Aging Impairs the Ability of Conventional Dendritic Cells to Cross-Prime CD8+ T Cells upon Stimulation with a TLR7 Ligand
Open accessAbstract
The aging process is accompanied by altered immune system functioning and an increased risk of infection. Dendritic cells (DCs) are antigen-presenting cells that play a key role in both adaptive and innate immunity, but how aging affects DCs and their influence on immunity has not been thoroughly established. Here we examined the function of conventional DCs (cDCs) in old mice after TLR7 stimulation, focusing on their ability to cross-prime CD8+ T cells. Using polyU, a synthetic ssRNA analog, as TLR7 ligand and OVA as an antigen (Ag) model, we found that cDCs from old mice have a poor ability to stimulate a CD8+ T cell-mediated cytotoxic response. cDCs from old mice exhibit alterations in Ag-processing machinery and TLR7 activation. Remarkably, CD8α+ cDCs from old mice have an impaired ability to activate naïve CD8+ T cells and, moreover, a lower capacity to mature and to process exogenous Ag. Taken together, our results suggest that immunosenescence impacts cDC function, affecting the activation of naïve CD8+ T cells and the generation of effector cytotoxic T cells.
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From SARS-CoV-2, COVID-19 and the aging immune system (2021) · cites this paper as [25]
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- Authors
- Zacca, E. R., Crespo, M. I., Acland, R. P., Roselli, E., Núñez, N. G., Maccioni, M., Maletto, B. A., Pistoresi-Palencia, M. C., & Morón, G.
- Affiliations
- Consejo Nacional de Investigaciones Científicas y Técnicas (AR)
- Corresponding author
- Morón, Gabriel
- ORCID
- Venue
- PLOS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2015-10-16
- Volume
- 10
- Issue
- 10
- Pages
- e0140672
- ISSN
- 1932-6203 (ISSN-L)
- PMC
- PMC4608578
- OpenAlex
- W2339169121
- Cited by
- 66
- References
- 61
- Citations by year
- 2026: 6 · 2025: 6 · 2024: 3 · 2023: 4 · 2022: 12 · 2021: 18 · 2020: 6 · 2019: 2 · 2018: 3 · 2017: 5 · 2016: 1
- FWCI
- 1.48
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- PLoS ONE (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (publishedVersion, other-oa)
- Americanae (AECID Library) (submittedVersion, cc-by-nc-sa)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- Figshare (submittedVersion, cc-by)
- Greater South Information System
- Funders
- Fundación Florencio Fiorini
- Universidad Nacional de Córdoba
- Consejo Nacional de Investigaciones Científicas y Técnicas (PICT 2012)
- Agencia Nacional de Promoción Científica y Tecnológica (PICT-2012, PICT-2012-0591, CONICET)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Immunotherapy and Immune Responses
- Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
- Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
- Keywords
- immunosenescence, myeloid dendritic cells, TLR7 ligand, CD8α+ dendritic cells, dendritic cell maturation, antigen processing, cytotoxic T lymphocytes, aging, T cell activation
- MeSH terms
- Aging, Animals, Antigens, Dendritic Cells, Female, Immunity, Cellular, Membrane Glycoproteins, Poly U, Antigen Presentation, CD8-Positive T-Lymphocytes, Cross-Priming, Toll-Like Receptor 7, Mice
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