Probiotic potential and safety evaluation of Enterococcus faecalis OB14 and OB15, isolated from traditional tunisian testouri cheese and rigouta, using physiological and genomic analysis
Open accessAbstract
Lactic acid bacteria (LAB) strains OB14 and OB15 were isolated from traditional Tunisian fermented dairy products, Testouri cheese and Rigouta, respectively. They were identified as Enterococcus faecalis by the MALDI TOF-MS (matrix assisted laser desorption-ionization time of flight mass spectrometry) biotyper system and molecular assays (species-specific PCR). These new isolates were evaluated for probiotic properties, compared to E. faecalis Symbioflor 1 clone DSM 16431, as probiotic reference. The bacteria were found to be tolerant to the harsh conditions of the gastrointestinal tract (acidity and bile salt). They were low to moderate biofilm producers, can adhere to Caco-2/TC7 intestinal cells and strengthen the intestinal barrier through the increase of the transepithelial electrical resistance (TER). Susceptibility to ampicillin, vancomycin, gentamicin and erythromycin has been tested using the broth microdilutions method. The results demonstrated that E. faecalis OB14 and OB15 were sensitive to the clinically important ampicillin (MIC=1µg/mL) and vancomycin (MIC= 2µg/mL) antibiotics. However, Whole Genome Sequencing (WGS) showed the presence of tetracycline resistance and cytolysin genes in E. faecalis OB14, and this led to high mortality of Galleria Mellonella larvae in the virulence test. Hierarchical cluster analysis by MALDI TOF-MS biotyper showed that E. faecalis OB15 was closely related to the E. faecalis Symbioflor 1 probiotic strain than to OB14, and this has been confirmed by WGS using the Average Nucleotide Identity (ANI) and Genome-to-Genome Hybridization similarity (GGDH) methods. According to these results, E. faecalis OB15 seems to be reliable for future development as probiotic, in food or feed industry.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The criteria for selecting probiotic LAB strains are safety, including absence of secretion of harmful metabolites, survival in gastrointestinal conditions, and ability to inhibit pathogens.
“The criteria for selecting probiotic LAB strains are safety, including the absence of the secretion of harmful metabolites, survival in gastrointestinal conditions, and the ability to inhibit pathogens [10,11].”
From Probiotic and Antifungal Attributes of Lactic Acid Bacteria Isolates from Naturally Fermented Brazilian Table Olives (2022) · cites this paper as [10]
In Results and Discussion (page not available), the paper identifies survival under stomach and intestinal conditions as a prerequisite for probiotics; its safety evaluation also calls for assessing hemolysis and cytotoxicity. The Introduction describes inhibition of pathogens by bacteriocins as a proposed probiotic selection trait. The available passages do not identify absence of secretion of harmful metabolites as a selection criterion. (LLM, conf=0.84)
Experimental — accuracy not yet measured
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Trimmomatic: a flexible trimmer for Illumina sequence data
Bolger, A. M., Lohse, M., & Usadel, B. (2014) · Bioinformatics · doi:10.1093/bioinformatics/btu170 · Cited by 65,548 · cited 1× · see citing works
“Paired-end reads were trimmed using Trimmomatic v.0.36 (Bolger et al., 2014).”
MethodInfluentialSPAdes: A New Genome Assembly Algorithm and Its Applications to Single-Cell Sequencing
Bankevich, A., Nurk, S., Antipov, D., Gurevich, A. A., Dvorkin, M. V., Kulikov, A. S., Lesin, V. M., Nikolenko, S., Pham, S., Prjibelski, A. D., et al. (2012) · Journal of Computational Biology · doi:10.1089/cmb.2012.0021 · Cited by 25,691 · cited 2× · see citing works
“Sequence data quality was checked using FastQC v.0.11.6.1 Assembly of paired-end reads was done de novo using Spades software package v.3.12.0 (Bankevich et al., 2012) using its in-build read error correction functionality of BayesHammer (Nikolenko et al., 2013) by setting the “carefull” option,…”
MethodInfluentialGenome sequence-based species delimitation with confidence intervals and improved distance functions
Meier‐Kolthoff, J. P., Auch, A. F., Klenk, H., & Göker, M. (2013) · BMC Bioinformatics · doi:10.1186/1471-2105-14-60 · Cited by 6,761 · see citing works
Mauve: Multiple alignment of conserved genomic sequence with rearrangements
Darling, A. C. E., Mau, B., Blattner, F. R., & Perna, N. T. (2004) · Genome Research · doi:10.1101/gr.2289704 · Cited by 4,949 · see citing works
Adherence of bacteria to hydrocarbons: A simple method for measuring cell-surface hydrophobicity
Rosenberg, M., Gutnick, D. L., & ROSENBERG, E. B. (1980) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.1980.tb05599.x · Cited by 2,298 · see citing works
VFDB 2016: hierarchical and refined dataset for big data analysis—10 years on
Chen, L., Zheng, D., Liu, B., Yang, J., & Jin, Q. (2015) · Nucleic Acids Research · doi:10.1093/nar/gkv1239 · Cited by 1,672 · cited 2× · see citing works
“7 (Seemann, 2018), a mass screening software package of contigs, for resistome and virulome coding-genes detection, was used to screen against the NCBI Bacterial Antimicrobial Resistance Reference Gene Database (NCBI BARRGD, PRJNA313047), and Virulence Factors of Pathogenic Bacteria Database (VFDB; Chen et al., 2016),…”
MethodInfluentialGenomics and taxonomy in diagnostics for food security: soft-rotting enterobacterial plant pathogens
Pritchard, L., Glover, R. H., Humphris, S. N., Elphinstone, J. G., & Toth, I. K. (2015) · Analytical Methods · doi:10.1039/c5ay02550h · Cited by 1,597 · see citing works
Versatile genome assembly evaluation with QUAST-LG
Mikheenko, A., Prjibelski, A. D., Saveliev, V., Antipov, D., & Gurevich, A. A. (2018) · Bioinformatics · doi:10.1093/bioinformatics/bty266 · Cited by 1,537 · see citing works
The role and application of enterococci in food and health
Foulquié-Moreno, M. R., Sarantinopoulos, P., Tsakalidou, E., & Vuyst, L. D. (2005) · International Journal of Food Microbiology · doi:10.1016/j.ijfoodmicro.2005.06.026 · Cited by 1,075 · see citing works
Probiotics: an overview of beneficial effects
Ouwehand, A. C., Salminen, S., & Isolauri, E. (2002) · Antonie van Leeuwenhoek · doi:10.1023/a:1020620607611 · Cited by 1,045 · see citing works
Dunne, C., O’Mahony, L., Murphy, L., Thornton, G., Morrissey, D., O’Halloran, S., Feeney, M., Flynn, S., Fitzgerald, G., Daly, C., et al. (2001) · The American Journal of Clinical Nutrition · doi:10.1093/ajcn/73.2.386s · Cited by 922 · see citing works
(FEEDAP), E. P. O. A. A. P. O. S. U. I. A. F. (2012) · EFSA Journal · doi:10.2903/j.efsa.2012.2740 · Cited by 904 · see citing works
Enterococci as probiotics and their implications in food safety
Franz, C. M. A. P., Huch, M., Abriouel, H., Holzapfel, W. H., & Gálvez, A. (2011) · International Journal of Food Microbiology · doi:10.1016/j.ijfoodmicro.2011.08.014 · Cited by 795 · see citing works
Bernet‐Camard, M., Brassart, D., Neeser, J., & Servin, A. L. (1994) · Gut · doi:10.1136/gut.35.4.483 · Cited by 751 · see citing works
Adhesion, autoaggregation and hydrophobicity of 13 strains of Bifidobacterium longum
Del Re, B., Sgorbati, B., Miglioli, M., & Palenzona, D. (2000) · Letters in Applied Microbiology · doi:10.1046/j.1365-2672.2000.00845.x · Cited by 731 · cited 1× · see citing works
“Autoaggregation test was performed as described by Del Re et al. (2000) and Collado et al. (2008) including slight modifications.”
