Alterations in ruminal bacterial populations at induction and recovery from diet-induced milk fat depression in dairy cows

Pitta, D.W.; Indugu, N.; Vecchiarelli, B.; Rico, D.E.; Harvatine, K.J.

doi:10.3168/jds.2016-12514

Cited by 74Ruminant Nutrition and Digestive PhysiologyMilk Quality and Mastitis in Dairy CowsAnimal Nutrition and Physiology

Alterations in ruminal bacterial populations at induction and recovery from diet-induced milk fat depression in dairy cows

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Pitta, D.W., Indugu, N., Vecchiarelli, B., Rico, D.E., Harvatine, K.J.·2018·Journal of Dairy Science

DOI 10.3168/jds.2016-12514

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Abstract

Ten ruminally cannulated Holstein cows were used in a crossover design that investigated changes in ruminal bacterial populations in response to induction and recovery from diet-induced milk fat depression (MFD). Further, the effect on the ruminal microbiota of the cows with diet-induced milk fat depression inoculated with rumen contents from non-milk fat-depressed donor cows was evaluated. Milk fat depression was induced during the first 10 d of each period by feeding a low-fiber, high-starch, and high-polyunsaturated fatty acid diet (26.1% neutral detergent fiber, 28.1% starch, 5.8% total fatty acids, and 1.9% C18:2), resulting in a 30% decrease in milk fat yield. Induction was followed by a recovery phase, where all cows were switched to a high-fiber, low-starch, and low-polyunsaturated fatty acid diet (31.8% neutral detergent fiber, 23% starch, 4.2% total fatty acids, and 1.2% C18:2) and were allocated to (1) control (no inoculation) or (2) ruminal inoculation with donor cow digesta (8 kg/d for 6 d). Ruminal samples were collected at the end of induction (d 10) and during recovery (d 13, 16, and 28), separated to solid and liquid fractions, extracted for DNA, PCR- amplified for the V1-V2 region of the 16S rRNA gene, and analyzed for bacterial diversity. Results indicated that bacterial communities were different between fractions. In each fraction, differences were significant between the induction (d 10) and recovery (d 13, 16, and 28) periods; however, differences were less apparent with time during the recovery period. The MFD (d 10) was typified by a reduction in the relative sequence abundance of Bacteroidetes and an increase in the relative sequence abundance of Firmicutes and Actinobacteria across both fractions. At the genus level, relative sequence abundance of unclassified Lachnospiraceae, Butyrivibrio, Bulleidia, and Coriobacteriaceae were higher on d 10 and were positively correlated with trans-10,cis-12 CLA and the trans-10 isomer, suggesting their potential role in altered biohydrogenation reactions. A switch to the recovery diet resulted in a sharp increase in the Bacteroidetes lineages and a decrease in Firmicutes members on d 13; however, this shift appears to stabilize by d 28, indicating the restoration process for ruminal bacteria from an altered state is gradual and complex. Inoculation of 10% of rumen contents from non-MFD donor cows to MFD cows revealed this procedure had transient effects on only a few bacterial populations, and such effects disappeared after d 16 following cessation of inoculation. It can be concluded that alterations in milk FA profiles at induction are preceded by microbial alterations in the rumen driven by dietary changes.

