Alterations in Marginal Zone Macrophages and Marginal Zone B Cells in Old Mice
Open accessAbstract
Marginal zones (MZs) are architecturally organized for clearance of and rapid response against blood-borne Ags entering the spleen. MZ macrophages (MZMs) and MZ B cells are particularly important in host defense against T-independent pathogens and may be crucial for the prevention of diseases, such as streptococcal pneumonia, that are devastating in older patients. Our objective was to determine whether there are changes in the cellular components of the MZ between old and young mice. Using immunocytochemistry and a blinded scoring system, we observed gross architectural changes in the MZs of old mice, including reduction in the abundance of MZMs surrounding the MZ sinus as well as disruptions in positioning of mucosal addressin cell adhesion molecule 1 (MAdCAM-1)+ sinus lining cells and metallophilic macrophages. Loss of frequency of MZMs was corroborated by flow cytometry. A majority of old mice also showed reduced frequency of MZ B cells, which correlated with decreased abundance of MZM in individual old mice. The spleens of old mice showed less deposition of intravenously injected dextran particles within the MZ, likely because of the decreased frequency in MZMs, because SIGN-R1 expression was not reduced on MZM from old mice. The phagocytic ability of individual MZMs was examined using Staphylococcus aureus bioparticles, and no differences in phagocytosis were found between macrophages from young or old spleens. In summary, an anatomical breakdown of the MZ occurs in advanced age, and a reduction in frequency of MZM may affect the ability of the MZM compartment to clear blood-borne Ags and mount proper T-independent immune responses.
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Marginal zone B cell number is reduced in old mice.
“It was recently reported that marginal zone B cell number is reduced in old mice (60)”
From Causes, consequences, and reversal of immune system aging (2013) · cites this paper as [60]
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- Authors
- Birjandi, S. Z., Ippolito, J. A., Ramadorai, A. K., & Witte, P. L.
- Affiliations
- Loyola University Medical Center (US)
- Corresponding author
- Witte, Pamela L
- ORCID
- Ippolito, Jill A
- Venue
- The Journal of Immunology
- Publisher
- Oxford University Press (OUP)
- Published
- 2011-03
- Volume
- 186
- Issue
- 6
- Pages
- 3441-3451
- ISSN
- 0022-1767 (ISSN-L)
- 1550-6606
- PMC
- PMC3420341
- OpenAlex
- W2121795093
- Cited by
- 92
- References
- 63
- Citations by year
- 2026: 5 · 2025: 5 · 2024: 7 · 2023: 2 · 2022: 7 · 2021: 5 · 2020: 6 · 2019: 6 · 2018: 3 · 2017: 7 · 2016: 5 · 2015: 13 · 2014: 4 · 2013: 10 · 2012: 2
- FWCI
- 2.98
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- The Journal of Immunology (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- University of Chicago
- Loyola University Chicago
- National Institute on Aging (R01AG013874, T32AG031780)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Phagocytosis and Immune Regulation
- Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
- Life Sciences › Immunology and Microbiology › Immunology › Immunotoxicology and immune responses
- Keywords
- marginal zone macrophages, marginal zone B cells, aging, spleen, MAdCAM-1, phagocytosis, streptococcal pneumonia, immunosenescence
- MeSH terms
- Age Factors, Aging, Animals, Antigens, Cell Adhesion, Cell Count, Female, Immunohistochemistry, Lymphopenia, Macrophages, Mice, Inbred BALB C, Spleen, B-Lymphocyte Subsets, Mice
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