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Human-Scale Whole-Organ Bioengineering for Liver Transplantation: A Regenerative Medicine Approach
Open accessAbstract
At this time, the only definitive treatment of hepatic failure is liver transplantation. However, transplantation has been limited by the severely limited supply of human donor livers. Alternatively, a regenerative medicine approach has been recently proposed in rodents that describe the production of three-dimensional whole-organ scaffolds for assembly of engineered complete organs. In the present study, we describe the decellularization of porcine livers to generate liver constructs at a scale that can be clinically relevant. Adult ischemic porcine livers were successfully decellularized using a customized perfusion protocol, the decellularization process preserved the ultrastructural extracellular matrix components, functional characteristics of the native microvascular and the bile drainage network of the liver, and growth factors necessary for angiogenesis and liver regeneration. Furthermore, isolated hepatocytes engrafted and reorganized in the porcine decellularized livers using a human-sized organ culture system. These results provide proof-of-principle for the generation of a human-sized, three-dimensional organ scaffold as a potential structure for human liver grafts reconstruction for transplantation to treat liver disease.
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“Furthermore, they observed that albumin immunostaining was lower after 7 days of media perfusion compared to day 4, attributed to shear stress caused by media flow.”
From Liver tissue engineering using decellularized scaffolds: Current progress, challenges, and opportunities (2024) · cites this paper as [198]
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“Fortunately, the biliary network tree is confirmed to be intact after decellularization by scan electron microscopy, corrosion cast, dye perfusion, and contrast radiography; only reconstruction by bile duct cells is necessary [12,110,170,198].”
From Liver tissue engineering using decellularized scaffolds: Current progress, challenges, and opportunities (2024) · cites this paper as [198]
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“Importantly, they reported that around 47.8 % of reseeded hepatocytes were apoptotic by day 7.”
From Liver tissue engineering using decellularized scaffolds: Current progress, challenges, and opportunities (2024) · cites this paper as [198]
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An overview of tissue and whole organ decellularization processes
Crapo, P. M., Gilbert, T. W., & Badylak, S. F. (2011) · Biomaterials · doi:10.1016/j.biomaterials.2011.01.057 · Cited by 3,591
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Ott, H. C., Matthiesen, T. S., Goh, S.-K., Black, L. D., Kren, S. M., Netoff, T. I., & Taylor, D. A. (2008) · Nature Medicine · doi:10.1038/nm1684 · Cited by 2,650
Extracellular matrix as a biological scaffold material: Structure and function
BADYLAK, S., FREYTES, D., & GILBERT, T. (2009) · Acta Biomaterialia · doi:10.1016/j.actbio.2008.09.013 · Cited by 1,671
Self-citationUygun, B. E., Soto-Gutierrez, A., Yagi, H., Izamis, M.-L., Guzzardi, M. A., Shulman, C., Milwid, J., Kobayashi, N., Tilles, A., Berthiaume, F., et al. (2010) · Nature Medicine · doi:10.1038/nm.2170 · Cited by 1,370
Self-citationReprint of: Extracellular matrix as a biological scaffold material: Structure and function
Badylak, S. F., Freytes, D. O., & Gilbert, T. W. (2015) · Acta Biomaterialia · doi:10.1016/j.actbio.2015.07.016 · Cited by 1,367
