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Small Interfering RNA-Mediated Gene Silencing in T Lymphocytes
Open accessAbstract
Introduction of small interfering RNAs (siRNAs) into a cell can cause a specific interference of gene expression known as RNA interference (RNAi). However, RNAi activity in lymphocytes and in normal primary mammalian cells has not been thoroughly demonstrated. In this report, we show that siRNAs complementary to CD4 and CD8α specifically reduce surface expression of these coreceptors and their respective mRNA in a thymoma cell line model. We show that RNAi activity is only caused by a subset of siRNAs complementary to the mRNA target and that ineffective siRNAs can compete with effective siRNAs. Using primary differentiated T lymphocytes, we provide the first evidence of siRNA-mediated RNAi gene silencing in normal nontransformed somatic mammalian lymphocytes.
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“naïve T cells and Th17 cells cannot be transfected effectively in vitro by traditional methods without changing their phenotype or function”
From Dynamic regulatory network controlling TH17 cell differentiation (2013) · cites this paper as [22]
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Rubinson, D. A., Dillon, C. P., Kwiatkowski, A. V., Sievers, C., Yang, L., Kopinja, J. E., Rooney, D. L., Zhang, M., Ihrig, M. M., McManus, M. T., et al. (2003) · Nature Genetics · doi:10.1038/ng1117 · Cited by 1,499
Self-citationKilling the messenger: short RNAs that silence gene expression
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Self-citationIntracellular Delivery by Membrane Disruption: Mechanisms, Strategies, and Concepts
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Dynamic regulatory network controlling TH17 cell differentiation
Yosef, N., Shalek, A. K., Gaublomme, J. T., Jin, H., Lee, Y., Awasthi, A., Wu, C., Karwacz, K., Xiao, S., Jorgolli, M., et al. (2013) · Nature · doi:10.1038/nature11981 · Cited by 682
siRNA Delivery into Human T Cells and Primary Cells with Carbon‐Nanotube Transporters
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Metadata
- Authors
- McManus, M. T., Haines, B. B., Dillon, C. P., Whitehurst, C. E., van Parijs, L., Chen, J., & Sharp, P. A.
- Affiliations
- Massachusetts Institute of Technology (US)
- Center for Cancer Research, Massachusetts Institute of Technology , Cambridge, MA 02139
- ORCID
- Venue
- The Journal of Immunology
- Publisher
- Oxford University Press (OUP)
- Published
- 2002-11
- Volume
- 169
- Issue
- 10
- Pages
- 5754-5760
- ISSN
- 0022-1767 (ISSN-L)
- 1550-6606
- OpenAlex
- W2157078151
- Cited by
- 215
- References
- 40
- Citations by year
- 2025: 1 · 2022: 2 · 2021: 2 · 2020: 4 · 2019: 5 · 2018: 5 · 2017: 2 · 2016: 5 · 2015: 4 · 2014: 6 · 2013: 2 · 2012: 14
- FWCI
- 9.97
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- The Journal of Immunology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Cancer Research Institute
- Arthritis Foundation
- National Institutes of Health (CA42063)
- National Cancer Institute (P01CA042063, P01 CA 42063)
- National Institute of General Medical Sciences (R37-GM34277, R37GM034277)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA regulation and disease
- Keywords
- siRNA, RNA interference, gene silencing, T cells, CD4, CD8A, primary lymphocytes
- MeSH terms
- Animals, Luminescent Proteins, Membrane Glycoproteins, Mice, Inbred C57BL, Mice, Mutant Strains, Mice, Transgenic, RNA, Messenger, Spleen, T-Lymphocytes, Thymus Gland, Tumor Cells, Cultured, CD4 Antigens, CD8 Antigens, Gene Silencing, RNA Stability, RNA, Small Interfering, Green Fluorescent Proteins, Mice
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