The DOI matches its Crossref record; 98 of the 99 references it cites were found in a registry or an index; 28 of the 70 sources its claims cite were checked for support.
Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease
Open accessAbstract
Alzheimer's disease is associated with a disruption of amyloid β (Aβ) homeostasis, resulting in the accumulation and subsequent deposition of Aβ peptides within the brain. The peroxisome proliferator-activated receptor-γ (PPARγ) is a ligand-activated nuclear receptor that acts in a coupled metabolic cycle with Liver X Receptors (LXRs) to increase brain apolipoprotein E (apoE) levels. apoE functions to promote the proteolytic clearance of soluble forms of Aβ, and we found that the synthetic PPARγ agonist, pioglitazone, stimulated Aβ degradation by both microglia and astrocytes in an LXR and apoE-dependent manner. Remarkably, a brief 9 d oral treatment of APPswe/PS1Δe9 mice with pioglitazone resulted in dramatic reductions in brain levels of soluble and insoluble Aβ levels which correlated with the loss of both diffuse and dense-core plaques within the cortex. The removal of preexisting amyloid deposits was associated with the appearance of abundant Aβ-laden microglia and astrocytes. Pioglitazone treatment resulted in the phenotypic polarization of microglial cells from a proinflammatory M1 state, into an anti-inflammatory M2 state that was associated with enhanced phagocytosis of deposited forms of amyloid. The reduction in amyloid levels was associated with a reversal of contextual memory deficits in the drug-treated mice. These data provide a mechanistic explanation for how PPARγ activation facilitates amyloid clearance and supports the therapeutic utility of PPARγ agonists for the treatment of Alzheimer's disease.
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TL;DR
Pioglitazone, a PPARγ agonist, rapidly cleared soluble and deposited amyloid-β in APP/PS1 mice through PPARγ–LXRα–apoE-dependent degradation and M2 microglial polarization. A 9 d treatment reversed contextual memory deficits, supporting PPARγ agonists as a therapeutic approach for Alzheimer’s disease.
Key findings
- In primary microglia and astrocytes, pioglitazone stimulated dose-dependent intracellular degradation of soluble Aβ42; this was blocked by PPARγ antagonist T0070907 and LXR antagonist 22HC, impaired in apoe−/− cells, and restored by exogenous ApoE or ApoA1.
- PPARγ conditional knockout mice showed >70% lower cortical pparγ mRNA and >50% reductions in cortical ABCA1 and ApoE protein, demonstrating a PPARγ–LXR linkage in brain.
- In 6- and 12-month-old APP/PS1 mice, 9 d oral pioglitazone (80 mg·kg−1·d−1) reduced 6E10 plaque area by ~40% and ~30%, and thioS+ dense-core plaques by ~40% and ~30%, respectively; at 6 months soluble and insoluble Aβ fell by ~50%, while at 12 months soluble Aβ40 fell 40% and insoluble Aβ40/Aβ42 fell 50%/40%, with no significant change in soluble Aβ42.
- Treatment increased M2 markers (Ym1 at 6 months; Fizz1, Ym1, Arg1 at 12 months), reduced Il-1β and tnfα at 12 months, and reversed contextual memory deficits to wild-type levels; >90% of cortical microglia in drug-treated animals internalized amyloid by qualitative assessment.
Methods
- Primary microglia and astrocytes from postnatal C57BL/6J or apoe−/− pups were treated with pioglitazone ± T0070907, 22HC, ApoE, or ApoA1; Aβ levels were measured by ELISA, with Western blot and qPCR for related genes/proteins.
- APP/PS1 and wild-type littermates at 6 and 12 months were gavaged with pioglitazone (80 mg·kg−1·d−1) or vehicle for 9 d; PPARγ fl/fl × GFAP-cre mice were used for conditional knockout. Brains were analyzed by immunohistochemistry (6E10, thioflavin S, Iba1, CD45, GFAP), Aβ ELISA, qPCR, and Western blot.
- Contextual fear conditioning was performed in 12-month-old mice after 9 d treatment, with open-field testing for noncognitive behavior; plaque counts were done by a blinded observer and qPCR used the ΔΔCT method normalized to GAPDH.
Limitations
- The paper states pioglitazone is poorly blood-brain barrier permeable, necessitating 80 mg·kg−1·d−1, a dose higher than previously administered and ~8 times the clinically approved dose, with a very short 9 d treatment interval.
- The paper notes astrocyte Aβ quantification was limited by the GFAP marker, astrocyte cell numbers surrounding plaques could not be determined, and Aβ-positive astrocytes represented only a minor subset that was not quantitatively evaluated; microglial Aβ uptake at 6 months was difficult to assess due to few Iba1+ cells.
- Evident from the text: the mechanistic and behavioral conclusions derive from an APP/PS1 mouse model and primary rodent glia, with several microglial/astrocytic findings based on qualitative imaging rather than fully quantitative causal assays.
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Claims the paper makes
62 Own result · 31 Own interpretation · 15 Partly supported · 10 No full text of the sources · 4 Supported · 4 Sources not found · 3 Own method · 2 Not checked yet · 1 Supported so far · accuracy not yet measured
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In the paper
“Alzheimer's disease (AD) is characterized by the accumulation and deposition of amyloid β (Aβ) within the brain because of inefficient clearance of these peptides (Mawuenyega et al., 2010; Querfurth and LaFerla, 2010).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Introduction
What the sources say
- Partly supportedcites Mawuenyega et al., 2010 sourceExperimental — accuracy not yet measured
The paper reports increased amyloid-β in Alzheimer’s disease and slower clearance of Aβ42 and Aβ40 in patients than in controls (Abstract and study results; page not supplied). But it explicitly says the association of impaired clearance with Alzheimer’s disease does not prove causality, so the claim’s unqualified “because of” goes beyond its findings. (LLM, conf=0.88)
- Not checked — no full text of the sourcecites Querfurth and LaFerla, 2010 source
plaindirect- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: passes — The paper’s conclusions depend on it.
- Based: passes — The paper gives a reason why it holds.
- Partly supported
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In the paper
“Aβ accumulation is associated with perturbations in synaptic function, neuronal loss, and memory and cognitive impairments (Lue et al., 1999; Akiyama et al., 2000b; Palop and Mucke, 2010).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Introduction
What the sources say
- Partly supportedcites Lue et al., 1999 sourceExperimental — accuracy not yet measured
The Abstract reports that soluble Aβ concentrations were strongly associated with synapse loss, supporting the synaptic part of the claim. But it says insoluble Aβ deposits were poor correlates of synaptic change, so the finding does not apply to Aβ accumulation indiscriminately. The Patient Samples section says the study focused on synapse loss rather than dementia itself; it does not establish an association with neuronal loss or measured memory and cognitive impairment. (LLM, conf=0.88)
- Not matchedcites Akiyama et al., 2000b
Couldn’t be matched to a paper in Crossref, so it can’t be checked.
- Partly supportedcites Palop and Mucke, 2010 sourceExperimental — accuracy not yet measured
The Introduction and “Modulation of synaptic transmission by Aβ” sections link elevated Aβ to impaired synaptic function and synaptic loss. “Elevated Aβ destabilizes neural network activity” links these changes to learning, memory, and cognitive deficits. The passages describe loss of synapses, not loss of neurons, so they do not support that part of the claim; page numbers were not provided in our copy. (LLM, conf=0.86)
plaincorrelational- Asserted: passes — The authors put it forward as true.
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- Partly supported
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In the paper
“Aβ deposition elicits a robust “M1” microglia-mediated inflammatory response contributing to disease pathogenesis (Gordon, 2003; Mosser and Edwards, 2008; Mandrekar-Colucci and Landreth, 2010).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Introduction
What the sources say
- Not checked — no full text of the sourcecites Gordon, 2003 source
- Partly supportedcites Mosser and Edwards, 2008 sourceExperimental — accuracy not yet measured
The ‘Classically activated macrophages’ section says their pro-inflammatory cytokines can damage the host and contribute to inflammatory pathology. The paper also mentions CNS microglia in ‘The origin and maintenance of macrophages,’ but does not say that Aβ deposition triggers an M1 microglial response or that such a response contributes to disease pathogenesis. No page numbers are available in our copy. (LLM, conf=0.85)
- Supportedcites Mandrekar-Colucci and Landreth, 2010 source
The Introduction describes amyloid deposition as associated with a robust inflammatory response, and “Microglia and Inflammation in AD” describes pro-inflammatory molecules released by microglia in response to Aβ. “Alternative Activation of Microglial Cells” identifies M1 as the pro-inflammatory phenotype and links Aβ recognition to M1 activation. The paper also says inflammation contributes to AD progression, while noting that microglia can have protective roles. (LLM, conf=0.87)
plaindirect- Asserted: passes — The authors put it forward as true.
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The cited sources support only part of this claim — read each source’s verdict below.
In the paper
“Sustained PPARγ or LXR activation results in amelioration of AD-related pathophysiology in AD mouse models (Yan et al., 2003; Pedersen and Flynn, 2004; Heneka et al., 2005; Koldamova et al., 2005a; Riddell et al., 2007; Zelcer et al., 2007; Jiang et al., 2008; Donkin et al., 2010; Fitz et al., 2010; Toledo and Inestrosa, 2010).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Introduction
What the sources say
- Partly supportedcites Yan et al., 2003 sourceExperimental — accuracy not yet measured
The Results report that four months of treatment with the PPARγ agonist pioglitazone significantly reduced SDS-soluble Aβ40 in Tg2576 mice. The Introduction and Discussion say it did not significantly reduce plaque burden or microglial activation, so the broader claim of ameliorated AD-related pathology overstates this study. The paper does not assess LXR activation; page numbers were not provided for these passages. (LLM, conf=0.86)
- Not checked — no full text of the sourcecites Pedersen and Flynn, 2004 source
- Partly supportedcites Heneka et al., 2005 sourceExperimental — accuracy not yet measured
The Introduction (page unavailable) reports that pioglitazone, a PPARγ agonist, reduced inflammatory measures and Aβ deposition in APP-overexpressing mice. But the Animals section (page unavailable) specifies only seven days of treatment, so this paper supports an acute effect, not the claimed sustained activation. It does not report LXR activation. (LLM, conf=0.88)
plaindirect- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
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- Partly supported
The cited sources support only part of this claim — read each source’s verdict below.
