Strenuous Acute Exercise Induces Slow and Fast Twitch-Dependent NADPH Oxidase Expression in Rat Skeletal Muscle

Osório Alves, Juliana; Matta Pereira, Leonardo; Cabral Coutinho do Rêgo Monteiro, Igor; Pontes dos Santos, Luiz Henrique; Soares Marreiros Ferraz, Alex; Carneiro Loureiro, Adriano Cesar; Calado Lima, Crystianne; Leal-Cardoso, José Henrique; Pires Carvalho, Denise; Soares Fortunato, Rodrigo; Marilande Ceccatto, Vânia

doi:10.3390/antiox9010057

Cited by 53Exercise and Physiological ResponsesCardiovascular and exercise physiologyMuscle Physiology and Disorders

Strenuous Acute Exercise Induces Slow and Fast Twitch-Dependent NADPH Oxidase Expression in Rat Skeletal Muscle

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Osório Alves, Juliana, Matta Pereira, Leonardo, Cabral Coutinho do Rêgo Monteiro, Igor, Pontes dos Santos, Luiz Henrique, Soares Marreiros Ferraz, Alex, Carneiro Loureiro, Adriano Cesar, Calado Lima, Crystianne, Leal-Cardoso, José Henrique +3 more·2020·Antioxidants

DOI 10.3390/antiox9010057PMC PMC7022445

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Abstract

The enzymatic complex Nicotinamide Adenine Dinucleotide Phosphate (NADPH) oxidase (NOx) may be the principal source of reactive oxygen species (ROS). The NOX2 and NOX4 isoforms are tissue-dependent and are differentially expressed in slow-twitch fibers (type I fibers) and fast-twitch fibers (type II fibers) of skeletal muscle, making them different markers of ROS metabolism induced by physical exercise. The aim of this study was to investigate NOx signaling, as a non-adaptive and non-cumulative response, in the predominant fiber types of rat skeletal muscles 24 h after one strenuous treadmill exercise session. The levels of mRNA, reduced glycogen, thiol content, NOx, superoxide dismutase, catalase, glutathione peroxidase activity, and PPARGC1α and SLC2A4 gene expression were measured in the white gastrocnemius (WG) portion, the red gastrocnemius (RG) portion, and the soleus muscle (SOL). NOx activity showed higher values in the SOL muscle compared to the RG and WG portions. The same was true of the NOX2 and NOX4 mRNA levels, antioxidant enzymatic activities, glycogen content. Twenty-four hours after the strenuous exercise session, NOx expression increased in slow-twitch oxidative fibers. The acute strenuous exercise condition showed an attenuation of oxidative stress and an upregulation of antioxidant activity through PPARGC1α gene activity, antioxidant defense adaptations, and differential gene expression according to the predominant fiber type. The most prominent location of detoxification (indicated by NOX4 activation) in the slow-twitch oxidative SOL muscle was the mitochondria, while the fast-twitch oxidative RG portion showed a more cytosolic location. Glycolytic metabolism in the WG portion suggested possible NOX2/NOX4 non-regulation, indicating other possible ROS regulation pathways.

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Skeletal muscle NOX2 expression is increased by acute exercise.
“Recent studies have shown that skeletal muscle NOX2 expression is increased by acute exercise (27)”
From Skeletal muscle NOX4 is required for adaptive responses that prevent insulin resistance (2021) · cites this paper as [27]

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Authors: Osório Alves, J., Matta Pereira, L., Cabral Coutinho do Rêgo Monteiro, I., Pontes dos Santos, L. H., Soares Marreiros Ferraz, A., Carneiro Loureiro, A. C., Calado Lima, C., Leal-Cardoso, J. H., Pires Carvalho, D., Soares Fortunato, R., & Marilande Ceccatto, V.
Affiliations: Laboratório de Expressão Gênica, Instituto Superior de Ciências Biomédicas, Universidade Estadual do Ceará, Fortaleza 60.714-903, Brazil
Laboratório de Fisiologia e Sinalização redox, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-902, Brazil
Instituto de Educação Física e Esportes, Universidade Federal do Ceará, Fortaleza 60455-760, Brazil
Laboratório de Eletrofisiologia Instituto Superior de Ciências Biomédicas, Universidade Estadual do Ceará, Fortaleza 60.714-903, Brazil
Laboratório de Fisiologia Endócrina Doris Rosenthal, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-902, Brazil
ORCID: Matta Pereira, Leonardo
Leal-Cardoso, José Henrique
Marilande Ceccatto, Vânia
Venue: Antioxidants
Publisher: MDPI AG
Published: 2020-01-08
Volume: 9
Issue: 1
Pages: 57
ISSN: 2076-3921 (ISSN-L)
DOI: 10.3390/antiox9010057
PMC: PMC7022445
OpenAlex: W2999055358
Cited by: 53
References: 61
Citations by year: 2026: 5 · 2025: 9 · 2024: 6 · 2023: 9 · 2022: 10 · 2021: 11 · 2020: 3
FWCI: 4.57
Type: journal article
Language: en
Open access: gold
dx.doi.org
Licence: cc-by
creativecommons.org/licenses/by/4.0/ (vor)
Locations: Antioxidants (publishedVersion, cc-by)
Europe PMC (PubMed Central) (publishedVersion, cc-by)
PubMed Central (publishedVersion, cc-by)
PubMed (publishedVersion)
LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (publishedVersion, other-oa)
DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Topics: Health Sciences › Medicine › Rehabilitation › Exercise and Physiological Responses
Health Sciences › Medicine › Complementary and alternative medicine › Cardiovascular and exercise physiology
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
Keywords: NADPH oxidase, NOX2, NOX4, reactive oxygen species, oxidative stress, skeletal muscle fiber types, type I muscle fibers, fast-twitch fibers, treadmill exercise, antioxidant enzymes, SLC2A4, soleus muscle, gastrocnemius, glycogen, thiol content, gene expression

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Strenuous Acute Exercise Induces Slow and Fast Twitch-Dependent NADPH Oxidase Expression in Rat Skeletal Muscle

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