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The diet fed to ruminants is the primary determinant of bacterial community structure.
“The diet fed to ruminants is the primary determinant of bacterial community structure (95–97).”
From Phytogenic Additives Can Modulate Rumen Microbiome to Mediate Fermentation Kinetics and Methanogenesis Through Exploiting Diet–Microbe Interaction (2020) · cites this paper as [97]
The Introduction (page unavailable) says changes in dietary components can significantly alter rumen microbial communities, and the Discussion (page unavailable) reports that dietary modifications significantly altered bacterial community composition in the studied dairy cows. The paper does not establish that diet is the primary determinant relative to other factors, or that this ranking applies to ruminants generally. (LLM, conf=0.85)
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Additional file 5 of Assessment of fecal bacterial viability and diversity in fresh and frozen fecal microbiota transplant (FMT) product in horses
Long, A. E., Pitta, D., Hennessy, M. L., Indugu, N., Vecchiarelli, B., Luethy, D., Aceto, H. W., & Hurcombe, S. D. A. (2024) · Figshare · doi:10.6084/m9.figshare.26747492
Additional file 3 of Assessment of fecal bacterial viability and diversity in fresh and frozen fecal microbiota transplant (FMT) product in horses
Long, A. E., Pitta, D., Hennessy, M. L., Indugu, N., Vecchiarelli, B., Luethy, D., Aceto, H. W., & Hurcombe, S. D. A. (2024) · Figshare · doi:10.6084/m9.figshare.26747486
Additional file 11 of Assessment of fecal bacterial viability and diversity in fresh and frozen fecal microbiota transplant (FMT) product in horses
Long, A. E., Pitta, D., Hennessy, M. L., Indugu, N., Vecchiarelli, B., Luethy, D., Aceto, H. W., & Hurcombe, S. D. A. (2024) · Figshare · doi:10.6084/m9.figshare.26747510
Additional file 2 of Assessment of fecal bacterial viability and diversity in fresh and frozen fecal microbiota transplant (FMT) product in horses
Long, A. E., Pitta, D., Hennessy, M. L., Indugu, N., Vecchiarelli, B., Luethy, D., Aceto, H. W., & Hurcombe, S. D. A. (2024) · Figshare · doi:10.6084/m9.figshare.26241298
Additional file 10 of Assessment of fecal bacterial viability and diversity in fresh and frozen fecal microbiota transplant (FMT) product in horses
Long, A. E., Pitta, D., Hennessy, M. L., Indugu, N., Vecchiarelli, B., Luethy, D., Aceto, H. W., & Hurcombe, S. D. A. (2024) · Figshare · doi:10.6084/m9.figshare.26747507
Additional file 8 of Assessment of fecal bacterial viability and diversity in fresh and frozen fecal microbiota transplant (FMT) product in horses
Long, A. E., Pitta, D., Hennessy, M. L., Indugu, N., Vecchiarelli, B., Luethy, D., Aceto, H. W., & Hurcombe, S. D. A. (2024) · Figshare · doi:10.6084/m9.figshare.26747501
Additional file 12 of Assessment of fecal bacterial viability and diversity in fresh and frozen fecal microbiota transplant (FMT) product in horses
Long, A. E., Pitta, D., Hennessy, M. L., Indugu, N., Vecchiarelli, B., Luethy, D., Aceto, H. W., & Hurcombe, S. D. A. (2024) · Figshare · doi:10.6084/m9.figshare.26747513
Additional file 7 of Assessment of fecal bacterial viability and diversity in fresh and frozen fecal microbiota transplant (FMT) product in horses
Long, A. E., Pitta, D., Hennessy, M. L., Indugu, N., Vecchiarelli, B., Luethy, D., Aceto, H. W., & Hurcombe, S. D. A. (2024) · Figshare · doi:10.6084/m9.figshare.26747498
Additional file 4 of Assessment of fecal bacterial viability and diversity in fresh and frozen fecal microbiota transplant (FMT) product in horses
Long, A. E., Pitta, D., Hennessy, M. L., Indugu, N., Vecchiarelli, B., Luethy, D., Aceto, H. W., & Hurcombe, S. D. A. (2024) · Figshare · doi:10.6084/m9.figshare.26747489
Additional file 6 of Assessment of fecal bacterial viability and diversity in fresh and frozen fecal microbiota transplant (FMT) product in horses
Long, A. E., Pitta, D., Hennessy, M. L., Indugu, N., Vecchiarelli, B., Luethy, D., Aceto, H. W., & Hurcombe, S. D. A. (2024) · Figshare · doi:10.6084/m9.figshare.26747495
Additional file 9 of Assessment of fecal bacterial viability and diversity in fresh and frozen fecal microbiota transplant (FMT) product in horses
Long, A. E., Pitta, D., Hennessy, M. L., Indugu, N., Vecchiarelli, B., Luethy, D., Aceto, H. W., & Hurcombe, S. D. A. (2024) · Figshare · doi:10.6084/m9.figshare.26747504
Additional file 1 of Assessment of fecal bacterial viability and diversity in fresh and frozen fecal microbiota transplant (FMT) product in horses
Long, A. E., Pitta, D., Hennessy, M. L., Indugu, N., Vecchiarelli, B., Luethy, D., Aceto, H. W., & Hurcombe, S. D. A. (2024) · Figshare · doi:10.6084/m9.figshare.26241184
Bis-glycinate bound zinc supplementation in sows modulates sows and piglets gut microbiota and reduces piglet diarrhea incidence
Somboonna, N., Ruampatana, J., Kamolklang, P., Yamsrikaew, U., & Nuntapaitoon, M. (2026) · Scientific Reports · doi:10.1038/s41598-026-46189-3
Species-specific rumen microbial responses to dietary inhibition of methanogenesis in cows and goats
Corral‐Jara, K. F., Ramayo‐Caldas, Y., Bernard, L., Martin, C., Tournayre, J., Morgavi, D. P., & Popova, M. (2026) · Frontiers in Microbiology · doi:10.3389/fmicb.2026.1852812

