 63 · cited 2× · see citing works
“Successful use of TALENs for inducing targeted mutations has been reported in many conventional models, for example: mice (Davies et al. 2013; Qiu et al. 2013; Wang et al. 2013), teleost fish [zebrafish (Danio rerio)] (Huang et al. 2011; Bedell et al. 2012; Zu et al. 2013), medaka (Oryzias latipes)…”
BackgroundLong Noncoding RNAs in Development: Solidifying the Lncs to Hox Gene Regulation
Dasen, J. S. (2013) · Cell Reports · doi:10.1016/j.celrep.2013.09.033 · Cited by 45 · see citing works
Regeneration and endocrinology in the polychaetePlatynereis dumerilii
Hofmann, D. K. (1976) · Development Genes and Evolution · doi:10.1007/bf00848884 · Cited by 30 · cited 1× · see citing works
“Additionally, Platynereis is also studied to understand principles of animal development (reviewed in Fischer et al. 2010), the hormonal regulation of regeneration vs. maturation (Hauenschild 1974; Hofmann 1976), and chronobiology (Hauenschild 1960; Zantke et al. 2013).”
BackgroundLidke, A. K., Bannister, S. C., Löwer, A., Apel, D., Podleschny, M., Kollmann, M., Ackermann, C., García‐Alonso, J., Raible, F., & Rebscher, N. (2013) · General and Comparative Endocrinology · doi:10.1016/j.ygcen.2013.11.017 · Cited by 20 · cited 1× · see citing works
“…(Conzelmann et al. 2011), and treatment with small-molecule activators and inhibitors, to directly modulate protein functions in vitro (estrogen receptor) (Keay and Thornton 2009) and in vivo (Denes et al. 2007; Schneider and Bowerman 2007; Dray et al. 2010; Demilly et al. 2013; Lidke et al. 2014).”
BackgroundSimakov, O., Larsson, T. A., & Arendt, D. (2012) · Briefings in Functional Genomics · doi:10.1093/bfgp/els049 · Cited by 16 · cited 2× · see citing works
“(Sodergren et al. 2006; Srivastava et al. 2010; Simakov et al. 2013b).”
BackgroundConditional and specific cell ablation in the marine annelid Platynereis dumerilii
Veedin-Rajan, V. B., Fischer, R. M., Raible, F., & Tessmar-Raible, K. (2013) · PLoS ONE · doi:10.1371/journal.pone.0075811 · Cited by 15 · cited 2× · see citing works
“This approach has already contributed to the discovery of r-opsin1+ peripheral photoreceptor cells (Backfisch et al. 2013).”
Background[Endocrine control of sexual development in some polychaetes (author's transl)].
Hauenschild, C. (1974) · PubMed · Cited by 3 · see citing works
Flagged first, then by citations
Research works printed but not linked: 2
- Hauenschild C., 1974 Endocrine control of sexual development in some polychaetes (in German). Fortschr. Zool. 22: 75– 92. 4426570
- Hauenschild C. Fischer A., 1969 pp. 1– 55 in Platynereis dumerilii. Mikroskopische Anatomie, Fortpflanzung, Entwicklung [Platynereis dumerilii. Microscopical Anatomy, Reproduction and Development]. Groses Zoologisches Praktikum Heft, Stuttgart, Germany.
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Sorted by citations
Current status of annelid phylogeny
Weigert, A., & Bleidorn, C. (2016) · Organisms Diversity & Evolution · doi:10.1007/s13127-016-0265-7 · Cited by 213 · see citing works
“…worms have been established, as e.g., in situ hybridization, RNA interference, Morpholino knockdowns, transgenic lineages, or gene modification by transcriptional activatorlike effector nucleases (TALENs) (Bannister et al. 2014; Zantke et al. 2014; Backfisch et al. 2013; Backfisch et al. 2014).”