In the paper
“Peripherally, LXRs and PPARγ participate in a coupled metabolic pathway, whereby activation of PPARγ induces expression of LXRα and its target genes abca1 and apoE (Chawla et al., 2001; Seo et al., 2004; Yue and Mazzone, 2009).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Introduction
What the sources say
- Partly supportedcites Chawla et al., 2001 sourceExperimental — accuracy not yet measured
The Introduction and Figures 1–3 (page numbers unavailable) support a PPARγ–LXRα pathway in macrophages: PPARγ activation induces LXRα, and PPARγ and LXR ligands cooperate to induce ABCA1. The passages identify ABCG1, not apoE, as the other induced gene; our copy does not support the attributed apoE induction. (LLM, conf=0.88)
- Partly supportedcites Seo et al., 2004 sourceExperimental — accuracy not yet measured
The Discussion (page unavailable) says PPARγ regulates LXRα expression and describes a positive-feedback pathway between the two receptors. An introductory passage indexed as Abstract (page unavailable) says LXR activation promotes cholesterol efflux through ABCA1 and apoE in macrophages. The paper does not establish the full claimed sequence—that activating PPARγ induces expression of LXRα, ABCA1, and apoE. (LLM, conf=0.82)
- Not checked — no full text of the sourcecites Yue and Mazzone, 2009 source
plaindirect- Asserted: passes — The authors put it forward as true.
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In the paper
“It has recently been appreciated that PPARs act as master regulators governing the polarization of macrophages and microglia into “M2” or “alternative” activation states associated with the suppression of inflammation and promotion of phagocytosis and tissue repair (Chawla, 2010; Chinetti-Gbaguidi et al., 2011).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Introduction
What the sources say
- Partly supportedcites Chawla, 2010 sourceExperimental — accuracy not yet measured
The ‘PPARs and alternative macrophage activation’ section (page not supplied) says PPARγ and PPARδ are required to acquire and maintain the alternative macrophage phenotype and links PPARδ to anti-inflammatory effects. ‘Macrophage activation and functional heterogeneity’ (page not supplied) associates that phenotype with phagocytic receptors and reparative functions. But the paper identifies IL-4 and IL-13 as the instructive cues, rather than PPARs as master regulators, and the passages found do not extend the claim to microglia. (LLM, conf=0.87)
- Not checked — no full text of the sourcecites Chinetti-Gbaguidi et al., 2011 source
plaindirect- Asserted: passes — The authors put it forward as true.
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- Partly supported
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In the paper
“The actions of PPARγ in the brain of murine models of AD have been controversial, largely due to the poor penetrance of its agonists into the brain and active P-glycoprotein-mediated efflux mechanisms (Maeshiba et al., 1997; Hemauer et al., 2010).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Discussion
What the sources say
- Not matchedcites Maeshiba et al., 1997
Couldn’t be matched to a paper in Crossref, so it can’t be checked.
- Partly supportedcites Hemauer et al., 2010 sourceExperimental — accuracy not yet measured
The COMMENT section (page not available) says P-glycoprotein transports rosiglitazone from brain to blood, supporting an efflux mechanism for that agonist. The paper does not establish poor brain penetration of PPARγ agonists generally or link that mechanism to controversy about PPARγ actions in murine Alzheimer’s disease models. (LLM, conf=0.86)
plaindirect- Asserted: passes — The authors put it forward as true.
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- Not matched
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In the paper
“Thus, modulating microglial activation status by targeting nuclear receptors appears to restore the competence of these cells to phagocytose and clear amyloid in the AD brain (Mukundan et al., 2009; Chinetti-Gbaguidi et al., 2011).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Discussion
What the sources say
- Partly supportedcites Mukundan et al., 2009 sourceExperimental — accuracy not yet measured
The Results section ('PPAR-δ regulates opsonin gene expression in macrophages'; page unavailable) reports that activating the nuclear receptor PPAR-δ increased macrophage uptake of apoptotic cells. The paper does not establish that targeting nuclear receptors restores microglial clearance of amyloid in an Alzheimer’s disease brain; its experiments concern macrophages and apoptotic cells. (LLM, conf=0.88)
- Not checked — no full text of the sourcecites Chinetti-Gbaguidi et al., 2011 source
hedgedconditional- Asserted: passes — The authors put it forward as true.
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In the paper
“Nuclear receptors have only recently been appreciated to play an crucial role in promoting the phenotypic change of macrophages from a “classically” M1 activated to alternatively activated M2 states (Odegaard et al., 2007, 2008; Zelcer et al., 2007; Bouhlel et al., 2008; Kalinin et al., 2009; Chawla, 2010).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Discussion
What the sources say
- Not matchedcites Odegaard et al., 2007, 2008
Couldn’t be matched to a paper in Crossref, so it can’t be checked.
- Partly supportedcites Zelcer et al., 2007 sourceExperimental — accuracy not yet measured
The Abstract says LXRs are nuclear receptors that inhibit inflammatory responses in macrophages, and the Results show that LXR activation suppresses inflammatory responses in microglia. Neither passage establishes the claimed phenotypic change of macrophages from M1 to M2 states. (LLM, conf=0.82)
- Not checked — no full text of the sourcecites Bouhlel et al., 2008 source
plaindirect- Asserted: passes — The authors put it forward as true.
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- Not matched
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In the paper
“However, phase III clinical trials, using rosiglitazone, failed to show efficacy for the treatment of AD (Gold et al., 2010).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Discussion
What the sources say
- Partly supportedcites Gold et al., 2010 sourceExperimental — accuracy not yet measured
The Introduction describes one phase III rosiglitazone trial in people with mild-to-moderate AD, and the Conclusion reports no evidence of efficacy. The cited paper supports that result for its trial, but not the claim that multiple phase III trials failed to show efficacy. (LLM, conf=0.86)
plain- Asserted: passes — The authors put it forward as true.
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- Partly supported
A result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“Remarkably, a brief 9 d oral treatment of APPswe/PS1Δe9 mice with pioglitazone resulted in dramatic reductions in brain levels of soluble and insoluble Aβ levels which correlated with the loss of both diffuse and dense-core plaques within the cortex.”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Abstract
boosteddirect- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: passes — The paper’s conclusions depend on it.
- Based: passes — The paper gives a reason why it holds.
A result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“we found that the synthetic PPARγ agonist, pioglitazone, stimulated Aβ degradation by both microglia and astrocytes in an LXR and apoE-dependent manner.”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Abstract
plaindirect- Asserted: passes — The authors put it forward as true.
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A result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“At 6 months of age, Fizz1 levels were significantly reduced in vehicle- and pioglitazone-treated APP/PS1 animals.”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Results > Pioglitazone suppresses glial activation and enhances amyloid clearance
plaindirect- Asserted: passes — The authors put it forward as true.
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A result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“soluble levels of Aβ40 were reduced by 40%”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Results > PPARγ activation rapidly stimulates the clearance of Aβ in a mouse model of AD
plaindirect- Asserted: passes — The authors put it forward as true.
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In the paper
“At 12 months of age, no significant change was seen in levels of soluble Aβ42; however, soluble levels of Aβ40 were reduced by 40% and insoluble levels of both Aβ40 and Aβ42 were reduced by 50% and 40%, respectively (Fig. 4K,L).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Results > PPARγ activation rapidly stimulates the clearance of Aβ in a mouse model of AD
plain- Asserted: passes — The authors put it forward as true.
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A result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“The reduction in amyloid levels was associated with a reversal of contextual memory deficits in the drug-treated mice.”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Abstract
plaincorrelational- Asserted: passes — The authors put it forward as true.
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A result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“Loss of apoe resulted in a significant impairment of intracellular sAβ degradation by both cell types in response to pioglitazone treatment (Fig. 2C,D).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Results > PPARγ facilitates the degradation of soluble Aβ through an LXR/ApoE-dependent pathway
plaindirect- Asserted: passes — The authors put it forward as true.
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In the paper
“Remarkably, at 12 months of age, we found that most microglia in pioglitazone-treated APP/PS1 animals exhibited robust internalization of Aβ (Fig. 7C,D).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Results > Pioglitazone suppresses glial activation and enhances amyloid clearance
plaincorrelational- Asserted: passes — The authors put it forward as true.
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In the paper
“Pioglitazone, however, dramatically increased levels of the alternative activation marker, Ym1 mRNA in 6-month-old APP/PS1 animals (Fig. 7E).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Results > Pioglitazone suppresses glial activation and enhances amyloid clearance
boosteddirect- Asserted: passes — The authors put it forward as true.
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- Load-bearing: passes — The paper’s conclusions depend on it.
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A result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“The removal of preexisting amyloid deposits was associated with the appearance of abundant Aβ-laden microglia and astrocytes.”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Abstract
plaincorrelational- Asserted: passes — The authors put it forward as true.
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A result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“Exogenous ApoE or ApoA1 stimulated the clearance of Aβ peptides from the media (Fig. 2E,F).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Results > PPARγ facilitates the degradation of soluble Aβ through an LXR/ApoE-dependent pathway
plaindirect- Asserted: passes — The authors put it forward as true.
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- Load-bearing: passes — The paper’s conclusions depend on it.
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A result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“Based on qualitative immunohistological evaluation, we found abundant microglia closely associated with plaque cores in vehicle-treated animals (Fig. 6A–C,G–I).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Results > Pioglitazone suppresses glial activation and enhances amyloid clearance
plaincorrelational- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: unclear — It is unclear whether the paper’s conclusions depend on it.
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A result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“into an anti-inflammatory M2 state that was associated with enhanced phagocytosis of deposited forms of amyloid.”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Abstract
plaincorrelational- Asserted: passes — The authors put it forward as true.
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- Load-bearing: passes — The paper’s conclusions depend on it.
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A result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“insoluble levels of both Aβ40 and Aβ42 were reduced by 50% and 40%, respectively (Fig. 4K,L).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Results > PPARγ activation rapidly stimulates the clearance of Aβ in a mouse model of AD
plaindirect- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: passes — The paper’s conclusions depend on it.
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A result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“Surprisingly, internalized Aβ peptides were found only in astrocytes of pioglitazone-treated animals, as seen in z-stack images obtained by confocal microscopy (Fig. 5M).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Results > Pioglitazone suppresses glial activation and enhances amyloid clearance
plain- Asserted: passes — The authors put it forward as true.
