Cited by 122Muscle metabolism and nutritionNutrition and Health in AgingNutrition and Health Studies

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Dietary Protein Requirement of Female Adults >65 Years Determined by the Indicator Amino Acid Oxidation Technique Is Higher Than Current Recommendations

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Rafii, Mahroukh, Chapman, Karen, Owens, Jillian, Elango, Rajavel, Campbell, Wayne W, Ball, Ronald O, Pencharz, Paul B, Courtney-Martin, Glenda·2015·The Journal of Nutrition

DOI 10.3945/jn.114.197517

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Abstract

Background Studies on protein requirements in vulnerable groups such as older adults are few, and results are conflicting.Objective The main objective of this study was to determine the protein requirements of free-living women >65 y by measuring the oxidation of l-[1-(13)C]phenylalanine to (13)CO2 in response to graded intakes of protein.Methods Twelve subjects participated in the study, with protein intakes ranging from 0.2 to 2.0 g · kg(-1) · d(-1) for a total of 82 studies. The diets provided energy at 1.5 times each subject's resting energy expenditure and were isocaloric. Protein was given as an amino acid mixture on the basis of the egg protein pattern, except for phenylalanine and tyrosine, which were maintained constant across the protein intake amounts. All subjects were adapted for 2 d before the study day to a protein intake of 1.0 g · kg(-1) · d(-1). The mean protein requirement was determined by applying a mixed-effects change-point regression analysis to F(13)CO2 (label tracer oxidation in (13)CO2 breath), which identified a breakpoint in the F(13)CO2 in response to graded amounts of protein.Results The mean estimated average requirement (EAR) and upper 95% CI (approximating the RDA) protein requirement of women >65 y were 0.96 and 1.29 g · kg(-1) · d(-1), respectively.Conclusion These estimates of protein requirements for older women are higher than the current EAR and RDA based on nitrogen balance data, which are 0.66 and 0.80 g · kg(-1) · d(-1), respectively. This trial was registered at clinicaltrials.gov as NCT01604980.

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Authors: Rafii, M., Chapman, K., Owens, J., Elango, R., Campbell, W. W., Ball, R. O., Pencharz, P. B., & Courtney-Martin, G.
Affiliations: Hospital for Sick Children (CA)
University of British Columbia (CA)
Purdue University West Lafayette (US)
University of Alberta (CA)
ORCID: Elango, Rajavel
Campbell, Wayne W
Venue: The Journal of Nutrition
Publisher: Elsevier BV
Published: 2015-01
Volume: 145
Issue: 1
Pages: 18-24
ISSN: 0022-3166 (ISSN-L)
DOI: 10.3945/jn.114.197517
OpenAlex: W2115817612
Cited by: 122
References: 64
Citations by year: 2026: 4 · 2025: 9 · 2024: 8 · 2023: 11 · 2022: 11 · 2021: 7 · 2020: 11 · 2019: 7 · 2018: 17 · 2017: 12 · 2016: 18 · 2015: 5
FWCI: 5.98
Type: journal article
Language: en
Open access: bronze
doi.org
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
www.elsevier.com/open-access/userlicense/1.0/ (vor, 2949 d)
Locations: Journal of Nutrition (publishedVersion)
PubMed (publishedVersion)
Funders: Pfizer
Mead Johnson Nutrition
Hospital for Sick Children
Canadian Institutes of Health Research (MT10321)
Topics: Health Sciences › Nursing › Nutrition and Dietetics › Muscle metabolism and nutrition
Health Sciences › Medicine › Physiology (medical) › Nutrition and Health in Aging
Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Nutrition and Health Studies
Keywords: protein requirements, older women, indicator amino acid oxidation, phenylalanine oxidation, nitrogen balance, estimated average requirement, RDA
MeSH terms: Age Factors, Aged, Aged, 80 and over, Amino Acids, Energy Intake, Carbon Isotopes, Dietary Proteins, Energy Metabolism, Female, Fluorine, Humans, Isotope Labeling, Nutritional Physiological Phenomena, Nutritional Requirements, Oxidation-Reduction, Phenylalanine, Tyrosine

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Dietary Protein Requirement of Female Adults >65 Years Determined by the Indicator Amino Acid Oxidation Technique Is Higher Than Current Recommendations

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