Cited by 131Wound Healing and TreatmentsNanoparticle-Based Drug DeliveryNanoparticles: synthesis and applications

Nitric oxide-releasing poly(lactic-co-glycolic acid)-polyethylenimine nanoparticles for prolonged nitric oxide release, antibacterial efficacy, and in vivo wound healing activity

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Yoo, Jin-Wook, Nurhasni, Hasan, Cao, Jiafu, Choi, Moonjeong, Kim, Il, Lee, Bok Luel, Jung, Yunjin·2015·International Journal of Nanomedicine

Abstract

Nitric oxide (NO)-releasing nanoparticles (NPs) have emerged as a wound healing enhancer and a novel antibacterial agent that can circumvent antibiotic resistance. However, the NO release from NPs over extended periods of time is still inadequate for clinical application. In this study, we developed NO-releasing poly(lactic-co-glycolic acid)-polyethylenimine (PEI) NPs (NO/PPNPs) composed of poly(lactic-co-glycolic acid) and PEI/diazeniumdiolate (PEI/NONOate) for prolonged NO release, antibacterial efficacy, and wound healing activity. Successful preparation of PEI/NONOate was confirmed by proton nuclear magnetic resonance, Fourier transform infrared spectroscopy, and ultraviolet/visible spectrophotometry. NO/PPNPs were characterized by particle size, surface charge, and NO loading. The NO/PPNPs showed a prolonged NO release profile over 6 days without any burst release. The NO/PPNPs exhibited potent bactericidal efficacy against methicillin-resistant Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa concentration-dependently and showed the ability to bind on the surface of the bacteria. We also found that the NO released from the NO/PPNPs mediates bactericidal efficacy and is not toxic to healthy fibroblast cells. Furthermore, NO/PPNPs accelerated wound healing and epithelialization in a mouse model of a MRSA-infected wound. Therefore, our results suggest that the NO/PPNPs presented in this study could be a suitable approach for treating wounds and various skin infections.

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Authors
Yoo, J.-W., Nurhasni, H., Cao, J., Choi, M., Kim, I., Lee, B. L., & Jung, Y.
Affiliations
Pusan National University (KR)
Corresponding author
Yoo, Jin-Wook
Venue
International Journal of Nanomedicine
Published
2015-04
Volume
10
Pages
3065
ISSN
  • 1176-9114 (ISSN-L)
  • 1178-2013
OpenAlex
W2100509378
Cited by
131
References
43
Citations by year
2026: 1 · 2025: 3 · 2024: 8 · 2023: 3 · 2022: 12 · 2021: 24 · 2020: 17 · 2019: 19 · 2018: 17 · 2017: 16 · 2016: 10 · 2015: 1
FWCI
14.81
Type
journal article
Language
en
Open access
Locations
  • International Journal of Nanomedicine (publishedVersion, cc-by-nc)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by-nc)
  • PubMed Central (publishedVersion, cc-by-nc)
  • PubMed (publishedVersion)
  • Hasanuddin University Repository (submittedVersion)
Funders
  • National Research Foundation
  • National Research Foundation of Korea
Topics
  • Health Sciences › Medicine › Rehabilitation › Wound Healing and Treatments
  • Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
  • Physical Sciences › Materials Science › Materials Chemistry › Nanoparticles: synthesis and applications
Keywords
poly(lactic-co-glycolic acid), polyethyleneimine, diazeniumdiolates, antibacterial activity, methicillin-resistant Staphylococcus aureus, Pseudomonas aeruginosa, MRSA-infected wounds, wound healing, re-epithelialization, antibiotic resistance, skin infections, fibroblast cytotoxicity
MeSH terms
Polylactic Acid-Polyglycolic Acid Copolymer, Animals, Anti-Bacterial Agents, Bacteria, Cell Line, Nitric Oxide, Polyethyleneimine, Polyglycolic Acid, Wound Healing, Lactic Acid, Mice, Nanoparticles

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