Cited by 94Alzheimer's disease research and treatmentsDementia and Cognitive Impairment ResearchRedox biology and oxidative stress

Redox proteomics analysis of brains from subjects with amnestic mild cognitive impairment compared to brains from subjects with preclinical Alzheimer’s disease: Insights into memory loss in MCI

Aluise, Christopher D., Robinson, Renã A.S., Cai, Jian, Pierce, William M., Markesbery, William R., Butterfield, D. Allan·2011·Journal of Alzheimer's Disease

Abstract

Alzheimer's disease (AD) is a central nervous system disorder pathologically characterized by senile plaques, neurofibrillary tangles, and synapse loss. A small percentage of individuals with normal antemortem psychometric scores, after adjustments for age and education, meet the neuropathological criteria for amnestic mild cognitive impairment (MCI) or AD; these individuals have been termed 'preclinical' or 'asymptomatic' AD (PCAD). In this study, we employed the immunochemical slot-blot method and two-dimensional gel-based redox proteomics to observe differences in protein levels and oxidative modifications between groups with equal levels of AD pathology who differ in regards to clinical symptoms of memory impairment. Results of global oxidative stress measurements revealed significantly higher levels of protein carbonyls in the MCI inferior parietal lobule (IPL) relative to PCAD (and controls), despite equal levels of neuropathology. Proteomics analysis of the IPL revealed differences in protein levels and specific carbonylation that are consistent with preservation of memory in PCAD and apparent memory decline in MCI. Our data suggest that marked changes occur at the protein level in MCI that may cause or reflect memory loss and other AD symptoms.

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Crossref · 10.3233/jad-2010-101083

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Authors
Aluise, C. D., Robinson, R. A. S., Cai, J., Pierce, W. M., Markesbery, W. R., & Butterfield, D. A.
Affiliations
  • University of Kentucky (US)
  • University of Louisville (US)
Venue
Journal of Alzheimer's Disease
Published
2011-02-26
Volume
23
Issue
2
Pages
257-269
ISSN
  • 1387-2877 (ISSN-L)
  • 1875-8908
OpenAlex
W1506952168
Cited by
94
References
60
Citations by year
2026: 1 · 2025: 1 · 2024: 4 · 2023: 3 · 2022: 6 · 2021: 8 · 2020: 6 · 2019: 7 · 2018: 5 · 2017: 5 · 2016: 7 · 2015: 8 · 2014: 8 · 2013: 7 · 2012: 12
FWCI
7.20
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of Alzheimer s Disease (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • University of Kentucky
  • National Institutes of Health
  • National Institute on Aging (P01AG005119, AG0 5119)
  • NIH Clinical Center
Topics
  • Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
  • Health Sciences › Medicine › Psychiatry and Mental health › Dementia and Cognitive Impairment Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
Keywords
amnestic mild cognitive impairment, preclinical Alzheimer's disease
MeSH terms
Aged, 80 and over, Alzheimer Disease, Amnesia, Analysis of Variance, Brain, Cognition Disorders, Female, Humans, Male, Memory, Oxidation-Reduction, Mass Spectrometry, Blotting, Western, Electrophoresis, Gel, Two-Dimensional, Neurofibrillary Tangles, Oxidative Stress, Statistics, Nonparametric, Proteomics, Plaque, Amyloid

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