Opioid-induced gut microbial disruption and bile dysregulation leads to gut barrier compromise and sustained systemic inflammation
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Morphine and its pharmacological derivatives are the most prescribed analgesics for moderate to severe pain management. However, chronic use of morphine reduces pathogen clearance and induces bacterial translocation across the gut barrier. The enteric microbiome has been shown to have a critical role in the preservation of the mucosal barrier function and metabolic homeostasis. Here, we show for the first time, using bacterial 16s rDNA sequencing, that chronic morphine treatment significantly alters the gut microbial composition and induces preferential expansion of Gram-positive pathogenic and reduction in bile-deconjugating bacterial strains. A significant reduction in both primary and secondary bile acid levels was seen in the gut, but not in the liver with morphine treatment. Morphine-induced microbial dysbiosis and gut barrier disruption was rescued by transplanting placebo-treated microbiota into morphine-treated animals, indicating that microbiome modulation could be exploited as a therapeutic strategy for patients using morphine for pain management.
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Most of the opioid research has been conducted during methadone maintenance, due to the difficulties conducting research on individuals using illicit drugs.
“Additionally, most of the opioid research has been conducted during methadone maintenance (162, 168–170), due to the difficulties conducting research on individuals using illicit drugs.”
From A Biopsychosocial Overview of the Opioid Crisis: Considering Nutrition and Gastrointestinal Health (2019) · cites this paper as [162]
Vitamin and mineral deficiencies associated with opioid addiction have been well-described.
“Vitamin and mineral deficiencies associated with opioid addiction have been well-described (160–165).”
From A Biopsychosocial Overview of the Opioid Crisis: Considering Nutrition and Gastrointestinal Health (2019) · cites this paper as [162]
Animal models that have opioid-induced gut microbial disruption, altered cholesterol/bile acid metabolism and systemic inflammation can be "rescued" by microbiota fecal transplantation positively influencing gut health.
“Animal models that have opioid-induced gut microbial disruption, altered cholesterol/bile acid metabolism and systemic inflammation can be “rescued” by microbiota fecal transplantation positively influencing gut health (162).”
From A Biopsychosocial Overview of the Opioid Crisis: Considering Nutrition and Gastrointestinal Health (2019) · cites this paper as [162]
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- Authors
- Banerjee, S., Sindberg, G., Wang, F., Meng, J., Sharma, U., Zhang, L., Dauer, P., Chen, C., Dalluge, J., Johnson, T., & Roy, S.
- Affiliations
- University of Minnesota (US)
- Corresponding author
- Roy, S.
- ORCID
- Venue
- Mucosal Immunology
- Publisher
- Elsevier BV
- Published
- 2016-11
- Volume
- 9
- Issue
- 6
- Pages
- 1418-1428
- ISSN
- 1933-0219 (ISSN-L)
- PMC
- PMC4996771
- OpenAlex
- W2289575399
- Cited by
- 266
- References
- 52
- Citations by year
- 2026: 19 · 2025: 27 · 2024: 34 · 2023: 39 · 2022: 41 · 2021: 26 · 2020: 28 · 2019: 21 · 2018: 19 · 2017: 7 · 2016: 5
- FWCI
- 9.01
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Mucosal Immunology (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- University of Minnesota
- National Institutes of Health
- National Heart, Lung, and Blood Institute (R21HL125021)
- National Institute on Drug Abuse (K05DA033881, P50DA011806, R01DA022935, R01DA031202, R01DA037843, T32DA007097, R01DA034582, R01DA012104)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Health Sciences › Medicine › Gastroenterology › Gastrointestinal motility and disorders
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Opioid Use Disorder Treatment
- Keywords
- morphine, opioid analgesics, gut dysbiosis, intestinal barrier dysfunction, bacterial translocation, systemic inflammation, Gram-positive bacteria, microbiota transplantation, chronic opioid use
- MeSH terms
- Gastrointestinal Microbiome, Fecal Microbiota Transplantation, Analgesics, Opioid, Animals, Bile, Bile Acids and Salts, Cholesterol, Immune System, Inflammation, Intestinal Mucosa, Liver, Morphine, Receptors, Opioid, mu, Mice, Knockout, Immunity, Mucosal, Toll-Like Receptor 2, Mice, Dysbiosis
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