MethodAdhesion and aggregation properties of probiotic and pathogen strains
Collado, M. C., Meriluoto, J., & Salminen, S. (2008) · European Food Research and Technology · doi:10.1007/s00217-007-0632-x · Cited by 603 · see citing works
Enterococci in foods—a conundrum for food safety
Franz, C. M. A. P., Stiles, M. E., Schleifer, K. H., & Holzapfel, W. H. (2003) · International Journal of Food Microbiology · doi:10.1016/s0168-1605(03)00174-0 · Cited by 572 · see citing works
The Genus Enterococcus: Between Probiotic Potential and Safety Concerns—An Update
Hanchi, H., Mottawea, W., Sebei, K., & Hammami, R. (2018) · Frontiers in Microbiology · doi:10.3389/fmicb.2018.01791 · Cited by 569 · cited 2× · see citing works
“Indeed, although some strains of Enterococcus have been previously used for their technological properties, or as probiotic for humans and animals, a case-by-case-evaluation is needed (Araújo and Ferreira, 2013; Hanchi et al., 2018).”
BackgroundBayesHammer: Bayesian clustering for error correction in single-cell sequencing
Nikolenko, S., Korobeynikov, A., & Alekseyev, M. A. (2013) · BMC Genomics · doi:10.1186/1471-2164-14-s1-s7 · Cited by 479 · see citing works
(BIOHAZ), E. P. O. B. H., Ricci, A., Allende, A., Bolton, D., Chemaly, M., Davies, R., Gironés, R., Herman, L., Koutsoumanis, K. P., Lindqvist, R., et al. (2017) · EFSA Journal · doi:10.2903/j.efsa.2017.4664 · Cited by 460 · see citing works
Relationships between Enterococcal Virulence and Antimicrobial Resistance
Mundy, L. M., Sahm, D. F., & Gilmore, M. S. (2000) · Clinical Microbiology Reviews · doi:10.1128/cmr.13.4.513 · Cited by 440 · see citing works
Asadullah, K., Sterry, W., Stephanek, K., Jasulaitis, D., Leupold, M., Audring, H., Volk, H., & Döcke, W. D. (1998) · Journal of Clinical Investigation · doi:10.1172/jci1476 · Cited by 413 · cited 1× · see citing works
“IL-10 has interesting impact on immunoregulation, since it inhibits type 1/proinflammatory cytokine formation (Asadullah et al., 1998).”
BackgroundSafety assessment of dairy microorganisms: The Enterococcus genus
Ogier, J.-C., & Serror, P. (2007) · International Journal of Food Microbiology · doi:10.1016/j.ijfoodmicro.2007.08.017 · Cited by 404 · see citing works
Ramos, C. L., Thorsen, L., Schwan, R. F., & Jespersen, L. (2013) · Food Microbiology · doi:10.1016/j.fm.2013.03.010 · Cited by 360 · see citing works
Gómez, N. C., Ramiro, J. M. P., Quecán, B. X. V., & Franco, B. D. G. D. M. (2016) · Frontiers in Microbiology · doi:10.3389/fmicb.2016.00863 · Cited by 327 · see citing works
Selection and design of probiotics
Klaenhammer, T. R. (1999) · International Journal of Food Microbiology · doi:10.1016/s0168-1605(99)00076-8 · Cited by 317 · cited 1× · see citing works
“These findings are in accordance with previous studies that showed that Enterococcus species had the highest tolerance capability in the bile salts conditions among LAB group (Klaenhammer and Kullen, 1999).”
ResultHorizontal gene transfer and the genomics of enterococcal antibiotic resistance
Palmer, K. L., Kos, V. N., & Gilmore, M. S. (2010) · Current Opinion in Microbiology · doi:10.1016/j.mib.2010.08.004 · Cited by 307 · see citing works
Mannu, L., Paba, A., Daga, E., Comunian, R., Zanetti, S., Dupré, I., & Sechi, L. A. (2003) · International Journal of Food Microbiology · doi:10.1016/s0168-1605(03)00191-0 · Cited by 290 · see citing works
The Insect Galleria mellonella as a Powerful Infection Model to Investigate Bacterial Pathogenesis
Ramarao, N., Nielsen‐LeRoux, C., & Lereclus, D. (2012) · Journal of Visualized Experiments · doi:10.3791/4392 · Cited by 278 · see citing works
Pelletier, C., Bouley, C., Cayuela, C., Bouttier, S., Bourlioux, P., & Bellon‐Fontaine, M. (1997) · Applied and Environmental Microbiology · doi:10.1128/aem.63.5.1725-1731.1997 · Cited by 275 · see citing works
Enterococcal Infection—Treatment and Antibiotic Resistance
Kristich, C. J., Rice, L. B., & Arias, C. A. (2014) · Europe PMC (PubMed Central) · Cited by 274 · see citing works
Adhesion of human Lactobacillus acidophilus strain LB to human enterocyte-like Caco-2 cells
CHAUVIERE, G., COCONNIER, M.-H., KERNEIS, S., FOURNIAT, J., & SERVIN, A. L. (1992) · Journal of General Microbiology · doi:10.1099/00221287-138-8-1689 · Cited by 249 · cited 1× · see citing works
“Caco-2 cell line has been frequently used as an in vitro model to screen for adhesive strains (Elo et al., 1991; Chauvière et al., 1992; Bernet et al., 1994).”
MethodRelationships between Enterococcal Virulence and Antimicrobial Resistance
Mundy, L., Sahm, D. F., & Gilmore, M. S. (2000) · Clinical Microbiology Reviews · doi:10.1128/cmr.13.4.513-522.2000 · Cited by 236 · see citing works
Clinical Applications of Probiotic Bacteria
Salminen, S., Ouwehand, A. C., & Isolauri, E. (1998) · International Dairy Journal · doi:10.1016/s0958-6946(98)00077-6 · Cited by 229 · cited 2× · see citing works
“However, many of the resistance observed are intrinsic or natural resistance and non-transmissible (Salminen et al., 1998; Voravuthikunchai et al., 2006).”
BackgroundAssessing the Risk of Probiotic Dietary Supplements in the Context of Antibiotic Resistance
Zheng, M., Zhang, R., Tian, X., Zhou, X., Pan, X., & Wong, A. (2017) · Frontiers in Microbiology · doi:10.3389/fmicb.2017.00908 · Cited by 218 · see citing works
Sogawa, K., Watanabe, M., Sato, K., Segawa, S., Ishii, C., Miyabe, A., Murata, S., Saito, T., & Nomura, F. (2011) · Analytical and Bioanalytical Chemistry · doi:10.1007/s00216-011-4877-7 · Cited by 211 · cited 2× · see citing works
“0, which allows to identify at the species level with no doubt (Sogawa et al., 2011).”
BackgroundAnderson, A. C., Jonas, D., Huber, I., Karygianni, L., Wölber, J., Hellwig, E., Arweiler, N. B., Vach, K., Wittmer, A., & Al‐Ahmad, A. (2016) · Frontiers in Microbiology · doi:10.3389/fmicb.2015.01534 · Cited by 208 · see citing works
Enterocins in food preservation
Khan, H., Flint, S., & Yu, P. (2010) · International Journal of Food Microbiology · doi:10.1016/j.ijfoodmicro.2010.03.005 · Cited by 200 · cited 1× · see citing works
“Some strains have been proposed for food preservation, as they produce bacteriocins (enterocins), antimicrobial peptides with the potential to inhibit the growth of food-borne pathogenic and spoilage bacteria (Khan et al., 2010; Franz et al., 2011).”
BackgroundSemedo‐Lemsaddek, T., Santos, M. A., Martins, P., Lopes, M. F. S., Marques, J. J. F., Tenreiro, R. P., & Crespo, M. T. B. (2003) · Journal of Clinical Microbiology · doi:10.1128/jcm.41.6.2569-2576.2003 · Cited by 190 · cited 2× · see citing works
“Sheep red blood cells were less susceptible than rabbit, human and horse red blood cells (Semedo et al., 2003).”
BackgroundMethodGoldin, B. R., & Gorbach, S. L. (1992) · Springer eBooks · doi:10.1007/978-94-011-2364-8_13 · Cited by 184 · cited 1× · see citing works
“This concentration has been recommended as suitable for screening probiotic, as it simulates the conditions within the gastrointestinal tract (Goldin and Gorbach, 1992).”
BackgroundThe controversial role of Enterococcus faecalis in colorectal cancer
Almeida, C. V. D., Taddei, A., & Amedei, A. (2018) · Therapeutic Advances in Gastroenterology · doi:10.1177/1756284818783606 · Cited by 169 · cited 1× · see citing works
“Recently, a role of E. faecalis in pancreatic and colorectal cancers has also been suggested but this remains controversial (De Almeida et al., 2018; Maekawa et al., 2018).”