BackgroundGene editing for cell engineering: trends and applications
Gupta, S. K., & Shukla, P. (2016) · Critical Reviews in Biotechnology · doi:10.1080/07388551.2016.1214557 · Cited by 130 · see citing works
An Overview of Monthly Rhythms and Clocks
Raible, F., Takekata, H., & Tessmar-Raible, K. (2017) · Frontiers in Neurology · doi:10.3389/fneur.2017.00189 · Cited by 121 · see citing works
“More recently, transgenesis and genome mutagenesis have allowed the generation of knockout strains for specific genes (49).”
BackgroundMethodFunctional genetics for all: engineered nucleases, CRISPR and the gene editing revolution
Gilles, A. F., & Averof, M. (2014) · EvoDevo · doi:10.1186/2041-9139-5-43 · Cited by 110 · see citing works
Making designer mutants in model organisms
Peng, Y., Clark, K. J., Campbell, J. M., Panetta, M. R., Guo, Y., & Ekker, S. C. (2014) · Development · doi:10.1242/dev.102186 · Cited by 101 · see citing works
Spectral Tuning of Phototaxis by a Go-Opsin in the Rhabdomeric Eyes of Platynereis
Gühmann, M., Jia, H., Randel, N., Verasztó, C., Bezares-Calderón, L. A., Michiels, N. K., Yokoyama, S., & Jékely, G. (2015) · Current Biology · doi:10.1016/j.cub.2015.07.017 · Cited by 101 · see citing works
“Here, we characterize a Go-opsin from the marine annelid Platynereis dumerilii [3–5, 12–15].”
Neural circuitry of a polycystin-mediated hydrodynamic startle response for predator avoidance
Bezares-Calderón, L. A., Berger, J., Jasek, S., Verasztó, C., Mendes, S., Gühmann, M., Almeda, R., Shahidi, R., & Jékely, G. (2018) · eLife · doi:10.7554/elife.36262 · Cited by 88 · see citing works
Lai, A. G., & Aboobaker, A. A. (2017) · Developmental Biology · doi:10.1016/j.ydbio.2017.10.010 · Cited by 84 · see citing works
“Currently Platynereis, capable of posterior regeneration involving GMP expressing mesodermal and ectodermal cells, is the most advanced genetic system (Gazave et al. 2013; Bannister et al. 2014; Zantke et al. 2014),”
BackgroundVerasztó, C., Gühmann, M., Jia, H., Rajan, V. B. V., Bezares-Calderón, L. A., López, C. P., Randel, N., Shahidi, R., Michiels, N. K., Yokoyama, S., et al. (2018) · eLife · doi:10.7554/elife.36440 · Cited by 79 · see citing works
Gene editing of DNAH11 restores normal cilia motility in primary ciliary dyskinesia
Lai, M., Pifferi, M., Bush, A., Piras, M., Michelucci, A., Cicco, M. D., Grosso, A. D., Quaranta, P., Cursi, C., Tantillo, E., et al. (2016) · Journal of Medical Genetics · doi:10.1136/jmedgenet-2015-103539 · Cited by 73 · see citing works
Marine metagenomics as a source for bioprospecting
Kodzius, R., & Gojobori, T. (2015) · Marine Genomics · doi:10.1016/j.margen.2015.07.001 · Cited by 73 · see citing works
“…to genetically modi fi ed sea anemone Nematostella vectensis (Ikmi et al., 2014), sea urchin embryos (Hosoi et al., 2014), amphioxus Branchiostoma belcheri (G. Li et al., 2014), marine annelid Platynereis dumerilii (Bannister et al., 2014) and ascidian Ciona intestinalis (Yoshida et al., 2014).”
BackgroundGenetic and Genomic Tools for the Marine AnnelidPlatynereis dumerilii
Zantke, J., Bannister, S. C., Rajan, V. B. V., Raible, F., & Tessmar‐Raible, K. (2014) · Genetics · doi:10.1534/genetics.112.148254 · Cited by 66 · see citing works
“(See Table 1.)”