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Regeneration and orthotopic transplantation of a bioartificial lung
Ott, H. C., Clippinger, B., Conrad, C., Schuetz, C., Pomerantseva, I., Ikonomou, L., Kotton, D., & Vacanti, J. P. (2010) · Nature Medicine · doi:10.1038/nm.2193 · Cited by 1,090
Davis, G. L., Alter, M. J., El‐Serag, H. B., Poynard, T., & Jennings, L. W. (2009) · Gastroenterology · doi:10.1053/j.gastro.2009.09.067 · Cited by 879
Hepatocyte function and extracellular matrix geometry: long‐term culture in a sandwich configuration
Dunn, J., Yarmush, M. L., Koebe, H. G., & Tompkins, R. G. (1989) · The FASEB Journal · doi:10.1096/fasebj.3.2.2914628 · Cited by 730
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Park, K.-M., Hussein, K. H., Hong, S.-H., Ahn, C., Yang, S.-R., Park, S.-M., Kweon, O.-K., Kim, B.-M., & Woo, H.-M. (2016) · Tissue Engineering Part A · doi:10.1089/ten.tea.2015.0313 · Cited by 113
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Verstegen, M. M. A., Willemse, J., van den Hoek, S., Kremers, G., Luider, T. M., van Huizen, N. A., Willemssen, F. E. J. A., Metselaar, H. J., IJzermans, J. N. M., van der Laan, L. J. W., et al. (2017) · Stem Cells and Development · doi:10.1089/scd.2017.0095 · Cited by 94
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Damodaran, R. G., & Vermette, P. (2018) · Biotechnology Progress · doi:10.1002/btpr.2699 · Cited by 91
Sasaki, K., Akagi, T., Asaoka, T., Eguchi, H., Fukuda, Y., Iwagami, Y., Yamada, D., Noda, T., Wada, H., Gotoh, K., et al. (2017) · Biomaterials · doi:10.1016/j.biomaterials.2017.02.034 · Cited by 90
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Shaheen, M. F., Joo, D. J., Ross, J. J., Anderson, B. D., Chen, H. S., Huebert, R. C., Li, Y., Amiot, B., Young, A., Zlochiver, V., et al. (2019) · Nature Biomedical Engineering · doi:10.1038/s41551-019-0460-x · Cited by 75
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Struecker, B., Hillebrandt, K. H., Voitl, R., Butter, A., Schmuck, R. B., Reutzel‐Selke, A., Geisel, D., Joehrens, K., Pickerodt, P. A., Raschzok, N., et al. (2014) · Tissue Engineering Part C Methods · doi:10.1089/ten.tec.2014.0321 · Cited by 74
Zhou, P., Huang, Y., Guo, Y., Wang, L., Ling, C., Guo, Q., Wang, Y., Zhu, S., Fan, X., Zhu, M., et al. (2016) · Artificial Organs · doi:10.1111/aor.12645 · Cited by 73
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Wu, Q., Bao, J., Zhou, Y., Wang, Y., Du, Z., Shi, Y., Li, L., & Bu, H. (2015) · BioMed Research International · doi:10.1155/2015/785474 · Cited by 67
Struecker, B., Butter, A., Hillebrandt, K., Polenz, D., Reutzel-Selke, A., Tang, P., Lippert, S., Leder, A., Rohn, S., Geisel, D., et al. (2017) · Journal of Tissue Engineering and Regenerative Medicine · doi:10.1002/term.1948 · Cited by 65
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Kawasaki, T., Kirita, Y., Kami, D., Kitani, T., Ozaki, C., Itakura, Y., Toyoda, M., & Gojo, S. (2015) · Journal of Biomedical Materials Research Part A · doi:10.1002/jbm.a.35474 · Cited by 61
Zheng, C., Sui, B., Hu, C., Qiu, X., Zhao, P., & Jin, Y. J. (2018) · Journal of Tissue Engineering and Regenerative Medicine · doi:10.1002/term.2676 · Cited by 62
Ogiso, S., Yasuchika, K., Fukumitsu, K., Ishii, T., Kojima, H., Miyauchi, Y., Yamaoka, R., Komori, J., Katayama, H., Kawai, T., et al. (2016) · Scientific Reports · doi:10.1038/srep35887 · Cited by 59
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Kadota, Y., Yagi, H., Inomata, K., Matsubara, K., Hibi, T., Abe, Y., Kitago, M., Shinoda, M., Obara, H., Itano, O., et al. (2014) · Organogenesis · doi:10.4161/org.27879 · Cited by 58
