Degradation of Chondroitin Sulfate Proteoglycans Induces Sprouting of Intact Purkinje Axons in the Cerebellum of the Adult Rat
Open accessAbstract
Chondroitin sulfate proteoglycans are major constituents of the extracellular matrix and form perineuronal nets. Information regarding the growth-inhibitory activity of these molecules after injury is rapidly expanding. However, less is known about their physiological role in the adult undamaged CNS. Here, we investigated the function of chondroitin sulfate proteoglycans in maintaining the proper structure of Purkinje axons in the cerebellum of adult rats. To this end, we examined the morphology and distribution of intracortical Purkinje neurites after intraparenchymal injection of chondroitinase ABC. Staining with the lectin Wisteria floribunda agglutinin or 2B6 antibodies showed that this treatment efficiently removed chondroitin sulfate proteoglycans from wide areas of the cerebellar cortex. In the same sites, there was a profuse outgrowth of terminal branches from the Purkinje infraganglionic plexus, which invaded the deeper regions of the granular layer. In contrast, myelinated axon segments were not affected and maintained their normal relationship with oligodendroglial sheaths. Purkinje axon sprouting was first evident at 4 d and increased further at 7 d after enzyme application. Within 42 d, the expression pattern of chondroitin sulfate proteoglycans gradually recovered, whereas axonal modifications progressively regressed. Our results show that, in the absence of injury or novel external stimuli, degradation of chondroitin sulfate proteoglycans is sufficient to induce Purkinje axon sprouting but not the formation of long-lasting synaptic contacts. Together with other growth-inhibitory molecules, such as myelin-associated proteins, chondroitin sulfate proteoglycans restrict structural plasticity of intact Purkinje axons to maintain normal wiring patterns in the adult cerebellar cortex.
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Purkinje cell terminals sprout after ChABC digestion in the deep cerebellar nucleus.
“Purkinje cell terminals sprout after ChABC digestion [48]”
From The extracellular matrix and perineuronal nets in memory (2022) · cites this paper as [48]
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- Authors
- Corvetti, L., & Rossi, F.
- Affiliations
- University of Turin (IT)
- Venue
- The Journal of Neuroscience
- Publisher
- Society for Neuroscience
- Published
- 2005-08-03
- Volume
- 25
- Issue
- 31
- Pages
- 7150-7158
- ISSN
- 0270-6474 (ISSN-L)
- 1529-2401
- PMC
- PMC6725229
- OpenAlex
- W2089089729
- Cited by
- 138
- References
- 2
- Citations by year
- 2026: 1 · 2025: 4 · 2024: 5 · 2023: 6 · 2022: 6 · 2021: 6 · 2020: 10 · 2019: 3 · 2018: 5 · 2017: 8 · 2016: 6 · 2015: 5 · 2014: 7 · 2013: 7 · 2012: 7
- FWCI
- 4.34
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Neuroscience (publishedVersion, cc-by-nc-sa)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Institutional Research Information System University of Turin (University of Turin) (submittedVersion)
- Funders
- Università degli Studi di Torino
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Proteoglycans and glycosaminoglycans research
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Nerve injury and regeneration
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Keywords
- chondroitin sulfate proteoglycans, axonal sprouting, chondroitinase ABC, cerebellum, perineuronal nets, extracellular matrix, growth inhibition, structural plasticity, granular layer, myelinated axons, oligodendrocytes
- MeSH terms
- Animals, Axons, Cerebellum, Myelin Sheath, Nerve Regeneration, Chondroitin Sulfate Proteoglycans, Purkinje Cells, Tissue Distribution, Rats, Wistar, Chondroitin ABC Lyase, Rats
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