BackgroundMethodInfluentialGenome Editing Using TALENs in Blind Mexican Cavefish, Astyanax mexicanus
Ma, L., Jeffery, W. R., Essner, J. J., & Kowalko, J. E. (2015) · PLoS ONE · doi:10.1371/journal.pone.0119370 · Cited by 65 · see citing works
A serial multiplex immunogold labeling method for identifying peptidergic neurons in connectomes
Shahidi, R., Williams, E. A., Conzelmann, M., Asadulina, A., Verasztó, C., Jasek, S., Bezares-Calderón, L. A., & Jékely, G. (2015) · eLife · doi:10.7554/elife.11147 · Cited by 65 · see citing works
Creating cell and animal models of human disease by genome editing using CRISPR/Cas9
Zarei, A., Razban, V., Hosseini, S. E., & Tabei, K. (2019) · The Journal of Gene Medicine · doi:10.1002/jgm.3082 · Cited by 67 · see citing works
Козин, В. В., & Kostyuchenko, R. P. (2015) · Development Genes and Evolution · doi:10.1007/s00427-015-0496-1 · Cited by 62 · see citing works
“dumerilii (Backfisch et al. 2014; Bannister et al. 2014; Zantke et al. 2014), dissecting the exact role of GMP genes in polychaetes would be of great significance.”
BackgroundAndreatta, G., Broyart, C., Borghgraef, C., Vadiwala, K., Kozin, V., Polo, A., Bileck, A., Beets, I., Schoofs, L., Gerner, C., et al. (2020) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1910262116 · Cited by 61 · see citing works
“TALENs design and preparation were carried out as previously described (11).”
Self-citationFunctional role of pax6 during eye and nervous system development in the annelid Capitella teleta
Klann, M., & Seaver, E. C. (2019) · Developmental Biology · doi:10.1016/j.ydbio.2019.08.011 · Cited by 55 · see citing works
Aluru, N., Karchner, S. I., Franks, D. G., Nacci, D. E., Champlin, D. M., & Hahn, M. E. (2014) · Aquatic Toxicology · doi:10.1016/j.aquatox.2014.11.016 · Cited by 47 · see citing works
“…newts (Pleurodeles waltl) Tilapia (Oreochromis niloticus) Medaka (Oryzias melastigma) Yellow catfish (Pelteobagrus fulvidraco) (Lo et al., 2013) (Bannister et al., 2014) (Aryan et al., 2013b) (Aryan et al., 2013a) (Ma et al., 2012) (Takasu et al., 2013) (Sajwan et al., 2013) (Wang et al., 2013)…”
Chou, H.-C., Acevedo-Luna, N., Kuhlman, J. A., & Schneider, S. (2018) · BMC Genomics · doi:10.1186/s12864-018-4987-0 · Cited by 43 · see citing works
Rajan, V. B. V., Häfker, N. S., Arboleda, E., Poehn, B., Gossenreiter, T., Gerrard, E. J., Hofbauer, M., Mühlestein, C., Bileck, A., Gerner, C., et al. (2021) · Nature Ecology & Evolution · doi:10.1038/s41559-020-01356-1 · Cited by 40 · see citing works
Towards a systems-level understanding of development in the marine annelid Platynereis dumerilii
Williams, E. A., & Jékely, G. (2016) · Current Opinion in Genetics & Development · doi:10.1016/j.gde.2016.07.005 · Cited by 38 · see citing works
Old questions, new models: unraveling complex organ regeneration with new experimental approaches
Grillo, M., Konstantinides, N., & Averof, M. (2016) · Current Opinion in Genetics & Development · doi:10.1016/j.gde.2016.05.006 · Cited by 40 · see citing works
Poehn, B., Krishnan, S., Zurl, M., Ćorić, A., Rokvić, D., Häfker, N. S., Jaenicke, E., Arboleda, E., Orel, L., Raible, F., et al. (2022) · Nature Communications · doi:10.1038/s41467-022-32562-z · Cited by 40 · see citing works
Diving into marine genomics with CRISPR/Cas9 systems
Momose, T., & Concordet, J. (2016) · Marine Genomics · doi:10.1016/j.margen.2016.10.003 · Cited by 39 · see citing works
A scalable culturing system for the marine annelid Platynereis dumerilii
Kuehn, E., Stockinger, A. W., Girard, J., Raible, F., & Özpolat, B. D. (2019) · PLoS ONE · doi:10.1371/journal.pone.0226156 · Cited by 36 · see citing works
Positional RNA-Seq identifies candidate genes for phenotypic engineering of sexual traits
Arbore, R., Sekii, K., Beisel, C., Ladurner, P., Berezikov, E., & Schärer, L. (2015) · Frontiers in Zoology · doi:10.1186/s12983-015-0106-0 · Cited by 33 · see citing works
“[10–12]).”
