Biomass and Lipid Productivity by Two Algal Strains of Chlorella sorokiniana Grown in Hydrolysate of Water Hyacinth
Open accessPublished version of a preprint
Abstract
Hydrolysate prepared from the chemical hydrolysis of water hyacinth biomass contains a high amount of solubilised carbohydrate and nutrients. This hydrolysate was utilised as a medium for the cultivation of two strains of Chlorella sorokiniana, isolated from a municipal wastewater treatment plant using two different media, i.e., BG-11 and Knop’s medium. Different light intensities, light–dark cycles, and various concentrations of external carbon sources (monosaccharides and inorganic carbon) were used to optimise the microalgal growth. For the accumulation of lipids and carbohydrates, the microalgal strains were transferred to nutrient amended medium (N-amended and P-amended). It was observed that the combined effect of glucose, inorganic carbon, and a 12:12 h light–dark cycle proved to be the optimum parameters for high biomass productivity (~200 mg/L/day). For Chlorella sorokiniana 1 (isolated from BG-11 medium), the maximum carbohydrate content (22%) was found in P-amended medium (N = 0 mg/L, P: 3 mg/L), whereas, high lipid content (17.3%) was recorded in N-amended medium (N = 5 mg/L, P = 0 mg/L). However, for Chlorella sorokiniana 2 (isolated from the Knop’s medium), both lipid (17%) and carbohydrate accumulation (12.3%) were found to be maximum in the N-amended medium. Chlorella sorokiniana 2 showed a high saturated lipid accumulation compared to other strains. Kinetic modelling of the lipid profile revealed that the production rate of fatty acids and their various constituents were species dependent under identical conditions.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
A comparative assessment of the ability of microalgae to accumulate lipids without specifying information on cultivation media can only be indicative.
“A comparative assessment of the ability of microalgae to accumulate lipids without specifying information on cultivation media [13, 135] can only be indicative.”
From Nitrogen and phosphorus stress as a tool to induce lipid production in microalgae (2023) · cites this paper as [135]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Introduction to Linear Regression Analysis.
Assunção, R., Sampson, P. D., Montgomery, D. C., & Peck, E. A. (1993) · Journal of the American Statistical Association · doi:10.2307/2290746 · Cited by 5,389 · see citing works
Brennan, L., & Owende, P. (2010) · Renewable and Sustainable Energy Reviews · doi:10.1016/j.rser.2009.10.009 · Cited by 4,894 · see citing works
Unipro UGENE: a unified bioinformatics toolkit
Okonechnikov, K., Golosova, O., Fursov, M., & the UGENE team (2012) · Bioinformatics · doi:10.1093/bioinformatics/bts091 · Cited by 3,181 · see citing works
Lipid productivity as a key characteristic for choosing algal species for biodiesel production
Griffiths, M. J., & Harrison, S. T. L. (2009) · Journal of Applied Phycology · doi:10.1007/s10811-008-9392-7 · Cited by 1,421 · see citing works
‘Morphology, composition, production, processing and applications of Chlorella vulgaris: a review’.