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Croce, S., Peloso, A., Zoro, T., Avanzini, M. A., & Cobianchi, L. (2019) · Biomolecules · doi:10.3390/biom9120813 · Cited by 59
Zhang, J., Chan, H. F., Wang, H., Shao, D., Tao, Y., & Li, M. (2021) · Journal of Tissue Engineering · doi:10.1177/2041731420986711 · Cited by 55
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Bühler, N. E. M., Schulze-Osthoff, K., Königsrainer, A., & Schenk, M. (2015) · Journal of Bioscience and Bioengineering · doi:10.1016/j.jbiosc.2014.10.019 · Cited by 51
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Orlando, G., Söker, S., Stratta, R. J., & Atala, A. (2013) · Cold Spring Harbor Perspectives in Medicine · doi:10.1101/cshperspect.a015693 · Cited by 49
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Rashtbar, M., Hadjati, J., Ai, J., Jahanzad, I., Azami, M., Shirian, S., Ebrahimi‐Barough, S., & Sadroddiny, E. (2017) · Journal of Biomedical Materials Research Part B Applied Biomaterials · doi:10.1002/jbm.b.33899 · Cited by 36
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Ward, A., Klassen, D. K., Franz, K. M., Giwa, S. E., & Lewis, J. K. (2018) · Current Opinion in Organ Transplantation · doi:10.1097/mot.0000000000000532 · Cited by 36
Hassanein, W., Uluer, M. C., Langford, J. T., Woodall, J. D., Cimeno, A., Dhru, U., Werdesheim, A., Harrison, J., Rivera-Pratt, C., Klepfer, S., et al. (2016) · Organogenesis · doi:10.1080/15476278.2016.1276146 · Cited by 36
Baptista, P. M., Moran, E. C., Vyas, D., Ribeiro, M. H. L., Atala, A., Sparks, J. L., & Söker, S. (2016) · Tissue Engineering Part C Methods · doi:10.1089/ten.tec.2015.0334 · Cited by 36
Moser, P. T., & Ott, H. C. (2014) · Current Opinion in Organ Transplantation · doi:10.1097/mot.0000000000000131 · Cited by 36
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Wu, Q., Li, Y., Wang, Y., Li, L., Jiang, X. Q., Tang, J., Yang, H., Zhang, J., Bao, J., & Bu, H. (2016) · Journal of Biomedical Materials Research Part A · doi:10.1002/jbm.a.35843 · Cited by 33
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Sudo, R. (2014) · Organogenesis · doi:10.4161/org.27968 · Cited by 32
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Meng, F., Almohanna, F., Altuhami, A., Assiri, A. M., & Broering, D. (2019) · Journal of Biomedical Materials Research Part A · doi:10.1002/jbm.a.36551 · Cited by 31
Higashi, H., Yagi, H., Kuroda, K., Tajima, K., Kojima, H., Nishi, K., Morisaku, T., Hirukawa, K., Fukuda, K., Matsubara, K., et al. (2022) · American Journal of Transplantation · doi:10.1111/ajt.16928 · Cited by 31
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Uygun, B. E., & Yarmush, M. L. (2013) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2013.05.008 · Cited by 33
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Anderson, B. D., Nelson, E. D., Joo, D., Amiot, B. P., Katane, A. A., Mendenhall, A., Steiner, B. G., Stumbras, A. R., Nelson, V. L., Palumbo, R. N., et al. (2021) · Communications Biology · doi:10.1038/s42003-021-02665-2 · Cited by 29
Self-citationHussein, K. H., Ahmadzada, B., Correa, J. C., Sultan, A., Wilken, S., Amiot, B., & Nyberg, S. L. (2024) · Bioactive Materials · doi:10.1016/j.bioactmat.2024.06.001 · Cited by 28
“Fortunately, the biliary network tree is confirmed to be intact after decellularization by scan electron microscopy, corrosion cast, dye perfusion, and contrast radiography; only reconstruction by bile duct cells is necessary [12,110,170,198].”
