Cited by 83Muscle Physiology and DisordersExercise and Physiological ResponsesAdipokines, Inflammation, and Metabolic Diseases

Myostatin in relation to physical activity and dysglycaemia and its effect on energy metabolism in human skeletal muscle cells

Hjorth, M., Pourteymour, S., Görgens, S. W., Langleite, T. M., Lee, S., Holen, T., Gulseth, H. L., Birkeland, K. I. +3 more·2016·Acta Physiologica

Abstract

AIM: Some health benefits of exercise may be explained by an altered secretion of myokines. Because previous focus has been on upregulated myokines, we screened for downregulated myokines and identified myostatin. We studied the expression of myostatin in relation to exercise and dysglycaemia in skeletal muscle, adipose tissue and plasma. We further examined some effects of myostatin on energy metabolism in primary human muscle cells and Simpson-Golabi-Behmel syndrome (SGBS) adipocytes. METHODS: Sedentary men with or without dysglycaemia underwent a 45-min acute bicycle test before and after 12 weeks of combined endurance and strength training. Blood samples and biopsies from m. vastus lateralis and adipose tissue were collected. RESULTS: Myostatin mRNA expression was reduced in skeletal muscle after acute as well as long-term exercise and was even further downregulated by acute exercise on top of 12-week training. Furthermore, the expression of myostatin at baseline correlated negatively with insulin sensitivity. Myostatin expression in the adipose tissue increased after 12 weeks of training and correlated positively with insulin sensitivity markers. In cultured muscle cells but not in SGBS cells, myostatin promoted an insulin-independent increase in glucose uptake. Furthermore, muscle cells incubated with myostatin had an enhanced rate of glucose oxidation and lactate production. CONCLUSION: Myostatin was differentially expressed in the muscle and adipose tissue in relation to physical activity and dysglycaemia. Recombinant myostatin increased the consumption of glucose in human skeletal muscle cells, suggesting a complex regulatory role of myostatin in skeletal muscle homeostasis.

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Crossref · 10.1111/apha.12631

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Authors
Hjorth, M., Pourteymour, S., Görgens, S. W., Langleite, T. M., Lee, S., Holen, T., Gulseth, H. L., Birkeland, K. I., Jensen, J., Drevon, C. A., & Norheim, F.
Affiliations
  • University of Oslo (NO)
  • Deutsches Diabetes-Zentrum e.V. (DE)
  • Oslo University Hospital (NO)
  • Department of Nutrition Institute of Basic Medical Sciences Faculty of Medicine University of Oslo Oslo Norway
  • Norwegian School of Sport Sciences (NO)
Corresponding author
Hjorth, M.
Venue
Acta Physiologica
Publisher
Wiley
Published
2015-12-09
Volume
217
Issue
1
Pages
45-60
ISSN
  • 1748-1708 (ISSN-L)
  • 1748-1716
OpenAlex
W2180688893
Cited by
83
References
85
Citations by year
2026: 7 · 2025: 5 · 2024: 15 · 2023: 8 · 2022: 10 · 2021: 7 · 2020: 4 · 2019: 6 · 2018: 10 · 2017: 7 · 2016: 4
FWCI
3.04
Type
journal article
Language
en
Open access
closed
Locations
  • Acta Physiologica (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Universitetet i Oslo
  • Helse Sør-Øst RHF
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
  • Health Sciences › Medicine › Rehabilitation › Exercise and Physiological Responses
  • Health Sciences › Medicine › Epidemiology › Adipokines, Inflammation, and Metabolic Diseases
Keywords
myostatin, myokines, physical activity, dysglycemia, insulin sensitivity, skeletal muscle, adipose tissue, energy metabolism, glucose uptake, glucose oxidation, lactic acid production, vastus lateralis
MeSH terms
Adipose Tissue, Adult, Aged, Arrhythmias, Cardiac, Blood Glucose, Energy Metabolism, Gigantism, Glucose, Heart Defects, Congenital, Humans, Insulin Resistance, Male, Intellectual Disability, Middle Aged, Blotting, Western, Glucose Clamp Technique, Exercise, Down-Regulation, Muscle, Skeletal, Reverse Transcriptase Polymerase Chain Reaction, Muscle Cells, Genetic Diseases, X-Linked, Myostatin, High-Throughput Nucleotide Sequencing

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