Cited by 343Clinical Laboratory Practices and Quality ControlDementia and Cognitive Impairment ResearchAlzheimer's disease research and treatments

Recommendations to Standardize Preanalytical Confounding Factors in Alzheimer’s and Parkinson’s Disease Cerebrospinal Fluid Biomarkers: An Update

del Campo, Marta, Mollenhauer, Brit, Bertolotto, Antonio, Engelborghs, Sebastiaan, Hampel, Harald, Simonsen, Anja Hviid, Kapaki, Elisabeth, Kruse, Niels +12 more·2012·Biomarkers in Medicine

Abstract

Early diagnosis of neurodegenerative disorders such as Alzheimer's (AD) or Parkinson's disease (PD) is needed to slow down or halt the disease at the earliest stage. Cerebrospinal fluid (CSF) biomarkers can be a good tool for early diagnosis. However, their use in clinical practice is challenging due to the high variability found between centers in the concentrations of both AD CSF biomarkers (Aβ42, total tau and phosphorylated tau) and PD CSF biomarker (α-synuclein). Such a variability has been partially attributed to different preanalytical procedures between laboratories, thus highlighting the need to establish standardized operating procedures. Here, we merge two previous consensus guidelines for preanalytical confounding factors in order to achieve one exhaustive guideline updated with new evidence for Aβ42, total tau and phosphorylated tau, and α-synuclein. The proposed standardized operating procedures are applicable not only to novel CSF biomarkers in AD and PD, but also to biomarkers for other neurodegenerative disorders.

Integrity signals

Registry

Crossref · 10.2217/bmm.12.46

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  • Preanalytical aspects and development of standard operating procedures for sample handling and analysis, such as for AD biomarkers in CSF and blood, and quality control programs, are a requirement for in vitro diagnostic tests.

    “This especially applies to preanalytical aspects and the development of standard operating procedures for sample handling and analysis, such as for AD biomarkers in CSF and blood, 141 — 144 and to quality control programs, 145 which are a requirement for in vitro diagnostic tests.”

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Authors
del Campo, M., Mollenhauer, B., Bertolotto, A., Engelborghs, S., Hampel, H., Simonsen, A. H., Kapaki, E., Kruse, N., Le Bastard, N., Lehmann, S., Molinuevo, J. L., Parnetti, L., Perret-Liaudet, A., Sáez-Valero, J., Saka, E., Urbani, A., Vanmechelen, E., Verbeek, M., Visser, P. J., & Teunissen, C.
Affiliations
  • Department of Clinical Chemistry, Neurology Laboratory, VU University medical center (VUmc), Room PK1 Br016, De Boelelaan 1117, 1081 HV,Amsterdam,The Netherlands.
  • Universitätsmedizin Göttingen (DE)
  • Ospedale San Luigi Gonzaga (IT)
  • University of Antwerp (BE)
  • Goethe University Frankfurt (DE)
  • Copenhagen University Hospital (DK)
  • National and Kapodistrian University of Athens (GR)
  • Reference Center for Biological Markers of Dementia (BIODEM), Institute Born-Bunge, University of Antwerp, Antwerp, Belgium
  • Inserm (FR)
  • Alzheimer‘s Disease and Other Cognitive Disorders Unit, Neurology Service, Hospital Clinic, Barcelona, Spain
  • University of Perugia (IT)
  • Neurobiologie, CMRR, Gériatrie, Hospices Civils de Lyon, Université Lyon 1 – CNRS UMR5292 – INSERM - U1028, Lyon, France and Société Française de Biologie Clinique (SFBC), France
  • Instituto de Neurociencias de Alicante, Universidad Miguel Hernández-CSIC, Sant Joan d‘Alacant, Spain and Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas (CIBERNED), Spain
  • Hacettepe University Hospital (TR)
  • University of Rome Tor Vergata (IT)
  • ADx NeuroSciences (BE)
  • Radboud University Nijmegen (NL)
  • Maastricht University (NL)
  • Amsterdam UMC Location Vrije Universiteit Amsterdam (NL)
Venue
Biomarkers in Medicine
Published
2012-08
Volume
6
Issue
4
Pages
419-430
ISSN
  • 1752-0363 (ISSN-L)
  • 1752-0371
OpenAlex
W2139778458
Cited by
343
References
65
Citations by year
2026: 9 · 2025: 15 · 2024: 22 · 2023: 21 · 2022: 34 · 2021: 36 · 2020: 32 · 2019: 33 · 2018: 28 · 2017: 22 · 2016: 17 · 2015: 43 · 2014: 18 · 2013: 11
FWCI
2.51
Type
journal article
Language
en
Open access
closed
Locations
  • Biomarkers in Medicine (publishedVersion)
  • PubMed (publishedVersion)
  • Amsterdam UMC (VU Amsterdam) - Institutional Repository (publishedVersion)
  • Cineca Institutional Research Information System (Tor Vergata University) (submittedVersion)
  • Research Publications (Maastricht University) (submittedVersion)
  • DIGITAL.CSIC (Spanish National Research Council (CSIC)) (submittedVersion)
  • GRO (submittedVersion)
  • Pure Amsterdam UMC (submittedVersion)
  • Radboud Repository (Radboud University) (submittedVersion)
  • VUBIR (Vrije Universiteit Brussel) (submittedVersion)
  • Data Archiving and Networked Services (DANS) (submittedVersion)
Funders
Dutch Research Council
Topics
  • Health Sciences › Medicine › Physiology › Clinical Laboratory Practices and Quality Control
  • Health Sciences › Medicine › Psychiatry and Mental health › Dementia and Cognitive Impairment Research
  • Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
Keywords
cerebrospinal fluid biomarkers, Alzheimer's disease, Parkinson's disease, amyloid-beta 42, total tau, phosphorylated tau, alpha-synuclein, standard operating procedures, inter-laboratory variability, neurodegenerative diseases, early diagnosis
MeSH terms
Alzheimer Disease, Humans, Parkinson Disease, Peptide Fragments, Specimen Handling, Temperature, Biomarkers, Amyloid beta-Peptides, tau Proteins, Phase Transition, alpha-Synuclein

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