Cited by 266Gut microbiota and healthGastrointestinal motility and disordersOpioid Use Disorder Treatment

Opioid-induced gut microbial disruption and bile dysregulation leads to gut barrier compromise and sustained systemic inflammation

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Banerjee, S., Sindberg, G., Wang, F., Meng, J., Sharma, U., Zhang, L., Dauer, P., Chen, C. +3 more·2016·Mucosal Immunology

Abstract

Morphine and its pharmacological derivatives are the most prescribed analgesics for moderate to severe pain management. However, chronic use of morphine reduces pathogen clearance and induces bacterial translocation across the gut barrier. The enteric microbiome has been shown to have a critical role in the preservation of the mucosal barrier function and metabolic homeostasis. Here, we show for the first time, using bacterial 16s rDNA sequencing, that chronic morphine treatment significantly alters the gut microbial composition and induces preferential expansion of Gram-positive pathogenic and reduction in bile-deconjugating bacterial strains. A significant reduction in both primary and secondary bile acid levels was seen in the gut, but not in the liver with morphine treatment. Morphine-induced microbial dysbiosis and gut barrier disruption was rescued by transplanting placebo-treated microbiota into morphine-treated animals, indicating that microbiome modulation could be exploited as a therapeutic strategy for patients using morphine for pain management.

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  1. [50]Weingarden AR Microbiota transplantation restores normal fecal bile acid composition in recurrent Clostridium difficile infection Am J Physiol Gastrointest Liver Physiol 306 G310 9 2014 24284963 PMC3920123

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Authors
Banerjee, S., Sindberg, G., Wang, F., Meng, J., Sharma, U., Zhang, L., Dauer, P., Chen, C., Dalluge, J., Johnson, T., & Roy, S.
Affiliations
University of Minnesota (US)
Corresponding author
Roy, S.
Venue
Mucosal Immunology
Publisher
Elsevier BV
Published
2016-11
Volume
9
Issue
6
Pages
1418-1428
ISSN
1933-0219 (ISSN-L)
OpenAlex
W2289575399
Cited by
266
References
52
Citations by year
2026: 19 · 2025: 27 · 2024: 34 · 2023: 39 · 2022: 41 · 2021: 26 · 2020: 28 · 2019: 21 · 2018: 19 · 2017: 7 · 2016: 5
FWCI
9.01
Type
journal article
Language
en
Open access
Locations
  • Mucosal Immunology (publishedVersion)
  • PubMed (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • University of Minnesota
  • National Institutes of Health
  • National Heart, Lung, and Blood Institute (R21HL125021)
  • National Institute on Drug Abuse (K05DA033881, P50DA011806, R01DA022935, R01DA031202, R01DA037843, T32DA007097, R01DA034582, R01DA012104)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
  • Health Sciences › Medicine › Gastroenterology › Gastrointestinal motility and disorders
  • Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Opioid Use Disorder Treatment
Keywords
morphine, opioid analgesics, gut dysbiosis, intestinal barrier dysfunction, bacterial translocation, systemic inflammation, Gram-positive bacteria, microbiota transplantation, chronic opioid use
MeSH terms
Gastrointestinal Microbiome, Fecal Microbiota Transplantation, Analgesics, Opioid, Animals, Bile, Bile Acids and Salts, Cholesterol, Immune System, Inflammation, Intestinal Mucosa, Liver, Morphine, Receptors, Opioid, mu, Mice, Knockout, Immunity, Mucosal, Toll-Like Receptor 2, Mice, Dysbiosis

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