Induction of 8‐Oxo‐7,8‐Dihydro‐2'‐Deoxyguanosine by Ultraviolet Radiation in Calf Thymus DNA and HeLa Cells
Abstract
Abstract— The levels of 8‐oxo‐7,8‐dihydro‐2'‐deoxyguanosine (8‐oxodGuo) in purified calf thymus DNA and HeLa cells were measured following exposure to either UVC, UVB or UVA wavelengths. This DNA damage was quantitated using HPLC coupled with an electrochemical detector. The 8‐oxodGuo was induced in purified DNA in a linear dose‐dependent fashion by each portion of the UV spectrum at yields of 100, 0.46 and 0.16 8‐oxodGuo per 10 5 2'‐deoxyguanosine (dGuo) per kJ/m 2 for UVC, UVB and UVA, respectively. However, the amount of 8‐oxodGuo in HeLa cells irradiated with these UV sources decreased to approximately 2.0, 0.013 and 0.0034 8‐oxodGuo per 10 5 dGuo per kJ/m 2, respectively. In contrast, the levels of cyclobutyl pyrimidine dimers were similar in both irradiated DNA and cells. Therefore, 8‐oxodGuo is induced in cells exposed to wavelengths throughout the UV spectrum although it appears that protective precesses exist within cells that reduce the UV‐induced formation of this oxidative DNA damage. Cell survival was also measured and the number of dimers or 8‐oxodGuo per genome per lethal event determined. These calculations are consistent with the conclusion that dimers play a major role in cell lethality for UVC‐ or UVB‐irradiated cells but only a minor role in cells exposed to UVA wavelengths. In addition, it was found that the relative yield of 8‐oxodGuo to dimers increased nearly 1000‐fold in both UVA‐irra‐diated cells and DNA compared with cells subjected to either UVC or UVB. These results are supportive of the hypothesis that 8‐oxodGuo, and possible other forms of oxidative damage, play an important role in the induction of biological effects caused by wavelengths in the UVA portion of the solar spectrum.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
UVA radiation has been shown since the 1970s to induce CPDs in bacteria, mammalian cells and human skin.
“In fact, UVA radiation has been shown since the 1970s, first in bacteria (29) and then in mammalian cells (32,55,(80)(81)(82)(83)(84) and human skin (61,65,(85)(86)(87), to be also able to induce CPDs.”
From Photoinduced Damage to Cellular DNA: Direct and Photosensitized Reactions<sup>†</sup> (2012) · cites this paper as [84]
Cites
Prooxidant States and Tumor Promotion
Cerutti, P. (1985) · Science · doi:10.1126/science.2981433 · Cited by 2,513 · see citing works
Insertion of specific bases during DNA synthesis past the oxidation-damaged base 8-oxodG
Shibutani, S., Takeshita, M., & Grollman, A. P. (1991) · Nature · doi:10.1038/349431a0 · Cited by 2,224 · see citing works
Kasai, H., Crain, P. F., Kuchino, Y., Nishimura, S., Ootsuyama, A., & Tanooka, H. (1986) · Carcinogenesis · doi:10.1093/carcin/7.11.1849 · Cited by 924 · see citing works
The role of 8-hydroxyguanine in carcinogenesis
Floyd, R. A. (1990) · Carcinogenesis · doi:10.1093/carcin/11.9.1447 · Cited by 721 · see citing works
Endogenous mutagens and the causes of aging and cancer
Ames, B. N., & Gold, L. S. (1991) · Mutation research. Fundamental and molecular mechanisms of mutagenesis · doi:10.1016/0027-5107(91)90157-j · Cited by 716 · see citing works
Oxidative mechanisms in carcinogenesis
Guyton, K. Z., & Kensler, T. W. (1993) · British Medical Bulletin · doi:10.1093/oxfordjournals.bmb.a072628 · Cited by 467 · see citing works
Sun exposure and non-melanocytic skin cancer
Kricker, A., Armstrong, B. K., & English, D. R. (1994) · Cancer Causes & Control · doi:10.1007/bf01804988 · Cited by 340 · see citing works
Moriya, M., Ou, C., Bodepudi, V., Johnson, F., Takeshita, M., & Grollman, A. P. (1991) · Mutation Research/DNA Repair · doi:10.1016/0921-8777(91)90067-y · Cited by 310 · see citing works
Kasai, H., Yamaizumi, Z., Berger, M., & Cadet, J. (1992) · Journal of the American Chemical Society · doi:10.1021/ja00050a078 · Cited by 300 · see citing works
Ito, K., Inoue, S., Yamamoto, K., & Kawanishi, S. (1993) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)38640-5 · Cited by 298 · see citing works
Mitchell, D. L., Haipek, C. A., & Clarkson, J. M. (1985) · Mutation Research Letters · doi:10.1016/s0165-7992(85)80018-x · Cited by 277 · see citing works
Self-citationUltraviolet radiation and immunology: something new under the sun--presidential address.
