Cited by 19Genetic and phenotypic traits in livestockEffects of Environmental Stressors on LivestockGenetic Mapping and Diversity in Plants and Animals

Whole-Genome Resequencing Points to Candidate DNA Loci Affecting Body Temperature under Cold Stress in Siberian Cattle Populations

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Igoshin, Alexander, Yudin, Nikolay, Aitnazarov, Ruslan, Yurchenko, Andrey A., Larkin, Denis M.·2021·Life

Abstract

Despite the economic importance of creating cold resilient cattle breeds, our knowledge of the genetic basis of adaptation to cold environments in cattle is still scarce compared to information on other economically important traits. Herein, using whole-genome resequencing of animals showing contrasting phenotypes on temperature maintenance under acute cold stress combined with the existing SNP (single nucleotide polymorphism) functional annotations, we report chromosomal regions and candidate SNPs controlling body temperature in the Siberian cattle populations. The SNP ranking procedure based on regional FST calculations, functional annotations, and the allele frequency difference between cold-tolerant and cold-sensitive groups of animals pointed to multiple candidate genes. Among these, GRIA4, COX17, MAATS1, UPK1B, IFNGR1, DDX23, PPT1, THBS1, CCL5, ATF1, PLA1A, PRKAG1, and NR1I2 were previously related to thermal adaptations in cattle. Other genes, for example KMT2D and SNRPA1, are known to be related to thermogenesis in mice and cold adaptation in common carp, respectively. This work could be useful for cattle breeding strategies in countries with harsh climates, including the Russian Federation.

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  • Genes related to cold/heat stress response (DDX23, PPT1) and thermogenesis (GRIA4, HBS1) were found to be under selection.

    “Additionally, genes related to cold/heat stress response (DDX23, PPT1) and thermogenesis (GRIA4, HBS1) were found to be under selection [90].”

    From The Genomic Landscape of Cattle: Domestication, Dispersal, and Adaptive Evolution (2026) · cites this paper as [90]

    The Discussion (page unavailable) identifies DDX23 and GRIA4 among top-ranked candidate SNP results and links THBS1—not HBS1—to a pathway involved in adaptive thermogenesis. It does not identify GRIA4 as a thermogenesis gene or establish that these genes were under selection; its evidence is a ranking of candidate variants associated with temperature maintenance. The retrieved non-abstract passages do not establish the stated cold/heat-stress role for PPT1. (LLM, conf=0.88)

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Authors
Igoshin, A., Yudin, N., Aitnazarov, R., Yurchenko, A. A., & Larkin, D. M.
Affiliations
  • Institute of Cytology and Genetics (RU)
  • Novosibirsk State University (RU)
  • Institute of Cytology and Genetics SB RAS, 630090 Novosibirsk, Russia
  • University of London (GB)
Corresponding author
Larkin, Denis M.
Venue
Life
Publisher
MDPI AG
Published
2021-09-13
Volume
11
Issue
9
Pages
959
ISSN
2075-1729 (ISSN-L)
OpenAlex
W3199336323
Cited by
19
References
55
Citations by year
2026: 4 · 2025: 4 · 2024: 2 · 2023: 4 · 2022: 2 · 2021: 3
FWCI
1.55
Type
journal article
Language
en
Open access
Locations
  • Life (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, other-oa)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
Russian Science Foundation (19-76-20026)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic and phenotypic traits in livestock
  • Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Effects of Environmental Stressors on Livestock
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic Mapping and Diversity in Plants and Animals
Keywords
cold stress, body temperature, cold tolerance, thermal adaptation, candidate SNPs, whole-genome resequencing, FST, thermogenesis, KMT2D, cattle breeding, Russia

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