Cited by 176DNA and Nucleic Acid ChemistryCarcinogens and Genotoxicity AssessmentMetal-Catalyzed Oxygenation Mechanisms

Singlet Oxygen Oxidation of Isolated and Cellular DNA: Product Formation and Mechanistic Insights

Cadet, Jean, Ravanat, Jean‐Luc, Martinez, Glaucia R., Medeiros, Marisa H. G., Mascio, Paolo Dl·2006·Photochemistry and Photobiology

Abstract

This survey focuses on recent aspects of the singlet oxygen oxidation of the guanine moiety of nucleosides, oligonucleotides, isolated and cellular DNA that has been shown to be the exclusive DNA target for this biologically relevant photo‐generated oxidant. A large body of mechanistic data is now available from studies performed on nucleosides in both aprotic solvents and aqueous solutions. A common process to both reaction conditions is the formation of 8‐oxo‐7,8‐dihydroguanine by reduction of 8‐hydroperoxyguanine that arises from the rearrangement of initially formed endoperoxide across the 4,8‐bond of the purine moiety. However, in organic solvent the hydroperoxide is converted as a major degradation pathway into a dioxirane that subsequently decomposes into a complex pattern of oxidation products. A different reaction that involved the formation of a highly reactive quinonoid intermediate consecutively to the loss of a water molecule from the 8‐hydroperoxide has been shown to occur in aqueous solution. Subsequent addition of a water molecule at C5 leads to the generation of a spiroiminodihydantoin compound via a rearrangement that involves an acyl shift. However, in both isolated and cellular DNA the latter decomposition pathway is at the best a minor process, because only 8‐oxo‐7,8‐dihydroguanine has been found to be generated. It is interesting to point out that singlet oxygen has been shown to contribute predominantly to the formation of 8‐oxo‐7,8‐dihydroguanine in the DNA of bacterial and human cells upon exposure to UVA radiation. It may be added that the formation of secondary singlet‐oxygen oxidation products of 8‐oxo‐7,8‐dihydroguanine, including spiroiminodihydantoin and oxaluric acid that were characterized in nucleosides and oligonucleotide, respectively, have not yet been found in cellular DNA.

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Crossref · 10.1562/2006-06-09-ir-914

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  • The endoperoxide intermediate rearranges quantitatively within double-stranded DNA into 8-hydroxyperoxyguanine, which is further reduced to 8-oxoGua, the exclusive singlet oxygen oxidation product.

    “The latter intermediate quantitatively rearranges within ds DNA into 8-hydroxyperoxyguanine (39,99) that is further reduced into 8-oxoGua (Fig. 5), the exclusive 1 O 2 oxidation product (39,100,101).”

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Authors
Cadet, J., Ravanat, J., Martinez, G. R., Medeiros, M. H. G., & Mascio, P. D.
Affiliations
  • Université Joseph Fourier (FR)
  • Universidade de São Paulo (BR)
Corresponding author
Cadet, Jean
Venue
Photochemistry and Photobiology
Publisher
Wiley
Published
2006-09
Volume
82
Issue
5
Pages
1219-1225
ISSN
  • 0031-8655 (ISSN-L)
  • 1751-1097
OpenAlex
W2175848502
Cited by
176
References
76
Citations by year
2026: 2 · 2025: 3 · 2024: 6 · 2023: 9 · 2022: 7 · 2021: 13 · 2020: 5 · 2019: 10 · 2018: 8 · 2017: 11 · 2016: 14 · 2015: 9 · 2014: 11 · 2013: 5 · 2012: 11
FWCI
0.85
Type
journal article
Language
en
Open access
closed
Locations
  • Photochemistry and Photobiology (publishedVersion)
  • PubMed (publishedVersion)
  • BioOne Complete (BioOne) (submittedVersion)
  • HAL (submittedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Carcinogens and Genotoxicity Assessment
  • Physical Sciences › Chemistry › Inorganic Chemistry › Metal-Catalyzed Oxygenation Mechanisms
Keywords
singlet oxygen, DNA oxidation, guanine, 8-oxo-7,8-dihydroguanine, dioxiranes, quinonoid intermediate, spiroiminodihydantoin, nucleosides, oligonucleotides, cellular DNA, UVA, reaction mechanism, solvent effects
MeSH terms
DNA, Guanine, Guanosine, Nucleosides, Oligonucleotides, Oxidation-Reduction, Solvents, Singlet Oxygen

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