BackgroundMethodAbazari, M. F., Soleimanifar, F., Enderami, S. E., Nasiri, N., Nejati, F., Mousavi, S. A., Soleimani, M., Kiani, J., Ghoraeian, P., & Kehtari, M. (2019) · Journal of Cellular Biochemistry · doi:10.1002/jcb.29351 · Cited by 28
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Lahrood, F. N., Saheli, M., Farzaneh, Z., Taheri, P., Dorraj, M., Baharvand, H., Vosough, M., & Piryaei, A. (2019) · Stem Cells and Development · doi:10.1089/scd.2019.0130 · Cited by 25
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Cardinale, V., Lanthier, N., Baptista, P. M., Carpino, G., Carnevale, G., Orlando, G., Angelico, R., Manzia, T. M., Schuppan, D., Pinzani, M., et al. (2023) · Stem Cell Reports · doi:10.1016/j.stemcr.2023.06.005 · Cited by 27
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Lee, E., Kim, H. J., Shaker, M. R., Ryu, J. R., Ham, M. S., Seo, S. H., Kim, D. H., Lee, K., Jung, N., Choe, Y., et al. (2019) · ACS Biomaterials Science & Engineering · doi:10.1021/acsbiomaterials.9b00219 · Cited by 24
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Butter, A., Aliyev, K., Hillebrandt, K. H., Raschzok, N., Kluge, M., Seiffert, N., Tang, P., Napierala, H., Muhamma, A. I., Reutzel‐Selke, A., et al. (2016) · Journal of Tissue Engineering and Regenerative Medicine · doi:10.1002/term.2383 · Cited by 24
On the road to bioartificial organs
Ren, X., & Ott, H. C. (2014) · Pflügers Archiv - European Journal of Physiology · doi:10.1007/s00424-014-1504-4 · Cited by 22
Wadström, J., Ericzon, B., Halloran, P. F., Bechstein, W. O., Opelz, G., Serón, D., Grinyo, J. M., Loupy, A., Kuypers, D. R. J., Mariat, C., et al. (2017) · Transplantation · doi:10.1097/tp.0000000000001563 · Cited by 23
Asadi, M., Khalili, M., Lotfi, H., Moghaddam, S. V., Zarghami, N., André, H., & Alizadeh, E. (2021) · Life Sciences · doi:10.1016/j.lfs.2021.119373 · Cited by 22
Toprakhisar, B., Verfaillie, C. M., & Kumar, M. (2023) · Cells · doi:10.3390/cells12020301 · Cited by 26
Whole liver engineering: A promising approach to develop functional liver surrogates
Meng, F., Assiri, A. M., Dhar, D. K., & Broering, D. (2017) · Liver International · doi:10.1111/liv.13444 · Cited by 23
Two Decades of Advances and Limitations in Organ Recellularization
Stoian, A., Adil, A., Biniazan, F., & Haykal, S. (2024) · Current Issues in Molecular Biology · doi:10.3390/cimb46080543 · Cited by 21
An organ-derived extracellular matrix triggers in situ kidney regeneration in a preclinical model
Tajima, K., Yagi, H., Morisaku, T., Nishi, K., Kushige, H., Kojima, H., Higashi, H., Kuroda, K., Kitago, M., Adachi, S., et al. (2022) · npj Regenerative Medicine · doi:10.1038/s41536-022-00213-y · Cited by 19
Self-citationZheng, X., Xiang, J., Wu, W., Wang, B., Liu, W., Gao, R., Dong, D., & Lv, Y. (2015) · Biomedical Materials · doi:10.1088/1748-6041/10/4/045023 · Cited by 20
Xiang, J., Zheng, X., Liu, P., Yang, L., Dong, D., Wu, W., Liu, X., Li, J., & Lv, Y. (2016) · Organogenesis · doi:10.1080/15476278.2016.1185584 · Cited by 20
Ebrahim, N., Badr, O. A. M., Yousef, M. M., Hassouna, A., Sabry, D., Farid, A., Mostafa, O., AlSaihati, H., Seleem, Y., Aziz, E. A. E., et al. (2021) · Cells · doi:10.3390/cells10112819 · Cited by 21
Bio-scaffolds in organ-regeneration: Clinical potential and current challenges
Yesmin, S., Paget, M. B., Murray, H. E., & Downing, R. (2017) · Current Research in Translational Medicine · doi:10.1016/j.retram.2017.08.002 · Cited by 19
Chu, T. L., Tripathi, G., Bae, S. H., & Lee, B. (2021) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2021.10.128 · Cited by 19
