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Cited by 26Tissue Engineering and Regenerative Medicine3D Printing in Biomedical ResearchOrgan Transplantation Techniques and Outcomes

Re-Endothelialization of Decellularized Liver Scaffolds: A Step for Bioengineered Liver Transplantation

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Li, Kewei, Tharwat, Mohammad, Larson, Ellen L., Felgendreff, Philipp, Hosseiniasl, Seyed M., Rmilah, Anan Abu, Safwat, Khaled, Ross, Jeffrey J. +1 more·2022·Frontiers in Bioengineering and Biotechnology
DOI 10.3389/fbioe.2022.833163PMC PMC8960611
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Abstract

Bioengineered livers (BELs) are an attractive therapeutic alternative to address the donor organ shortage for liver transplantation. The goal of BELs technology aims at replacement or regeneration of the native human liver. A variety of approaches have been proposed for tissue engineering of transplantable livers; the current review will highlight the decellularization-recellularization approach to BELs. For example, vascular patency and appropriate cell distribution and expansion are critical components in the production of successful BELs. Proper solutions to these components of BELs have challenged its development. Several strategies, such as heparin immobilization, heparin-gelatin, REDV peptide, and anti-CD31 aptamer have been developed to extend the vascular patency of revascularized bioengineered livers (rBELs). Other novel methods have been developed to enhance cell seeding of parenchymal cells and to increase graft functionality during both bench and in vivo perfusion. These enhanced methods have been associated with up to 15 days of survival in large animal (porcine) models of heterotopic transplantation but have not yet permitted extended survival after implantation of BELs in the orthotopic position. This review will highlight both the remaining challenges and the potential for clinical application of functional bioengineered grafts.

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  • Reconstruction of the vascular network becomes necessary to allow the perfusion of the graft with host blood, ensuring the supply of nutrients and oxygen to different regions of the bioengineered organ.

    “Therefore, reconstruction of the vascular network becomes necessary to allow the perfusion of the graft with host blood, ensuring the supply of nutrients and oxygen to different regions of the bioengineered organ [14,222].”

    From Liver tissue engineering using decellularized scaffolds: Current progress, challenges, and opportunities (2024) · cites this paper as [222]

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Cited by

Sorted by citations

Authors
Li, K., Tharwat, M., Larson, E. L., Felgendreff, P., Hosseiniasl, S. M., Rmilah, A. A., Safwat, K., Ross, J. J., & Nyberg, S. L.
Affiliations
  • Mayo Clinic (US)
  • Zagazig University (EG)
  • Miromatrix Medical (United States) (US)
Corresponding author
Nyberg, Scott L.
ORCID
Venue
Frontiers in Bioengineering and Biotechnology
Publisher
Frontiers Media SA
Published
2022-03-10
Volume
10
Pages
833163-833163
ISSN
2296-4185 (ISSN-L)
DOI
10.3389/fbioe.2022.833163
PMC
PMC8960611
OpenAlex
W4220855280
Cited by
26
References
7
Citations by year
2026: 3 · 2025: 4 · 2024: 6 · 2023: 8 · 2022: 5
FWCI
1.37
Type
journal article
Language
en
Open access
Licence
Locations
  • Frontiers in Bioengineering and Biotechnology (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • Common Library Network (Der Gemeinsame Bibliotheksverbund) (submittedVersion, other-oa)
  • Greater South Information System
Funders
National Institute of Diabetes and Digestive and Kidney Diseases
Topics
  • Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
  • Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
  • Health Sciences › Medicine › Surgery › Organ Transplantation Techniques and Outcomes
Keywords
decellularization, re-endothelialization, recellularization, liver transplantation, vascular patency, heparin immobilization, REDV peptide, cell seeding, graft survival, heterotopic transplantation, orthotopic transplantation, porcine model

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