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A result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“Remarkably, at 6 months of age, only 9 d of pioglitazone treatment reduced soluble and insoluble levels of Aβ by ~50% (Fig. 4G,H).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Results > PPARγ activation rapidly stimulates the clearance of Aβ in a mouse model of AD
plaindirect- Asserted: passes — The authors put it forward as true.
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A result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“It is our qualitative assessment that >90% of the microglia in the cortex of drug-treated animals internalized vast quantities of amyloid.”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Results > Pioglitazone suppresses glial activation and enhances amyloid clearance
hedged- Asserted: passes — The authors put it forward as true.
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A result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“At 12 months of age, pioglitazone treatment elevated the expression of all M2 markers assayed, Fizz1, Ym1, and Arg1.”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Results > Pioglitazone suppresses glial activation and enhances amyloid clearance
plaindirect- Asserted: passes — The authors put it forward as true.
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A result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“Six-month-old PPARγ CKO animals displayed more than a 50% reduction in cortical protein levels of abca1 and apoe, demonstrating the requirement for PPARγ in LXR actions in the brain and the normal expression of its target genes apoe and abca1 (Fig. 2G,H).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Results > PPARγ facilitates the degradation of soluble Aβ through an LXR/ApoE-dependent pathway
boosteddirect- Asserted: passes — The authors put it forward as true.
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A result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“In 12 month animals, CD45+ microglia were associated with all amyloid deposits regardless of treatment (Fig. 6M–R).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Results > Pioglitazone suppresses glial activation and enhances amyloid clearance
plaincorrelational- Asserted: passes — The authors put it forward as true.
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A result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“However, only ~50% of 6E10-positive deposits were associated with CD45+ microglia in pioglitazone-treated animals (data not shown).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Results > Pioglitazone suppresses glial activation and enhances amyloid clearance
plaincorrelational- Asserted: passes — The authors put it forward as true.
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- Load-bearing: unclear — It is unclear whether the paper’s conclusions depend on it.
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A result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“Importantly, pioglitazone treatment of primary microglia and astrocytes resulted in the induction of the LXR target genes, abca1 and apoe in both cell types (Fig. 1B–F).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Results > PPARγ facilitates the degradation of soluble Aβ through an LXR/ApoE-dependent pathway
plaindirect- Asserted: passes — The authors put it forward as true.
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A result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“Both PPARγ and LXR antagonist treatment of microglia and astrocytes inhibited PPARγ-facilitated degradation of sAβ, suggesting that the activation of both PPAR and LXR pathways”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Results > PPARγ facilitates the degradation of soluble Aβ through an LXR/ApoE-dependent pathway
plaindirect- Asserted: passes — The authors put it forward as true.
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A result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“Fizz1 levels remained reduced in 12-month-old APP/PS1 animals (Fig. 7F).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Results > Pioglitazone suppresses glial activation and enhances amyloid clearance
plain- Asserted: passes — The authors put it forward as true.
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A result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“Six-month-old APP/PS1 animals showed no differences in levels of Ym1 or Arg1 compared with WT animals.”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Results > Pioglitazone suppresses glial activation and enhances amyloid clearance
plain- Asserted: passes — The authors put it forward as true.
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A result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“Addition of ApoE or ApoA1 to pioglitazone-treated microglia or astrocytes (wild-type or ApoE−/−) did not have additive or synergistic effects on intracelluar sAβ degradation (Fig. 2E,F).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Results > PPARγ facilitates the degradation of soluble Aβ through an LXR/ApoE-dependent pathway
plain- Asserted: passes — The authors put it forward as true.
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A result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“Pioglitazone treatment of 6 and 12 month animals resulted in a significant induction in cortical protein levels of both ABCA1 and ApoE (Fig. 3A–D).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Results > PPARγ activation rapidly stimulates the clearance of Aβ in a mouse model of AD
plaindirect- Asserted: passes — The authors put it forward as true.
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A result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“Immunohistochemical analysis of 6E10-immunoreactive plaques in cortical slices from animals of both ages revealed ~40% and 30% (6 and 12 months, respectively) reduction in total levels of deposited Aβ compared with vehicle-treated APP/PS1 littermates (Fig. 4A–E).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Results > PPARγ activation rapidly stimulates the clearance of Aβ in a mouse model of AD
plaindirect- Asserted: passes — The authors put it forward as true.
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A result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“The association of Iba1+ microglial cells with amyloid plaques was reduced at both 6 and 12 months of age after pioglitazone treatment (Fig. 6D–F,J–L).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Results > Pioglitazone suppresses glial activation and enhances amyloid clearance
plaindirect- Asserted: passes — The authors put it forward as true.
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- Load-bearing: passes — The paper’s conclusions depend on it.
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A result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“dramatic reductions in brain levels of soluble and insoluble Aβ levels which correlated with the loss of both diffuse and dense-core plaques within the cortex.”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Abstract
plaincorrelational- Asserted: passes — The authors put it forward as true.
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A result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“In 6-month-old vehicle-treated animals, CD45 immunoreactivity was seen surrounding all 6E10-positive amyloid deposits (data not shown).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Results > Pioglitazone suppresses glial activation and enhances amyloid clearance
plaincorrelational- Asserted: passes — The authors put it forward as true.
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- Load-bearing: unclear — It is unclear whether the paper’s conclusions depend on it.
- Based: passes — The paper gives a reason why it holds.
A result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“Treatment of primary microglia or astrocytes with the synthetic agonist pioglitazone stimulated the intracellular degradation of soluble Aβ42 in a dose-dependent manner (Fig. 1A,D).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Results > PPARγ facilitates the degradation of soluble Aβ through an LXR/ApoE-dependent pathway
plaindirect- Asserted: passes — The authors put it forward as true.
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- Load-bearing: passes — The paper’s conclusions depend on it.
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A result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“There was a parallel loss in the number of thioflavin S+ dense-core amyloid plaques, with an ~40% and 30% decrease in the number of thioS+ plaques in 6 and 12 month animals, respectively (Fig. 4F).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Results > PPARγ activation rapidly stimulates the clearance of Aβ in a mouse model of AD
plaincorrelational- Asserted: passes — The authors put it forward as true.
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A result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“Additionally, the assessment of LXR target genes by quantitative real-time PCR showed a statistically significant increase in levels of mRNA for abca1, apoE, and lxrα (Fig. 3E,F).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Results > PPARγ activation rapidly stimulates the clearance of Aβ in a mouse model of AD
plaindirect- Asserted: passes — The authors put it forward as true.
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- Load-bearing: passes — The paper’s conclusions depend on it.
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A result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“Pioglitazone treatment resulted in the phenotypic polarization of microglial cells from a proinflammatory M1 state, into an anti-inflammatory M2 state that was associated with enhanced phagocytosis of deposited forms of amyloid.”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Abstract
plaindirect- Asserted: passes — The authors put it forward as true.
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A result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“This was restored by the addition of exogenously supplied ApoE (a mixture of all human ApoE isoforms) or human apolipoprotein A1 (ApoA1) (Fig. 2C,D).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Results > PPARγ facilitates the degradation of soluble Aβ through an LXR/ApoE-dependent pathway
plaindirect- Asserted: passes — The authors put it forward as true.
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- Load-bearing: passes — The paper’s conclusions depend on it.
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A result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“However, a trend for a reduction in cd45 mRNA levels was observed in pioglitazone-treated animals of both age groups (Fig. 6S,T).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Results > Pioglitazone suppresses glial activation and enhances amyloid clearance
hedged- Asserted: passes — The authors put it forward as true.
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- Load-bearing: unclear — It is unclear whether the paper’s conclusions depend on it.
- Based: passes — The paper gives a reason why it holds.
A result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“Pioglitazone treatment significantly reduced the levels of GFAP-immunoreactive astrocytes surrounding amyloid plaques in transgenic animals at both 6 and 12 months of age and seen by immunohistochemistry (Fig. 5A–L).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Results > Pioglitazone suppresses glial activation and enhances amyloid clearance
plaindirect- Asserted: passes — The authors put it forward as true.
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- Load-bearing: passes — The paper’s conclusions depend on it.
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A result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“Brain levels of the inflammatory cytokines, Il-1β and tnfα, were also reduced in 12- but not 6-month-old treated animals, verifying the anti-inflammatory effects of PPARγ activation after amyloid deposition (Fig. 7E,F).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Results > Pioglitazone suppresses glial activation and enhances amyloid clearance
plaindirect- Asserted: passes — The authors put it forward as true.
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- Load-bearing: passes — The paper’s conclusions depend on it.
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A result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“We monitored mRNA levels of IDE and neprilysin to determine whether pioglitazone treatment enhanced expression of these proteases and observed no change (data not shown).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Results > PPARγ activation rapidly stimulates the clearance of Aβ in a mouse model of AD
plain- Asserted: passes — The authors put it forward as true.
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A result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“Pioglitazone-treated 12 month animals additionally showed an increase in levels of cortical pparγ mRNA (Fig. 3F).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Results > PPARγ activation rapidly stimulates the clearance of Aβ in a mouse model of AD
plaindirect- Asserted: passes — The authors put it forward as true.
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- Load-bearing: passes — The paper’s conclusions depend on it.
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A result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“We never detected Aβ-positive astrocytes in vehicle-treated animals (data not shown).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Results > Pioglitazone suppresses glial activation and enhances amyloid clearance
plain- Asserted: passes — The authors put it forward as true.
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- Load-bearing: passes — The paper’s conclusions depend on it.
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A result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“insoluble levels of both Aβ40 and Aβ42 were reduced by 50% and 40%, respectively (Fig. 4K,L).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Results > PPARγ activation rapidly stimulates the clearance of Aβ in a mouse model of AD
plaindirect- Asserted: passes — The authors put it forward as true.
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In the paper
“We report that astrocytes have the capacity to take up sAβ and degrade it in an apoE-dependent manner, similar to microglia (Figs. 1, 2; Jiang et al., 2008).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Discussion
What the sources say
- Not checked — no full text of the sourcecites Jiang et al., 2008 source
plaindirect- Asserted: passes — The authors put it forward as true.
