Regulation of angiopoietin-like protein 4 production during and after exercise
Open accessAbstract
Angiopoietin-like protein 4 (ANGPTL4) may regulate lipoprotein lipase-dependent plasma clearance of triacylglycerol from skeletal muscle during exercise. The aim of this study was to examine the importance of muscle in regulating ANGPTL4 in response to exercise. We sampled muscle biopsies and serum before, immediately after, and 2 h after 45 min of ergometer cycling. Sampling was done before and after a 12-week training intervention in controls and dysglycemic subjects. Moreover, fat biopsies were taken before and after the training intervention. The regulation of ANGPTL4 was also investigated in several tissues of exercising mice, and in cultured myotubes. ANGPTL4 levels in serum and expression in muscle were highest 2 h after exercise in both groups. Whereas ANGPTL4 was higher in muscle of exercising controls as compared to dysglycemic subjects, the opposite was observed in serum. In exercising mice, Angptl4 mRNA showed both higher basal expression and induction in liver compared to muscle. Angptl4 mRNA was much higher in adipose tissue than muscle and was also induced by exercise. We observed two mRNA isoforms of ANGPTL4 in muscle and fat in humans. Both were induced by exercise in muscle; one isoform was expressed 5- to 10-fold higher than the other. Studies in mice and cultured myotubes showed that both fatty acids and cortisol have the potential to increase ANGPTL4 expression in muscle during exercise. In conclusion, ANGPTL4 is markedly induced in muscle in response to exercise. However, liver and adipose tissue may contribute more than muscle to the exercise-induced increase in circulating ANGPTL4.
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ANGPTL4 is upregulated by SkM after aerobic or resistance exercise.
“Although it is upregulated by SkM after aerobic or resistance exercise [171,172,196], surprisingly, an even greater degree of ANGPTL4 mRNA expression was found in the portion of SkM that remained inactive during the bout of exercise compared with the active SkM [172,196], representing a mechanism to redistribute FFA delivery from unexercised to exercised SkM.”
From The Role of Exercise in the Interplay between Myokines, Hepatokines, Osteokines, Adipokines, and Modulation of Inflammation for Energy Substrate Redistribution and Fat Mass Loss: A Review (2020) · cites this paper as [171]
The Results report increased skeletal-muscle ANGPTL4 mRNA after an acute cycling challenge, supporting the aerobic-exercise part. The Methods describe a 12-week program combining strength and endurance training, but the measured acute response was to cycling; the paper does not establish that resistance exercise alone upregulates skeletal-muscle ANGPTL4. Page numbers are unavailable in our copy. (LLM, conf=0.86)
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- Authors
- Norheim, F., Hjorth, M., Langleite, T. M., Lee, S., Holen, T., Bindesbøll, C., Stadheim, H. K., Gulseth, H. L., Birkeland, K. I., Kielland, A., Jensen, J., Dalen, K. T., & Drevon, C. A.
- Affiliations
- University of Oslo (NO)
- Department of Nutrition; Faculty of Medicine; Institute of Basic Medical Sciences; University of Oslo; Oslo Norway
- Norwegian School of Sport Sciences (NO)
- Oslo University Hospital (NO)
- Corresponding author
- Norheim, Frode
- ORCID
- Venue
- Physiological Reports
- Publisher
- Wiley
- Published
- 2014-08
- Volume
- 2
- Issue
- 8
- Pages
- e12109
- ISSN
- 2051-817X (ISSN-L)
- PMC
- PMC4246580
- OpenAlex
- W2112003863
- Cited by
- 53
- References
- 34
- Citations by year
- 2024: 13 · 2023: 2 · 2022: 7 · 2020: 6 · 2019: 5 · 2018: 9 · 2017: 3 · 2016: 3 · 2015: 5
- FWCI
- 2.52
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Physiological Reports (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- BIBSYS Brage (BIBSYS (Norway)) (submittedVersion, cc-by)
- NORA - Norwegian Open Research Archives (submittedVersion, other-oa)
- Funders
- Institute of Basic Medical Sciences
- UiO
- Helse Sør-Øst
- Johan Throne-Holst Foundation for Nutrition Research
- Freia Medical Research Foundation
- EU-financed FP7 project
- NutriTech grant (289511)
- Topics
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Lipid metabolism and disorders
- Health Sciences › Medicine › Complementary and alternative medicine › Cardiovascular and exercise physiology
- Health Sciences › Medicine › Rehabilitation › Exercise and Physiological Responses
- Keywords
- ANGPTL4, physical activity, skeletal muscle, lipoprotein lipase, dysglycemia, exercise training, mRNA isoforms, adipose tissue, liver, cortisol, fatty acids, myotubes
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