Rapid changes in key ruminal microbial populations during the induction of and recovery from diet-induced milk fat depression in dairy cows
Rico, D. E., Preston, S. H., Risser, J. M., & Harvatine, K. J. (2015) · British Journal Of Nutrition · doi:10.1017/s0007114515001865 · Cited by 55 · see citing works
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Effect of 2-hydroxy-4-(methylthio) butanoate (HMTBa) supplementation on rumen bacterial populations in dairy cows when exposed to diets with risk for milk fat depression
Pitta, D., Indugu, N., Vecchiarelli, B., Hennessy, M. L., & Harvatine, K. (2019) · Figshare · doi:10.6084/m9.figshare.10058321
Shifts in bacterial community composition in the rumen of lactating dairy cows under milk fat-depressing conditions
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Authors: Pitta, D. W., Indugu, N., Vecchiarelli, B., Rico, D. E., & Harvatine, K. J.
Affiliations: University of Pennsylvania (US)
Centre de Recherche en Sciences Animales de Deschambault (CA)
Pennsylvania State University (US)
Corresponding author: Pitta, D.W.
ORCID: Pitta, D.W.
Indugu, N.
Vecchiarelli, B.
Rico, D.E.
Harvatine, K.J.
Venue: Journal of Dairy Science
Publisher: American Dairy Science Association
Published: 2018-01
Volume: 101
Issue: 1
Pages: 295-309
ISSN: 0022-0302 (ISSN-L)
DOI: 10.3168/jds.2016-12514
OpenAlex: W2765996011
Cited by: 73
References: 52
Citations by year: 2026: 3 · 2025: 6 · 2024: 19 · 2023: 6 · 2022: 5 · 2021: 6 · 2020: 12 · 2019: 11 · 2018: 5
FWCI: 13.30
Type: journal article
Language: en
Open access: bronze
doi.org
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations: Journal of Dairy Science (publishedVersion)
PubMed (publishedVersion)
Funders: University of Pennsylvania
Topics: Life Sciences › Agricultural and Biological Sciences › Agronomy and Crop Science › Ruminant Nutrition and Digestive Physiology
Life Sciences › Agricultural and Biological Sciences › Agronomy and Crop Science › Milk Quality and Mastitis in Dairy Cows
Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Nutrition and Physiology
Keywords: milk fat depression, rumen microbiota, dairy cattle, rumen inoculation, high-fiber diet, polyunsaturated fatty acids, trans-10, cis-12 CLA, biohydrogenation, Butyrivibrio, Lachnospiraceae, Bacteroidetes, Firmicutes
MeSH terms: Animals, Animal Feed, Animal Nutritional Physiological Phenomena, Bacteria, Cattle, Diet, Dietary Fiber, Female, Lactation, Milk, RNA, Ribosomal, 16S, Rumen

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Alterations in ruminal bacterial populations at induction and recovery from diet-induced milk fat depression in dairy cows

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