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A result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“Amyloid-laden astrocytes were only found in the cortex of pioglitazone-treated animals and not in vehicle-treated APP/PS1 mice (Fig. 5).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Discussion
plain- Asserted: passes — The authors put it forward as true.
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A result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“Treatment of APP/PS1 mice with pioglitazone resulted in significant behavioral improvement that was not different from wild-type animals.”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Results > PPARγ activation ameliorates Aβ-related behavioral deficits
plaindirect- Asserted: passes — The authors put it forward as true.
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- Load-bearing: passes — The paper’s conclusions depend on it.
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A result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“Additionally, we monitored an increase in levels of Tgf-β in 12-month-old, but not 6-month-old, APP/PS1 mice.”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Results > Pioglitazone suppresses glial activation and enhances amyloid clearance
plain- Asserted: passes — The authors put it forward as true.
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- Load-bearing: unclear — It is unclear whether the paper’s conclusions depend on it.
- Based: passes — The paper gives a reason why it holds.
A result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“Animals were trained for four consecutive training sessions and all groups exhibited learning (Fig. 8B).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Results > PPARγ activation ameliorates Aβ-related behavioral deficits
plain- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: unclear — It is unclear whether the paper’s conclusions depend on it.
- Based: passes — The paper gives a reason why it holds.
A result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“One striking finding in this study was the extraordinary ability of pioglitazone to stimulate microglia to phagocytose amyloid deposits from the transgenic mice (Fig. 7).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Discussion
boosteddirect- Asserted: passes — The authors put it forward as true.
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- Load-bearing: passes — The paper’s conclusions depend on it.
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A result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“Importantly, these dramatic changes were elicited after a short 9 d period of drug treatment.”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Discussion
plaindirect- Asserted: passes — The authors put it forward as true.
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- Load-bearing: passes — The paper’s conclusions depend on it.
- Based: passes — The paper gives a reason why it holds.
In the paper
“Additionally, pioglitazone treatment reversed noncognitive behavioral deficits seen in APP/PS1 animals and restored distance and speed traveled to levels comparable to wild-type animals in an open field (Lalonde et al., 2005).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Results > PPARγ activation ameliorates Aβ-related behavioral deficits
What the sources say
- Not checked — no full text of the sourcecites Lalonde et al., 2005 source
plaindirect- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: unclear — It is unclear whether the paper’s conclusions depend on it.
- Based: passes — The paper gives a reason why it holds.
In the paper
“Additionally, pioglitazone treatment reversed noncognitive behavioral deficits seen in APP/PS1 animals and restored distance and speed traveled to levels comparable to wild-type animals in an open field (Lalonde et al., 2005).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Results > PPARγ activation ameliorates Aβ-related behavioral deficits
What the sources say
- Not checked — no full text of the sourcecites Lalonde et al., 2005 source
plaindirect- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: unclear — It is unclear whether the paper’s conclusions depend on it.
- Based: passes — The paper gives a reason why it holds.
A result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“However, vehicle-treated APP/PS1 animals showed significant impairments in memory retention as evidenced by the low number of freezes in the cued environment (Fig. 8A).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Results > PPARγ activation ameliorates Aβ-related behavioral deficits
plaincorrelational- Asserted: passes — The authors put it forward as true.
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- Load-bearing: passes — The paper’s conclusions depend on it.
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A result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“The principal conceptual advance in this study was the recognition that in astrocytes and microglia the expression of apoe is robustly regulated through a coupled metabolic pathway regulated by PPARγ and LXRα, and genetic inactivation of PPARγ in astrocytes results in a dramatic reduction in the expression of these LXR target genes (Fig. 2).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Discussion
plaindirect- Asserted: passes — The authors put it forward as true.
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- Load-bearing: passes — The paper’s conclusions depend on it.
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A result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“The principal conceptual advance in this study was the recognition that in astrocytes and microglia the expression of apoe is robustly regulated through a coupled metabolic pathway regulated by PPARγ and LXRα, and genetic inactivation of PPARγ in astrocytes results in a dramatic reduction in the expression of these LXR target genes (Fig. 2).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Discussion
boosteddirect- Asserted: passes — The authors put it forward as true.
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- Load-bearing: passes — The paper’s conclusions depend on it.
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In the paper
“Importantly, apoE-containing HDL particles facilitate the proteolytic degradation of soluble Aβ (sAβ) (Jiang et al., 2008).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Introduction
What the sources say
- Not checked — no full text of the sourcecites Jiang et al., 2008 source
plaindirect- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: passes — The paper’s conclusions depend on it.
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A conclusion the paper draws from its own results — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“suggesting that PPARγ activation does not alter the expression or processing of APP (Fig. 4I,J,M,N).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Results > PPARγ activation rapidly stimulates the clearance of Aβ in a mouse model of AD
hedged- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: passes — The paper’s conclusions depend on it.
- Based: passes — The paper gives a reason why it holds.
A conclusion the paper draws from its own results — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“These data document a robust PPARγ-LXR linkage in the brain which has not previously been recognized.”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Results > PPARγ facilitates the degradation of soluble Aβ through an LXR/ApoE-dependent pathway
boostedcorrelational- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: passes — The paper’s conclusions depend on it.
- Based: passes — The paper gives a reason why it holds.
A conclusion the paper draws from its own results — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“supports the therapeutic utility of PPARγ agonists for the treatment of Alzheimer's disease.”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Abstract
plaindirect- Asserted: passes — The authors put it forward as true.
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A conclusion the paper draws from its own results — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“These data suggest that in addition to the clearance of soluble forms of Aβ, pioglitazone treatment facilitated the removal of both diffuse and dense-core Aβ deposits by microglia and astrocytes, presumably through engaging the phagocytic machinery of these cells.”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Results > Pioglitazone suppresses glial activation and enhances amyloid clearance
hedgeddirect- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: passes — The paper’s conclusions depend on it.
- Based: passes — The paper gives a reason why it holds.
A conclusion the paper draws from its own results — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“We argue that sAβ clearance results from stimulation of apoE-dependent proteolysis of Aβ, whereas deposited Aβ is removed phagocytically by microglia as a result of a PPARγ-dependent alternative M2 polarization of microglia.”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Introduction
plaindirect- Asserted: passes — The authors put it forward as true.
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- Load-bearing: passes — The paper’s conclusions depend on it.
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A conclusion the paper draws from its own results — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“These data provide a mechanistic explanation for how PPARγ activation facilitates amyloid clearance and supports the therapeutic utility of PPARγ agonists”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Abstract
plaindirect- Asserted: passes — The authors put it forward as true.
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A conclusion the paper draws from its own results — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“These data support the view that PPARγ activation promotes the clearance of insoluble Aβ by astrocytes.”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Results > Pioglitazone suppresses glial activation and enhances amyloid clearance
plaindirect- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: passes — The paper’s conclusions depend on it.
- Based: passes — The paper gives a reason why it holds.
A conclusion the paper draws from its own results — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“These data suggest that the changes seen in amyloid deposition were indeed a result of Aβ catabolism and not due to alterations in APP processing or changes in levels of Aβ-degrading enzymes.”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Results > PPARγ activation rapidly stimulates the clearance of Aβ in a mouse model of AD
hedgeddirect- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: passes — The paper’s conclusions depend on it.
- Based: passes — The paper gives a reason why it holds.
A conclusion the paper draws from its own results — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“The reduction in levels of GFAP, CD45, and Iba1 in pioglitazone-treated animals may be attributed to the role of PPARγ in suppression of the inflammatory response.”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Results > Pioglitazone suppresses glial activation and enhances amyloid clearance
hedgedconditional- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: passes — The paper’s conclusions depend on it.
- Based: passes — The paper gives a reason why it holds.
A conclusion the paper draws from its own results — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“Strikingly, a brief 9 d pioglitazone treatment of APP/PS1 mice reversed pathological and behavioral phenotypes in treated mice.”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Introduction
boosteddirect- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: passes — The paper’s conclusions depend on it.
- Based: passes — The paper gives a reason why it holds.
A conclusion the paper draws from its own results — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“verifying the anti-inflammatory effects of PPARγ activation after amyloid deposition (Fig. 7E,F).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Results > Pioglitazone suppresses glial activation and enhances amyloid clearance
boosteddirect- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: passes — The paper’s conclusions depend on it.
- Based: passes — The paper gives a reason why it holds.
A conclusion the paper draws from its own results — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“whereas deposited Aβ is removed phagocytically by microglia as a result of a PPARγ-dependent alternative M2 polarization of microglia.”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Introduction
plaindirect- Asserted: passes — The authors put it forward as true.
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- Load-bearing: passes — The paper’s conclusions depend on it.
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A conclusion the paper draws from its own results — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“suggesting that the activation of both PPAR and LXR pathways is necessary for the intracellular degradation of sAβ in these cell types (Fig. 2A,B).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Results > PPARγ facilitates the degradation of soluble Aβ through an LXR/ApoE-dependent pathway
hedgeddirect- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: passes — The paper’s conclusions depend on it.
- Based: passes — The paper gives a reason why it holds.
None of the sources cited for this claim could be matched to a paper in Crossref, so there is nothing to check it against.
In the paper
“Because of the efficacy of PPARγ agonist action in mouse models of AD, small clinical trials evaluated the effects of receptor activation and found that pioglitazone treatment improved memory and cognition in patients with mild to moderate AD (Hanyu et al., 2009; Sato et al., 2011).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Discussion
What the sources say
- Not matchedcites Hanyu et al., 2009
Couldn’t be matched to a paper in Crossref, so it can’t be checked.
- Not matchedcites Sato et al., 2011
Couldn’t be matched to a paper in Crossref, so it can’t be checked.
plaindirect- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: unclear — It is unclear whether the paper’s conclusions depend on it.
- Based: passes — The paper gives a reason why it holds.
- Not matched
None of the sources cited for this claim have been checked yet.
In the paper
“Several studies have shown that astrocytes are capable of taking up amyloid peptides (Funato et al., 1998; Matsunaga et al., 2003; Nagele et al., 2003; Lasagna-Reeves and Kayed, 2011), and in vitro this ability was restricted to adult astrocytes (al-Ali and al-Hussain, 1996; Wyss-Coray et al., 2003; Koistinaho et al., 2004; Pihlaja et al., 2008).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Discussion
What the sources say
- Not checked — no full text of the sourcecites Funato et al., 1998
- Not checked — no full text of the sourcecites Matsunaga et al., 2003 source
- Not matchedcites Nagele et al., 2003
Couldn’t be matched to a paper in Crossref, so it can’t be checked.
plaindirect- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: passes — The paper’s conclusions depend on it.
