Chétoui olive leaf extracts: Influence of the solvent type on phenolics and antioxidant activities
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The aim of this study was to investigate the influence of the solvent type on the extraction of phenolics and the antioxidant properties of the extracts obtained from Chétoui olive leaves. Extraction was conducted at room temperature using four solvents: deionized water (ddH2O), 80% methanol (80% MeOH), 70% ethanol (70% EtOH), and 80% acetone. Total phenols and total flavonoids were measured using the Folin-Ciocalteau and aluminum chloride colorimetric methods, respectively. The antioxidant properties have been determined by two scavenging activity methods, DPPH and ABTS. The total phenol and flavonoid contents of the olive leaf extracts (OLEs) ranged from 16.52 to 24.93 mg gallic acid g–1 DM and from 6.23 to 21.47 mg catechin g–1 DM, respectively. The IC50 values of DPPH varied from 0.17 to 0.97 mg mL–1 and the antioxidant activity values determined by ABTS (trolox equivalent antioxidant capacity) were between 629.87 and 1064.25 μmol TE g–1 DM. Our results revealed that extracting solvents have a significant influence on the antioxidant properties of olive leaves and that a methanol mixture is recommended for extracts with high levels of flavonoids and important antioxidant activities. Moreover, it was noticed that the trolox equivalent antioxidant capacity (TEAC) depends more on the flavonoids (r = 0.821) than on the total phenols (r = 0.399).
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A methanol/water mixture was the best solvent to attain olive leaf extracts rich in flavonoids and with considerable antioxidant properties (Abaza et al.).
“These authors also verified that a methanol/water mixture was the best solvent to attain extracts rich in flavonoids and with considerable antioxidant properties.”
From Comparison of Techniques and Solvents on the Antimicrobial and Antioxidant Potential of Extracts from Acacia dealbata and Olea europaea (2020) · cites this paper as [68]
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Anti-Leishmanial Activities of Olea Europaea Subsp. Laperrinei ExtractsPreprint
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- Authors
- Abaza, L., Ben Youssef, N., Manai, H., Mahjoub Haddada, F., Methenni, K., & Zarrouk, M.
- Affiliations
- Center of Biotechnogy of Borj Cédria (TN)
- Venue
- Grasas y Aceites
- Publisher
- Editorial CSIC
- Published
- 2011-03-30
- Volume
- 62
- Issue
- 1
- Pages
- 96-104
- ISSN
- 0017-3495 (ISSN-L)
- 1988-4214
- OpenAlex
- W2062583749
- Cited by
- 97
- References
- 45
- Citations by year
- 2026: 4 · 2025: 2 · 2024: 9 · 2023: 4 · 2022: 7 · 2021: 8 · 2020: 11 · 2019: 10 · 2018: 9 · 2017: 9 · 2016: 9 · 2015: 4 · 2014: 3 · 2013: 2 · 2012: 5
- FWCI
- 1.66
- Type
- journal article
- Language
- en
- Open access
- diamond
- doi.org
- Locations
- Grasas y Aceites (publishedVersion, cc-by)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- Funders
- Ministère de l'Education Nationale, de l'Enseignement Superieur et de la Recherche
- Topics
- Physical Sciences › Chemistry › Organic Chemistry › Edible Oils Quality and Analysis
- Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
- Life Sciences › Agricultural and Biological Sciences › Food Science › Agricultural and Biological Research
- Keywords
- Olea europaea, olive leaf extract, solvent extraction, phenolic compounds, flavonoids, antioxidant activity, DPPH radical scavenging, ABTS radical scavenging, methanol extraction, ethanol extraction, acetone extraction, water extraction
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