Optogenetic Recruitment of Dorsal Raphe Serotonergic Neurons Acutely Decreases Mechanosensory Responsivity in Behaving Mice
Open accessAbstract
The inhibition of sensory responsivity is considered a core serotonin function, yet this hypothesis lacks direct support due to methodological obstacles. We adapted an optogenetic approach to induce acute, robust and specific firing of dorsal raphe serotonergic neurons. In vitro, the responsiveness of individual dorsal raphe serotonergic neurons to trains of light pulses varied with frequency and intensity as well as between cells, and the photostimulation protocol was therefore adjusted to maximize their overall output rate. In vivo, the photoactivation of dorsal raphe serotonergic neurons gave rise to a prominent light-evoked field response that displayed some sensitivity to a 5-HT1A agonist, consistent with autoreceptor inhibition of raphe neurons. In behaving mice, the photostimulation of dorsal raphe serotonergic neurons produced a rapid and reversible decrease in the animals' responses to plantar stimulation, providing a new level of evidence that serotonin gates sensory-driven responses.
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Increases in serotonin levels reduce behavioural sensitivity to mechanosensory stimuli.
“Nevertheless, consistent with the notion that serotonin reduces neural gain, increases in serotonin levels reduce behavioural sensitivity to mechanosensory stimuli202 and reduce startle responses203.”
From Mechanisms underlying gain modulation in the cortex (2020) · cites this paper as [202]
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- Authors
- Dugué, G. P., Lörincz, M. L., Lottem, E., Audero, E., Matias, S., Correia, P. A., Léna, C., & Mainen, Z. F.
- Affiliations
- Centre National de la Recherche Scientifique (FR)
- University of Szeged (HU)
- Corresponding author
- Dugué, Guillaume P.
- Mainen, Zachary F.
- ORCID
- Venue
- PLoS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2014-08-22
- Volume
- 9
- Issue
- 8
- Pages
- e105941
- ISSN
- 1932-6203 (ISSN-L)
- PMC
- PMC4141837
- OpenAlex
- W2037721317
- Cited by
- 54
- References
- 41
- Citations by year
- 2025: 2 · 2024: 2 · 2023: 4 · 2022: 3 · 2021: 2 · 2020: 8 · 2019: 7 · 2018: 4 · 2017: 8 · 2016: 6 · 2015: 5 · 2014: 2
- FWCI
- 3.20
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- PLoS ONE (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT) (submittedVersion, other-oa)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Figshare (submittedVersion, cc-by)
- Funders
- European Commission (220098)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurotransmitter Receptor Influence on Behavior
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Photoreceptor and optogenetics research
- Keywords
- dorsal raphe nucleus, serotonergic neurons, optogenetic stimulation, sensory gating, plantar stimulation
- MeSH terms
- Animals, Behavior, Animal, Mice, Transgenic, Neurons, Organ Culture Techniques, Photic Stimulation, Serotonin, 8-Hydroxy-2-(di-n-propylamino)tetralin, Mechanotransduction, Cellular, Serotonin 5-HT1 Receptor Agonists, Optogenetics, Dorsal Raphe Nucleus
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