BackgroundElo, S., Saxelin, M., & Salminen, S. (1991) · Letters in Applied Microbiology · doi:10.1111/j.1472-765x.1991.tb00595.x · Cited by 151 · see citing works
Probiotic assessment of Enterococcus faecalis CP58 isolated from human gut
Nueno‐Palop, C., & Narbad, A. (2011) · International Journal of Food Microbiology · doi:10.1016/j.ijfoodmicro.2010.12.029 · Cited by 145 · see citing works
Are, A., Aronsson, L., Wang, S., Greicius, G., Lee, Y. K., Gustafsson, J.-Α., Pettersson, S., & Arulampalam, V. (2008) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0711734105 · Cited by 142 · see citing works
Zommiti, M., Cambronel, M., Maillot, O., Barreau, M., Sebei, K., Feuilloley, M. G. J., Ferchichi, M., & Connil, N. (2018) · Frontiers in Microbiology · doi:10.3389/fmicb.2018.01685 · Cited by 133 · see citing works
Diarra, M. S., Rempel, H., Champagne, J., Masson, L., Pritchard, J., & Topp, E. (2010) · Applied and Environmental Microbiology · doi:10.1128/aem.01545-10 · Cited by 126 · see citing works
Helwig, U. (2006) · World Journal of Gastroenterology · doi:10.3748/wjg.v12.i37.5978 · Cited by 124 · cited 1× · see citing works
“These differences among bacteria are not surprising since the immunomodulatory effects are often strain-specific (Helwig et al., 2006; Snel et al., 2011).”
BackgroundBenkerroum, N. (2013) · Comprehensive Reviews in Food Science and Food Safety · doi:10.1111/j.1541-4337.2012.00215.x · Cited by 123 · cited 1× · see citing works
“The composition of these products is often not well characterized, varying with regions and local markets, sometimes using raw material of poor microbiological quality, or lacking hygiene conditions (Benkerroum, 2013).”
BackgroundAntibiotic resistance: the pros and cons of probiotics
Courvalin, P. M. (2006) · Digestive and Liver Disease · doi:10.1016/s1590-8658(07)60006-1 · Cited by 122 · see citing works
Anandharaj, M., Sivasankari, B., Santhanakaruppu, R., Manimaran, M., Rani, R. P., & Sivakumar, S. (2015) · Research in Microbiology · doi:10.1016/j.resmic.2015.03.002 · Cited by 120 · cited 1× · see citing works
“The bile salts tolerance was tested as previously described by Anandharaj et al. (2015) with slight modifications.”
MethodDomann, E., Hain, T., Ghai, R., Billion, A., Kuenne, C. T., Zimmermann, K., & Chakraborty, T. (2007) · International Journal of Medical Microbiology · doi:10.1016/j.ijmm.2007.02.008 · Cited by 119 · see citing works
Cebrián, R., Baños, A., Valdivia, E., Pulido, R. P., Martínez‐Bueno, M., & Maqueda, M. (2011) · Food Microbiology · doi:10.1016/j.fm.2011.12.002 · Cited by 116 · cited 1× · see citing works
“These values have been considered has high capability to adhere to Caco-2 cells for Enterococcus strains with dairy origin (Cebrián et al., 2012; Pimentel et al., 2012).”
BackgroundProbiotic properties of lactic acid bacteria isolated from traditionally fermented Xinjiang cheese
Azat, R., Liu, Y., Li, W., Kayir, A., Lin, D.-B., Zhou, W.-W., & Zheng, X.-D. (2016) · Journal of Zhejiang University SCIENCE B · doi:10.1631/jzus.b1500250 · Cited by 116 · see citing works
Maekawa, T., Fukaya, R., Takamatsu, S., Itoyama, S., Fukuoka, T., Yamada, M., Hata, T., Nagaoka, S., Kawamoto, K., Eguchi, H., et al. (2018) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2018.10.169 · Cited by 106 · cited 1× · see citing works
“faecalis in pancreatic and colorectal cancers has also been suggested but this remains controversial (De Almeida et al., 2018; Maekawa et al., 2018).”
BackgroundAntagonistic activity against pathogenic bacteria by human vaginal lactobacilli
Voravuthikunchai, S. P., Bilasoi, S., & Supamala, O. (2006) · Anaerobe · doi:10.1016/j.anaerobe.2006.06.003 · Cited by 104 · cited 1× · see citing works
“However, many of the resistance observed are intrinsic or natural resistance and non-transmissible (Salminen et al., 1998; Voravuthikunchai et al., 2006).”
BackgroundMultilocus sequence typing by blast from de novo assemblies against PubMLST
Page, A. J., Taylor, B. E. W., & Keane, J. A. (2016) · The Journal of Open Source Software · doi:10.21105/joss.00118 · Cited by 103 · see citing works
Enterococcus faecalis from Healthy Infants Modulates Inflammation through MAPK Signaling Pathways
Wang, S., Hibberd, M. L., Pettersson, S., & Lee, Y. K. (2014) · PLoS ONE · doi:10.1371/journal.pone.0097523 · Cited by 98 · see citing works
García-González, N., Prete, R., Battista, N., & Corsetti, A. (2018) · Frontiers in Microbiology · doi:10.3389/fmicb.2018.02392 · Cited by 95 · cited 1× · see citing works
“Bacterial adhesion to epithelial cells is considered as one of the most reliable features for selection criteria of probiotic strains (Garcia-Gonzalez et al., 2018).”
BackgroundDNA Probe for Lactobacillus delbrueckii
Delley, M., Mollet, B., & Hottinger, H. (1990) · Applied and Environmental Microbiology · doi:10.1128/aem.56.6.1967-1970.1990 · Cited by 92 · see citing works
In vitro evaluation of the probiotic potential of Lactobacillus salivarius SMXD51
Messaoudi, S., Madi, A., Prévost, H., Feuilloley, M. G. J., Manaï, M., Dousset, X., & Connil, N. (2012) · Anaerobe · doi:10.1016/j.anaerobe.2012.10.004 · Cited by 90 · cited 1× · see citing works
“The cytotoxicity of the E. faecalis strains was tested on Caco-2/TC7 cells following overnight incubation with the bacteria, using the Neutral Red (NR) uptake test and an enzymatic assay (Cytotox 96 Promega, France), as described by Messaoudi et al. (2012) and Biaggini et al. (2017), respectively.”
MethodAntibiotic susceptibility of enterococci isolated from traditional fermented meat products
Barbosa, J. B., Ferreira, V. B., & Teixeira, P. C. M. (2009) · Food Microbiology · doi:10.1016/j.fm.2009.03.005 · Cited by 90 · cited 1× · see citing works
“Resistance to erythromycin seems to be intrinsic as well, non-transmissible, and is widely spread among enterococci commonly found in foods (Barbosa et al., 2009).”
BackgroundEnterococcal Bacteriocins and Antimicrobial Proteins that Contribute to Niche Control
Ness, I. F., Diep, D. B., & Ike, Y. (2014) · Europe PMC (PubMed Central) · Cited by 89 · see citing works
Popović, N., Dinić, M., Tolinački, M., Mihajlović, S., Terzić-Vidojević, A., Bojić, S., Đokić, J., Golić, N., & Veljović, K. (2018) · Frontiers in Microbiology · doi:10.3389/fmicb.2018.00078 · Cited by 86 · see citing works
Bujňáková, D., Strakova, E., & Kmeť, V. (2013) · Anaerobe · doi:10.1016/j.anaerobe.2013.10.009 · Cited by 81 · cited 1× · see citing works
“Adhesion to abiotic surfaces is not routinely investigated in probiotic strains, even if a positive correlation often exists between biofilm formation on abiotic surfaces and adhesiveness to biotic surfaces (Bujnakova et al., 2014).”
BackgroundProbiotic potential of Enterococcus faecalis strains isolated from meconium
Atya, A. K. A., Drider-Hadiouche, K., Ravallec, R., Silvain, A., Vachée, A., & Drider, D. (2015) · Frontiers in Microbiology · doi:10.3389/fmicb.2015.00227 · Cited by 78 · see citing works
Screening of Virulence Determinants in Enterococcus faecium Strains Isolated From Breast Milk
Reviriego, C., Eaton, T. J., Martı́n, R., Jiménez, E., Fernández, L., Gasson, M. J., & Rodríguez, J. M. (2005) · Journal of Human Lactation · doi:10.1177/0890334405275394 · Cited by 76 · see citing works
Li, B., Zhan, M., Evivie, S. E., Jin, D., Zhao, L., Chowdhury, S., Sarker, S. K., Huo, G., & Liu, F. (2018) · Frontiers in Microbiology · doi:10.3389/fmicb.2018.01943 · Cited by 74 · cited 1× · see citing works
“While the genus Enterococcus includes many species, only few have been studied to be used as probiotics, such as E. faecalis, E. faecium, E. lactis and more recently E. hirae (Adnan et al., 2017) and E. durans (Li et al., 2018).”
