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Cited by 43Proteoglycans and glycosaminoglycans researchGlycosylation and Glycoproteins ResearchCarbohydrate Chemistry and Synthesis

Reconsideration of the Semaphorin-3A Binding Motif Found in Chondroitin Sulfate Using Galnac4s-6st-Knockout Mice

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Nadanaka, Satomi, Miyata, Shinji, Yaqiang, Bai, Tamura, Jun-ichi, Habuchi, Osami, Kitagawa, Hiroshi·2020·Biomolecules
DOI 10.3390/biom10111499PMC PMC7694144
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Abstract

The chondroitin sulfate (CS)-rich dense extracellular matrix surrounding neuron cell bodies and proximal dendrites in a mesh-like structure is called a perineuronal net (PNN). CS chains in PNNs control neuronal plasticity by binding to PNN effectors, semaphorin-3A (Sema3A) and orthodenticle homeobox 2. Sema3A recognizes CS-containing type-E disaccharide units (sulfated at O-4 and O-6 of N-acetylgalactosamine). Type-E disaccharide units are synthesized by N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase (GalNAc4S-6ST). In this study, we demonstrated that Sema3A accumulates in the PNNs surrounding parvalbumin cells, even in mice deficient in GalNAc4S-6ST. In addition, there were no differences in the number and structure of PNNs visualized by Cat316 antibody and Wisteria floribunda lectin, which recognize CS chains, between wild type and GalNAc4S-6ST knockout mice. Therefore, we re-examined the Sema3A binding motif found in CS chains using chemically synthesized CS tetrasaccharides. As a result, we found that non-sulfated GalNAc residues at the non-reducing termini of CS chains are required for the binding of Sema3A.

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  • WFA lectin preferentially recognizes and binds to non-sulfated 0S-CS tetrasaccharide units compared to sulfated isomer variants.

    “A recent study published by Nadanaka et al. showed WFA lectin preferentially recognizes and binds to non-sulfated 0S-CS tetrasaccharide units compared to sulfated isomer variants (Nadanaka et al. 2020).”

    From Perineuronal net deglycosylation associates with tauopathy‐induced gliosis and neurodegeneration (2024) · cites this paper as Nadanaka et al. 2020

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Cited by

Sorted by citations

Authors
Nadanaka, S., Miyata, S., Yaqiang, B., Tamura, J.-I., Habuchi, O., & Kitagawa, H.
Affiliations
  • Kobe Pharmaceutical University (JP)
  • Tokyo University of Agriculture and Technology (JP)
  • Laboratory of Biochemistry, Kobe Pharmaceutical University, Kobe 658-8558, Japan
  • Tottori University (JP)
  • Aichi Medical University (JP)
Corresponding author
Kitagawa, Hiroshi
ORCID
Venue
Biomolecules
Publisher
MDPI AG
Published
2020-10-30
Volume
10
Issue
11
Pages
1499
ISSN
2218-273X (ISSN-L)
DOI
10.3390/biom10111499
PMC
PMC7694144
OpenAlex
W3095533124
Cited by
43
References
7
Citations by year
2026: 6 · 2025: 6 · 2024: 10 · 2023: 3 · 2022: 8 · 2021: 10
FWCI
2.62
Type
journal article
Language
en
Open access
Licence
Locations
  • Biomolecules (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
Ministry of Education, Culture, Sports, Science and Technology (JP20H03386, JP25460080, JP23110003)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Proteoglycans and glycosaminoglycans research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Glycosylation and Glycoproteins Research
  • Physical Sciences › Chemistry › Organic Chemistry › Carbohydrate Chemistry and Synthesis
Keywords
semaphorin 3A, chondroitin sulfate, perineuronal nets, extracellular matrix, neuroplasticity
MeSH terms
Animals, Chondroitin Sulfates, Dendrites, Extracellular Matrix, Humans, Membrane Glycoproteins, Neuronal Plasticity, Neurons, Protein Binding, Sulfotransferases, Mice, Knockout, Semaphorin-3A, Mice, Otx Transcription Factors

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