Potential alternative approaches to xenotransplantation
Mou, L., Chen, F., Dai, Y., Cai, Z., & Cooper, D. K. C. (2015) · International Journal of Surgery · doi:10.1016/j.ijsu.2015.06.085 · Cited by 18
Mußbach, F., Settmacher, U., Dirsch, O., Xie, C., & Dahmen, U. (2016) · European Surgical Research · doi:10.1159/000446211 · Cited by 18
Pang, Y., Horimoto, Y., Sutoko, S., Montagne, K., Shinohara, M., Mathiue, D., Komori, K., Anzai, M., Niino, T., & Sakai, Y. (2016) · Biofabrication · doi:10.1088/1758-5090/8/3/035016 · Cited by 17
Decellularization of canine kidney for three-dimensional organ regeneration
Tajima, K., Kuroda, K., Otaka, Y., Kinoshita, R., Kita, M., OYAMADA, T., & Kanai, K. (2020) · Veterinary World · doi:10.14202/vetworld.2020.452-457 · Cited by 17
Singh, G., Senapati, S., Satpathi, S., Behera, P. K., Das, B., & Nayak, B. (2022) · The FASEB Journal · doi:10.1096/fj.202200807r · Cited by 15
Decellularized Tissue Engineering
Shirakigawa, N., & Ijima, H. (2017) · Advanced structured materials · doi:10.1007/978-981-10-3328-5_5 · Cited by 15
Recent advances in optimization of liver decellularization procedures used for liver regeneration
Khajavi, M., Hashemi, M., & Kalalinia, F. (2021) · Life Sciences · doi:10.1016/j.lfs.2021.119801 · Cited by 14
Aravalli, R. N., & Steer, C. J. (2020) · Genes · doi:10.3390/genes11070729 · Cited by 13
Optimizing the Decellularized Porcine Liver Scaffold Protocol
Faccioli, L. A. P., Dias, G. S., Hoff, V., Dias, M. L., Pimentel, C. F., Hochman‐Mendez, C., Parente, D. B., Labrunie, E. M., Mourão, P. A. S., Salvalaggio, P. R. D. O., et al. (2020) · Cells Tissues Organs · doi:10.1159/000510297 · Cited by 14
Metadata
- Authors
- Yagi, H., Fukumitsu, K., Fukuda, K., Kitago, M., Shinoda, M., Obara, H., Itano, O., Kawachi, S., Tanabe, M., Coudriet, G. M., Piganelli, J. D., Gilbert, T. W., Soto-Gutierrez, A., & Kitagawa, Y.
- Affiliations
- Keio University (JP)
- University of Pittsburgh (US)
- Department of Pathology, Center for Innovative Regenerative Therapies, Department of Surgery, Transplantation Section, Children's Hospital of Pittsburgh and the Starzl Transplantation Institute, University of Pittsburgh, Pittsburgh, PA, USA
- Corresponding author
- Kitagawa, Yuko
- ORCID
- Venue
- Cell Transplantation
- Publisher
- SAGE Publications
- Published
- 2013-02
- Volume
- 22
- Issue
- 2
- Pages
- 231-242
- ISSN
- 0963-6897 (ISSN-L)
- 1555-3892
- PMC
- PMC3682787
- OpenAlex
- W2073329698
- Cited by
- 188
- References
- 34
- Citations by year
- 2026: 3 · 2025: 9 · 2024: 5 · 2023: 8 · 2022: 16 · 2021: 14 · 2020: 9 · 2019: 17 · 2018: 15 · 2017: 20 · 2016: 25 · 2015: 24 · 2014: 13 · 2013: 8
- FWCI
- 15.35
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Cell Transplantation (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- Funders
- Astellas Pharma
- Japan Society for the Promotion of Science (23689059)
- National Institute of Diabetes and Digestive and Kidney Diseases (K99DK083556, DK083556, R00DK083556)
- Topics
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Health Sciences › Medicine › Surgery › Organ Transplantation Techniques and Outcomes
- Keywords
- liver transplantation, decellularization, porcine liver, extracellular matrix, perfusion protocols, hepatocyte engraftment, organ culture, liver regeneration, angiogenesis, organ shortage, regenerative medicine
- MeSH terms
- Animals, Cells, Cultured, Liver, Male, Swine, Liver Transplantation, Hepatocytes, Tissue Engineering, Regenerative Medicine
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