Redundant Localization Mechanisms of RIM and ELKS in Caenorhabditis elegans
Open accessAbstract
Active zone proteins play a fundamental role in regulating neurotransmitter release and defining release sites. The functional roles of active zone components are beginning to be elucidated; however, the mechanisms of active zone protein localization are unknown. Studies have shown that glutamine, leucine, lysine, and serine-rich protein (ELKS), a recently defined member of the active zone complex, acts to localize the active zone protein Rab3a-interacting molecule (RIM) and regulates synaptic transmission in cultured neurons. Here, we test the function of ELKS in vivo. Like mammalian ELKS, Caenorhabditis elegans ELKS is an active zone protein that directly interacts with the postsynaptic density-25/Discs large/zona occludens (PDZ) domain of RIM. However, RIM protein localizes in the absence of ELKS and vice versa. In addition, elks mutants exhibit neither the behavioral nor the physiological defects associated with unc-10 RIM mutants, indicating that ELKS is not a critical component of the C. elegans release machinery. Interestingly, expression of the soluble PDZ domain of RIM disrupts ELKS active zone targeting, suggesting a tight association between the two proteins in vivo. RIM truncations containing only the PDZ and C2A domains target to release sites in an ELKS-dependent manner. Together, these data identify ELKS as a new member of the C. elegans active zone complex, define the role of ELKS in synaptic transmission, and characterize the relationship between ELKS and RIM in vivo. Furthermore, they demonstrate that multiple different protein-protein interactions redundantly anchor both ELKS and RIM to active zones and implicate novel proteins in the formation of the active zone.
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Deletion of ELKS in C. elegans and of ELKS2 in mice did not impair neurotransmitter release.
“deletion of ELKS in C. elegans and of ELKS2 in mice did not impair neurotransmitter release (Deken et al., 2005; Kaeser et al., 2009).”
From The Presynaptic Active Zone (2012) · cites this paper as Deken et al., 2005
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Macromolecular complexes at active zones: integrated nano-machineries for neurotransmitter release
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The Yin and Yang of Synaptic Active Zone Assembly
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Voltage-Gated Calcium Channels (VGCCs) and Synaptic Transmission
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Presynaptic Cytomatrix Proteins
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Ndm, a coiled-coil domain protein that suppresses macropinocytosis and has effects on cell migration
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Characterization of synaptic protein complexes in Drosophila melanogaster
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Blobby is a synaptic active zone assembly protein required for memory in Drosophila
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Network of Protein-Protein Interactions at the Presynaptic Active Zone
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Structure and Function of Vertebrate and Invertebrate Active Zones
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Liprins, ELKS, and RIM-BP Proteins
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Early Active Zone Assembly in Drosophila
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Characterization of synaptic protein complexes in Drosophila
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A transcriptional program remodels GABAergic synapses in C. elegans
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Presynaptic Mechanisms for Cargo Recruitment
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Roles of the ELKS/CAST Family and SAD Kinase in Neurotransmitter Release
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Liprins, ELKS, and RIM-BP Proteins
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Identification and characterization of a synaptic active zone assembly proteinPreprint
Lützkendorf, J., Matkovic, T., Götz, T., Liu, S., Ghelani, T., Maglione, M., Grieger, M., Putignano, S., Gao, L., Gimber, N., et al. (2024) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2024.04.08.588536
A modular system to label endogenous presynaptic proteins using split fluorophores in C. elegansPreprint
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CAST/ELKS–endophilin-A interaction ensures synaptic vesicle pool size
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- Authors
- Deken, S. L., Vincent, R., Hadwiger, G., Liu, Q., Wang, Z.-W., & Nonet, M. L.
- Affiliations
- Washington University in St. Louis (US)
- UConn Health (US)
- Corresponding author
- Nonet, Michael L.
- Venue
- The Journal of Neuroscience
- Publisher
- Society for Neuroscience
- Published
- 2005-06-22
- Volume
- 25
- Issue
- 25
- Pages
- 5975-5983
- ISSN
- 0270-6474 (ISSN-L)
- 1529-2401
- PMC
- PMC6724794
- OpenAlex
- W2029712139
- Cited by
- 72
- References
- 48
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 3 · 2023: 4 · 2022: 1 · 2021: 3 · 2020: 2 · 2019: 3 · 2018: 6 · 2017: 4 · 2016: 1 · 2015: 3 · 2014: 4 · 2013: 4 · 2012: 6
- FWCI
- 1.45
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Neuroscience (publishedVersion, cc-by-nc-sa)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (1f32ns047000-01)
- National Institute of Neurological Disorders and Stroke (5 F32 NS047000, R01NS033535)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurobiology and Insect Physiology Research
- Keywords
- RIM, elk, active zone proteins, protein localization, PDZ domain, synaptic transmission, neurotransmitter release, Caenorhabditis elegans, unc-10, protein-protein interaction, release sites
- MeSH terms
- Animals, Carrier Proteins, Cloning, Molecular, Electrophysiology, Synaptic Transmission, Synapses, Caenorhabditis elegans, Caenorhabditis elegans Proteins, Intracellular Signaling Peptides and Proteins
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