CoChat
Sign Up
Cited by 323Bone Metabolism and DiseasesBone health and osteoporosis researchOsteomyelitis and Bone Disorders Research

The DOI matches its Crossref record; 38 of the 38 references it cites were found in a registry or an index.

Osteocytes, not Osteoblasts or Lining Cells, are the Main Source of the RANKL Required for Osteoclast Formation in Remodeling Bone

Open access
Xiong, Jinhu, Piemontese, Marilina, Onal, Melda, Campbell, Josh, Goellner, Joseph J., Dusevich, Vladimir, Bonewald, Lynda, Manolagas, Stavros C., O’Brien, Charles A.·2015·PLOS ONE
DOI 10.1371/journal.pone.0138189PMC PMC4578942
Open this paper in CoChat — free accountOpen PDF

Abstract

The cytokine receptor activator of nuclear factor kappa B ligand (RANKL), encoded by the Tnfsf11 gene, is essential for osteoclastogenesis and previous studies have shown that deletion of the Tnfsf11 gene using a Dmp1-Cre transgene reduces osteoclast formation in cancellous bone by more than 70%. However, the Dmp1-Cre transgene used in those studies leads to recombination in osteocytes, osteoblasts, and lining cells making it unclear whether one or more of these cell types produce the RANKL required for osteoclast formation in cancellous bone. Because osteoblasts, osteocytes, and lining cells have distinct locations and functions, distinguishing which of these cell types are sources of RANKL is essential for understanding the orchestration of bone remodeling. To distinguish between these possibilities, we have now created transgenic mice expressing the Cre recombinase under the control of regulatory elements of the Sost gene, which is expressed in osteocytes but not osteoblasts or lining cells in murine bone. Activity of the Sost-Cre transgene in osteocytes, but not osteoblast or lining cells, was confirmed by crossing Sost-Cre transgenic mice with tdTomato and R26R Cre-reporter mice, which express tdTomato fluorescent protein or LacZ, respectively, only in cells expressing the Cre recombinase or their descendants. Deletion of the Tnfsf11 gene in Sost-Cre mice led to a threefold decrease in osteoclast number in cancellous bone and increased cancellous bone mass, mimicking the skeletal phenotype of mice in which the Tnfsf11 gene was deleted using the Dmp1-Cre transgene. These results demonstrate that osteocytes, not osteoblasts or lining cells, are the main source of the RANKL required for osteoclast formation in remodeling cancellous bone.