BackgroundAdnan, M., Patel, M. B., & Hadi, S. S. (2017) · PeerJ · doi:10.7717/peerj.3085 · Cited by 72 · cited 1× · see citing works
“While the genus Enterococcus includes many species, only few have been studied to be used as probiotics, such as E. faecalis, E. faecium, E. lactis and more recently E. hirae (Adnan et al., 2017) and E. durans (Li et al., 2018).”
BackgroundThe genus Enterococcus as probiotic: safety concerns
Araújo, T. F., & Ferreira, C. L. D. L. F. (2013) · Brazilian Archives of Biology and Technology · doi:10.1590/s1516-89132013000300014 · Cited by 70 · see citing works
Technologically important characteristics of Enterococcus isolates from milk and dairy products
Wessels, D., Jooste, P. J., & Mostert, J. F. (1990) · International Journal of Food Microbiology · doi:10.1016/0168-1605(90)90082-g · Cited by 67 · see citing works
Comparative study of normal and sensitive skin aerobic bacterial populations
Hillion, M., Mijouin, L., Jaouen, T., Barreau, M., Meunier, P., Lefeuvre, L., Lati, E., Chevalier, S., & Feuilloley, M. G. J. (2013) · MicrobiologyOpen · doi:10.1002/mbo3.138 · Cited by 65 · cited 1× · see citing works
“1 system, as previously described (Hillion et al., 2013; Zommiti et al., 2018).”
Biofilm-Forming Capacity in Biogenic Amine-Producing Bacteria Isolated from Dairy Products
Díaz, M., Ladero, V., del Río, B., Redruello, B., Fernández, M., Martín, M. C., & Álvarez, M. A. (2016) · Frontiers in Microbiology · doi:10.3389/fmicb.2016.00591 · Cited by 65 · see citing works
Haigh, J., Degun, A., Eydmann, M., Millar, M. R., & Wilks, M. (2011) · Journal of Clinical Microbiology · doi:10.1128/jcm.00576-11 · Cited by 63 · cited 1× · see citing works
“Formic acid was used on the bacterial spots as a quick extraction procedure (Haigh et al., 2011), then the MALDI target plate was introduced in the mass spectrometer for measurement and data acquisition.”
MethodExtremina, C. I., Costa, L., Aguiar, A. I., Peixe, L., & Fonseca, A. P. (2010) · Journal of Microbiological Methods · doi:10.1016/j.mimet.2010.11.007 · Cited by 62 · see citing works
Lactococcus sp. as Potential Probiotic Lactic Acid Bacteria
Kimoto‐Nira, H., Mizumachi, K., Nomura, M., Kobayashi, M., Fujita, Y., Okamoto, T., Suzuki, I., Tsuji, N. M., Kurisaki, J., & Ohmomo, S. (2007) · Japan Agricultural Research Quarterly JARQ · doi:10.6090/jarq.41.181 · Cited by 60 · cited 1× · see citing works
“It has been reported that the mechanism of adhesion to Caco-2/TC7 cells involves the combinations of carbohydrate and protein factors on the bacterial cell surface (Kimoto-Nira et al., 2007).”
BackgroundElbehiry, A., Marzouk, E., Hamada, M., Aldubaib, M., Alyamani, E. J., Moussa, I. M., AlRowaidhan, A., & Hemeg, H. A. (2017) · PubMed · Cited by 59 · see citing works
Snel, J., Vissers, Y. M., Smit, B., Jongen, J. M. J., van der Meulen, E. T., Zwijsen, R. M. L., Ruinemans‐Koerts, J., Jansen, A. P. H., Kleerebezem, M., & Savelkoul, H. F. J. (2010) · Clinical & Experimental Allergy · doi:10.1111/j.1365-2222.2010.03650.x · Cited by 52 · cited 1× · see citing works
“These differences among bacteria are not surprising since the immunomodulatory effects are often strain-specific (Helwig et al., 2006; Snel et al., 2011).”
BackgroundMultistep mechanism of probiotic bacterium, the effect on innate immune system
Amit‐Romach, E., Uni, Z., & Reifen, R. M. (2009) · Molecular Nutrition & Food Research · doi:10.1002/mnfr.200800591 · Cited by 43 · cited 1× · see citing works
“The ability of LAB to modulate cytokines production is considered as another criterion for the selection of beneficial microbes (Amit-Romach et al., 2010).”
BackgroundBraïek, O. B., Cremonesi, P., Morandi, S., Smaoui, S., Hani, K., & Ghrairi, T. (2018) · Microbial Pathogenesis · doi:10.1016/j.micpath.2018.03.005 · Cited by 42 · see citing works
Complete Genome Sequence of the Probiotic Enterococcus faecalis Symbioflor 1 Clone DSM 16431
Fritzenwanker, M., Kuenne, C. T., Billion, A., Hain, T., Zimmermann, K., Goesmann, A., Chakraborty, T., & Domann, E. (2013) · Genome Announcements · doi:10.1128/genomea.00165-12 · Cited by 41 · see citing works
Veljović, K., Popović, N., Miljković, M., Tolinački, M., Terzić-Vidojević, A., & Kojić, M. (2017) · Frontiers in Microbiology · doi:10.3389/fmicb.2017.01843 · Cited by 38 · see citing works
In vitrocomparison of commensal, probiotic and pathogenic strains ofEnterococcus faecalis
Christoffersen, T. E., Jensen, H., Kleiveland, C. R., Dørum, G., Jacobsen, M., & Lea, T. (2012) · British Journal Of Nutrition · doi:10.1017/s0007114512000220 · Cited by 34 · cited 1× · see citing works
“The complete genome sequence of this strain has been determined and several toxicological studies showed that the strain can be administred safely to humans (Domann et al., 2007; Christoffersen et al., 2012; Fritzenwanker et al., 2013).”
BackgroundLodemann, U., Strahlendorf, J., Schierack, P., Klingspor, S., Aschenbach, J. R., & Märtens, H. (2015) · Scientifica · doi:10.1155/2015/235184 · Cited by 32 · see citing works
Silvetti, T., Morandi, S., & Brasca, M. (2019) · Foodborne Pathogens and Disease · doi:10.1089/fpd.2018.2542 · Cited by 32 · cited 1× · see citing works
“faecalis isolated from raw milk cheeses does not represent a substantial reservoir of antimicrobial resistance and virulence factors if compared with clinical strains (Silvetti et al., 2019).”
BackgroundHaghshenas, B., Haghshenas, M., Nami, Y., Khosroushahi, A. Y., Abdullah, N., Barzegari, A., Rosli, R., & Hejazi, M. S. (2016) · PubMed Central · doi:10.15171/apb.2016.07 · Cited by 27 · cited 1× · see citing works
“3% oxgall with a survival rate of 98% after 3 h of incubation (Haghshenas et al., 2016), and E.”
BackgroundNeuhaus, K., Lamparter, M. C., Zölch, B., Landstorfer, R., Simon, S., Spanier, B., Ehrmann, M. A., & Vogel, R. F. (2016) · Archives of Microbiology · doi:10.1007/s00203-016-1291-8 · Cited by 23 · see citing works
CylA is a sequence-specific protease involved in toxin biosynthesis
Tang, W., Bobeica, S. C., Wang, L., & van der Donk, W. A. (2018) · Journal of Industrial Microbiology & Biotechnology · doi:10.1007/s10295-018-2110-9 · Cited by 23 · cited 1× · see citing works
“faecalis and acute patient mortality in the clinic (Tang et al., 2018).”
BackgroundProbiotic potential of enterococci isolated from canine feed
Lauková, A., Marciňáková, M., Strompfová, V., & Ouwehand, A. C. (2008) · Folia Microbiologica · doi:10.1007/s12223-008-0012-3 · Cited by 22 · cited 2× · see citing works
“Some strains of Enterococci, isolated from canine feed, were even found to be able to grow in the presence of 5% Oxgall (Lauková et al., 2008).”
