Integration of Asynchronously Released Quanta Prolongs the Postsynaptic Spike Window
Open accessAbstract
Classically, the release of glutamate in response to a presynaptic action potential causes a brief increase in postsynaptic excitability. Previous reports indicate that at some central synapses, a single action potential can elicit multiple, asynchronous release events. This raises the possibility that the temporal dynamics of neurotransmitter release may determine the duration of altered postsynaptic excitability. In response to physiological challenges, the magnocellular neurosecretory cells (MNCs) in the paraventricular nucleus of the hypothalamus (PVN) exhibit robust and prolonged increases in neuronal activity. Although the postsynaptic conductances that may facilitate this form of activity have been investigated thoroughly, the role of presynaptic release has been largely overlooked. Because the specific patterns of activity generated by MNCs require the activation of excitatory synaptic inputs, we sought to characterize the release dynamics at these synapses and determine whether they contribute to prolonged excitability in these cells. We obtained whole-cell recordings from MNCs in brain slices of postnatal day 21-44 rats. Stimulation of glutamatergic inputs elicited large and prolonged postsynaptic events that resulted from the summation of multiple, asynchronously released quanta. Asynchronous release was selectively inhibited by the slow calcium buffer EGTA-AM and potentiated by brief high-frequency stimulus trains. These trains caused a prolonged increase in postsynaptic spike activity that could also be eliminated by EGTA-AM. Our results demonstrate that glutamatergic terminals in PVN exhibit asynchronous release, which is important in generating large postsynaptic depolarizations and prolonged spiking in response to brief, high-frequency bursts of presynaptic activity.
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Persistent activity seems to be a hallmark of hypothalamic neurons, which can be highly interconnected and have long integration periods.
“Persistent activity seems to be a hallmark of hypothalamic neurons, which can be highly interconnected and have long integration periods42–45.”
From Neural circuitry for maternal oxytocin release induced by infant cries (2023) · cites this paper as [42]
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- Authors
- Iremonger, K. J., & Bains, J. S.
- Affiliations
- University of Calgary (CA)
- Corresponding author
- Bains, J. S.
- Venue
- Journal of Neuroscience
- Publisher
- Society for Neuroscience
- Published
- 2007-06-20
- Volume
- 27
- Issue
- 25
- Pages
- 6684-6691
- ISSN
- 0270-6474 (ISSN-L)
- 1529-2401
- PMC
- PMC6672686
- OpenAlex
- W2108193936
- Cited by
- 89
- References
- 50
- Citations by year
- 2026: 1 · 2025: 4 · 2024: 3 · 2023: 7 · 2022: 3 · 2021: 4 · 2020: 4 · 2019: 5 · 2018: 3 · 2017: 3 · 2016: 2 · 2015: 4 · 2014: 9 · 2013: 3 · 2012: 6
- FWCI
- 2.51
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of Neuroscience (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Fondation pour la Recherche Médicale
- Alberta Heritage Foundation for Medical Research
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Life Sciences › Neuroscience › Cognitive Neuroscience › Neural dynamics and brain function
- Keywords
- asynchronous neurotransmitter release, glutamate release, postsynaptic excitability, magnocellular neurosecretory cells, paraventricular nucleus, glutamatergic synapses, synaptic summation
- MeSH terms
- Action Potentials, Animals, Male, Synapses, Synaptic Vesicles, Time Factors, Rats, Sprague-Dawley, Rats, Wistar, Excitatory Postsynaptic Potentials, Animals, Genetically Modified, Rats
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