Variation in spiralian development: insights from polychaetes
Seaver, E. C. (2014) · The International Journal of Developmental Biology · doi:10.1387/ijdb.140154es · Cited by 31 · see citing works
“In addition, recent development of reverse genetic tools using TALENs in Platynereis (Bannister et al., 2014), or use of CRISPR technology (Pennisi, 2013) holds great promise.”
BackgroundAnnelid genomes: Enchytraeus crypticus, a soil model for the innate (and primed) immune system
Amorim, M. J. B., Gansemans, Y., Gomes, S. I. L., Nieuwerburgh, F. V., & Scott-Fordsmand, J. J. (2021) · Lab Animal · doi:10.1038/s41684-021-00831-x · Cited by 28 · see citing works
Genome Editing of Structural Variations: Modeling and Gene Correction
Park, C., Sung, J. J., & Kim, D. (2016) · Trends in biotechnology · doi:10.1016/j.tibtech.2016.02.011 · Cited by 23 · see citing works
“Successful chromosomal inversions have also been reported in insect and annelid models using customized TALEN systems [36,37].”
MethodTools for Gene-Regulatory Analyses in the Marine Annelid Platynereis dumerilii
Backfisch, B., Козин, В. В., Kirchmaier, S., Tessmar‐Raible, K., & Raible, F. (2014) · PLoS ONE · doi:10.1371/journal.pone.0093076 · Cited by 23 · see citing works
“Consistent with this notion, Mos1-triggered non-homologous end-joining can lead to insertions and deletions in Caenorhabditis elegans [39]; likewise, our recent work indicates that in Platynereis genomic DNA, open double-strand breaks generated by engineered TALE nucleases can lead to insertions and deletions, presuma…”
BackgroundNon‐Mammalian Models for Neurohypophysial Peptides
Wircer, E., Ben‐Dor, S., & Levkowitz, G. (2016) · doi:10.1002/9781118760369.ch14 · Cited by 23 · see citing works
Revilla‐i‐Domingo, R., Rajan, V. B. V., Waldherr, M., Prohaczka, G., Musset, H., Orel, L., Gerrard, E. J., Smolka, M., Stockinger, A. W., Farlik, M., et al. (2021) · eLife · doi:10.7554/elife.66144 · Cited by 20 · see citing works
Towards an Understanding of Circatidal Clocks
Rock, A., Wilcockson, D. C., & Last, K. S. (2022) · Frontiers in Physiology · doi:10.3389/fphys.2022.830107 · Cited by 17 · see citing works
TMT-Opsins differentially modulate medaka brain function in a context-dependent manner
Fontinha, B. M., Zekoll, T., Al-Rawi, M., Gallach, M., Reithofer, F., Barker, A. J., Hofbauer, M., Fischer, R. M., von Haeseler, A., Baier, H., et al. (2021) · PLoS Biology · doi:10.1371/journal.pbio.3001012 · Cited by 16 · see citing works
“The final pCS2+ backbone vectors, containing the homodimeric FokI nuclease domains, were used as previously [65].”
Molecular circadian rhythms are robust in marine annelids lacking rhythmic behavior
Häfker, N. S., Holcik, L., Mat, A. M., Ćorić, A., Vadiwala, K., Beets, I., Stockinger, A. W., Atria, C. E., Hammer, S., Revilla‐i‐Domingo, R., et al. (2024) · PLoS Biology · doi:10.1371/journal.pbio.3002572 · Cited by 13 · see citing works
Marlétaz, F., Maeso, I., Faas, L., Isaacs, H. V., & Holland, P. W. H. (2015) · BMC Biology · doi:10.1186/s12915-015-0165-x · Cited by 15 · see citing works
“A powerful way to distinguish pre-duplication and post-duplication targets would be by comparison to Cdx target genes in non-vertebrates; however, for this, functional interference techniques will be needed in systems such as annelids, echinoderms, and cephalochordates [47].”
