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Cited by 304Iron Metabolism and DisordersImmune cells in cancerFerroptosis and cancer prognosis

Polarization dictates iron handling by inflammatory and alternatively activated macrophages

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Corna, G., Campana, L., Pignatti, E., Castiglioni, A., Tagliafico, E., Bosurgi, L., Campanella, A., Brunelli, S. +5 more·2010·Haematologica
DOI 10.3324/haematol.2010.023879PMC PMC2966902
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Abstract

BACKGROUND: Macrophages play a key role in iron homeostasis. In peripheral tissues, they are known to polarize into classically activated (or M1) macrophages and alternatively activated (or M2) macrophages. Little is known on whether the polarization program influences the ability of macrophages to store or recycle iron and the molecular machinery involved in the processes. DESIGN AND METHODS: Inflammatory/M1 and alternatively activated/M2 macrophages were propagated in vitro from mouse bone-marrow precursors and polarized in the presence of recombinant interferon-γ or interleukin-4. We characterized and compared their ability to handle radioactive iron, the characteristics of the intracellular iron pools and the expression of molecules involved in internalization, storage and export of the metal. Moreover we verified the influence of iron on the relative ability of polarized macrophages to activate antigen-specific T cells. RESULTS: M1 macrophages have low iron regulatory protein 1 and 2 binding activity, express high levels of ferritin H, low levels of transferrin receptor 1 and internalize--albeit with low efficiency -iron only when its extracellular concentration is high. In contrast, M2 macrophages have high iron regulatory protein binding activity, express low levels of ferritin H and high levels of transferrin receptor 1. M2 macrophages have a larger intracellular labile iron pool, effectively take up and spontaneously release iron at low concentrations and have limited storage ability. Iron export correlates with the expression of ferroportin, which is higher in M2 macrophages. M1 and M2 cells activate antigen-specific, MHC class II-restricted T cells. In the absence of the metal, only M1 macrophages are effective. CONCLUSIONS: Cytokines that drive macrophage polarization ultimately control iron handling, leading to the differentiation of macrophages into a subset which has a relatively sealed intracellular iron content (M1) or into a subset endowed with the ability to recycle the metal (M2).

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  • Macrophages associated with tumours might increase iron release in the tumour microenvironment.

    “tumour-associated macrophages may promote iron release in the tumour microenvironments40,41.”

    From Targeting cancer vulnerabilities with high-dose vitamin C (2019) · cites this paper as [40]

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Research works printed but not linked: 5

  1. Corna G, Santambrogio P, Minotti G, Cairo G. Doxorubicin paradoxically protects car- diomyocytes against iron-mediated toxici- ty: role of reactive oxygen species and fer- ritin. J Biol Chem. 2004;279(14):13738-45.
  2. Rovere P, Sabbadini MG, Vallinoto C, Fascio U, Rescigno M, Crosti MC, et al. Dendritic cell presentation of antigens from apoptotic cells in a pro-inflammatory con- text: role of opsonizing anti-beta 2 glyco- protein I antibodies. Arthritis Rheum. 1999;42(7):1412-20.
  3. Martinez FO, Sica A, Mantovani A, Locati M. Macrophage activation and polariza- tion. Front Biosci. 2008;13:453-61.
  4. Kakhlon O, Cabantchik ZI. The labile iron pool: characterization, measurement, and participation in cellular processes(1). Free Radic Biol Med. 2002;33(8):1037-46.
  5. Muckenthaler M. 6th International Conference on Heme Oxygenase-Heme Oxygenases in Biology and Medicine. September 30th -October 4th, 2009, Miami Beach, Florida. 2009.

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Authors
Corna, G., Campana, L., Pignatti, E., Castiglioni, A., Tagliafico, E., Bosurgi, L., Campanella, A., Brunelli, S., Manfredi, A. A., Apostoli, P., Silvestri, L., Camaschella, C., & Rovere-Querini, P.
Affiliations
  • Vita-Salute San Raffaele University (IT)
  • University of Modena and Reggio Emilia (IT)
  • IRCCS Ospedale San Raffaele (IT)
  • University of Brescia (IT)
ORCID
Venue
Haematologica
Publisher
Ferrata Storti Foundation (Haematologica)
Published
2010-05-29
Volume
95
Issue
11
Pages
1814-1822
ISSN
  • 0390-6078 (ISSN-L)
  • 1592-8721
DOI
10.3324/haematol.2010.023879
PMC
PMC2966902
OpenAlex
W2096008021
Cited by
304
References
47
Citations by year
2026: 21 · 2025: 12 · 2024: 25 · 2023: 17 · 2022: 32 · 2021: 23 · 2020: 13 · 2019: 20 · 2018: 22 · 2017: 23 · 2016: 18 · 2015: 14 · 2014: 17 · 2013: 22 · 2012: 15
FWCI
9.35
Type
journal article
Language
en
Open access
Locations
  • Haematologica (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • IRIS UNIMORE (University of Modena and Reggio Emilia) (submittedVersion, cc-by)
  • BOA (University of Milano-Bicocca) (submittedVersion, cc-by-nc)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
  • Institutional Research Information System (Università degli Studi di Brescia) (submittedVersion)
  • Archivio Istituzionale della Ricerca - Università Vita-Salute San Raffaele (submittedVersion)
Funders
  • Fondazione Cariplo
  • Ministero della Salute
Topics
  • Health Sciences › Medicine › Hematology › Iron Metabolism and Disorders
  • Life Sciences › Immunology and Microbiology › Immunology › Immune cells in cancer
  • Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Ferroptosis and cancer prognosis
Keywords
macrophage polarization, iron homeostasis, M1 macrophages, M2 macrophages, iron recycling, H-ferritin, transferrin receptor 1, ferroportin, iron regulatory proteins, labile iron pool, interferon-gamma, IL-4, T cell activation
MeSH terms
Ferroportin, Animals, Apoferritins, Gene Expression Regulation, Inflammation, Interferon-gamma, Iron, Lymphocyte Activation, Macrophage Activation, Macrophages, Receptors, Transferrin, T-Lymphocytes, Interleukin-4, Cation Transport Proteins, Iron Regulatory Protein 1, Iron Regulatory Protein 2, Mice

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