Cited by 111Gut microbiota and healthGastrointestinal motility and disordersAdipokines, Inflammation, and Metabolic Diseases

Gut microbiota varies by opioid use, circulating leptin and oxytocin in African American men with diabetes and high burden of chronic disease

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Barengolts, Elena, Green, Stefan J., Eisenberg, Yuval, Akbar, Arfana, Reddivari, Bharathi, Layden, Brian T., Dugas, Lara, Chlipala, George·2018·PLoS ONE

Abstract

OBJECTIVE: The gut microbiota is known to be related to type 2 diabetes (T2D), psychiatric conditions, and opioid use. In this study, we tested the hypothesis that variability in gut microbiota in T2D is associated with psycho-metabolic health. METHODS: A cross-sectional study was conducted among African American men (AAM) (n = 99) that were outpatients at a Chicago VA Medical Center. The main outcome measures included fecal microbiota ecology (by 16S rRNA gene sequencing), psychiatric disorders including opioid use, and circulating leptin and oxytocin as representative hormone biomarkers for obesity and psychological pro-social behavior. RESULTS: The study subjects had prevalent overweight/obesity (78%), T2D (50%) and co-morbid psychiatric (65%) and opioid use (45%) disorders. In the analysis of microbiota, the data showed interactions of opioids, T2D and metformin with Bifidobacterium and Prevotella genera. The differential analysis of Bifidobacterium stratified by opioids, T2D and metformin, showed significant interactions among these factors indicating that the effect of one factor was changed by the other (FDR-adjusted p [q] < 0.01). In addition, the pair-wise comparison showed that participants with T2D not taking metformin had a significant 6.74 log2 fold increase in Bifidobacterium in opioid users as compared to non-users (q = 2.2 x 10-8). Since metformin was not included in this pair-wise comparison, the significant 'q' suggested association of opioid use with Bifidobacterium abundance. The differences in Bifidobacterium abundance could possibly be explained by opioids acting as organic cation transporter 1 (OCT1) inhibitors. Analysis stratified by lower and higher leptin and oxytocin (divided by the 50th percentile) in the subgroup without T2D showed lower Dialister in High-Leptin vs. Low-Leptin (p = 0.03). Contrary, the opposite was shown for oxytocin, higher Dialister in High-Oxytocin vs. Low-Oxytocin (p = 0.04). CONCLUSIONS: The study demonstrated for the first time that Bifidobacterium and Prevotella abundance was affected by interactions of T2D, metformin and opioid use. Also, in subjects without T2D Dialister abundance varied according to circulating leptin and oxytocin.

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  • Laxatives and other over-the-counter remedies do not address underlying causes of opioid-induced bowel dysfunction, which may include microbial alterations.

    “While laxatives and other over-the-counter remedies can be used to treat opioid-induced bowel dysfunction, they do not address underlying causes which may include microbial alterations (173).”

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Authors
Barengolts, E., Green, S. J., Eisenberg, Y., Akbar, A., Reddivari, B., Layden, B. T., Dugas, L., & Chlipala, G.
Affiliations
  • University of Illinois Chicago (US)
  • Loyola University Chicago (US)
Corresponding author
Barengolts, Elena
Venue
PLoS ONE
Published
2018-03-29
Volume
13
Issue
3
Pages
e0194171
ISSN
1932-6203 (ISSN-L)
OpenAlex
W2795111952
Cited by
111
References
52
Citations by year
2026: 5 · 2025: 16 · 2024: 14 · 2023: 13 · 2022: 22 · 2021: 19 · 2020: 10 · 2019: 12
FWCI
3.65
Type
journal article
Language
en
Open access
Locations
  • PLoS ONE (publishedVersion, public-domain)
  • Europe PMC (PubMed Central) (publishedVersion, public-domain)
  • PubMed Central (publishedVersion, public-domain)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • Westside Institute for Science and Education
  • National Institutes of Health (R01DK104927-01A1)
  • U.S. Department of Veterans Affairs (1IK2BX001587-01)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
  • Health Sciences › Medicine › Gastroenterology › Gastrointestinal motility and disorders
  • Health Sciences › Medicine › Epidemiology › Adipokines, Inflammation, and Metabolic Diseases
Keywords
gut microbiota, type 2 diabetes, opioid use, metformin, Bifidobacterium, Prevotella, leptin, oxytocin, Dialister, psychiatric comorbidity, obesity, African American men, OCT1, chronic disease burden, veterans, 16S rRNA gene sequencing
MeSH terms
Gastrointestinal Microbiome, Bacteria, Black or African American, Chronic Disease, Cross-Sectional Studies, Diabetes Mellitus, Type 2, Humans, Male, Metformin, Opioid-Related Disorders, Oxytocin, Cost of Illness, Leptin

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