Overview

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1371/journal.pone.0138189

Cites

38 of 38 references verified0 citation errors · 0 not checkable

Statistics

Figures

Fig 1 Generation of Sost-Cre transgenic mice. (A) Diagram of a portion of the Sost-Cre transgene construct used to generate Sost-Cre transgenic mice. UTR, untranslated region, IRES, internal ribosome entry site, GFP::Cre, fusion protein of GFP and the Cre recombinase. (B) Sost-Cre transgene mRNA levels in different tissues of 6-week-old mice male mice as measured by quantitative RT-PCR. n = 4 animals for each tissue.
Fig 1 Generation of Sost-Cre transgenic mice. (A) Diagram of a portion of the Sost-Cre transgene construct used to generate Sost-Cre transgenic mice. UTR, untranslated region, IRES, internal ribosome entry site, GFP::Cre, fusion protein of GFP and the Cre recombinase. (B) Sost-Cre transgene mRNA levels in different tissues of 6-week-old mice male mice as measured by quantitative RT-PCR. n = 4 animals for each tissue.CC0 · PubMed Central
Fig 2 Anaylsis of Sost-Cre transgenic mice using tdTomato Cre-reporter mice. Fluorescent images of frozen histological sections of femurs from 2-month-old tdTomato control, Sost-Cre;tdTomato, and 10 kb Dmp1-Cre;tdTomato mice injected with calcein. (A) Confocal image of osteocytes in cortical bone. (B) Epifluorescent image of endocortical surface. (C) Epifluorescent image of cancellous bone. (D) Higher magnification of the cancellous bone from (C). Scale bar, 50 μm. Green: calcein labeling of mineralizing bone; Red: cells that express tdTomato fluorescent protein upon Cre-mediated recombination.
Fig 2 Anaylsis of Sost-Cre transgenic mice using tdTomato Cre-reporter mice. Fluorescent images of frozen histological sections of femurs from 2-month-old tdTomato control, Sost-Cre;tdTomato, and 10 kb Dmp1-Cre;tdTomato mice injected with calcein. (A) Confocal image of osteocytes in cortical bone. (B) Epifluorescent image of endocortical surface. (C) Epifluorescent image of cancellous bone. (D) Higher magnification of the cancellous bone from (C). Scale bar, 50 μm. Green: calcein labeling of mineralizing bone; Red: cells that express tdTomato fluorescent protein upon Cre-mediated recombination.CC0 · PubMed Central
Fig 3 Analysis of Sost-Cre transgenic mice using R26R Cre-reporter mice. (A) Brightfield microscopy images of X-gal stained frozen histological sections of cortical bone and (B) cancellous bone in the femur of 2-month-old R26R, Sost-Cre;R26R, and 10 kb Dmp1-Cre;R26R mice. (C) Epifluorescent image of the same cancellous bone as in (B) showing calcein labeling. Scale bar, 50 μm.
Fig 3 Analysis of Sost-Cre transgenic mice using R26R Cre-reporter mice. (A) Brightfield microscopy images of X-gal stained frozen histological sections of cortical bone and (B) cancellous bone in the femur of 2-month-old R26R, Sost-Cre;R26R, and 10 kb Dmp1-Cre;R26R mice. (C) Epifluorescent image of the same cancellous bone as in (B) showing calcein labeling. Scale bar, 50 μm.CC0 · PubMed Central
Fig 4 Analysis of Sost-Cre transgene activity by transmission electron microscopy. Transmission electron microscopy (TEM) images of electron-dense X-gal deposits in osteocytes (A), osteoblasts (B), and lining cells (C) on the cancellous bone surface of vertebra of 2-month-old R26R, Sost-Cre;R26R, and 10 kb Dmp1-Cre;R26R mice. Size bars are present in each image, with the size indicated.
Fig 4 Analysis of Sost-Cre transgene activity by transmission electron microscopy. Transmission electron microscopy (TEM) images of electron-dense X-gal deposits in osteocytes (A), osteoblasts (B), and lining cells (C) on the cancellous bone surface of vertebra of 2-month-old R26R, Sost-Cre;R26R, and 10 kb Dmp1-Cre;R26R mice. Size bars are present in each image, with the size indicated.CC0 · PubMed Central
Fig 5 Deletion of Tnfsf11 using the Sost-Cre transgene increases bone mass. (A) Quantitative PCR of loxP-flanked Tnfsf11 genomic DNA using genomic DNA isolated from collagenase-digested femoral cortical bone of 6-month-old Sost-Cre;Tnfsf11-f/f (n = 10) and Tnfsf11-f/f (n = 16) littermates. *P < 0.05 using Student’s t-test. Representative images of the teeth (B) and safranin-O-stained histological sections of the lumbar vertebra (Scale bar, 0.1 mm) (C) of 6-month-old Sost-Cre;Tnfsf11-f/f and Tnfsf11-f/f mice. (D) Serial femoral and spinal BMD of Sost-Cre;Tnfsf11-f/f (n = 14), Tnfsf11-f/f (n = 16), wild-type (n = 14), and Sost-Cre (n = 13) littermates measured by DXA. *P < 0.05 versus wild-type, Tnfsf11-f/f, or Sost-Cre mice using one-way ANOVA comparing the 4 genotypes at a given age. All results include data from both male and female mice.
Fig 5 Deletion of Tnfsf11 using the Sost-Cre transgene increases bone mass. (A) Quantitative PCR of loxP-flanked Tnfsf11 genomic DNA using genomic DNA isolated from collagenase-digested femoral cortical bone of 6-month-old Sost-Cre;Tnfsf11-f/f (n = 10) and Tnfsf11-f/f (n = 16) littermates. *P < 0.05 using Student’s t-test. Representative images of the teeth (B) and safranin-O-stained histological sections of the lumbar vertebra (Scale bar, 0.1 mm) (C) of 6-month-old Sost-Cre;Tnfsf11-f/f and Tnfsf11-f/f mice. (D) Serial femoral and spinal BMD of Sost-Cre;Tnfsf11-f/f (n = 14), Tnfsf11-f/f (n = 16), wild-type (n = 14), and Sost-Cre (n = 13) littermates measured by DXA. *P < 0.05 versus wild-type, Tnfsf11-f/f, or Sost-Cre mice using one-way ANOVA comparing the 4 genotypes at a given age. All results include data from both male and female mice.CC0 · PubMed Central
Fig 6 Cancellous bone volume is high in Sost-Cre;Tnfsf11-f/f mice. (A) Cancellous bone volume over total volume (BV/TV), trabecular number (Tb.N), trabecular thickness (Tb.Th), and trabecular separation (Tb.Sp) in the L4 vertebra of 6-month-old Sost-Cre;Tnfsf11-f/f (n = 10) and Tnfsf11-f/f (n = 16) littermates. *P < 0.05 using Student’s t-test. (B) Cancellous BV/TV, trabecular number, trabecular thickness, trabecular separation, and cortical thickness (Ct.Th) in the distal femur of the same mice as in (A). *P < 0.05 using Student’s t-test. Representative μCT images of the L4 vertebra (C) and distal femur (D) of 6-month-old Sost-Cre;Tnfsf11-f/f and Tnfsf11-f/f littermates. All results include data from both male and female mice. Scale bar, 0.5 mm.
Fig 6 Cancellous bone volume is high in Sost-Cre;Tnfsf11-f/f mice. (A) Cancellous bone volume over total volume (BV/TV), trabecular number (Tb.N), trabecular thickness (Tb.Th), and trabecular separation (Tb.Sp) in the L4 vertebra of 6-month-old Sost-Cre;Tnfsf11-f/f (n = 10) and Tnfsf11-f/f (n = 16) littermates. *P < 0.05 using Student’s t-test. (B) Cancellous BV/TV, trabecular number, trabecular thickness, trabecular separation, and cortical thickness (Ct.Th) in the distal femur of the same mice as in (A). *P < 0.05 using Student’s t-test. Representative μCT images of the L4 vertebra (C) and distal femur (D) of 6-month-old Sost-Cre;Tnfsf11-f/f and Tnfsf11-f/f littermates. All results include data from both male and female mice. Scale bar, 0.5 mm.CC0 · PubMed Central
Fig 7 Osteoclastogenesis is inhibited in Sost-Cre;Tnfsf11-f/f mice. (A) Quantitative RT-PCR for Tnfsf11 mRNA in tibia, L5 vertebra, and calvaria of 6-month-old Sost-Cre;Tnfsf11-f/f (n = 10) and Tnfsf11-f/f (n = 16) littermates. *P < 0.05 using Student’s t-test. (B-C) The perimeter of bone surface covered by osteoclasts (Oc.Pm/B.Pm) (B) and the osteoclast number per mm of bone surface (N.Oc/B.Pm) (C) in cancellous bone of the vertebra (left panel) and distal femur (middle) and endocortical bone (right) of 6-month-old Sost-Cre;Tnfsf11-f/f (n = 5) and Tnfsf11-f/f (n = 5) littermates. *P < 0.05 using Student’s t-test. (D) Representative images of osteoclasts in cancellous bone (upper panel) and endocortical surface (lower panel) of 6-month-old Sost-Cre;Tnfsf11-f/f and Tnfsf11-f/f mice. All results include data from both male and female mice.
Fig 7 Osteoclastogenesis is inhibited in Sost-Cre;Tnfsf11-f/f mice. (A) Quantitative RT-PCR for Tnfsf11 mRNA in tibia, L5 vertebra, and calvaria of 6-month-old Sost-Cre;Tnfsf11-f/f (n = 10) and Tnfsf11-f/f (n = 16) littermates. *P < 0.05 using Student’s t-test. (B-C) The perimeter of bone surface covered by osteoclasts (Oc.Pm/B.Pm) (B) and the osteoclast number per mm of bone surface (N.Oc/B.Pm) (C) in cancellous bone of the vertebra (left panel) and distal femur (middle) and endocortical bone (right) of 6-month-old Sost-Cre;Tnfsf11-f/f (n = 5) and Tnfsf11-f/f (n = 5) littermates. *P < 0.05 using Student’s t-test. (D) Representative images of osteoclasts in cancellous bone (upper panel) and endocortical surface (lower panel) of 6-month-old Sost-Cre;Tnfsf11-f/f and Tnfsf11-f/f mice. All results include data from both male and female mice.CC0 · PubMed Central