BackgroundGenome Editing in Astyanax mexicanus Using Transcription Activator-like Effector Nucleases (TALENs)
Kowalko, J. E., Ma, L., & Jeffery, W. R. (2016) · Journal of Visualized Experiments · doi:10.3791/54113 · Cited by 17 · see citing works
Pandey, D., Matsubara, T., Saito, T., Kazeto, Y., Gen, K., Sakuma, T., Yamamoto, T., Mekuchi, M., & Goto, R. (2021) · Journal of Marine Science and Engineering · doi:10.3390/jmse9121378 · Cited by 14 · see citing works
Feng, C., Li, X., Cui, H., Long, C., Liu, X., Xing-hua, T. I. A. N., Pan, D., & Luo, Y. (2016) · Journal of Integrative Agriculture · doi:10.1016/s2095-3119(16)61347-3 · Cited by 13 · see citing works
Circadian and Circalunar Clock Interactions and the Impact of Light in Platynereis dumerilii
Zantke, J., Oberlerchner, H., & Tessmar‐Raible, K. (2014) · Springer eBooks · doi:10.1007/978-4-431-55261-1_8 · Cited by 13 · see citing works
Molecular profiles, sources and lineage restrictions of stem cells in an annelid regeneration model
Stockinger, A. W., Adelmann, L., Fahrenberger, M., Ruta, C., Özpolat, B. D., Milivojev, N., Balavoine, G., & Raible, F. (2024) · Nature Communications · doi:10.1038/s41467-024-54041-3 · Cited by 10 · see citing works
Emerging trends in the study of spiralian larvae
Liang, Y., Carrillo‐Baltodano, A. M., & Martín-Durán, J. M. (2023) · Evolution & Development · doi:10.1111/ede.12459 · Cited by 9 · see citing works
“Genome editing technology via CRISPR/Cas9, TALENs, and zin ‐ finger ‐ nucleases is also becoming available for a handful of annelids (Bannister et al., 2014; Bezares ‐ Calderón et al., 2018; Guhmann et al., 2015; Klann & Seaver, 2019) and molluscs (Perry & Henry, 2015), expanding the possibilities…”
Fast cycling culture of the annelid model Platynereis dumerilii
Legras, M., Ghisleni, G., Regnard, L., Dias, M., Soilihi, R., Celmar, E., & Balavoine, G. (2023) · PLoS ONE · doi:10.1371/journal.pone.0295290 · Cited by 6 · see citing works
Dynamic microvilli sculpt bristles at nanometric scale
Ikeda, K. N., Belevich, I. N., Zelaya-Lainez, L., Orel, L., Füssl, J., Gumulec, J., Hellmich, C., Jokitalo, E., & Raible, F. (2024) · Nature Communications · doi:10.1038/s41467-024-48044-3 · Cited by 4 · see citing works
“Embryo collection and microinjections followed previously established procedures 34,35 .”
Clocks at sea: the genome-editing tide is rising
Kwiatkowski, E. R., Rosenthal, J. J. C., & Emery, P. (2024) · Trends in Genetics · doi:10.1016/j.tig.2024.01.006 · Cited by 4 · see citing works
“CKI-δ / ε inhibitors disrupted circadian locomotor rhythms but not circalunar spawning rhythms in the marine annelid Platynereis dumerilii , an emerging genetically tractable model for circalunar and circadian clock studies [71,72].”