- Based: passes — The paper gives a reason why it holds.
A conclusion the paper draws from its own results — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“Moreover, we have identified a previously unappreciated role of astrocytes in the removal of amyloid from the brain.”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Discussion
boosteddirect- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: passes — The paper’s conclusions depend on it.
- Based: passes — The paper gives a reason why it holds.
A conclusion the paper draws from its own results — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“These results demonstrate that PPARγ activation results in the rapid improvement of memory in an AD mouse model.”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Results > PPARγ activation ameliorates Aβ-related behavioral deficits
boosteddirect- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: passes — The paper’s conclusions depend on it.
- Based: passes — The paper gives a reason why it holds.
In the paper
“A larger phase II clinical trial showed improvements in memory retention and attention with the treatment of rosiglitazone (6 months) in patients who did not possess an ApoE4 allele (Risner et al., 2006).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Discussion
What the sources say
- Not matchedcites Risner et al., 2006
Couldn’t be matched to a paper in Crossref, so it can’t be checked.
plaindirect- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: unclear — It is unclear whether the paper’s conclusions depend on it.
- Based: passes — The paper gives a reason why it holds.
- Not matched
In the paper
“PPARγ activation has been shown to enhance the phagocytic capabilities of peripheral M2 polarized macrophages (Chinetti-Gbaguidi et al., 2011).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Discussion
What the sources say
- Not checked — no full text of the sourcecites Chinetti-Gbaguidi et al., 2011 source
boosteddirect- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: unclear — It is unclear whether the paper’s conclusions depend on it.
- Based: passes — The paper gives a reason why it holds.
What the paper did — it cites no source for this, so there is none to check. The quote shows it is in the paper.
In the paper
“It was difficult to assess Aβ uptake by microglia in 6-month-old animals since these animals are at the earlier stages of amyloid deposition, and there are very few Iba1+ cells surrounding Aβ deposits in vehicle-treated animals and fewer still in pioglitazone-treated animals.”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Results > Pioglitazone suppresses glial activation and enhances amyloid clearance
plaindirect- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: unclear — It is unclear whether the paper’s conclusions depend on it.
- Based: passes — The paper gives a reason why it holds.
A conclusion the paper draws from its own results — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“This study strongly supports the view that PPARγ activation initiates amyloid clearance pathways in astrocytes and suggests a reevaluation for the role of astrocytes in amyloid clearance in AD.”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Discussion
boosteddirect- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: passes — The paper’s conclusions depend on it.
- Based: passes — The paper gives a reason why it holds.
- Rests on an assumption: Astrocytes greatly outnumber microglia in the CNS.
A conclusion the paper draws from its own results — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“We report that the clearance of soluble forms of Aβ occurs through the ability of PPARγ to induce the expression of apoe through which it promotes sAβ degradation.”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Discussion
plaindirect- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: passes — The paper’s conclusions depend on it.
- Based: passes — The paper gives a reason why it holds.
A conclusion the paper draws from its own results — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“suggesting that apoE does not play a role in the M2 polarization of microglial cells that is seen in vivo.”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Results > Pioglitazone suppresses glial activation and enhances amyloid clearance
hedged- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: passes — The paper’s conclusions depend on it.
- Based: passes — The paper gives a reason why it holds.
A conclusion the paper draws from its own results — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“We have documented the extraordinary efficacy of PPARγ activation in ameliorating the pathologic and behavioral deficits in an AD mouse model.”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Discussion
boosteddirect- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: passes — The paper’s conclusions depend on it.
- Based: passes — The paper gives a reason why it holds.
A conclusion the paper draws from its own results — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“A primary finding of this study is the demonstration that PPARγ may act through distinct mechanisms to clear soluble and deposited, fibrillar forms of Aβ.”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Discussion
hedgedconditional- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: passes — The paper’s conclusions depend on it.
- Based: passes — The paper gives a reason why it holds.
In the paper
“We have previously documented that apoE acts to facilitate the proteolysis of soluble Aβ peptides both in the interstitial fluid and intracellularly by microglia through the actions of IDE and neprilysin, respectively (Jiang et al., 2008).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Discussion
What the sources say
- Not checked — no full text of the sourcecites Jiang et al., 2008 source
boosteddirect- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: passes — The paper’s conclusions depend on it.
- Based: passes — The paper gives a reason why it holds.
A conclusion the paper draws from its own results — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“It is important to note that in these trials rosiglitazone was administered at dosages that were much lower than those needed to see beneficial effects on AD pathophysiology in rodent models of the disease.”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Discussion
plain- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: passes — The paper’s conclusions depend on it.
- Based: passes — The paper gives a reason why it holds.
A conclusion the paper draws from its own results — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“PPARγ activation changes the inflammatory milieu of the brain by phenotypically polarizing microglia to an alternative, M2 phenotype, allowing them to phagocytically remove amyloid deposits (Fig. 9).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Discussion
plaindirect- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: passes — The paper’s conclusions depend on it.
- Based: passes — The paper gives a reason why it holds.
- Rests on an assumption: Microglia are the principal immune effector cells in the CNS and the only professional phagocytes in the CNS.
- Rests on an assumption: In AD, microglia are competent phagocytes but fail to effectively clear deposited Aβ from the brain.
A conclusion the paper draws from its own results — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“Additionally, we have provided an explanation for how PPARγ modulates AD-related pathology in a mouse model.”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Discussion
boosteddirect- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: passes — The paper’s conclusions depend on it.
- Based: passes — The paper gives a reason why it holds.
- Probably not a substantial claim. 14
- It may state two separate claims as one. 25
The cited sources support only part of this claim — read each source’s verdict below.
In the paper
“apoE is synthesized mainly by astrocytes and plays an isoform-dependent role in modulating Aβ fibrillization and clearance (Holtzman, 2001; Zlokovic et al., 2005; Kim et al., 2009).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Introduction
What the sources say
- Not checked — no full text of the sourcecites Holtzman, 2001 source
- Could not verifycites Zlokovic et al., 2005 sourceExperimental — accuracy not yet measured
The matching passages are in the References section (page not provided): they list studies about apoE and Aβ metabolism or degradation, but do not state the claim as a finding of this paper. Searches for apoE, astrocytes, fibrillization, and apoE isoforms found no passage establishing that apoE is synthesized mainly by astrocytes or that its effects on Aβ fibrillization and clearance are isoform-dependent. (LLM, conf=0.72)
- Partly supportedcites Kim et al., 2009 sourceExperimental — accuracy not yet measured
The Physiological Function of ApoE section identifies astrocytes as the main source of apoE in the brain, and the Aβ Aggregation section reports isoform-dependent effects on fibrillization. The Aβ Clearance section reports an isoform-dependent difference in one mouse study, but also describes a study finding no difference and says whether isoform-specific clearance effects exist remains unresolved. The claim presents that clearance effect more definitively than the review does; page numbers were not available in our copy. (LLM, conf=0.84)
plaindirect- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: fails — The paper’s conclusions may not depend on it.
- Based: passes — The paper gives a reason why it holds.
Probably not a substantial claim. The paper’s conclusions may not depend on it. It may state two separate claims as one.
The cited sources support only part of this claim — read each source’s verdict below.
In the paper
“The nuclear receptors, peroxisome proliferator-activated receptor-γ (PPARγ) and Liver X receptors (LXRs) are ligand-activated transcription factors that act as fatty acid and cholesterol sensors (Forman et al., 1997; Kersten et al., 2000).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Introduction
What the sources say
- Partly supportedcites Forman et al., 1997 sourceExperimental — accuracy not yet measured
The Discussion says PPARγ responds to metabolic cues and appears to promote fatty-acid storage; the Results describe PPARs as nuclear fatty-acid and eicosanoid receptors. But the paper identifies PPARα, rather than PPARγ, as sensing broad changes in fatty-acid status, and the passages do not address LXRs as cholesterol sensors (searched: LXR, cholesterol sensors). (LLM, conf=0.85)
- Not checked — no full text of the sourcecites Kersten et al., 2000 source
plain- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: passes — The paper’s conclusions depend on it.
- Based: passes — The paper gives a reason why it holds.
Probably not a substantial claim. It may state two separate claims as one.
- Partly supported
The cited sources support only part of this claim — read each source’s verdict below.
In the paper
“Activation of these nuclear receptors suppresses NFκB-mediated inflammatory responses and activates their phagocytic machinery (Zelcer et al., 2007; Mukundan et al., 2009; Glass and Saijo, 2010).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Discussion
What the sources say
- Partly supportedcites Zelcer et al., 2007 sourceExperimental — accuracy not yet measured
The Results report that LXR activation suppresses inflammatory responses and restores fibrillar Aβ-stimulated phagocytosis in microglia when inflammation has inhibited it. The Discussion says this restoration occurs most likely through inhibition of NFκB activity. The claim overstates that proposed mechanism as established and describes restored phagocytosis as activation of phagocytic machinery. (LLM, conf=0.84)
- Partly supportedcites Mukundan et al., 2009 sourceExperimental — accuracy not yet measured
The Results section reports that PPAR-δ activation increases opsonin expression and apoptotic-cell uptake, and that PPAR-δ is required for the observed suppression of pro-inflammatory cytokines after apoptotic-cell feeding. The cited paper does not establish that this suppression is NFκB-mediated. Page numbers are unavailable in our copy. (LLM, conf=0.86)
- Not checked — no full text of the sourcecites Glass and Saijo, 2010 source
plaindirect- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: passes — The paper’s conclusions depend on it.
- Based: passes — The paper gives a reason why it holds.
It may state two separate claims as one.
- Partly supported
Every source the paper cites for this claim was checked and supports it.
