Cited by 198Tuberculosis Research and EpidemiologyBacterial Genetics and BiotechnologyMycobacterium research and diagnosis

Characterization of a Mycobacterium tuberculosis ESX-3 Conditional Mutant: Essentiality and Rescue by Iron and Zinc

Open access
Serafini, Agnese, Boldrin, Francesca, Palù, Giorgio, Manganelli, Riccardo·2009·Journal of Bacteriology

Abstract

Recently, a novel type of secretory pathway, type VII secretion systems (T7SSs), has been characterized in mycobacteria. The chromosomes of Mycobacterium tuberculosis and Mycobacterium bovis encode five T7SSs (ESX-1 to ESX-5). The best characterized of them, ESX-1, is involved in host-pathogen interactions, and its deletion is one of the main causes of M. bovis BCG attenuation. Another T7SS, ESX-3, has been previously shown to be transcriptionally controlled by the zinc uptake repressor (Zur) and by the iron-dependent transcriptional repressor (IdeR), suggesting that it might be involved in zinc and iron homeostasis. In this study, we characterized an M. tuberculosis conditional mutant in which transcription of the ESX-3 gene cluster can be downregulated by anhydrotetracycline. We showed that this T7SS is essential for growth and that this phenotype can be complemented by zinc, iron, or supernatant from a wild-type parental strain culture, demonstrating that the ESX-3 secretion system is responsible for the secretion of some soluble factor(s) required for growth that is probably involved in optimal iron and zinc uptake.

Integrity signals

Registry

Crossref · 10.1128/jb.00756-09

Cites

29 of 29 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

We couldn’t retrieve this paper’s full text, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Serafini, A., Boldrin, F., Palù, G., & Manganelli, R.
Affiliations
  • University of Padua (IT)
  • Department of Histology, Microbiology, and Medical Biotechnologies, University of Padua, Padua, Italy
Venue
Journal of Bacteriology
Published
2009-10-15
Volume
191
Issue
20
Pages
6340-6344
ISSN
  • 0021-9193 (ISSN-L)
  • 1098-5530
OpenAlex
W1974305305
Cited by
198
References
29
Citations by year
2026: 7 · 2025: 6 · 2024: 5 · 2023: 12 · 2022: 11 · 2021: 11 · 2020: 6 · 2019: 14 · 2018: 9 · 2017: 16 · 2016: 20 · 2015: 20 · 2014: 15 · 2013: 18 · 2012: 8
FWCI
6.41
Type
journal article
Language
en
Open access
Locations
  • Journal of Bacteriology (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • Padua Research Archive (University of Padova) (submittedVersion)
Topics
  • Health Sciences › Medicine › Infectious Diseases › Tuberculosis Research and Epidemiology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
  • Health Sciences › Medicine › Epidemiology › Mycobacterium research and diagnosis
Keywords
Mycobacterium tuberculosis, type VII secretion system, conditional mutants, anhydrotetracycline, zinc, iron, gene essentiality, soluble factors
MeSH terms
Bacterial Proteins, Iron, Mutation, Mycobacterium tuberculosis, Time Factors, Zinc, Culture Media, Conditioned

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review