Cited by 215RNA Interference and Gene DeliveryRNA Research and SplicingRNA regulation and disease

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Small Interfering RNA-Mediated Gene Silencing in T Lymphocytes

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McManus, Michael T, Haines, Brian B, Dillon, Christopher P, Whitehurst, Charles E, van Parijs, Luk, Chen, Jianzhu, Sharp, Phillip A·2002·The Journal of Immunology

DOI 10.4049/jimmunol.169.10.5754

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Abstract

Introduction of small interfering RNAs (siRNAs) into a cell can cause a specific interference of gene expression known as RNA interference (RNAi). However, RNAi activity in lymphocytes and in normal primary mammalian cells has not been thoroughly demonstrated. In this report, we show that siRNAs complementary to CD4 and CD8α specifically reduce surface expression of these coreceptors and their respective mRNA in a thymoma cell line model. We show that RNAi activity is only caused by a subset of siRNAs complementary to the mRNA target and that ineffective siRNAs can compete with effective siRNAs. Using primary differentiated T lymphocytes, we provide the first evidence of siRNA-mediated RNAi gene silencing in normal nontransformed somatic mammalian lymphocytes.

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Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans
Fire, A., Xu, S., Montgomery, M. K., Kostas, S. A., Driver, S. E., & Mello, C. C. (1998) · Nature · doi:10.1038/35888 · Cited by 15,429
The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14
Lee, R. C., Feinbaum, R. L., & Ambros, V. (1993) · Cell · doi:10.1016/0092-8674(93)90529-y · Cited by 12,948
Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells
Elbashir, S. M., Harborth, J., Lendeckel, W., Yalcin, A., Weber, K., & Tuschl, T. (2001) · Nature · doi:10.1038/35078107 · Cited by 9,456
The 21-nucleotide let-7 RNA regulates developmental timing in Caenorhabditis elegans
Reinhart, B. J., Slack, F. J., Basson, M., Pasquinelli, A. E., Bettinger, J. C., Rougvie, A. E., Horvitz, H. R., & Ruvkun, G. (2000) · Nature · doi:10.1038/35002607 · Cited by 4,989
RNA interference
Hannon, G. J. (2002) · Nature · doi:10.1038/418244a · Cited by 3,651
A Cellular Function for the RNA-Interference Enzyme Dicer in the Maturation of the let-7 Small Temporal RNA
Hutvágner, G., McLachlan, J., Pasquinelli, A. E., Bálint, E., Tuschl, T., & Zamore, P. D. (2001) · Science · doi:10.1126/science.1062961 · Cited by 2,781
Conservation of the sequence and temporal expression of let-7 heterochronic regulatory RNA
Pasquinelli, A. E., Reinhart, B. J., Slack, F. J., Martindale, M. Q., Kuroda, M. I., Maller, B., Hayward, D. C., Ball, E. E., Degnan, B. M., Müller, P., et al. (2000) · Nature · doi:10.1038/35040556 · Cited by 2,671
Genes and Mechanisms Related to RNA Interference Regulate Expression of the Small Temporal RNAs that Control C. elegans Developmental Timing
Grishok, A., Pasquinelli, A. E., Conte, D., Li, N., Parrish, S., Ha, I., Baillie, D. L., Fire, A., Ruvkun, G., & Mello, C. C. (2001) · Cell · doi:10.1016/s0092-8674(01)00431-7 · Cited by 1,908
microRNAs
Ambros, V. (2001) · Cell · doi:10.1016/s0092-8674(01)00616-x · Cited by 1,705
Gene silencing in mammals by small interfering RNAs
McManus, M. T., & Sharp, P. A. (2002) · Nature Reviews Genetics · doi:10.1038/nrg908 · Cited by 1,400
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Research works printed but not linked: 38