In the paper
“While this pathway has been well documented in the periphery, it was not recognized to be operative in the brain until now (Chawla et al., 2001).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Discussion
What the sources say
- Supportedcites Chawla et al., 2001 source
The Introduction describes a PPARγ–LXRα pathway regulating cholesterol efflux in macrophages, and Figure 1 documents PPARγ-responsive LXRα expression in those cells. This supports the citation's point that the pathway was known outside the brain. The statement about its recognition in the brain is the citing authors' own framing, not a finding attributed to Chawla et al.; page numbers are unavailable in our copy. (LLM, conf=0.88)
boosted- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: passes — The paper’s conclusions depend on it.
- Based: passes — The paper gives a reason why it holds.
It may state two separate claims as one.
- Supported
Every source the paper cites for this claim was checked and supports it.
In the paper
“A number of studies of the thiazolidinedione agonists of PPARγ, pioglitazone (Actos) and rosiglitazone (Avandia), reported salutary effects of these drugs in murine models of AD (Mandrekar-Colucci and Landreth, 2011).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Discussion
What the sources say
- Supportedcites Mandrekar-Colucci and Landreth, 2011 source
Section 5 identifies pioglitazone (Actos) and rosiglitazone (Avandia) as thiazolidinedione PPARγ agonists and reviews multiple studies reporting benefits in AD mouse models. It also reports some null findings, but those do not negate the claim that a number of studies reported salutary effects. (LLM, conf=0.94)
plaindirect- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: fails — The paper’s conclusions may not depend on it.
- Based: passes — The paper gives a reason why it holds.
Probably not a substantial claim. The paper’s conclusions may not depend on it.
- Supported
Every source the paper cites for this claim was checked and supports it.
In the paper
“Recently, we have shown that activation of the Retinoid X Receptor, the obligate heterodimeric partner of PPARγ and LXRs, rapidly reverses AD pathology in mouse models of AD, and many of the beneficial effects seen in that study may occur through activation of PPAR signaling pathways (Cramer et al., 2012).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Discussion
What the sources say
- Supportedcites Cramer et al., 2012 source
The paper reports that the RXR agonist bexarotene rapidly reduced soluble Aβ and plaques and reversed behavioral deficits in AD mouse models (Abstract; passages 1, 5, 6, and 8). It also proposes activation of PPARγ:RXR as a mechanism and reports PPARγ-dependent Aβ degradation in treated cells (passage 4), consistent with the citing sentence’s hedged suggestion about PPAR signaling. (LLM, conf=0.92)
plaindirect- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: fails — The paper’s conclusions may not depend on it.
- Based: passes — The paper gives a reason why it holds.
Probably not a substantial claim. The paper’s conclusions may not depend on it.
- Supported
The sources with a verdict support this claim; the rest weren’t checked yet, have no full text we could read, or their verdicts aren’t rated yet.
In the paper
“Macrophages/microglia are capable of exhibiting a spectrum of phenotypic activation states which dictate their activities (Gordon, 2003; Mosser and Edwards, 2008; Mandrekar-Colucci and Landreth, 2010).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Discussion
What the sources say
- Not checked — no full text of the sourcecites Gordon, 2003 source
- Supportedcites Mosser and Edwards, 2008 source
The Abstract describes macrophages as plastic cells whose environmental responses produce populations with distinct functions and various ‘shades’ of activation. This supports the macrophage portion of the multiply cited sentence. (LLM, conf=0.91)
- Supportedcites Mandrekar-Colucci and Landreth, 2010 source
The ‘Alternative Activation of Microglial Cells’ section describes a continuum of macrophage activation states and says microglia can adopt a variety of activation states with different functions. It also distinguishes pro-inflammatory and tissue-repair activities associated with those states. The supplied passages have no page numbers. (LLM, conf=0.91)
plaindirect- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: passes — The paper’s conclusions depend on it.
- Based: passes — The paper gives a reason why it holds.
Probably not a substantial claim.
Every source the paper cites for this claim was checked and supports it.
In the paper
“Terwel et al. (2011) have recently reported similar findings upon activation of LXRs in a mouse model of AD.”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Discussion
What the sources say
- Supportedcites Terwel et al. (2011) source
In “Pharmacological treatment of APP23 mice with the LXR agonist TO901317,” Terwel et al. report activating LXRs in APP23 mice, a mouse model of AD. “Behavioral effects of TO901317 in AD models” reports reduced plaque load and a minor behavioral improvement in the present study. The citing sentence does not specify which findings were similar, but its attribution of findings following LXR activation in an AD mouse model is supported. (LLM, conf=0.88)
plaincorrelational- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: fails — The paper’s conclusions may not depend on it.
- Based: passes — The paper gives a reason why it holds.
Probably not a substantial claim. The paper’s conclusions may not depend on it.
- Supported
The cited sources support only part of this claim — read each source’s verdict below.
In the paper
“Indeed, PPARγ agonists have been shown to effectively lower both soluble levels of Aβ, reduce plaque burden, and improve behavior in some AD mouse models”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Discussion
What the sources say
- Partly supportedcites Yan et al., 2003 sourceExperimental — accuracy not yet measured
The Results report that pioglitazone significantly lowered SDS-soluble Aβ40 in Tg2576 mice, supporting the soluble-Aβ portion. The same Results say pioglitazone produced no significant change in plaque burden; the reported plaque reduction was with ibuprofen. The paper does not report behavioral improvement. (LLM, conf=0.88)
- Not matchedcites Camacho et al., 2004
Couldn’t be matched to a paper in Crossref, so it can’t be checked.
- Partly supportedcites Heneka et al., 2005 sourceExperimental — accuracy not yet measured
The Results section (page unavailable) reports that pioglitazone reduced soluble Aβ1–42 and Aβ1–42-positive plaque area in APPV717I mice. The paper does not report a behavioral improvement; its methods and results address drug treatment, inflammation, and amyloid measures, not behavioral testing. (LLM, conf=0.90)
boosteddirect- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: passes — The paper’s conclusions depend on it.
- Based: passes — The paper gives a reason why it holds.
It may state two separate claims as one.
- Partly supported
The cited sources support only part of this claim — read each source’s verdict below.
In the paper
“In the AD brain, microglia fail to effectively phagocytose Aβ and are also impaired in their ability to degrade Aβ fibrils (Chung et al., 1999; Cameron and Landreth, 2010).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Discussion
What the sources say
- Partly supportedcites Chung et al., 1999 sourceExperimental — accuracy not yet measured
The Abstract (page unavailable) reports that microglia took up fibrillar Aβ but degraded it only partly, then released intact Aβ. This supports poor degradation, not a failure to phagocytose Aβ. The study examined uptake and degradation by microglial cells; it does not establish that microglia in the AD brain are impaired in either ability. (LLM, conf=0.88)
- Not matchedcites Cameron and Landreth, 2010
Couldn’t be matched to a paper in Crossref, so it can’t be checked.
plain- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: passes — The paper’s conclusions depend on it.
- Based: passes — The paper gives a reason why it holds.
It may state two separate claims as one.
- Partly supported
A result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“We found that GFAP was elevated in APP/PS1 animals, but was significantly reduced in both age groups after pioglitazone treatment (Fig. 5N,O).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Results > Pioglitazone suppresses glial activation and enhances amyloid clearance
plaindirect- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: passes — The paper’s conclusions depend on it.
- Based: passes — The paper gives a reason why it holds.
It may state two separate claims as one.
A result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“The PPARγ conditional knock-out (PPARγ CKO) mice display significantly lower levels of pparγ mRNA which was reduced in the cortices of these animals by >70% (data not shown).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Results > PPARγ facilitates the degradation of soluble Aβ through an LXR/ApoE-dependent pathway
plaindirect- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: passes — The paper’s conclusions depend on it.
- Based: passes — The paper gives a reason why it holds.
It may state two separate claims as one.
A result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“mRNA levels of app, bace1, or psen1 or protein levels of APP and APP C-terminal fragments were not altered in response to drug treatment compared with vehicle-treated littermates, suggesting that PPARγ activation does not alter the expression or processing of APP (Fig. 4I,J,M,N).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Results > PPARγ activation rapidly stimulates the clearance of Aβ in a mouse model of AD
plain- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: passes — The paper’s conclusions depend on it.
- Based: passes — The paper gives a reason why it holds.
It may state two separate claims as one.
A result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“The mRNA levels for iba1 were significantly lower in pioglitazone-treated animals compared with vehicle-treated animals at 6 months of age and a trend toward a reduction was seen at 12 months of age (Fig. 6S,T).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Results > Pioglitazone suppresses glial activation and enhances amyloid clearance
plaindirect- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: passes — The paper’s conclusions depend on it.
- Based: passes — The paper gives a reason why it holds.
It may state two separate claims as one.
A result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“Microglia containing small amounts of Aβ were visualized in ~60% of microglial cells in vehicle-treated animals; however, the amount of Aβ internalized by microglia in vehicle-treated animals paled compared with microglia in the cortex of pioglitazone-treated animals (Fig. 7A–D)”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Results > Pioglitazone suppresses glial activation and enhances amyloid clearance
plaincorrelational- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: passes — The paper’s conclusions depend on it.
- Based: passes — The paper gives a reason why it holds.
It may state two separate claims as one.
A result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“In vehicle-treated animals, we observed astrocytes in close association with 6E10-positive amyloid plaques (Fig. 5C,I). However, after pioglitazone treatment, astrocytes were seen diffusely surrounding Aβ deposits and very little contact was observed between GFAP-positive cells and amyloid plaques (Fig. 5F,L).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Results > Pioglitazone suppresses glial activation and enhances amyloid clearance
plaincorrelational- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: passes — The paper’s conclusions depend on it.
- Based: passes — The paper gives a reason why it holds.
It may state two separate claims as one.
A result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“We demonstrate that PPARγ activation in the APP/PS1 animals provoked the conversion of at least a subset of microglia from a classical to alternative activation states, suppressing proinflammatory gene expression (IL-1β, TNFα) and inducing the expression of anti-inflammatory genes (YM1, Fizz1, Arg1, TGF-β) associated with tissue repair (Fig. 7).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Discussion
boosteddirect- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: passes — The paper’s conclusions depend on it.
- Based: passes — The paper gives a reason why it holds.
It may state two separate claims as one.
A result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“Moreover, pioglitazone treatment resulted in intracellular Aβ accumulation in astrocytes, coincident with the reduction in plaque burden (Fig. 5).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Discussion
plaindirect- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: passes — The paper’s conclusions depend on it.
- Based: passes — The paper gives a reason why it holds.