Safi, C., Zebib, B., Merah, O., Pontalier, P.-Y., & Vaca-Garcia, C. (2014) · Renewable and Sustainable Energy Reviews · doi:10.1016/j.rser.2014.04.007 · Cited by 1,127 · see citing works
Li, Y., Horsman, M., Wang, B., Wu, N., & Lan, C. Q. (2008) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-008-1681-1 · Cited by 1,116 · see citing works
Effect of iron on growth and lipid accumulation in Chlorella vulgaris
Liu, Z., Wang, G., & Zhou, B. (2007) · Bioresource Technology · doi:10.1016/j.biortech.2007.09.073 · Cited by 815 · see citing works
Sajjadi, B., Chen, W.-Y., Raman, A. A. A., & Ibrahim, S. (2018) · Renewable and Sustainable Energy Reviews · doi:10.1016/j.rser.2018.07.050 · Cited by 535 · see citing works
Nitrogen availability influences phosphorus removal in microalgae-based wastewater treatment
Beuckels, A., Smolders, E., & Muylaert, K. (2015) · Water Research · doi:10.1016/j.watres.2015.03.018 · Cited by 351 · see citing works
Chen, B., Wan, C., Mehmood, M. A., Chang, J.-S., Bai, F., & Zhao, X. (2017) · Bioresource Technology · doi:10.1016/j.biortech.2017.05.170 · Cited by 346 · see citing works
Griffiths, M. J., van Hille, R. P., & Harrison, S. T. L. (2012) · Journal of Applied Phycology · doi:10.1007/s10811-011-9723-y · Cited by 341 · see citing works
Heredia-Arroyo, T., Wei, W., Ruan, R. R., & Hu, B. (2011) · Biomass and Bioenergy · doi:10.1016/j.biombioe.2011.02.036 · Cited by 326 · see citing works
Trends and novel strategies for enhancing lipid accumulation and quality in microalgae
Singh, P., Kumari, S., Guldhe, A., Misra, R., Rawat, I., & Bux, F. (2016) · Renewable and Sustainable Energy Reviews · doi:10.1016/j.rser.2015.11.001 · Cited by 309 · cited 1× · see citing works
“Various ions, such as iron and magnesium, are essential for cellular mechanisms, including photosynthesis, cell division, respiration, intracellular transportation, and protein synthesis in microalgae [23,24].”
BackgroundWang, H., Xiong, H., Hui, Z., & Zeng, X. (2011) · Bioresource Technology · doi:10.1016/j.biortech.2011.11.020 · Cited by 305 · see citing works
Bioremediation of textile wastewater and successive biodiesel production using microalgae
Fazal, T., Mushtaq, A., Rehman, F., Khan, A. U., Rashid, N., Farooq, W., Rehman, M. S. U., & Xu, J. H. (2017) · Renewable and Sustainable Energy Reviews · doi:10.1016/j.rser.2017.10.029 · Cited by 301 · see citing works
Zhan, J., Rong, J., & Wang, Q. (2016) · International Journal of Hydrogen Energy · doi:10.1016/j.ijhydene.2016.12.021 · Cited by 291 · see citing works
Light and growth medium effect on Chlorella vulgaris biomass production
Blair, M. F., Kokabian, B., & Gude, V. G. (2014) · Journal of Environmental Chemical Engineering · doi:10.1016/j.jece.2013.11.005 · Cited by 238 · see citing works
Lowrey, J., Brooks, M. S., & McGinn, P. J. (2015) · Journal of Applied Phycology · doi:10.1007/s10811-014-0459-3 · Cited by 226 · see citing works
Gao, F., Yang, H., Li, C., Peng, Y., Lu, M., Jin, W., Jing-jiao, B., & Guo, Y. (2019) · Bioresource Technology · doi:10.1016/j.biortech.2019.03.011 · Cited by 223 · see citing works
Roleda, M. Y., Slocombe, S. P., Leakey, R. J. G., Day, J. G., Bell, E. M., & Stanley, M. S. (2013) · Bioresource Technology · doi:10.1016/j.biortech.2012.11.043 · Cited by 222 · see citing works