Verification

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Citations

Cites

Show all 37 references

Cited by

Sorted by citations

Related

See all

Metadata

Authors
Xiong, J., Piemontese, M., Onal, M., Campbell, J., Goellner, J. J., Dusevich, V., Bonewald, L., Manolagas, S. C., & O’Brien, C. A.
Affiliations
  • Central Arkansas Veterans Healthcare System (US)
  • University of Missouri–Kansas City (US)
Corresponding author
O’Brien, Charles A.
ORCID
Venue
PLOS ONE
Publisher
Public Library of Science (PLoS)
Published
2015-09-22
Volume
10
Issue
9
Pages
e0138189
ISSN
1932-6203 (ISSN-L)
DOI
10.1371/journal.pone.0138189
PMC
PMC4578942
OpenAlex
W2195625763
Cited by
323
References
37
Citations by year
2026: 20 · 2025: 19 · 2024: 28 · 2023: 44 · 2022: 29 · 2021: 28 · 2020: 40 · 2019: 35 · 2018: 26 · 2017: 31 · 2016: 21 · 2015: 1
FWCI
11.42
Type
journal article
Language
en
Open access
Licence
Locations
  • PLoS ONE (publishedVersion, public-domain)
  • Europe PMC (PubMed Central) (publishedVersion, public-domain)
  • PubMed Central (publishedVersion, public-domain)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • Figshare (submittedVersion, cc-by)
Funders
  • U.S. Department of Veterans Affairs (I01 BX000294)
  • National Institutes of Health (RR029884, AG13918)
  • Translational Research Institute, University of Arkansas for Medical Sciences (RR029884, UL1 RR029884)
  • National Institute on Aging (AG13918, P01AG013918)
  • National Institute of Arthritis and Musculoskeletal and Skin Diseases (AR049794, R01AR049794)
  • National Center for Research Resources (UL1RR029884)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
  • Health Sciences › Medicine › Orthopedics and Sports Medicine › Bone health and osteoporosis research
  • Health Sciences › Medicine › Rheumatology › Osteomyelitis and Bone Disorders Research
Keywords
RANKL, osteocytes, osteoclastogenesis, bone remodeling, osteoblasts, bone lining cells, trabecular bone, bone mass
MeSH terms
Animals, Cell Division, Mice, Transgenic, Osteoblasts, Osteoclasts, Osteocytes, Recombination, Genetic, Bone Density, Bone Remodeling, Mice, RANK Ligand

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review