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Cited by 34Proteoglycans and glycosaminoglycans researchNeuroscience and Neuropharmacology ResearchNeurogenesis and neuroplasticity mechanisms

Total number, distribution, and phenotype of cells expressing chondroitin sulfate proteoglycans in the normal human amygdala

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Pantazopoulos, Harry, Murray, Elisabeth A., Berretta, Sabina·2008·Brain Research
DOI 10.1016/j.brainres.2008.02.036PMC PMC2696935
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Abstract

Chondroitin sulfate proteoglycans (CSPGs) are a key structural component of the brain extracellular matrix. They are involved in critical neurodevelopmental functions and are one of the main components of pericellular aggregates known as perineuronal nets. As a step toward investigating their functional and pathophysiological roles in the human amygdala, we assessed the pattern of CSPG expression in the normal human amygdala using wisteria floribunda agglutinin (WFA) lectin histochemistry. Total numbers of WFA-labeled elements were measured in the lateral (LN), basal (BN), accessory basal (ABN) and cortical (CO) nuclei of the amygdala from 15 normal adult human subjects. For interspecies qualitative comparison, we also investigated the pattern of WFA labeling in the amygdala of naïve rats (n=32) and rhesus monkeys (Macaca mulatta; n=6). In human amygdala, WFA lectin histochemistry resulted in labeling of perineuronal nets and cells with clear glial morphology, while neurons did not show WFA labeling. Total numbers of WFA-labeled glial cells showed high interindividual variability. These cells aggregated in clusters with a consistent between-subjects spatial distribution. In a subset of human subjects (n=5), dual color fluorescence using an antibody raised against glial fibrillary acidic protein (GFAP) and WFA showed that the majority (93.7%) of WFA-labeled glial cells correspond to astrocytes. In rat and monkey amygdala, WFA histochemistry labeled perineuronal nets, but not glial cells. These results suggest that astrocytes are the main cell type expressing CSPGs in the adult human amygdala. Their highly segregated distribution pattern suggests that these cells serve specialized functions within human amygdalar nuclei.

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Crossref · 10.1016/j.brainres.2008.02.036

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  • A large number of 3B3-IR PNNs were detected throughout the amygdala, outnumbering aggrecan-IR PNNs and WFA-positive PNNs, particularly within the basal nucleus, accessory basal nucleus, cortical nucleus and central/medial nuclei.

    “A large number of 3B3-IR PNNs were detected throughout the amygdala, outnumbering by far aggrecan-IR PNNs (Supplementary Tables 4 and 5) as well as WFA-positive PNNs,3, 67 particularly within the BN, ABN, CO and CE/ME”

    From Aggrecan and chondroitin-6-sulfate abnormalities in schizophrenia and bipolar disorder: a postmortem study on the amygdala (2015) · cites this paper as [67]

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Authors
Pantazopoulos, H., Murray, E. A., & Berretta, S.
Affiliations
  • McLean Hospital (US)
  • National Institutes of Health (US)
  • Harvard University (US)
Corresponding author
Berretta, Sabina
ORCID
Venue
Brain Research
Publisher
Elsevier BV
Published
2008-05
Volume
1207
Pages
84-95
ISSN
0006-8993 (ISSN-L)
DOI
10.1016/j.brainres.2008.02.036
PMC
PMC2696935
OpenAlex
W2004034825
Cited by
34
References
92
Citations by year
2026: 1 · 2024: 3 · 2021: 2 · 2019: 2 · 2018: 3 · 2017: 3 · 2016: 5 · 2015: 2 · 2014: 3 · 2012: 2
FWCI
1.17
Type
journal article
Language
en
Open access
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Brain Research (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • National Institutes of Health (5r03mh063215-02)
  • National Institute of Mental Health (MH063215, R24MH068855, R01MH066280, MH 066280, R01MH066955)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Proteoglycans and glycosaminoglycans research
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
Keywords
chondroitin sulfate proteoglycans, amygdala, perineuronal nets, astrocytes, glial fibrillary acidic protein, Wisteria floribunda agglutinin, brain extracellular matrix, amygdala nuclei, interindividual variability, human amygdala
MeSH terms
Adult, Aged, Aged, 80 and over, Amygdala, Animals, Haplorhini, Astrocytes, Cell Count, Female, Glial Fibrillary Acidic Protein, Humans, Male, Middle Aged, Phenotype, Postmortem Changes, Chondroitin Sulfate Proteoglycans, Rats, Sprague-Dawley, Receptors, N-Acetylglucosamine, Plant Lectins, Rats

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