Effect of nutrient supply status on biomass composition of eukaryotic green microalgae
Prochazková, G., Brányiková, I., Zachleder, V., & Brányik, T. (2013) · Journal of Applied Phycology · doi:10.1007/s10811-013-0154-9 · Cited by 221 · see citing works
Chu, F., Chu, P.-N., Cai, P.-J., Li, W., Lam, P. K. S., & Zeng, R. J. (2013) · Bioresource Technology · doi:10.1016/j.biortech.2013.01.131 · Cited by 211 · see citing works
Feng, D., Chen, Z., Xue, S., & Zhang, W. Y. (2011) · Bioresource Technology · doi:10.1016/j.biortech.2011.04.006 · Cited by 203 · see citing works
Singh, P., Guldhe, A., Kumari, S., Rawat, I., & Bux, F. (2015) · Biochemical Engineering Journal · doi:10.1016/j.bej.2014.10.019 · Cited by 202 · see citing works
Improving photosynthesis for algal biofuels: toward a green revolution
Stephenson, P. G., Moore, C. M., Terry, M. J., Zubkov, M. V., & Bibby, T. S. (2011) · Trends in biotechnology · doi:10.1016/j.tibtech.2011.06.005 · Cited by 197 · see citing works
Microalgal feeds for aquaculture
Gladue, R. M., & Maxey, J. E. (1994) · Journal of Applied Phycology · doi:10.1007/bf02186067 · Cited by 172 · see citing works
Lin, T.-S., & Wu, J.-Y. (2015) · Bioresource Technology · doi:10.1016/j.biortech.2014.11.005 · Cited by 170 · see citing works
Strategies to enhance production of microalgal biomass and lipids for biofuel feedstock
Chu, W.-L. (2017) · European Journal of Phycology · doi:10.1080/09670262.2017.1379100 · Cited by 146 · see citing works
Castro, Y. A., Ellis, J. T., Miller, C., & Sims, R. C. (2014) · Applied Energy · doi:10.1016/j.apenergy.2014.11.045 · Cited by 142 · see citing works
Multilateral Approach on Enhancing Economic Viability of Lipid Production from Microalgae: A Review
Shin, Y. S., Choi, H. I., Choi, J. W., Lee, J. S., Sung, Y. J., & Sim, S. J. (2018) · Bioresource Technology · doi:10.1016/j.biortech.2018.03.002 · Cited by 122 · see citing works
Li, X., Li, W., Zhai, J., & Wei, H. (2018) · Bioresource Technology · doi:10.1016/j.biortech.2018.05.046 · Cited by 119 · see citing works
Effect of physical pretreatment on dilute acid hydrolysis of water hyacinth (Eichhornia crassipes)
Harun, M. Y., Biak, D. R. A., Abidin, Z. Z., & Yunus, R. B. (2011) · Bioresource Technology · doi:10.1016/j.biortech.2011.02.001 · Cited by 118 · see citing works
Rai, M. P., Nigam, S., & Sharma, R. (2013) · Biomass and Bioenergy · doi:10.1016/j.biombioe.2013.08.038 · Cited by 111 · see citing works
Wan, M., Jin, X., Xia, J., Rosenberg, J. N., Yu, G., Nie, Z., Oyler, G. A., & Betenbaugh, M. J. (2014) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-014-6088-6 · Cited by 104 · see citing works
Song, M., & Pei, H. (2018) · Bioresource Technology · doi:10.1016/j.biortech.2018.05.020 · Cited by 102 · see citing works
Optimized inorganic carbon regime for enhanced growth and lipid accumulation in Chlorella vulgaris
Lohman, E. J., Gardner, R. D., Pedersen, T. C., Peyton, B., Cooksey, K. E., & Gerlach, R. (2015) · Biotechnology for Biofuels · doi:10.1186/s13068-015-0265-4 · Cited by 99 · see citing works
Fu, L., Cui, X., Li, Y., Xu, L., Zhang, C., Xiong, R., Zhou, D., & Crittenden, J. C. (2017) · Chemical Engineering Journal · doi:10.1016/j.cej.2017.07.182 · Cited by 94 · see citing works