BackgroundInfluentialBiaggini, K., Borrel, V., Szunerits, S., Boukherroub, R., N’Diaye, A. R., Zébré, A. C., Bonnin-Jusserand, M., Duflos, G., Feuilloley, M. G. J., Drider, D., et al. (2017) · Gut Pathogens · doi:10.1186/s13099-017-0171-3 · Cited by 19 · see citing works
Park, J., Jin, G.-D., Pak, J. I., Won, J., & Kim, E. B. (2017) · Current Microbiology · doi:10.1007/s00284-017-1210-5 · Cited by 17 · see citing works
Pimentel, L. L., Mättö, J., Malcata, F. X., Pintado, M., & Saarela, M. (2012) · International Dairy Journal · doi:10.1016/j.idairyj.2012.07.008 · Cited by 10 · see citing works
Al-Seraih, A. A., Belguesmia, Y., Cudennec, B., Baah, J., & Drider, D. (2017) · Probiotics and Antimicrobial Proteins · doi:10.1007/s12602-017-9357-8 · Cited by 7 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Probiotics Mechanism of Action on Immune Cells and Beneficial Effects on Human Health
Mazziotta, C., Tognon, M., Martini, F., Torreggiani, E., & Rotondo, J. C. (2023) · Cells · doi:10.3390/cells12010184 · Cited by 889 · see citing works
The Many Faces of Enterococcus spp.—Commensal, Probiotic and Opportunistic Pathogen
Krawczyk, B., Wityk, P., Gałęcka, M., & Michalik, M. (2021) · Microorganisms · doi:10.3390/microorganisms9091900 · Cited by 432 · see citing works
Engevik, M. A., Morra, C. N., Röth, D., Engevik, K. A., Spinler, J. K., Devaraj, S., Crawford, S. E., Estes, M. K., Kalkum, M., & Versalovic, J. A. (2019) · Frontiers in Microbiology · doi:10.3389/fmicb.2019.02305 · Cited by 206 · see citing works
“These microbes included three strains which are currently available as probiotics (L. reuteri ATCC PTA 6475, Enterococcus faecalis Symbioflor DSM 16431, and E. coli Nissle 1917) (Gronbach et al., 2010; Mu et al., 2018; Baccouri et al., 2019).”
BackgroundGrujović, M. Ž., Mladenović, K., Semedo‐Lemsaddek, T., Laranjo, M., Stefanović, O. D., & Kocić‐Tanackov, S. (2022) · Comprehensive Reviews in Food Science and Food Safety · doi:10.1111/1541-4337.12897 · Cited by 161 · see citing works
Current Trends of Enterococci in Dairy Products: A Comprehensive Review of Their Multiple Roles
Dapkevicius, M. D. L. E., Sgardioli, B. F., Câmara, S. P. D. A. E., Poeta, P. A. C. Q. D., & Malcata, F. X. (2021) · Foods · doi:10.3390/foods10040821 · Cited by 159 · see citing works
“faecalis strain from traditional Tunisian Rigouta increased by 28% the secretion of interleukin-10 in Caco-2/TC7 cells, an indication of its immunomodulatory potential [235].”
BackgroundRestoration of dysbiotic human gut microbiome for homeostasis
Dixit, K. K., Chaudhari, D. S., Dhotre, D. P., Shouche, Y. S., & Saroj, S. D. (2021) · Life Sciences · doi:10.1016/j.lfs.2021.119622 · Cited by 112 · see citing works
Mobile antimicrobial resistance genes in probiotics
Tóth, A. G., Csabai, I., Judge, M. F., Maróti, G., Becsei, Á., Spisák, S., & Solymosi, N. (2021) · doi:10.1101/2021.05.04.442546 · Cited by 97 · see citing works
Terzić-Vidojević, A., Veljović, K., Popović, N., Tolinački, M., & Golić, N. (2021) · Foods · doi:10.3390/foods10112753 · Cited by 75 · see citing works
“faecalis DSM 16431) has a history of long-term safe use, and its safety was proven by whole-genome sequencing (WGS) [142].”
BackgroundInfluentialLorente-Picón, M., & Laguna, A. (2021) · Biomolecules · doi:10.3390/biom11030433 · Cited by 69 · see citing works
Cambronel, M., Nilly, F., Mesguida, O., Boukerb, A. M., Racine, P., Baccouri, O., Borrel, V., Martel, J., Fécamp, F., Knowlton, R., et al. (2020) · Frontiers in Microbiology · doi:10.3389/fmicb.2020.01501 · Cited by 64 · see citing works
“In the pathogen E. coli O157:H7, the two-component systems QseBC and QseEF have been shown to be involved in the response to Epi and NE (Zhu et al., 2006; Rasko et al., 2008).”
BackgroundGao, Y., Liu, Y., Sun, M., Zhang, H., Mu, G., & Tuo, Y. (2020) · Journal of Dairy Science · doi:10.3168/jds.2019-18047 · Cited by 51 · see citing works
Enterococcus spp.: Is It a Bad Choice for a Good Use—A Conundrum to Solve?
Ferchichi, M., Sebei, K., Boukerb, A. M., Karray‐Bouraoui, N., Chevalier, S., Feuilloley, M. G. J., Connil, N., & Zommiti, M. (2021) · Microorganisms · doi:10.3390/microorganisms9112222 · Cited by 54 · see citing works
“Since the probiotic effect is strain dependent, it should be drastically characterized (phenotypically and genotypically) and must be safe and free of any pathogenicity such as the absence of virulence determinants and acquired antibiotic resistance genes [1,51].”
BackgroundProbiotic properties of bacteria isolated from bee bread of stingless bee Heterotrigona itama
Mohammad, S. M., Nor‐Khaizura, M. A. R., & Zawawi, N. (2020) · Journal of Apicultural Research · doi:10.1080/00218839.2020.1801152 · Cited by 52 · see citing works
Panthee, S., Paudel, A., Hamamoto, H., Ogasawara, A. A., Iwasa, T., Blom, J., & Sekimizu, K. (2021) · Genomics · doi:10.1016/j.ygeno.2021.03.021 · Cited by 51 · see citing works
Özkan, E. R., Demirci, T., & Akın, N. (2021) · LWT · doi:10.1016/j.lwt.2021.110908 · Cited by 50 · see citing works
Cambronel, M., Tortuel, D., Biaggini, K., Maillot, O., Taupin, L., Vallée‐Rehel, K., Rincé, I., Muller, C., Hardouin, J., Feuilloley, M. G. J., et al. (2019) · Scientific Reports · doi:10.1038/s41598-019-56666-7 · Cited by 46 · see citing works
Hussein, W. E., Abdelhamid, A. G., Rocha-Mendoza, D., García‐Cano, I., & Yousef, A. E. (2020) · Frontiers in Microbiology · doi:10.3389/fmicb.2020.608314 · Cited by 45 · see citing works
“…probiotic preparations are E. faecium NCIMB 10415(SF68), used in Cernivet R© (CerbiosPharma SA, Switzerland) and Cylactin R© (Hoffmann-La Roche, Basel, Switzerland), and E. faecalis Symbioflor R© 1 used in Symbioflor 1 (Symbiopharm, Herborn, Germany) (Serio et al., 2010; Baccouri et al., 2019).”
BackgroundMethodQuintieri, L., Fanelli, F., Monaci, L., & Fusco, V. (2024) · Foods · doi:10.3390/foods13040601 · Cited by 43 · see citing works
Probiotics: insights and new opportunities for Clostridioides difficile intervention
Pal, R., Athamneh, A. I. M., Deshpande, R., Ramirez, J. A. R., Adu, K. T., Muthuirulan, P., Pawar, S., Biazzo, M., Apidianakis, Y., Sundekilde, U. K., et al. (2022) · Critical Reviews in Microbiology · doi:10.1080/1040841x.2022.2072705 · Cited by 42 · see citing works
Kim, Y., Choi, S., Jeong, Y., & Kang, C.-H. (2022) · Microorganisms · doi:10.3390/microorganisms10102070 · Cited by 40 · see citing works
Exopolysaccharides produced by Enterococcus genus — An overview
Kavitake, D., Devi, P. B., Delattre, C., Reddy, G. B., & Shetty, P. H. (2022) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2022.12.042 · Cited by 40 · see citing works
Cruz‐Casas, D. E., Aguilar, C. N., Ascacio-Valdés, J. A., Rodrı́guez-Herrera, R., Chávez‐González, M. L., & Flores‐Gallegos, A. C. (2023) · Heliyon · doi:10.1016/j.heliyon.2023.e13491 · Cited by 36 · see citing works
In Vivo Functional Properties of Dairy Bacteria
Aprea, G., Matto, I. D., Tucci, P., Marino, L., Scattolini, S., & Rossi, F. (2023) · Microorganisms · doi:10.3390/microorganisms11071787 · Cited by 34 · see citing works
Enterococcus faecalis: implications for host health
Boeder, A. M., Spiller, F., Carlström, M., & Izídio, G. S. (2024) · World Journal of Microbiology and Biotechnology · doi:10.1007/s11274-024-04007-w · Cited by 35 · see citing works
“Furthermore, the absence of transferable antibiotic resistance genes and potential virulence determinants should be investigated in each new potential probiotic strain intended for use in the food or pharmaceutical industry (Baccouri et al. 2019).”
