The ESX-3 Secretion System Is Necessary for Iron and Zinc Homeostasis in Mycobacterium tuberculosis
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ESX-3 is one of the five type VII secretion systems encoded by the Mycobacterium tuberculosis genome. We recently showed the essentiality of ESX-3 for M. tuberculosis viability and proposed its involvement in iron and zinc metabolism. In this study we confirmed the role of ESX-3 in iron uptake and its involvement in the adaptation to low zinc environment in M. tuberculosis. Moreover, we unveiled functional differences between the ESX-3 roles in M. tuberculosis and M. smegmatis showing that in the latter ESX-3 is only involved in the adaptation to iron and not to zinc restriction. Finally, we also showed that in M. tuberculosis this secretion system is essential for iron and zinc homeostasis not only in conditions in which the concentrations of these metals are limiting but also in metal sufficient conditions.
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ESX-3 is necessary for adaptation of M. tuberculosis to low Fe conditions.
“The precise role of ESX-3 in Fe acquisition in M. tuberculosis is unknown, but it is clear that ESX-3 is necessary for adaptation to low Fe conditions [62].”
From Strategies of intracellular pathogens for obtaining iron from the environment (2015) · cites this paper as [62]
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- Authors
- Serafini, A., Pisu, D., Palù, G., Rodriguez, G. M., & Manganelli, R.
- Affiliations
- University of Padua (IT)
- Corresponding author
- Manganelli, Riccardo
- Venue
- PLoS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2013-10-14
- Volume
- 8
- Issue
- 10
- Pages
- e78351
- ISSN
- 1932-6203 (ISSN-L)
- PMC
- PMC3796483
- OpenAlex
- W2088316319
- Cited by
- 131
- References
- 71
- Citations by year
- 2026: 12 · 2025: 13 · 2024: 6 · 2023: 8 · 2022: 11 · 2021: 10 · 2020: 10 · 2019: 13 · 2018: 12 · 2017: 11 · 2016: 11 · 2015: 9 · 2014: 5
- FWCI
- 6.08
- Type
- journal article
- Language
- en
- Update notices
- correction (2014-01-06)
- Open access
- gold
- doi.org
- Locations
- PLoS ONE (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, other-oa)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Figshare (submittedVersion, cc-by)
- Padua Research Archive (University of Padova) (submittedVersion, cc-by)
- Funders
- European Commission (201762)
- National Institutes of Health (FP7/2007-2013)
- National Institute of Allergy and Infectious Diseases (R01AI044856, R56AI044856, AI044856)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Tuberculosis Research and Epidemiology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Health Sciences › Medicine › Epidemiology › Mycobacterium research and diagnosis
- Keywords
- type VII secretion system, Mycobacterium tuberculosis, Mycobacterium smegmatis, iron homeostasis, zinc homeostasis, iron absorption, metal metabolism, bacterial virulence
- MeSH terms
- Bacterial Proteins, Cell Line, Cell Wall, Genes, Bacterial, Hemin, Homeostasis, Humans, Iron, Macrophages, Mutation, Mycobacterium tuberculosis, Oxazoles, Permeability, Streptonigrin, Transcription, Genetic, Zinc, Gene Expression Regulation, Bacterial, Mycobacterium smegmatis, Intracellular Space, Bacterial Secretion Systems
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