Barua, V. B., & Kalamdhad, A. S. (2016) · Bioresource Technology · doi:10.1016/j.biortech.2016.12.036 · Cited by 93 · see citing works
Carney, L. T., Reinsch, S. S., Lane, P. D., Solberg, O. D., Jansen, L., Williams, K. P., Trent, J. D., & Lane, T. W. (2014) · Algal Research · doi:10.1016/j.algal.2013.11.006 · Cited by 91 · see citing works
Ren, L., Wang, P., Wang, C., Chen, J., Hou, J., & Qian, J. (2016) · Environmental Pollution · doi:10.1016/j.envpol.2016.09.061 · Cited by 87 · see citing works
Microalgas, produtos e aplicações
Dérner, R. B., Ohse, S., Braga, M. V. D. C., de Carvalho, S. M., & Fett, R. (2006) · Ciência Rural · doi:10.1590/s0103-84782006000600050 · Cited by 86 · see citing works
Pretreatment and enzymic saccharification of water hyacinth cellulose
ABDEL-FATTAH, A. F., & Abdel–Naby, M. A. (2011) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2011.10.033 · Cited by 85 · see citing works
Zhao, X., Tan, X., Yang, L., Liao, J.-Y., & Li, X. (2019) · Bioresource Technology · doi:10.1016/j.biortech.2019.03.117 · Cited by 85 · see citing works
Zhu, S., Wang, Y., Xu, J. H., Shang, C., Wang, Z., Xu, J., & Yuan, Z. (2015) · Bioresource Technology · doi:10.1016/j.biortech.2015.08.142 · Cited by 84 · see citing works
Deng, X., Chen, B., Xue, C., Li, D., Hu, X., & Gao, K. (2018) · Bioresource Technology · doi:10.1016/j.biortech.2018.11.032 · Cited by 84 · see citing works
de Alva, M. S., Luna‐Pabello, V. M., Ledesma, M. T. O., & Gómez, M. J. C. (2018) · Algal Research · doi:10.1016/j.algal.2018.07.006 · Cited by 74 · see citing works
Liu, T., Li, Y., Liu, F., & Wang, C. (2016) · Bioresource Technology · doi:10.1016/j.biortech.2015.12.021 · Cited by 72 · see citing works
Comparison of Autotrophic and Mixotrophic Cultivation of Green Microalgal for Biodiesel Production
Li, Y., Tsai, W., Hsu, Y.-C., Xie, M.-Z., & Chen, J. (2014) · Energy Procedia · doi:10.1016/j.egypro.2014.07.088 · Cited by 67 · see citing works
Synergetic effect of dilute acid and alkali treatments on fractional application of rice straw
Sun, S., Sun, S., Chen, W., et al. (2016) · Biotechnology for Biofuels · doi:10.1186/s13068-016-0632-9 · Cited by 62 · see citing works
Study of Chemical and Enzymatic Hydrolysis of Cellulosic Material to Obtain Fermentable Sugars
Amezcua‐Allieri, M. A., Durán, T. S., & Aburto, J. (2017) · Journal of Chemistry · doi:10.1155/2017/5680105 · Cited by 60 · see citing works
Huang, Y., Luo, L. X., Xu, K., & Wang, X. C. (2019) · Bioresource Technology · doi:10.1016/j.biortech.2019.121887 · Cited by 60 · see citing works
Pegallapati, A. K., & Nirmalakhandan, N. N. (2012) · Renewable Energy · doi:10.1016/j.renene.2012.09.052 · Cited by 59 · see citing works
Pleißner, D., Lau, K. Y., & Lin, C. S. K. (2016) · Journal of Cleaner Production · doi:10.1016/j.jclepro.2016.11.165 · Cited by 58 · see citing works
Maximizing Algal Growth in Batch Reactors Using Sequential Change in Light Intensity
Wahal, S., & Viamajala, S. (2010) · Applied Biochemistry and Biotechnology · doi:10.1007/s12010-009-8891-6 · Cited by 57 · see citing works
Tran, H., Kwon, J.-S., Kim, Z., Oh, Y., & Lee, C. (2010) · Biotechnology and Bioprocess Engineering · doi:10.1007/s12257-009-0127-7 · Cited by 57 · see citing works