Zelaya-Lainez, L., Balduzzi, G., Lahayne, O., Ikeda, K. N., Raible, F., Herzig, C., Nischkauer, W., Limbeck, A., & Hellmich, C. (2021) · JOM · doi:10.1007/s11837-021-04702-1 · Cited by 5 · see citing works
Assessment of Circalunar (~Monthly) Rhythms
Poehn, B., & Tessmar‐Raible, K. (2022) · Neuromethods · doi:10.1007/978-1-0716-2577-4_10 · Cited by 3 · see citing works
Lunar cycle-mediated behaviours in animals, including humans
Mandal, F. B. (2023) · Israel Journal of Ecology and Evolution · doi:10.1163/22244662-bja10060 · Cited by 3 · see citing works
Revilla‐i‐Domingo, R., Rajan, V. B. V., Waldherr, M., Prohaczka, G., Musset, H., Orel, L., Gerrard, E. J., Smolka, M., Farlik, M., Lucas, R. J., et al. (2021) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2021.01.10.426124 · Cited by 2 · see citing works
Reproductive Strategies in Annelida: Germ Cell Formation and Regeneration
Tadokoro, R. (2018) · Diversity and commonality in animals · doi:10.1007/978-4-431-56609-0_10 · Cited by 1 · see citing works
Klann, M., & Seaver, E. C. (2018) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/481135 · Cited by 1 · see citing works
“To date, only a few representatives within Lophotrochozoa have been successfully shown to be amenable for functional genomic studies, including the annelids Platynereis dumerilii (Bannister et al., 2014; Conzelmann et al., 2013) and Helobdella robusta (Song, Huang, Chang, & Weisblat, 2002), the mollusks, Tritia obsole…”
BackgroundAndreatta, G., Scaramuzza, F., Ćorić, A., Orel, L., & Tessmar‐Raible, K. (2025) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2025.03.12.642703 · Cited by 1 · see citing works
Behavioral rhythms are bad predictors of general organismal rhythmicityPreprint
Häfker, N. S., Holcik, L., Vadiwala, K., Beets, I., Mat, A. M., Stockinger, A. W., Hammer, S., Schoofs, L., Raible, F., & Tessmar‐Raible, K. (2022) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2022.07.16.500291
Andreatta, G., Scaramuzza, F., Ćorić, A., Orel, L., Mat, A. M., Takekata, H., Poehn, B., Milivojev, N., & Tessmar‐Raible, K. (2026) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2514719123 · Cited by 1 · see citing works
Exploiting Omic Data to Advance Predictive Ecotoxicology
Short, S., Etxabe, A. G., Swart, E., Rivetti, C., Campos, B., Krishnan, R., Kille, P., & Spurgeon, D. J. (2026) · Environmental Science & Technology · doi:10.1021/acs.est.6c01198
- Authors
- Bannister, S., Antonova, O., Polo, A., Lohs, C., Hallay, N., Valinciute, A., Raible, F., & Tessmar-Raible, K.
- Affiliations
- University of Vienna (AT)
- Max F. Perutz Laboratories, University of Vienna, 1030 Vienna, Austria
- Corresponding author
- Bannister, Stephanie
- ORCID
- Raible, Florian
- Venue
- Genetics
- Publisher
- Oxford University Press (OUP)
- Published
- 2014-05-01
- Volume
- 197
- Issue
- 1
- Pages
- 77-89
- ISSN
- 0016-6731 (ISSN-L)
- 1943-2631
- PMC
- PMC4012502
- OpenAlex
- W2121449726
- Cited by
- 56
- References
- 70
- Citations by year
- 2026: 2 · 2025: 1 · 2024: 4 · 2023: 3 · 2022: 4 · 2021: 7 · 2019: 4 · 2018: 5 · 2017: 2 · 2016: 10 · 2015: 7 · 2014: 7
- FWCI
- 3.55
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Licence
- academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model (vor)
- Locations
- Genetics (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Universität Wien
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Animal Genetics and Reproduction
- Keywords
- TALEN, Platynereis dumerilii, Polychaeta, targeted mutagenesis, frameshift mutation, inherited mutations, reverse genetics, genome editing
- MeSH terms
- Animals, Base Sequence, DNA Restriction Enzymes, Genetic Engineering, Larva, Molecular Sequence Data, Mutation, Polychaeta, Zygote, Reproducibility of Results, Sequence Deletion, DNA Cleavage, Genotyping Techniques
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