The DOI matches its Crossref record; 41 of the 41 references it cites were found in a registry or an index.
Tangential Flow Filtration for Highly Efficient Concentration of Extracellular Vesicles from Large Volumes of Fluid
Open accessAbstract
Concentration of extracellular vesicles (EVs) from biological fluids in a scalable and reproducible manner represents a major challenge. This study reports the use of tangential flow filtration (TFF) for the highly efficient isolation of EVs from large volumes of samples. When compared to ultracentrifugation (UC), which is the most widely used method to concentrate EVs, TFF is a more efficient, scalable, and gentler method. Comparative assessment of TFF and UC of conditioned cell culture media revealed that the former concentrates EVs of comparable physicochemical characteristics, but with higher yield, less single macromolecules and aggregates (<15 nm in size), and improved batch-to-batch consistency in half the processing time (1 h). The TFF protocol was then successfully implemented on fluids derived from patient lipoaspirate. EVs from adipose tissue are of high clinical relevance, as they are expected to mirror the regenerative properties of the parent cells.
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Cites
Théry, C., Witwer, K. W., Aikawa, E., Alcaraz, M. J., Anderson, J. D., Andriantsitohaina, R., Antoniou, A., Arab, T., Archer, F., Atkin‐Smith, G. K., et al. (2018) · Journal of Extracellular Vesicles · doi:10.1080/20013078.2018.1535750 · Cited by 11,099
Self-citationBiogenesis, Secretion, and Intercellular Interactions of Exosomes and Other Extracellular Vesicles
Colombo, M., Raposo, G., & Théry, C. (2014) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev-cellbio-101512-122326 · Cited by 6,186
Witwer, K. W., Buzás, E. I., Bemis, L. T., Bora, A., Lässer, C., Lötvall, J., Nolte‐‘t Hoen, E. N., Piper, M. G., Sivaraman, S., Skog, J., et al. (2013) · Journal of Extracellular Vesicles · doi:10.3402/jev.v2i0.20360 · Cited by 2,421
Zhang, H., Freitas, D., Kim, H. S., Fabijanic, K., Li, Z., Chen, H., Mark, M. T., Molina, H., Martin, A. B., Bojmar, L., et al. (2018) · Nature Cell Biology · doi:10.1038/s41556-018-0040-4 · Cited by 1,613
EV-TRACK: transparent reporting and centralizing knowledge in extracellular vesicle research
EV-TRACK Consortium, Van Deun, J., Mestdagh, P., Agostinis, P., Akay, Ö., Anand, S., Anckaert, J., Martinez, Z. A., Baetens, T., Beghein, E., et al. (2017) · Nature Methods · doi:10.1038/nmeth.4185 · Cited by 1,284
Mesenchymal Stem Cell-derived Extracellular Vesicles: Toward Cell-free Therapeutic Applications
Rani, S., Ryan, A. E., Griffin, M. D., & Ritter, T. (2015) · Molecular Therapy · doi:10.1038/mt.2015.44 · Cited by 1,227
Gardiner, C., Di Vizio, D., Sahoo, S., Théry, C., Witwer, K. W., Wauben, M., & Hill, A. F. (2016) · Journal of Extracellular Vesicles · doi:10.3402/jev.v5.32945 · Cited by 1,069
Methodological Guidelines to Study Extracellular Vesicles
Coumans, F. A. W., Brisson, A. R., Buzas, E. I., Dignat-George, F., Drees, E. E. E., El-Andaloussi, S., Emanueli, C., Gasecka, A., Hendrix, A., Hill, A. F., et al. (2017) · Circulation Research · doi:10.1161/circresaha.117.309417 · Cited by 1,017
Baranyai, T., Herczeg, K., Onódi, Z., Voszka, I., Módos, K., Marton, N., Nagy, G., Mäger, I., Wood, M. J., El Andaloussi, S., et al. (2015) · PLOS ONE · doi:10.1371/journal.pone.0145686 · Cited by 670
Gámez-Valero, A., Monguió-Tortajada, M., Carreras-Planella, L., Franquesa, M., Beyer, K., & Borràs, F. E. (2016) · Scientific Reports · doi:10.1038/srep33641 · Cited by 594
Cited by
Welsh, J. A., Goberdhan, D. C. I., O'Driscoll, L., Buzas, E. I., Blenkiron, C., Bussolati, B., Cai, H., Di Vizio, D., Driedonks, T. A. P., Erdbrügger, U., et al. (2024) · Journal of Extracellular Vesicles · doi:10.1002/jev2.12404 · Cited by 4,663
Self-citationTargeting drugs to tumours using cell membrane-coated nanoparticles
Fang, R. H., Gao, W., & Zhang, L. (2023) · Nature Reviews Clinical Oncology · doi:10.1038/s41571-022-00699-x · Cited by 980
Extracellular miRNAs: From Biomarkers to Mediators of Physiology and Disease
Mori, M. A., Ludwig, R. G., Garcia-Martin, R., Brandão, B. B., & Kahn, C. R. (2019) · Cell Metabolism · doi:10.1016/j.cmet.2019.07.011 · Cited by 961
Exosome Processing and Characterization Approaches for Research and Technology Development
Lai, J. J., Chau, Z. L., Chen, S., Hill, J. J., Korpany, K. V., Liang, N., Lin, L., Lin, Y., Liu, J. K., Liu, Y., et al. (2022) · Advanced Science · doi:10.1002/advs.202103222 · Cited by 623
Review on Strategies and Technologies for Exosome Isolation and Purification
Chen, J., Li, P., Zhang, T., Xu, Z., Huang, X., Wang, R., & Du, L. (2022) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2021.811971 · Cited by 585
Metadata
- Authors
- Busatto, S., Vilanilam, G., Ticer, T., Lin, W.-L., Dickson, D. W., Shapiro, S., Bergese, P., & Wolfram, J.
- Venue
- Cells
- Publisher
- MDPI AG
- Published
- 2018-12-16
- Volume
- 7
- Issue
- 12
- Pages
- 273
- ISSN
- 2073-4409 (ISSN-L)
- OpenAlex
- W2904976916
- Cited by
- 532
- References
- 41
- FWCI
- 9.18
- Type
- journal article
- Language
- en
- Open access
- gold
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- Busatto, S., Vilanilam, G., Ticer, T., Lin, W.-L., Dickson, D. W., Shapiro, S., Bergese, P., & Wolfram, J. (2018). Tangential Flow Filtration for Highly Efficient Concentration of Extracellular Vesicles from Large Volumes of Fluid. Cells. https://doi.org/10.3390/cells7120273
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