Chai, S., Shi, J., Huang, T., Guo, Y., Wei, J., Guo, M., Li, L., Dou, S., Liu, L., & Liu, G. (2018) · PLoS ONE · doi:10.1371/journal.pone.0199873 · Cited by 56 · see citing works
Li, Y., Xu, H., Han, F., Mu, J., Chen, D., Feng, B., & Zeng, H. (2014) · Bioresource Technology · doi:10.1016/j.biortech.2014.07.028 · Cited by 52 · see citing works
An-yue, L. I. U., Chen, W., Zheng, L., & Song, L. (2011) · Progress in Natural Science Materials International · doi:10.1016/s1002-0071(12)60057-4 · Cited by 51 · see citing works
Hydrolysate of lipid extracted microalgal biomass residue: An algal growth promoter and enhancer
Maurya, R., Paliwal, C., Chokshi, K., Pancha, I., Ghosh, T., Satpati, G. G., Pal, R., Ghosh, A., & Mishra, S. (2016) · Bioresource Technology · doi:10.1016/j.biortech.2016.02.018 · Cited by 49 · see citing works
Effect of operating conditions on Chlorococcum sp. growth and lipid production
Aravantinou, A. F., & Manariotis, I. D. (2016) · Journal of environmental chemical engineering · doi:10.1016/j.jece.2016.01.028 · Cited by 45 · see citing works
Chowdhury, R., & Freire, F. M. C. S. (2015) · Applied Energy · doi:10.1016/j.apenergy.2015.05.045 · Cited by 41 · see citing works
Self-citationStrain variation in microalgal lipid production during mixotrophic growth with glycerol
Paranjape, K., Leite, G. B., & Hallenbeck, P. C. (2015) · Bioresource Technology · doi:10.1016/j.biortech.2015.12.071 · Cited by 38 · see citing works
Gu, H., Nagle, N. J., Pienkos, P. T., & Posewitz, M. C. (2014) · Bioresource Technology · doi:10.1016/j.biortech.2014.11.095 · Cited by 37 · see citing works
Woodworth, B. D., Mead, R. L., Nichols, C. N., & Kolling, D. R. J. (2014) · Bioresource Technology · doi:10.1016/j.biortech.2014.11.098 · Cited by 34 · see citing works
Moon, M., Kim, C. W., Farooq, W., Suh, W. I., Shrivastav, A., Park, M., Mishra, S. K., & Yang, J. (2014) · Bioresource Technology · doi:10.1016/j.biortech.2014.04.033 · Cited by 34 · see citing works
A SPECIAL EFFECT OF LIGHT ON THE GROWTH OF CHLORELLA VULGARIS
Killam, A. P., & Myers, J. E. (1956) · American Journal of Botany · doi:10.1002/j.1537-2197.1956.tb10535.x · Cited by 33 · see citing works
Li, Y., Yuan, Z., Mu, J., Chen, D., & Feng, B. (2013) · Energy & Fuels · doi:10.1021/ef4000177 · Cited by 32 · see citing works
Utilization of Xylose for Growth by the Eukaryotic Alga, Chlorella
Hawkins, R. L. (1999) · Current Microbiology · doi:10.1007/pl00006817 · Cited by 29 · see citing works
Algal growth in photo-bioreactors: Impact of illumination strategy and nutrient availability
Shriwastav, A., & Bose, P. (2015) · Ecological Engineering · doi:10.1016/j.ecoleng.2015.01.034 · Cited by 29 · see citing works
Yoon, S. Y., Hong, M. E., Chang, W.-S., et al. (2015) · Bioprocess and Biosystems Engineering · doi:10.1007/s00449-015-1383-x · Cited by 28 · see citing works
Chowdhury, R., Keen, P. L., & Tao, W. (2019) · Bioresource Technology Reports · doi:10.1016/j.biteb.2019.03.010 · Cited by 28 · see citing works
Self-citationMetabolism Part II: The tricarboxylic acid (TCA), citric acid, or Krebs cycle
Bodner, G. M. (1986) · Journal of Chemical Education · doi:10.1021/ed063p673 · Cited by 27 · see citing works
Characteristics of lipid biosynthesis of Chlorella pyrenoidosa under stress conditions