Kripke, M. L. (1994) · PubMed · Cited by 262 · see citing works
Absorption spectrum of DNA for wavelengths greater than 300 nm
Sutherland, J. C., & Griffin, K. P. (1981) · Radiation Research · doi:10.2307/3575456 · Cited by 214 · see citing works
Mitchell, D. L., Jen, J., & Cleaver, J. E. (1992) · Nucleic Acids Research · doi:10.1093/nar/20.2.225 · Cited by 205 · see citing works
Self-citationFloyd, R. A., Watson, J. J., Harris, J. A., et al. (1986) · Biochemical and Biophysical Research Communications · doi:10.1016/0006-291x(86)91156-3 · Cited by 191 · see citing works
A short synthesis of (+)-lycoricidine
Hudlický, T., & Olivo, H. F. (1992) · Journal of the American Chemical Society · doi:10.1021/ja00050a079 · Cited by 188 · see citing works
UV‐induced skin cancer in a hairless mouse model
de Gruijl, F. R., & Forbes, P. D. (1995) · BioEssays · doi:10.1002/bies.950170711 · Cited by 185 · see citing works
Piette, J. G. (1991) · Journal of Photochemistry and Photobiology B Biology · doi:10.1016/1011-1344(91)80030-l · Cited by 182 · see citing works
In vivo formation of oxidized DNA bases in tumor promoter-treated mouse skin.
Wei, H., & Frenkel, K. (1991) · PubMed · Cited by 181 · see citing works
Self-citationHydroxyl free radical mediated formation of 8-hydroxyguanine in isolated DNA
Floyd, R. A., West, M. S., Eneff, K. L., et al. (1988) · Archives of Biochemistry and Biophysics · doi:10.1016/0003-9861(88)90188-9 · Cited by 162 · see citing works
Wei, H., Cai, Q., & Rahn, R. O. (1996) · Carcinogenesis · doi:10.1093/carcin/17.1.73 · Cited by 158 · see citing works
Self-citationSolar-ultraviolet-induced damage to DNA.
Peak, M. J., & Peak, J. G. (1989) · PubMed · Cited by 134 · see citing works
Peak, M. J., Peak, J. G., Moehring, M. P., & Webs, R. B. (1984) · Photochemistry and Photobiology · doi:10.1111/j.1751-1097.1984.tb05349.x · Cited by 134 · see citing works
Beehler, B. C., Przybyszewski, J., Box, H. B., & Kulesz-Martin, M. F. (1992) · Carcinogenesis · doi:10.1093/carcin/13.11.2003 · Cited by 126 · see citing works
Ley, R. D. (1985) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.82.8.2409 · Cited by 112 · see citing works
Enninga, I. C., Groenendijk, R. T. L., Filon, A. R., van Zeeland, A. A., & Simons, J. W. I. M. (1986) · Carcinogenesis · doi:10.1093/carcin/7.11.1829 · Cited by 109 · see citing works
Pflaum, M., Boiteux, S., & Epe, B. (1994) · Carcinogenesis · doi:10.1093/carcin/15.2.297 · Cited by 94 · see citing works
The development of a radioimmunoassay for the detection of photoproducts in mammalian cell DNA
Mitchell, D. L., & Clarkson, J. M. (1981) · Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis · doi:10.1016/0005-2787(81)90066-6 · Cited by 77 · see citing works
Self-citationPeak, M. J., Peak, J. G., & Jones, C. (1985) · Photochemistry and Photobiology · doi:10.1111/j.1751-1097.1985.tb01552.x · Cited by 73 · see citing works
Mauthe, R. J., Cook, V. M., Coffing, S. L., & Baird, W. M. (1995) · Carcinogenesis · doi:10.1093/carcin/16.1.133 · Cited by 70 · see citing works
Rosen, J. E., Prahalad, A. K., & Williams, G. M. (1996) · Photochemistry and Photobiology · doi:10.1111/j.1751-1097.1996.tb02430.x · Cited by 68 · see citing works
Photosensitized formation of 8-hydroxydeoxyguanosine in cellular DNA by riboflavin
Yamamoto, F., Nishimura, S., & Kasai, H. (1992) · Biochemical and Biophysical Research Communications · doi:10.1016/0006-291x(92)91268-u · Cited by 65 · see citing works
Ley, R. D., & Applegate, L. A. (1985) · Journal of Investigative Dermatology · doi:10.1111/1523-1747.ep12276992 · Cited by 63 · see citing works
Rosenstein, B. S., Ducore, J. M., & Cummings, S. W. (1983) · Mutation Research/DNA Repair Reports · doi:10.1016/0167-8817(83)90032-9 · Cited by 52 · see citing works
Self-citationFischer‐Nielsen, A., Loft, S. H., & Jensen, K. G. (1993) · Carcinogenesis · doi:10.1093/carcin/14.11.2431 · Cited by 52 · see citing works
Mediation of 8-hydroxy-guanine formation in DNA by thiazin dyes plus light
Floyd, R. A., West, M. S., Eneff, K. L., & Schneider, J. E. (1990) · Free Radical Biology and Medicine · doi:10.1016/0891-5849(90)90097-3 · Cited by 49 · see citing works
UV-B and the immune system. A review with special emphasis on T cell-mediated immunity.
