Lactobacillus plantarum DR7 modulated bowel movement and gut microbiota associated with dopamine and serotonin pathways in stressed adults
Open accessAbstract
We have previously reported that the administration of Lactobacillus plantarum DR7 for 12 weeks reduced stress and anxiety in stressed adults as compared to the placebo group, in association with changes along the brain neurotransmitters pathways of serotonin and dopamine-norepinephrine. We now aim to evaluate the effects of DR7 on gut functions, gut microbiota compositional changes, and determine the correlations between microbiota changes and the pathways of brain neurotransmitters. The administration of DR7 prevented an increase of defecation frequency over 12 weeks as compared to the placebo (p = 0.044), modulating the increase of stress-induced bowel movement. Over 12 weeks, alpha diversity of gut microbiota was higher in DR7 than the placebo group across class (p = 0.005) and order (p = 0.018) levels, while beta diversity differed between groups at class and order levels (p < 0.001). Differences in specific bacterial groups were identified, showing consistency at different taxonomic levels that survived multiplicity correction, along the phyla of Bacteroides and Firmicutes and along the classes of Deltaproteobacteria and Actinobacteria. Bacteroidetes, Bacteroidia, and Bacteroidales which were reduced in abundance in the placebo group showed opposing correlation with gene expression of dopamine beta hydrolase (DBH, dopamine pathway; p < 0.001), while Bacteroidia and Bacteroidales showed correlation with tryptophan hydroxylase-II (TPH2, serotonin pathway; p = 0.001). A correlation was observed between DBH and Firmicutes (p = 0.002), Clostridia (p < 0.001), Clostridiales (p = 0.001), Blautia (p < 0.001), and Romboutsia (p < 0.001), which were increased in abundance in the placebo group. Blautia was also associated with TDO (p = 0.001), whereas Romboutsia had an opposing correlation with TPH2 (p < 0.001). Deltaproteobacteria and Desulfovibrionales which were decreased in abundance in the placebo group showed opposing correlation with DBH (p = 0.001), whereas Bilophila was associated with TPH2 (p = 0.001). Our present data showed that physiological changes induced by L. plantarum DR7 could be associated with changes in specific taxa of the gut microbiota along the serotonin and dopamine pathways.
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Mechanistic studies have linked the stress response, cognition, and microbiota-gut-brain axis, demonstrating a relative abundance of fibre-degrading bacteria that produce short-chain fatty acids, as well as changes in the serotonin and dopamine-norepinephrine pathways.
“Mechanistic studies have further linked the stress response, cognition, and MGB axis, demonstrating a relative abundance of fibre-degrading bacteria that produce SCFAs, as well as changes in the serotonin and dopamine-norepinephrine pathways (474, 475).”
From Signalling cognition: The gut microbiota and hypothalamic-pituitary-adrenal axis (2023) · cites this paper as [475]
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Signalling cognition: The gut microbiota and hypothalamic-pituitary-adrenal axis
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Strain-Specific Therapeutic Potential of Lactiplantibacillus plantarum: A Systematic Scoping Review
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- Authors
- Liu, G., Chong, H.-X., Chung, F. Y.-L., Li, Y., & Liong, M.-T.
- Affiliations
- Chinese Academy of Sciences (CN)
- Universiti Sains Malaysia (MY)
- Corresponding author
- Li, Yin
- Liong, Min-Tze
- Venue
- International Journal of Molecular Sciences
- Publisher
- MDPI AG
- Published
- 2020-06-29
- Volume
- 21
- Issue
- 13
- Pages
- 4608
- ISSN
- 1422-0067 (ISSN-L)
- PMC
- PMC7370301
- OpenAlex
- W3037416159
- Cited by
- 83
- References
- 55
- Citations by year
- 2026: 14 · 2025: 12 · 2024: 11 · 2023: 19 · 2022: 15 · 2021: 11 · 2020: 1
- FWCI
- 7.46
- Type
- journal article
- Language
- en
- Open access
- gold
- dx.doi.org
- Licence
- Locations
- International Journal of Molecular Sciences (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Clinical Nutrition (In kind)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Health Sciences › Medicine › Gastroenterology › Gastrointestinal motility and disorders
- Keywords
- probiotics, stress, anxiety, gut microbiota, defecation, stool frequency, dopaminergic pathways, serotonin pathway, tryptophan metabolism, dopamine beta-hydroxylase, tryptophan dioxygenase, Bacteroidetes, Firmicutes, Deltaproteobacteria, Blautia, Romboutsia, Bilophila, gut-brain axis, alpha diversity
- MeSH terms
- Gastrointestinal Microbiome, Adult, Defecation, Dopamine, Double-Blind Method, Female, Humans, Male, Middle Aged, Serotonin, Stress, Psychological, Neurotransmitter Agents, Probiotics, Lactobacillus plantarum, Lactiplantibacillus plantarum
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