1998, Nature, 806
1999, Genes Dev., 3191
1998, Proc. Natl. Acad. Sci. USA, 15502
2001, Cell, 23
2001, Science, 834
2002, Nat. Genet. Rev., 737
2001, RNA, 1509
2002, Nat. Immunol., 597
2002, Nature, 244
2001, Nat. Struct. Biol., 746
2000, Development, 4147
2000, Nat. Cell Biol., 70
2001, Nature, 494
2001, Proc. Natl. Acad. Sci. USA, 9742
2001, Cell, 465
2001, Cell, 297
2001, Science, 1713
2002, J. Biol. Chem., 6667
2001, J. Biol. Chem., 48189
2001, J. Biol. Chem., 12
2001, Science, 173
2002, J. Cell Biol., 87
1995, Proc. Natl. Acad. Sci. USA, 7420
2000, Nature, 901
1993, Cell, 843
1999, Dev. Biol., 671
1989, J. Immunol., 3312
1989, Proc. Natl. Acad. Sci. USA, 998
2000, Mol. Cell, 1077
2002, BioEssays, 119
2002, J. Cell Biol., 17
2002, Annu. Rev. Cell. Dev. Biol., 28
2002, Curr. Biol., R138
2001, Cell, 823
2002, Nat. Med., 3
2000, Nature, 86
2002, Nucleic Acids Res., 1757
2002, Methods, 199

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Riedl, S. A. B., Kaiser, P., Raup, A., Synatschke, C. V., Jérôme, V., & Freitag, R. (2018) · Processes · doi:10.3390/pr6100188 · Cited by 40
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Moreau, L., Barthélémy, P., Li, Y., Luo, D., Prata, C. A. H., & Grinstaff, M. W. (2005) · Molecular BioSystems · doi:10.1039/b503302k · Cited by 39
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Authors: McManus, M. T., Haines, B. B., Dillon, C. P., Whitehurst, C. E., van Parijs, L., Chen, J., & Sharp, P. A.
Affiliations: Massachusetts Institute of Technology (US)
Center for Cancer Research, Massachusetts Institute of Technology , Cambridge, MA 02139
ORCID: McManus, Michael T
Haines, Brian B
Dillon, Christopher P
Whitehurst, Charles E
Chen, Jianzhu
Sharp, Phillip A
Venue: The Journal of Immunology
Publisher: Oxford University Press (OUP)
Published: 2002-11
Volume: 169
Issue: 10
Pages: 5754-5760
ISSN: 0022-1767 (ISSN-L)
1550-6606
DOI: 10.4049/jimmunol.169.10.5754
OpenAlex: W2157078151
Cited by: 215
References: 40
Citations by year: 2025: 1 · 2022: 2 · 2021: 2 · 2020: 4 · 2019: 5 · 2018: 5 · 2017: 2 · 2016: 5 · 2015: 4 · 2014: 6 · 2013: 2 · 2012: 14
FWCI: 9.97
Type: journal article
Language: en
Open access: bronze
doi.org
Licence: academic.oup.com/pages/standard-publication-reuse-rights (vor)
Locations: The Journal of Immunology (publishedVersion)
PubMed (publishedVersion)
Funders: Cancer Research Institute
Arthritis Foundation
National Institutes of Health (CA42063)
National Cancer Institute (P01CA042063, P01 CA 42063)
National Institute of General Medical Sciences (R37-GM34277, R37GM034277)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA regulation and disease
Keywords: siRNA, RNA interference, gene silencing, T cells, CD4, CD8A, primary lymphocytes
MeSH terms: Animals, Luminescent Proteins, Membrane Glycoproteins, Mice, Inbred C57BL, Mice, Mutant Strains, Mice, Transgenic, RNA, Messenger, Spleen, T-Lymphocytes, Thymus Gland, Tumor Cells, Cultured, CD4 Antigens, CD8 Antigens, Gene Silencing, RNA Stability, RNA, Small Interfering, Green Fluorescent Proteins, Mice

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Small Interfering RNA-Mediated Gene Silencing in T Lymphocytes

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