It may state two separate claims as one.
A result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“This latter function is accompanied by expression of genes necessary for phagocytosis and induction of phagocytic activity and this is consistent with our observation of enhanced phagocytosis of fAβ by microglia.”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Discussion
plaincorrelational- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: passes — The paper’s conclusions depend on it.
- Based: passes — The paper gives a reason why it holds.
It may state two separate claims as one.
A result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“Arg1 and TGF-β mRNA expression remained constant under all treatment paradigms while Ym1 or Fizz1 were not detected in the cultured microglia under any treatment condition (data not shown), suggesting that apoE does not play a role in the M2 polarization of microglial cells that is seen in vivo.”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Results > Pioglitazone suppresses glial activation and enhances amyloid clearance
plain- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: passes — The paper’s conclusions depend on it.
- Based: passes — The paper gives a reason why it holds.
It may state two separate claims as one.
A claim is checked only against the cited paper’s full text, never its abstract, and none is available for its sources.
In the paper
“Apolipoprotein E (apoE) is the principal genetic risk factor for sporadic, late-onset AD (Corder et al., 1993; Roses, 1996).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Introduction
What the sources say
plaincorrelational- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: fails — The paper’s conclusions may not depend on it.
- Based: passes — The paper gives a reason why it holds.
Probably not a substantial claim. The paper’s conclusions may not depend on it.
A conclusion the paper draws from its own results — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“This striking result suggests that short-term pioglitazone treatment not only reduces levels of sAβ but also results in the clearance of predeposited insoluble dense-core Aβ plaques in 12-month-old transgenic animals.”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Results > PPARγ activation rapidly stimulates the clearance of Aβ in a mouse model of AD
hedgeddirect- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: passes — The paper’s conclusions depend on it.
- Based: passes — The paper gives a reason why it holds.
It may state two separate claims as one.
A conclusion the paper draws from its own results — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“These experiments demonstrate that in vivo pioglitazone activates PPARγ and induces the expression of LXRα and its target genes in the AD brain, solidifying the linkage of a PPARγ-LXRα signaling pathway in the CNS.”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Results > PPARγ activation rapidly stimulates the clearance of Aβ in a mouse model of AD
boosteddirect- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: passes — The paper’s conclusions depend on it.
- Based: passes — The paper gives a reason why it holds.
It may state two separate claims as one.
A claim is checked only against the cited paper’s full text, never its abstract, and none is available for its sources.
In the paper
“LXR activation induces HDL particle formation through apoe and abca1 expression (Lehmann et al., 1997; Tall, 2008).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Introduction
What the sources say
plaindirect- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: passes — The paper’s conclusions depend on it.
- Based: passes — The paper gives a reason why it holds.
Probably not a substantial claim.
In the paper
“It should be noted that pioglitazone is poorly blood-brain barrier permeable, necessitating administration of the drug at 80 mg · kg−1 · d−1, a dose higher than previously administered (Maeshiba et al., 1997).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Results > PPARγ activation rapidly stimulates the clearance of Aβ in a mouse model of AD
What the sources say
- Not matchedcites Maeshiba et al., 1997
Couldn’t be matched to a paper in Crossref, so it can’t be checked.
plaindirect- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: unclear — It is unclear whether the paper’s conclusions depend on it.
- Based: passes — The paper gives a reason why it holds.
It may state two separate claims as one.
- Not matched
A conclusion the paper draws from its own results — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“We demonstrate that PPARγ activation induces lxrα, apoe, and abca1 expression, promoting Aβ clearance by both microglia and astrocytes.”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Introduction
boosteddirect- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: passes — The paper’s conclusions depend on it.
- Based: passes — The paper gives a reason why it holds.
It may state two separate claims as one.
A conclusion the paper draws from its own results — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“demonstrating the requirement for PPARγ in LXR actions in the brain and the normal expression of its target genes apoe and abca1 (Fig. 2G,H).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Results > PPARγ facilitates the degradation of soluble Aβ through an LXR/ApoE-dependent pathway
boosteddirect- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: passes — The paper’s conclusions depend on it.
- Based: passes — The paper gives a reason why it holds.
It may state two separate claims as one.
A claim is checked only against the cited paper’s full text, never its abstract, and none is available for its sources.
In the paper
“These cells undergo a classical M1 inflammatory activation in response of amyloid deposition, leading to a proinflammatory milieu in the brain (Wyss-Coray, 2006; Heneka et al., 2010).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Discussion
What the sources say
plaindirect- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: passes — The paper’s conclusions depend on it.
- Based: passes — The paper gives a reason why it holds.
It may state two separate claims as one.
What the paper did — it cites no source for this, so there is none to check. The quote shows it is in the paper.
In the paper
“The numbers of thioS+ plaques were counted by a blinded observer.”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Materials and Methods > Tissue collection and immunohistochemistry
plain- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: passes — The paper’s conclusions depend on it.
- Based: passes — The paper gives a reason why it holds.
Probably not a substantial claim.
None of the sources cited for this claim have been checked yet.
In the paper
“however, there has been no consensus on the mechanisms of action”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Discussion
What the sources say
- Not checked — no full text of the sourcecites Pedersen and Flynn, 2004 source
- Not matchedcites Escribano et al., 2010
Couldn’t be matched to a paper in Crossref, so it can’t be checked.
- Not matchedcites Toledo and Inestrosa, 2010
Couldn’t be matched to a paper in Crossref, so it can’t be checked.
plain- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: fails — The paper’s conclusions may not depend on it.
- Based: passes — The paper gives a reason why it holds.
Probably not a substantial claim. The paper’s conclusions may not depend on it.
What the paper did — it cites no source for this, so there is none to check. The quote shows it is in the paper.
In the paper
“We were unable to quantitatively evaluate this given the limitations of our astroglial marker, GFAP.”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Results > Pioglitazone suppresses glial activation and enhances amyloid clearance
plain- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: fails — The paper’s conclusions may not depend on it.
- Based: passes — The paper gives a reason why it holds.
Probably not a substantial claim. The paper’s conclusions may not depend on it.
In the paper
“In microglia, apoE facilitates soluble Aβ degradation through reduction of intracellular cholesterol levels and not through direct contact with the Aβ peptide (Lee et al., 2012).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Discussion
What the sources say
- Not matchedcites Lee et al., 2012
Couldn’t be matched to a paper in Crossref, so it can’t be checked.
plaindirect- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: fails — The paper’s conclusions may not depend on it.
- Based: passes — The paper gives a reason why it holds.
Probably not a substantial claim. The paper’s conclusions may not depend on it.
- Not matched
A conclusion the paper draws from its own results — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“These data suggest a conversion of microglial phenotype in APP/PS1 animals, from the classical M1 to an alternative M2 state following PPARγ activation, promoting the phagocytic removal of amyloid deposits by microglial cells.”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Results > Pioglitazone suppresses glial activation and enhances amyloid clearance
hedgeddirect- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: passes — The paper’s conclusions depend on it.
- Based: passes — The paper gives a reason why it holds.
It may state two separate claims as one.
A claim is checked only against the cited paper’s full text, never its abstract, and none is available for its sources.
In the paper
“Several studies have shown that astrocytes are capable of taking up amyloid peptides (Funato et al., 1998; Matsunaga et al., 2003; Nagele et al., 2003; Lasagna-Reeves and Kayed, 2011), and in vitro this ability was restricted to adult astrocytes (al-Ali and al-Hussain, 1996; Wyss-Coray et al., 2003; Koistinaho et al., 2004; Pihlaja et al., 2008).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Discussion
What the sources say
plain- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: fails — The paper’s conclusions may not depend on it.
- Based: passes — The paper gives a reason why it holds.
Probably not a substantial claim. The paper’s conclusions may not depend on it.
A conclusion the paper draws from its own results — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“In this paper we have demonstrated yet another mechanism through which PPARγ activation may ameliorate AD pathophysiology.”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Discussion
boostedconditional- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: passes — The paper’s conclusions depend on it.
- Based: passes — The paper gives a reason why it holds.
Probably not a substantial claim.
A conclusion the paper draws from its own results — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“We have shown a mechanistic linkage between the PPARγ and LXR pathways, which had not previously been documented in the brain, and results in the production of lipidated ApoE particles, facilitating the degradation of soluble Aβ species (Fig. 9).”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Discussion
boosteddirect- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: passes — The paper’s conclusions depend on it.
- Based: passes — The paper gives a reason why it holds.
It may state two separate claims as one.
A conclusion the paper draws from its own results — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“In this study we have demonstrated the rapid effects of PPARγ activation on amyloid clearance, microglial polarization, and the reversal of cognitive deficits associated with AD.”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Discussion
boosteddirect- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: passes — The paper’s conclusions depend on it.
- Based: passes — The paper gives a reason why it holds.
It may state two separate claims as one.
The paper relies on this without support.
The relationship between PPARγ and LXRs has not been explored in the brain.
“However, these relationships have yet to be explored in the brain.”
The paper relies on this without support.
Little is known about the mechanisms modulating the M2 phenotypic conversion in the AD brain.
“While M2 gene expression has been detected in the AD brain, little is known about the mechanisms modulating this phenotypic conversion (Colton et al., 2006; Jimenez et al., 2008; Maier et al., 2008).”
The paper relies on this without support.
APP/PS1 transgenic mice begin depositing fibrillar thioflavin S+ plaques at approximately 5–6 months of age.
“The APP/PS1 transgenic mice begin depositing fibrillar thioS+ plaques at ~5–6 months of age.”
The paper relies on this without support.
Robust amyloid phagocytosis in response to drug treatment, as seen in this study, has never been observed before.
“However, robust amyloid phagocytosis in response to drug treatment, as seen here, has to our knowledge never been observed before.”
The paper relies on this without support.
Astrocytes greatly outnumber microglia in the CNS.
“Astrocytes greatly outnumber microglia in the CNS, and their ability to remove even modest amounts of Aβ in the brain may have a significant impact on amyloid clearance in the brain.”
The paper relies on this without support.
Microglia are the principal immune effector cells in the CNS and the only professional phagocytes in the CNS.
“Microglia are the principal immune effector cells in the CNS and are the only professional phagocyte in the CNS.”
The paper relies on this without support.
In AD, microglia are competent phagocytes but fail to effectively clear deposited Aβ from the brain.