Diez-Gutiérrez, L., Vicente, L. S., Sáenz, J., Barrón, L. J. R., & Chávarri, M. (2022) · Journal of Functional Foods · doi:10.1016/j.jff.2022.105230 · Cited by 32 · see citing works
Daliri, E. B., Kim, Y., Do, Y., Chelliah, R., & Oh, D. (2021) · Probiotics and Antimicrobial Proteins · doi:10.1007/s12602-021-09798-0 · Cited by 32 · see citing works
“Some studies have suggested that the presence of certain cholesterol-binding functional groups on the cell wall peptidoglycan of probiotic bacteria could account for the ability of dead bacteria to reduce cholesterol levels [26].”
BackgroundSafety Evaluation of Lactobacillus delbrueckii subsp. lactis CIDCA 133: a Health-Promoting Bacteria
de Jesus, L. C. L., Sousa, T. J., Coelho-Rocha, N. D., Profeta, R., Barroso, F. A. L., Drumond, M. M., Mancha-Agresti, P. D. C., Ferreira, E. F., Brenig, B., Aburjaile, F. F., et al. (2021) · Probiotics and Antimicrobial Proteins · doi:10.1007/s12602-021-09826-z · Cited by 33 · see citing works
“Newly candidates probiotic strains after correct typing must be studied for their specific health-promoting effects and safety for human and veterinary consumption [39, 53] through both phenotypic analysis (in vitro tests and in vivo toxicity studies) and genomic approach [21, 25, 54].”
BackgroundTran, K.-D., Le-Thi, L., Hiếu, V. H., Dinh-Thi, T.-V., Nguyen-Thi, T., Phan, N.-H., & Nguyen, K.-U. (2023) · Probiotics and Antimicrobial Proteins · doi:10.1007/s12602-023-10114-1 · Cited by 28 · see citing works
“In addition, their probiotic properties that affect human health have also been demonstrated [2, 5–13].”
Hanifeh, M., Spillmann, T., Huhtinen, M. K., Sclivagnotis, Y. S., Grönthal, T. S. C., & Hynönen, U. (2021) · Animals · doi:10.3390/ani11113283 · Cited by 26 · see citing works
Simões, L., Fernandes, N., de Souza, A., dos Santos, L., Magnani, M., Abrunhosa, L., Teixeira, J., Schwan, R. F., & Dias, D. R. (2022) · Fermentation · doi:10.3390/fermentation8060277 · Cited by 23 · see citing works
Mefleh, M., Darwish, A. M. G., Mudgil, P., Maqsood, S., & Boukid, F. (2022) · Fermentation · doi:10.3390/fermentation8120743 · Cited by 24 · see citing works
Zinno, P., Perozzi, G., & Devirgiliis, C. (2023) · Microorganisms · doi:10.3390/microorganisms11071696 · Cited by 23 · see citing works
Zaghloul, H. A. H., & Halfawy, N. M. E. (2022) · Microbial Genomics · doi:10.1099/mgen.0.000896 · Cited by 23 · see citing works
“These characters are necessary for adhesion to intestinal cells and to form a barrier that prevents the colonization by pathogens [60].”
BackgroundUnban, K., Klongklaew, A., Kodchasee, P., Pamueangmun, P., Shetty, K., & Khanongnuch, C. (2022) · Insects · doi:10.3390/insects13020136 · Cited by 22 · see citing works
Molecular Assessment and Validation of the Selected Enterococcal Strains as Probiotics
Hussain, A., Akram, S., Ahmad, D., Rehman, M., Ahmed, A., & Ali, S. A. (2023) · Probiotics and Antimicrobial Proteins · doi:10.1007/s12602-023-10163-6 · Cited by 21 · see citing works
“According to Baccouri et al. [49], hydrophobicity and auto-aggregation help in adhesion to intestinal cell and prevent pathogen colonization; thus, these are considered beneficial properties of probiotics.”
InfluentialJaved, G. A., Arshad, N., Munir, A., Khan, S. Y., Rasheed, S., & Hussain, I. (2021) · International Dairy Journal · doi:10.1016/j.idairyj.2021.105297 · Cited by 21 · see citing works
Fusco, V., Fanelli, F., & Chieffi, D. (2023) · Foods · doi:10.3390/foods12203782 · Cited by 23 · see citing works
Ballén, V., Ratia, C., Cepas, V., & Soto, S. M. (2020) · Biofouling · doi:10.1080/08927014.2020.1824272 · Cited by 19 · see citing works
“E. faecalis has been proposed as well as a probiotic due to its adherence to 511 intestinal cells and reinforcement of the epithelial barrier (Baccouri et al. 2019).”
BackgroundFarhat, L. B., Romeo, F. V., Foti, P., Russo, N., Randazzo, C. L., Caggia, C., & Abidi, F. (2022) · Microorganisms · doi:10.3390/microorganisms10101876 · Cited by 19 · see citing works
“Acknowledgments: The present work was partially supported by the Tunisian Ministry of Higher Education and Scientific Research.”
MethodInfluentialMendez-Romero, J. I., Reyes‐Díaz, R., Santiago‐López, L., Hernández‐Mendoza, A., Vallejo‐Córdoba, B., Sáyago‐Ayerdi, S. G., Gómez‐Gil, B., & González-Córdova, A. F. (2021) · International Journal of Dairy Technology · doi:10.1111/1471-0307.12751 · Cited by 19 · see citing works
Li, O., Zhang, H., Wang, W., Liang, Y., Chen, W., Din, A. U., Li, L., & Zhou, Y. (2021) · Journal of Medical Microbiology · doi:10.1099/jmm.0.001397 · Cited by 19 · see citing works
Khushi, S. S., Sumon, M. S., Ahmmed, M. K., Zilani, M. N. H., Ahmmed, F., Giteru, S. G., & Sarower, M. G. (2020) · Aquaculture and Fisheries · doi:10.1016/j.aaf.2020.10.004 · Cited by 18 · see citing works
Fermentation Dynamics of Naturally Fermented Palm Beverages of West Bengal and Jharkhand in India
Das, S., & Tamang, J. P. (2023) · Fermentation · doi:10.3390/fermentation9030301 · Cited by 17 · see citing works
Yang, Z., Amal, F. E., Yang, L., Liu, Y., Zhu, L., Zhu, Z., & Jiang, L. (2022) · Frontiers in Microbiology · doi:10.3389/fmicb.2022.926710 · Cited by 16 · see citing works
“Antibiotic Resistance Testing Antibiotic resistance testing was performed using the minimum inhibitory concentration (MIC) method described in a previous study (Baccouri et al., 2019).”
MethodScardaci, R., Bietto, F., Racine, P., Boukerb, A. M., Lesouhaitier, O., Feuilloley, M. G. J., Scutera, S., Musso, T., Connil, N., & Pessione, E. (2022) · Microorganisms · doi:10.3390/microorganisms10030487 · Cited by 17 · see citing works
Scardaci, R., Varese, F., Manfredi, M., Marengo, E., Mazzoli, R., & Pessione, E. (2020) · Journal of Proteomics · doi:10.1016/j.jprot.2020.104003 · Cited by 17 · see citing works
Hwanhlem, N., Salaipeth, L., Charoensook, R., Kanjan, P., & Maneerat, S. (2022) · Journal of Microbiology and Biotechnology · doi:10.4014/jmb.2110.10040 · Cited by 14 · see citing works
“They are also frequently associated with nosocomial infections and have been implicated in human diseases such as bacteremia, endocarditis, and urinary tract infections [38].”