Dong, L., Li, D., & Li, C. (2020) · Bioprocess and Biosystems Engineering · doi:10.1007/s00449-020-02284-x · Cited by 25 · see citing works
Li, T., Wang, W., Yuan, C., Zhang, Y., Xu, J. H., Zheng, H., Xiang, W., & Li, A. (2020) · Journal of Applied Phycology · doi:10.1007/s10811-020-02056-w · Cited by 24 · see citing works
Rani, S., Chowdhury, R., Tao, W., & Srinivasan, A. (2019) · Chemistry and Ecology · doi:10.1080/02757540.2019.1688307 · Cited by 22 · see citing works
Self-citationLife cycle energy demand from algal biofuel generated from nutrients present in the dairy waste
Chowdhury, R., & Franchetti, M. J. (2016) · Sustainable Production and Consumption · doi:10.1016/j.spc.2016.07.001 · Cited by 20 · see citing works
Self-citationRibeiro, D. M., Zanetti, G. T., Julião, M. H. M., Masetto, T. E., Gelinski, J. M. L. N., & Fonseca, G. G. (2019) · Journal of Applied Biology & Biotechnology · doi:10.7324/jabb.2019.70102 · Cited by 18 · see citing works
Valdez-Ojeda, R. A., Serrano-Vázquez, M. G. D. R., Toledano-Thompson, T., et al. (2020) · BioEnergy Research · doi:10.1007/s12155-020-10160-5 · Cited by 13 · see citing works
Shi, M., Wei, H., Chen, Q., Wang, X., Zhou, W., & Liu, J. (2018) · Journal of Applied Phycology · doi:10.1007/s10811-018-1653-5 · Cited by 11 · see citing works
Dong, X., Huang, L., Li, T., Xu, J., Zhao, P., & Yu, X. (2019) · Energy Sources Part A Recovery Utilization and Environmental Effects · doi:10.1080/15567036.2019.1602227 · Cited by 10 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Nitrogen and phosphorus stress as a tool to induce lipid production in microalgae
Maltsev, Y., Kulikovskiy, M., & Maltseva, S. (2023) · Microbial Cell Factories · doi:10.1186/s12934-023-02244-6 · Cited by 174 · see citing works
“A comparative assessment of the ability of microalgae to accumulate lipids without specifying information on cultivation media [13, 135] can only be indicative.”
Background
- Authors
- Dahiya, S., Chowdhury, R., Tao, W., & Kumar, P.
- Affiliations
- Indian Institute of Technology Roorkee (IN)
- SUNY College of Environmental Science and Forestry (US)
- Sharda University (IN)
- Corresponding author
- Chowdhury, Raja
- Venue
- Energies
- Publisher
- MDPI AG
- Published
- 2021-03-04
- Volume
- 14
- Issue
- 5
- Pages
- 1411
- ISSN
- 1996-1073 (ISSN-L)
- OpenAlex
- W3133691504
- Cited by
- 19
- References
- 80
- Citations by year
- 2026: 2 · 2025: 5 · 2024: 3 · 2023: 5 · 2022: 2 · 2021: 2
- FWCI
- 0.56
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Energies (publishedVersion, cc-by)
- Preprints.org (acceptedVersion, cc-by)
- RePEc: Research Papers in Economics (submittedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Ministry of Human resource Development (DST-1589-CED, DBT-893-CED)
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Physical Sciences › Environmental Science › Environmental Chemistry › Aquatic Ecosystems and Phytoplankton Dynamics
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Aquaculture Nutrition and Growth
- Keywords
- Chlorella sorokiniana, microalgae cultivation, lipid accumulation, carbohydrate accumulation, biomass productivity, light-dark cycle, glucose supplementation, inorganic carbon, nutrient amendment, saturated fatty acids
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