“An enigmatic feature of AD is that although microglia are competent phagocytes, they fail to effectively clear deposited Aβ from the brain.”
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The source backs part of the claim, not all of it — or backs it for a different population, condition or comparison.
In the citing paper
“Pioglitazone, which belongs to the TZD class of compounds, has been shown to reduce the amyloid load in an ApoE and/ or IDE-dependent process, neurofibrillary tangle formation and decline in cognitive function in 3xTgAD mice (Mandrekar-Colucci et al., 2012; Mansour et al., 2021; Searcy et al., 2012; Zhong et al., 2023).”
From Inhibition of the mitochondrial pyruvate carrier in astrocytes reduces amyloid and tau accumulation in the 3xTgAD mouse model of Alzheimer's disease (2024) · cites this paper as Mandrekar-Colucci et al., 2012
Experimental — accuracy not yet measuredThe cited source says what the claim attributes to it.
In the citing paper
“However, several studies have shown that defects in the phagocytic function of microglia can be restored with Aβ immunization [18, 23] and anti-inflammatory drug treatment [24, 25].”
From IL-1β-driven amyloid plaque clearance is associated with an expansion of transcriptionally reprogrammed microglia (2019) · cites this paper as [25]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
Each contradiction between two passages listed here was confirmed by an independent check.
Rated minor: it would not touch a result — a value no result rests on, a p-value that stays on the same side of the significance level, or a conflict that is clerical, resolved elsewhere in the paper, or outside its own findings.
In the paper
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Materials and Methods > Reagents
“The Aβ1–42 peptide was purchased from American Peptide Company, dissolved in DMSO to a final concentration of 1 μg/ml.”
§ Mechanisms Underlying the Rapid Peroxisome Proliferator-Activated Receptor-γ-Mediated Amyloid Clearance and Reversal of Cognitive Deficits in a Murine Model of Alzheimer's Disease > Materials and Methods > Aβ ELISAs
“Cells were then treated with 2 μg/ml soluble Aβ42 in serum-free medium for 24 h with the specified treatments.”
Citations
Cites
Corder, E. H., Saunders, A. M., Strittmatter, W. J., Schmechel, D. E., Gaskell, P. C., Small, G. W., Roses, A. D., Haines, J. L., & Pericak-Vance, M. A. (1993) · Science · doi:10.1126/science.8346443 · Cited by 9,429 · cited 2×
“Apolipoprotein E (apoE) is the principal genetic risk factor for sporadic, late-onset AD (Corder et al., 1993; Roses, 1996). apoE is synthesized mainly by astrocytes and plays an isoform-dependent role in modulating A fibrillization and clearance (Holtzman, 2001; Zlokovic et al., 2005; Kim et al.,…”
BackgroundExploring the full spectrum of macrophage activation
Mosser, D. M., & Edwards, J. P. (2008) · Nature Reviews Immunology · doi:10.1038/nri2448 · Cited by 9,144 · cited 4×
“mote tissue repair as well as phagocytosis (Gordon, 2003; Mantovani et al., 2004; Mosser and Edwards, 2008).”
BackgroundMethodInfluentialThe chemokine system in diverse forms of macrophage activation and polarization
MANTOVANI, A., SICA, A., SOZZANI, S., ALLAVENA, P., VECCHI, A., & LOCATI, M. (2004) · Trends in Immunology · doi:10.1016/j.it.2004.09.015 · Cited by 6,534 · cited 2×
“mote tissue repair as well as phagocytosis (Gordon, 2003; Mantovani et al., 2004; Mosser and Edwards, 2008).”
BackgroundMethodAlternative activation of macrophages
Gordon, S. (2003) · Nature Reviews Immunology · doi:10.1038/nri978 · Cited by 6,189 · cited 4×
“mote tissue repair as well as phagocytosis (Gordon, 2003; Mantovani et al., 2004; Mosser and Edwards, 2008).”
BackgroundMethodInfluentialQuerfurth, H. W., & LaFerla, F. M. (2010) · New England Journal of Medicine · doi:10.1056/nejmra0909142 · Cited by 4,569
Inflammation and Alzheimer’s disease
Akiyama, H. (2000) · Neurobiology of Aging · doi:10.1016/s0197-4580(00)00124-x · Cited by 4,514
Forman, B. M., Chen, J., & Evans, R. M. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.9.4312 · Cited by 2,116 · cited 2×
“The nuclear receptors, peroxisome proliferator-activated receptor- (PPAR ) and Liver X receptors (LXRs) are ligandactivated transcription factors that act as fatty acid and cholesterol sensors (Forman et al., 1997; Kersten et al., 2000).”
BackgroundMacrophage-specific PPARγ controls alternative activation and improves insulin resistance
Odegaard, J. I., Ricardo-Gonzalez, R. R., Goforth, M. H., Morel, C. R., Subramanian, V., Mukundan, L., Eagle, A. R., Vats, D., Brombacher, F., Ferrante, A. W., et al. (2007) · Nature · doi:10.1038/nature05894 · Cited by 2,107 · cited 1×
“…have only recently been appreciated to play an crucial role in promoting the phenotypic change of macrophages from a “classically” M1 activated to alternatively activated M2 states (Odegaard et al., 2007, 2008; Zelcer et al., 2007; Bouhlel et al., 2008; Kalinin et al., 2009; Chawla, 2010).”
BackgroundDecreased Clearance of CNS β-Amyloid in Alzheimer’s Disease
Mawuenyega, K. G., Sigurdson, W., Ovod, V., Munsell, L., Kasten, T., Morris, J. C., Yarasheski, K. E., & Bateman, R. J. (2010) · Science · doi:10.1126/science.1197623 · Cited by 2,078 · cited 2×
“Alzheimer's disease (AD) is characterized by the accumulation and deposition of amyloid β (Aβ) within the brain because of inefficient clearance of these peptides (Mawuenyega et al., 2010; Querfurth and LaFerla, 2010).”
BackgroundRoles of PPARs in health and disease
Kersten, S., Desvergne, B., & Wahli, W. (2000) · Nature · doi:10.1038/35013000 · Cited by 1,956 · cited 1×
“The nuclear receptors, peroxisome proliferator-activated receptor- (PPAR ) and Liver X receptors (LXRs) are ligandactivated transcription factors that act as fatty acid and cholesterol sensors (Forman et al., 1997; Kersten et al., 2000).”
Background
Cited by
Sorted by citations
Neuroinflammation and microglial activation in Alzheimer disease: where do we go from here?
Leng, F., & Edison, P. (2021) · Nature Reviews Neurology · doi:10.1038/s41582-020-00435-y · Cited by 3,179
Microglia Function in the Central Nervous System During Health and Neurodegeneration
Colonna, M., & Butovsky, O. (2017) · Annual Review of Immunology · doi:10.1146/annurev-immunol-051116-052358 · Cited by 2,894
Neuroinflammation and M2 Microglia: The Good, the Bad, and the Inflamed
Cherry, J. D., Olschowka, J. A., & O’Banion, M. K. (2014) · Journal of Neuroinflammation · doi:10.1186/1742-2094-11-98 · Cited by 1,748
“Several groups have observed that prolonged treatment with PPARγ agonists can reduce Alzheimer’s disease pathology, demonstrating its potential therapeutic efficacy [102,136,137].”
BackgroundInfluentialSleep and Alzheimer disease pathology--a bidirectional relationship
Ju, Y.-E. S., Lucey, B. P., & Holtzman, D. M. (2014) · Nature Reviews Neurology · doi:10.1038/nrneurol.2013.269 · Cited by 996
Innate immunity in Alzheimer's disease
Heneka, M. T., Golenbock, D. T., & Latz, E. (2015) · Nature Immunology · doi:10.1038/ni.3102 · Cited by 902
Metadata
- Authors
- Mandrekar-Colucci, S., Karlo, J. C., & Landreth, G. E.
- Affiliations
- Case Western Reserve University (US)
- Case Comprehensive Cancer Center
- ORCID
- Landreth, Gary E.
- Venue
- The Journal of Neuroscience
- Publisher
- Society for Neuroscience
- Published
- 2012-07-25
- Volume
- 32
- Issue
- 30
- Pages
- 10117-10128
- ISSN
- 0270-6474 (ISSN-L)
- 1529-2401
- PMC
- PMC3433721
- OpenAlex
- W2006505402
- Cited by
- 355
- References
- 99
- Citations by year
- 2026: 6 · 2025: 16 · 2024: 16 · 2023: 18 · 2022: 22 · 2021: 32 · 2020: 20 · 2019: 27 · 2018: 36 · 2017: 25 · 2016: 34 · 2015: 28 · 2014: 35 · 2013: 34 · 2012: 6
- FWCI
- 19.42
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Neuroscience (publishedVersion, cc-by-nc-sa)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (submittedVersion)
- PubMed Central (submittedVersion)
- Funders
- Case Western Reserve University
- National Institutes of Health (5r01ag030482-05, 5-R01-)
- National Institute on Aging (5-R01-AG030482, R01-AG030482)
- National Institute of Neurological Disorders and Stroke (F31NS061445)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Alzheimer's disease research and treatments
- Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Peroxisome Proliferator-Activated Receptors
- Keywords
- Alzheimer's disease, amyloid-beta clearance, PPARγ agonists, pioglitazone, liver X receptor, apolipoprotein E, microglial polarization, M2 microglia, astrocytes, amyloid plaques, cognitive impairment, APPswe/PS1ΔE9 mice, phagocytosis, anti-inflammatory therapy
- MeSH terms
- Liver X Receptors, Pioglitazone, Alzheimer Disease, Animals, Apolipoproteins E, Astrocytes, Brain, Cells, Cultured, Cognition, Disease Models, Animal, Peptide Fragments, Signal Transduction, Amyloid beta-Peptides, Microglia, Maze Learning, Thiazolidinediones, PPAR gamma, Mice, Orphan Nuclear Receptors
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
A language model drew the claims from the paper on 2026-10-20, under version 3 of the claim definition (model deepseek-v4.1-flash, prompt extract-v3).
A second model labelled each claim on 2026-10-20 (model jev-1.13-20260917, definition revision gold-guideline-v0.2).
Cited sources are checked with a language model’s help (gpt-6-sol).
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
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