Cited by 131Tuberculosis Research and EpidemiologyBacterial Genetics and BiotechnologyMycobacterium research and diagnosis

The ESX-3 Secretion System Is Necessary for Iron and Zinc Homeostasis in Mycobacterium tuberculosis

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Serafini, Agnese, Pisu, Davide, Palù, Giorgio, Rodriguez, G. Marcela, Manganelli, Riccardo·2013·PLoS ONE

Abstract

ESX-3 is one of the five type VII secretion systems encoded by the Mycobacterium tuberculosis genome. We recently showed the essentiality of ESX-3 for M. tuberculosis viability and proposed its involvement in iron and zinc metabolism. In this study we confirmed the role of ESX-3 in iron uptake and its involvement in the adaptation to low zinc environment in M. tuberculosis. Moreover, we unveiled functional differences between the ESX-3 roles in M. tuberculosis and M. smegmatis showing that in the latter ESX-3 is only involved in the adaptation to iron and not to zinc restriction. Finally, we also showed that in M. tuberculosis this secretion system is essential for iron and zinc homeostasis not only in conditions in which the concentrations of these metals are limiting but also in metal sufficient conditions.

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Authors
Serafini, A., Pisu, D., Palù, G., Rodriguez, G. M., & Manganelli, R.
Affiliations
University of Padua (IT)
Corresponding author
Manganelli, Riccardo
Venue
PLoS ONE
Published
2013-10-14
Volume
8
Issue
10
Pages
e78351
ISSN
1932-6203 (ISSN-L)
OpenAlex
W2088316319
Cited by
131
References
71
Citations by year
2026: 12 · 2025: 13 · 2024: 6 · 2023: 8 · 2022: 11 · 2021: 10 · 2020: 10 · 2019: 13 · 2018: 12 · 2017: 11 · 2016: 11 · 2015: 9 · 2014: 5
FWCI
6.08
Type
journal article
Language
en
Open access
Locations
  • PLoS ONE (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, other-oa)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • Figshare (submittedVersion, cc-by)
  • Padua Research Archive (University of Padova) (submittedVersion, cc-by)
Funders
  • European Commission (201762)
  • National Institutes of Health (FP7/2007-2013)
  • National Institute of Allergy and Infectious Diseases (R01AI044856, R56AI044856, AI044856)
Topics
  • Health Sciences › Medicine › Infectious Diseases › Tuberculosis Research and Epidemiology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
  • Health Sciences › Medicine › Epidemiology › Mycobacterium research and diagnosis
Keywords
type VII secretion system, Mycobacterium tuberculosis, Mycobacterium smegmatis, iron homeostasis, zinc homeostasis, iron absorption, metal metabolism, bacterial virulence
MeSH terms
Bacterial Proteins, Cell Line, Cell Wall, Genes, Bacterial, Hemin, Homeostasis, Humans, Iron, Macrophages, Mutation, Mycobacterium tuberculosis, Oxazoles, Permeability, Streptonigrin, Transcription, Genetic, Zinc, Gene Expression Regulation, Bacterial, Mycobacterium smegmatis, Intracellular Space, Bacterial Secretion Systems

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