Goettsch, W., Garssen, J., Fr, D. G., et al. (1993) · PubMed · Cited by 40 · see citing works
PHOTOREACTIVATION OF ICR 2A FROG CELLS EXPOSED TO SOLAR UV WAVELENGTHS
Rosenstein, B. S. (1984) · Photochemistry and Photobiology · doi:10.1111/j.1751-1097.1984.tb04577.x · Cited by 37 · see citing works
Self-citationUltraviolet radiation, environmental health criteria 160
(1996) · Analytica Chimica Acta · doi:10.1016/s0003-2670(96)90545-0 · Cited by 28 · see citing works
Bech-Thomsen, N., & Wulf, H. C. (1995) · Photochemistry and Photobiology · doi:10.1111/j.1751-1097.1995.tb08729.x · Cited by 23 · see citing works
LOSS OF PHOTOREVERSIBILITY OF SUNBURN CELL INDUCTION IN Monodelphis domestica
Ley, R. D., & Applegate, L. A. (1987) · Photochemistry and Photobiology · doi:10.1111/j.1751-1097.1987.tb04773.x · Cited by 5 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Ultraviolet radiation-mediated damage to cellular DNA
Cadet, J., Sage, E., & Douki, T. (2005) · Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis · doi:10.1016/j.mrfmmm.2004.09.012 · Cited by 932 · see citing works
UV wavelength-dependent DNA damage and human non-melanoma and melanoma skin cancer
Pfeifer, G. P., & Besaratinia, A. (2012) · Photochemical & Photobiological Sciences · doi:10.1039/c1pp05144j · Cited by 473 · see citing works
Formation of UV-induced DNA damage contributing to skin cancer development
Cadet, J., & Douki, T. (2018) · Photochemical & Photobiological Sciences · doi:10.1039/c7pp00395a · Cited by 435 · see citing works
Cadet, J., Douki, T., & Ravanat, J. (2015) · Photochemistry and Photobiology · doi:10.1111/php.12368 · Cited by 355 · see citing works
Induction of oxidative DNA base damage in human skin cells by UV and near visible radiation
Kvam, E. (1997) · Carcinogenesis · doi:10.1093/carcin/18.12.2379 · Cited by 344 · see citing works
Photoinduced Damage to Cellular DNA: Direct and Photosensitized Reactions<sup>†</sup>
Cadet, J., Mouret, S., Ravanat, J., & Douki, T. (2012) · Photochemistry and Photobiology · doi:10.1111/j.1751-1097.2012.01200.x · Cited by 300 · see citing works
Distribution and Repair of Bipyrimidine Photoproducts in Solar UV-irradiated Mammalian Cells
Perdiz, D., Gróf, P., Mezzina, M., Nikaido, O., Moustacchi, E., & Sage, E. (2000) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)61437-7 · Cited by 248 · see citing works
Kuluncsics, Z., Perdiz, D., Brulay, E., Muel, B., & Sage, E. (1999) · Journal of Photochemistry and Photobiology B: Biology · doi:10.1016/s1011-1344(99)00034-2 · Cited by 239 · see citing works
Rochette, P. J. (2003) · Nucleic Acids Research · doi:10.1093/nar/gkg402 · Cited by 231 · see citing works
Mechanisms of UV-induced mutations and skin cancer
Pfeifer, G. P. (2020) · Genome Instability & Disease · doi:10.1007/s42764-020-00009-8 · Cited by 237 · see citing works
Sensitized formation of oxidatively generated damage to cellular DNA by UVA radiation
Cadet, J., Douki, T., Ravanat, J.-L., & Di Mascio, P. (2009) · Photochemical & Photobiological Sciences · doi:10.1039/b905343n · Cited by 213 · see citing works
Singlet Oxygen Oxidation of Isolated and Cellular DNA: Product Formation and Mechanistic Insights
Cadet, J., Ravanat, J., Martinez, G. R., Medeiros, M. H. G., & Mascio, P. D. (2006) · Photochemistry and Photobiology · doi:10.1562/2006-06-09-ir-914 · Cited by 176 · see citing works
Unravelling UVA-induced mutagenesis
Sage, E., Girard, P.-M., & Francesconi, S. (2012) · Photochemical & Photobiological Sciences · doi:10.1039/c1pp05219e · Cited by 167 · see citing works
Besaratinia, A., Synold, T. W., Chen, H.-H., Chang, C., Xi, B., Riggs, A. D., & Pfeifer, G. P. (2005) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0502311102 · Cited by 156 · see citing works
UVA-induced cyclobutane pyrimidine dimers in DNA: a direct photochemical mechanism?