BackgroundKANAK, E. K., YILMAZ, S. Ö., Altındiş, M., Tanrıverdi, E. S., & Otlu, B. (2022) · Journal of Applied Microbiology · doi:10.1093/jambio/lxac005 · Cited by 14 · see citing works
Banić, M., Butorac, K., Čuljak, N., Butorac, A., Novak, J., Pavunc, A. L., Rušanac, A., Stanečić, Ž., Lovrić, M., Šušković, J., et al. (2024) · International Journal of Molecular Sciences · doi:10.3390/ijms25042431 · Cited by 15 · see citing works
Moghimani, M., Onyeaka, H. N., Hashemi, M., & Afshari, A. (2024) · Frontiers in Microbiology · doi:10.3389/fmicb.2024.1385301 · Cited by 13 · see citing works
Cruz‐Casas, D. E., Ramos‐González, R., Prado‐Barragán, L. A., Ilyina, A. D., Aguilar, C. N., Rodrı́guez-Herrera, R., Tsopmo, A., & Flores‐Gallegos, A. C. (2024) · Food Chemistry · doi:10.1016/j.foodchem.2024.141598 · Cited by 13 · see citing works
Lin, Y.-C., Wu, C.-Y., Huang, H., Lu, M., Hu, W., & Lee, K. (2021) · Frontiers in Microbiology · doi:10.3389/fmicb.2021.785351 · Cited by 14 · see citing works
“An autoaggregation assay was performed as described by Baccouri et al. and Kaur et al. with some modifications (Kaur et al., 2018; Baccouri et al., 2019).”
MethodEveno, M., Savard, P., Belguesmia, Y., Bazinet, L., Gancel, F., Drider, D., & Fliss, I. (2020) · Probiotics and Antimicrobial Proteins · doi:10.1007/s12602-020-09687-y · Cited by 13 · see citing works
“The key criteria for selecting a microbial strain as a probiotic are safety, survival under gastrointestinal conditions, and ability to inhibit pathogens [6].”
BackgroundMkadem, W., Belguith, K., Oussaief, O., El‐Hatmi, H., Indio, V., Savini, F., Cesare, A. D., & Boudhrioua, N. M. (2022) · Food Bioscience · doi:10.1016/j.fbio.2022.102275 · Cited by 11 · see citing works
Probiotic potential and safety assessment of bacteriocinogenic Enterococcus faecalis CAUM157
Elnar, A. G., & Kim, G. (2025) · Frontiers in Microbiology · doi:10.3389/fmicb.2025.1563444 · Cited by 11 · see citing works
Melia, S., Juliyarsi, I., Kurnia, Y. F., Aritonang, S. N., Purwati, E., Sukma, A., Fitria, N., Susmiati, S., Meinapuri, M., Pratama, Y. E., et al. (2023) · Journal of Advanced Veterinary and Animal Research · doi:10.5455/javar.2023.j653 · Cited by 11 · see citing works
“[30] explained that the peptidoglycan cell wall of probiotic bacteria binds the cholesterol even to dead bacteria to decrease the levels.”
BackgroundScardaci, R., Manfredi, M., Barberis, E., Scutera, S., Marengo, E., & Pessione, E. (2021) · Microbiology Research · doi:10.3390/microbiolres12030043 · Cited by 10 · see citing works
Farhat, L. B., Aissaoui, N., Torrijos, R., Luz, C., Meca, G., & Abidi, F. (2022) · Journal of Applied Microbiology · doi:10.1111/jam.15763 · Cited by 10 · see citing works
“9% NaCl (w/v)] and 100 μl of each dilution was inoculated on Man Rogosa Sharpe (MRS) agar and M17 agar and all plates were incubated for 48 h at 30°C under anaerobic conditions (Baccouri et al., 2019).”
MethodInfluentialGonzález, R. C., Henríquez‐Castillo, C., Lohrmann, K. B., Romero, M. S., Ramajo, L., Schmitt, P., & Brokordt, K. (2022) · Microorganisms · doi:10.3390/microorganisms10122330 · Cited by 9 · see citing works
Wu, C., Lin, X., Lin, T., Dai, C., Lv, H., Zhou, X., & Zhang, J. (2021) · Open Chemistry · doi:10.1515/chem-2021-0054 · Cited by 9 · see citing works
Loghmani, S. B., Zitzow, E., Koh, C. C., Ulmer, A., Veith, N., Großeholz, R., Rossnagel, M., Loesch, M., Aebersold, R. H., Kreikemeyer, B., et al. (2022) · Microbiology Spectrum · doi:10.1128/spectrum.02400-21 · Cited by 9 · see citing works
Probiotics in milk and dairy foods
da Motta, A. D. S., Nespolo, C. R., & Breyer, G. M. (2022) · Elsevier eBooks · doi:10.1016/b978-0-323-85170-1.00004-x · Cited by 8 · see citing works
Rahmani, M., Saffari, F., Domann, E., Zimmermann, K., Langroudi, L., & Mansouri, S. (2022) · Probiotics and Antimicrobial Proteins · doi:10.1007/s12602-022-09951-3 · Cited by 8 · see citing works
“faecalis Symbioflor 1 was used as positive control [30].”
BackgroundMethodInfluentialRobles‐Rodríguez, C., Cardoso-Carmona, D., González‐Dávalos, L., Lozano‐Flores, C., Páez-Trejo, A., Shimada, A. S., & Mora, O. (2023) · Ruminants · doi:10.3390/ruminants3040028 · Cited by 7 · see citing works
“Baccouri et al. [35], working with Enterococcus faecalis OB14, reported resistance to gentamicin and erythromycin.”
BackgroundLing, C., Yan, F., Lin, K., Ye, H., Cao, Q., Dong, Z., Zhang, C., Zuo, J., & Wang, W. (2025) · Poultry Science · doi:10.1016/j.psj.2025.105725 · Cited by 7 · see citing works
“…and acetic acids) to lower microenvironmental pH value, secrete antibacterial substances (e. g. bacteriocin) to destruct pathogenic bacterial cell membrane (Ladjouzi et al., 2025; Yin et al., 2024), as well as competitively inhibit pathogenic colonization of host intestine (Baccouri et al., 2019).”
Kim, H. J., Kwon, M. Y., Song, S., Cheon, S. W., & Kim, H. J. (2025) · World Journal of Microbiology and Biotechnology · doi:10.1007/s11274-025-04598-y · Cited by 8 · see citing works
“Streptococcus mutans , ATCC 6538 , and Enterococcus faecalis KCTC 3206 were used as controls for the hemolysis test, as described in previous studies (Baccouri et al. 2019; Wolff and Liljemark 1978 ; Xu et al. 2024).”
Probiotic Characterization of Lactic Acid Bacteria from Donkey Feces in China
Wu, Y., Yue, S., Yu, J., Bian, F., Gao, C., & Zhang, Y. (2025) · Animals · doi:10.3390/ani15020207 · Cited by 6 · see citing works
You, R., Kwon, O. Y., Woo, H. J., & Lee, S. H. (2021) · Food Science of Animal Resources · doi:10.5851/kosfa.2021.e65 · Cited by 6 · see citing works
Torres-Maravilla, E., Boucard, A.-S., Azzaz, J. A., Gontier, S., Kulakauskas, S., Langella, P., & Bermúdez‐Humarán, L. G. (2023) · Beneficial Microbes · doi:10.1163/18762891-20230004 · Cited by 6 · see citing works
Bioprotective potential of lactic acid bacteria
Laslo, É., György, É., & András, C. D. (2020) · Acta Universitatis Sapientiae Alimentaria · doi:10.2478/ausal-2020-0007 · Cited by 6 · see citing works
Probiotic potential of three Enterococcus spp. isolated from raw milk: An in vitro assessment
Acar, B. Ç., Yüksekdağ, Z., & Yılmaz, E. Ş. (2025) · International Dairy Journal · doi:10.1016/j.idairyj.2025.106196 · Cited by 5 · see citing works
Özdemir, N. (2023) · Applied Biochemistry and Biotechnology · doi:10.1007/s12010-023-04393-1 · Cited by 5 · see citing works
“Some strains of this genus, especially some of the Enterococcus faecalis strains, do not show pathogenicity [11].”
BackgroundChirakkara, S. P., & Abraham, A. (2022) · Biomedicine · doi:10.51248/.v42i6.2302 · Cited by 5 · see citing works
Baccouri, O., Boukerb, A. M., Nilly, F., Cambronel, M., Smaali, I., Feuilloley, M. G. J., Abidi, F., & Connil, N. (2020) · Microbiology Resource Announcements · doi:10.1128/mra.01433-19 · Cited by 5 · see citing works
“faecalis OB15 from rigouta, a popular traditional Tunisian fermented cheese that is often prepared by boiling acidified cheese whey from cow’s milk (7).”
BackgroundBadr, L., Yasir, M., Alkhaldy, A. A., Soliman, S. A., Ganash, M., Turkistani, S. A., Jiman-Fatani, A. A., Alzahrani, I., & Azhar, E. I. (2024) · PeerJ · doi:10.7717/peerj.18392 · Cited by 4 · see citing works
“Moreover, capsule formation and adhesion–related genes have also been reported in probiotic/starter strains of enterococci ( Baccouri et al., 2019 ).”
