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Cited by 118Neuroscience and Neuropharmacology ResearchGenetics and Neurodevelopmental DisordersNeurogenesis and neuroplasticity mechanisms

Structural maturation of cortical perineuronal nets and their perforating synapses revealed by superresolution imaging

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Sigal, Yaron M., Bae, Haneui, Bogart, Luke J., Hensch, Takao K., Zhuang, Xiaowei·2019·Proceedings of the National Academy of Sciences
DOI 10.1073/pnas.1817222116PMC PMC6452715
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Abstract

Significance: Particular inhibitory neurons containing parvalbumin (PV) play a critical role in determining the time course by which experience shapes developing brain circuits. Embedded deep within cortical gray matter, extracellular matrices enwrapping PV-positive (PV+) cells have remained difficult to study partly due to the small length scale on which they are organized. To overcome such challenges, we used a superresolution fluorescence imaging approach to visualize these perineuronal nets (PNNs), a specialized form of the extracellular matrix critical for maintaining mature PV+ neuron function and their synaptic inputs. We quantified changes that define PNN structure and identified specific synaptic defects in a mouse model lacking MeCP2 , the causative gene for Rett syndrome, a regressive neurodevelopmental disorder.

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  • PNN has an indirect role over principal neurons via regulating synaptic plasticity and dendritic arborization of the interneuron.

    “PNN has an indirect role over principal neurons via regulating synaptic plasticity (Sigal et al., 2019) and dendritic arborization (Stolp et al., 2019) of the interneuron.”

    From Disrupted structural connectivity in ArcAβ mouse model of Aβ amyloidosis (2020) · cites this paper as Sigal et al., 2019

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Authors
Sigal, Y. M., Bae, H., Bogart, L. J., Hensch, T. K., & Zhuang, X.
Affiliations
  • Howard Hughes Medical Institute (US)
  • Harvard University (US)
  • Boston Children's Hospital (US)
ORCID
Venue
Proceedings of the National Academy of Sciences
Publisher
National Academy of Sciences
Published
2019-03-19
Volume
116
Issue
14
Pages
7071-7076
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
DOI
10.1073/pnas.1817222116
PMC
PMC6452715
OpenAlex
W2922660066
Cited by
118
References
11
Citations by year
2026: 13 · 2025: 14 · 2024: 13 · 2023: 15 · 2022: 21 · 2021: 20 · 2020: 16 · 2019: 6
FWCI
7.53
Type
journal article
Language
en
Open access
Licence
www.pnas.org/site/aboutpnas/licenses.xhtml (vor, 184 d)
Locations
  • Proceedings of the National Academy of Sciences (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • HHS | NIH | National Institute of Mental Health (P50MH094271)
  • Howard Hughes Medical Institute (HHMI)
  • HHS | NIH | National Institute of General Medical Sciences (R35GM122487)
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetics and Neurodevelopmental Disorders
  • Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
Keywords
perineuronal nets, parvalbumin interneurons, extracellular matrix, cortical development, MeCP2, Rett syndrome, inhibitory interneurons, synaptic defects, super-resolution imaging
MeSH terms
Animals, Male, Nerve Net, Neuronal Plasticity, Synapses, Visual Cortex, Rett Syndrome, Synaptotagmin II, Mice, Methyl-CpG-Binding Protein 2

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