Mouret, S., Philippe, C., Gracia-Chantegrel, J., Banyasz, A., Karpati, S., Markovitsi, D., & Douki, T. (2010) · Organic & Biomolecular Chemistry · doi:10.1039/b924712b · Cited by 149 · see citing works
Kozmin, S., Slezak, G., Reynaud-Angelin, A., Elie, C., de Rycke, Y., Boiteux, S., & Sage, E. (2005) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0504497102 · Cited by 148 · see citing works
Courdavault, S., Baudouin, C., Charveron, M., Favier, A., Cadet, J., & Douki, T. (2004) · Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis · doi:10.1016/j.mrfmmm.2004.07.011 · Cited by 145 · see citing works
Oxidation of Guanine in Cellular DNA by Solar UV Radiation: Biological Role†
Douki, T., Perdiz, D., Grof, P., Kuluncsics, Z., Moustacchi, E., Cadet, J., & Sage, E. (1999) · Photochemistry and Photobiology · doi:10.1111/j.1751-1097.1999.tb07988.x · Cited by 133 · see citing works
Besaratinia, A., Kim, S.-I., Bates, S. E., & Pfeifer, G. P. (2007) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0610534104 · Cited by 107 · see citing works
Delinasios, G. J., Karbaschi, M., Cooke, M. S., & Young, A. R. (2018) · Scientific Reports · doi:10.1038/s41598-017-18924-4 · Cited by 95 · see citing works
Separation-of-function mutants unravel the dual-reaction mode of human 8-oxoguanine DNA glycosylase
Dalhus, B., Forsbring, M., Helle, I. H., Vik, E. S., Forstrøm, R. J., Backe, P. H., Alseth, I., & Bjørås, M. (2011) · Structure · doi:10.1016/j.str.2010.09.023 · Cited by 89 · see citing works
- Authors
- Zhang, X., Rosenstein, B. S., Wang, Y., Lebwohl, M., Mitchell, D. M., & Wei, H.
- Affiliations
- City University of New York (US)
- The University of Texas System (US)
- Corresponding author
- Wei, Huachen
- Venue
- Photochemistry and Photobiology
- Publisher
- Wiley
- Published
- 1997-01
- Volume
- 65
- Issue
- 1
- Pages
- 119-124
- ISSN
- 0031-8655 (ISSN-L)
- 1751-1097
- OpenAlex
- W2007010947
- Cited by
- 129
- References
- 41
- Citations by year
- 2025: 1 · 2023: 4 · 2022: 2 · 2021: 3 · 2020: 3 · 2019: 2 · 2018: 3 · 2017: 2 · 2016: 1 · 2015: 6 · 2014: 3 · 2013: 1 · 2012: 1
- FWCI
- 3.91
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Photochemistry and Photobiology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (7r01ca060994-03, 5r01ca045078-08, 5r01ca061764-04)
- National Cancer Institute (CA61764, CA60994, CA 45078)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Carcinogens and Genotoxicity Assessment
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Effects of Radiation Exposure
- Keywords
- 8-oxo-7,8-dihydro-2'-deoxyguanosine, oxidative DNA damage, ultraviolet radiation, UVA, UV-B radiation, UV-C, calf thymus DNA, HeLa cells, cyclobutane pyrimidine dimers, cell survival, DNA repair
- MeSH terms
- 8-Hydroxy-2'-Deoxyguanosine, Animals, Cattle, Deoxyguanosine, DNA, HeLa Cells, Humans, Ultraviolet Rays
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