BackgroundAfolabi, I. S., Ahuekwe, E. F., Garuba, P. A., Adigun, A. J., Odutayo, O. E., & Adeyemi, A. O. (2022) · Fermentation · doi:10.3390/fermentation9010033 · Cited by 4 · see citing works
Afolabi, I. S., Adigun, A. J., Garuba, P. A., Ahuekwe, E. F., Odutayo, O. E., & Adeyemi, A. O. (2023) · Fermentation · doi:10.3390/fermentation9080707 · Cited by 4 · see citing works
Understanding the ambiguity behind Enterococcus as a probiotic ally or a pathogenic adversary
Elnar, A. G., & Kim, G. (2025) · Critical Reviews in Microbiology · doi:10.1080/1040841x.2025.2562926 · Cited by 3 · see citing works
“Nevertheless, members of this genus continue to be a key component of various traditional fermented food products, enhancing the sensory properties of food and providing beneficial effects to the consuming host (Baccouri et al. 2019; Dapkevicius et al. 2021; de Melo Pereira et al. 2022).”
Avgousti, K., Dushku, E., Spyropoulou, A., Kotzamanidis, C., Staikou, A. E., & Yiangou, M. (2025) · Developmental & Comparative Immunology · doi:10.1016/j.dci.2025.105481 · Cited by 3 · see citing works
Li, S., Deng, S., Zhang, P., Lu, Q., Pu, W., Ma, M., Li, S., Zhang, W., & Chen, C. (2025) · Fermentation · doi:10.3390/fermentation11100598 · Cited by 3 · see citing works
“These findings are consistent with previous reports [39,40].”
Hu, Q. C., Wu, H., Yu, M., Song, L., Feng, J., Ma, Y., Ding, F., Guo, L., & Wang, Z. (2025) · Frontiers in Agronomy · doi:10.3389/fagro.2025.1628083 · Cited by 3 · see citing works
Essa, M. O. A., Basher, N. S., Abdelhadi, L. A. M., Ibrahim, N. A., Rehman, S. U., Husien, H. M., Saleh, A. A., & Cheng, D. (2026) · Veterinary Sciences · doi:10.3390/vetsci13020120 · Cited by 4 · see citing works
“Additionally, they can be found in diverse environments such as agro-industrial waste, food, animal feed, soil, plants, and water [41].”
Billateh, A., Cheirsilp, B., & Suyotha, W. (2026) · Applied Food Research · doi:10.1016/j.afres.2026.102087 · Cited by 3 · see citing works
Traditional Mediterranean cheeses: Lactic acid bacteria populations and functional traits
Laranjo, M., & Potes, M. E. (2022) · Elsevier eBooks · doi:10.1016/b978-0-323-89875-1.00011-0 · Cited by 3 · see citing works
Nath, S., Paul, M., Paul, P., Chanda, P., Naha, A., Yumnam, H., & Sharma, I. (2025) · International Dairy Journal · doi:10.1016/j.idairyj.2025.106538 · Cited by 2 · see citing works
Selection Criteria for Identifying Putative Probiont
TEZEL, B. U., Şanlıbaba, P., Akçelik, N., & Akçelik, M. (2021) · Elsevier eBooks · doi:10.1016/b978-0-12-822909-5.00002-2 · Cited by 2 · see citing works
Kıymacı, M. E., Şimşek, D., Tok, K. C., Dirican, D., & Gumustas, M. (2023) · Journal of the Hellenic Veterinary Medical Society · doi:10.12681/jhvms.26743 · Cited by 2 · see citing works
Dharsono, H. D. A., Mujahid, M., Apriyanti, E., Adang, R. A. F., Putri, S. A., Satari, M. H., & Kurnia, D. (2023) · Research Journal of Pharmacy and Technology · doi:10.52711/0974-360x.2023.00744 · Cited by 2 · see citing works
Eminoğlu, G., Akal, C., & Özer, B. (2023) · Wiley eBooks · doi:10.1002/9781119776406.ch3 · Cited by 2 · see citing works
Characterization of novel potential probiotic strains of lactic acid bacteria from rat faeces
Dieudonné, N., Mbugua, A., & Kevin, M. (2024) · African Journal of Microbiology Research · doi:10.5897/ajmr2024.9742 · Cited by 1 · see citing works
“These findings align with previous studies where Enterococcus strains (Baccouri et al., 2019) and L. garvieae strains (Patel et al., 2020) were viable in acidic pH and high bile salt concentrations.”
BackgroundResultLü, X., Sang, W., & Pan, L. (2025) · Journal of Functional Foods · doi:10.1016/j.jff.2025.106659 · Cited by 1 · see citing works
Aziz, G., & Zaidi, A. (2025) · Microbiology · doi:10.1099/mic.0.001616 · Cited by 1 · see citing works
“Enterococcus species sourced from fermented foods generally lack gelatinase [72], as is the case here.”
Paul, S., Banerjee, S., Banerjee, S., & Goel, G. (2026) · Probiotics and Antimicrobial Proteins · doi:10.1007/s12602-026-10941-y · Cited by 1 · see citing works
“The same was determined using free radical scav - enging activity assays [ 59 , 60].”
Abed, S. M., Essa, R. H., & Yaseen, K. H. (2022) · AIP conference proceedings · doi:10.1063/5.0093648 · Cited by 1 · see citing works
Sana, M., Zitoun, O. A., Zidoune, M. N., Barreau, M., Boukerb, A. M., & Connil, N. (2025) · International Dairy Journal · doi:10.1016/j.idairyj.2025.106206 · Cited by 2 · see citing works
Indicator Microflora of Ducks and Chickens in Home Farm Conditions
Виговська, Л. М., Ushkalov, А. V., DAVYDOVSKA, L., Melnyk, V. V., Ushkalov, В., & Shevchenko, O. (2025) · Veterinary Sciences and Practices · doi:10.17094/vetsci.1577819 · Cited by 1 · see citing works
Chandran, A., Beena, A. K., Bhagya, S., Rathish, R. L., & Rahila, M. P. (2022) · Journal of Veterinary and Animal Sciences · doi:10.51966/jvas.2022.53.1.70-78 · Cited by 1 · see citing works
Joseph, T. C., Bibindas, K. S., Lakshmi, T. R., Eltholth, M. M., & Murray, F. J. (2021) · Research Square · doi:10.21203/rs.3.rs-740362/v1 · Cited by 1 · see citing works
Safety and Microbiological Quality
Remize, F., & Montēt, D. (2019) · Fermentation · doi:10.3390/fermentation5020050
Tan, Q., Zeng, Z., Xu, F., & Wei, H. (2021) · Canadian Journal of Infectious Diseases and Medical Microbiology · doi:10.1155/2021/6655414
“faecalis strains are regarded as commensal bacteria or even probiotics for creating a healthy gut environment; however, other strains are considered to be dangerous as they can lead to a variety of nosocomial infections and diseases [2, 3].”
Background
- Authors
- Baccouri, O., Boukerb, A. M., Farhat, L. B., Zébré, A., Zimmermann, K., Domann, E., Cambronel, M., Barreau, M., Maillot, O., Rincé, I., Muller, C., Marzouki, M. N., Feuilloley, M., Abidi, F., & Connil, N.
- Affiliations
- University of Carthage (TN)
- Normandie Université (FR)
- SymbioPharm (Germany) (DE)
- Justus-Liebig-Universität Gießen (DE)
- Corresponding author
- Connil, Nathalie
- ORCID
- Venue
- Frontiers in Microbiology
- Publisher
- Frontiers Media SA
- Published
- 2019-04-24
- Volume
- 10
- Pages
- 881-881
- ISSN
- 1664-302X (ISSN-L)
- PMC
- PMC6491886
- OpenAlex
- W2942463136
- Cited by
- 117
- References
- 92
- Citations by year
- 2026: 10 · 2025: 15 · 2024: 9 · 2023: 16 · 2022: 27 · 2021: 23 · 2020: 12 · 2019: 5
- FWCI
- 12.92
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Frontiers in Microbiology (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- HAL (submittedVersion, cc-by)
- HAL (submittedVersion, other-oa)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- European Commission
- Région Normandie
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Keywords
- Enterococcus faecalis, probiotic potential, probiotic safety, fermented dairy products, gastrointestinal tolerance, bile salt tolerance, biofilm formation, intestinal barrier, antibiotic susceptibility, tetracycline resistance, Galleria mellonella virulence, whole genome sequencing, average nucleotide identity
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
