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Small Interfering RNA-Mediated Gene Silencing in T Lymphocytes
Open accessAbstract
Introduction of small interfering RNAs (siRNAs) into a cell can cause a specific interference of gene expression known as RNA interference (RNAi). However, RNAi activity in lymphocytes and in normal primary mammalian cells has not been thoroughly demonstrated. In this report, we show that siRNAs complementary to CD4 and CD8α specifically reduce surface expression of these coreceptors and their respective mRNA in a thymoma cell line model. We show that RNAi activity is only caused by a subset of siRNAs complementary to the mRNA target and that ineffective siRNAs can compete with effective siRNAs. Using primary differentiated T lymphocytes, we provide the first evidence of siRNA-mediated RNAi gene silencing in normal nontransformed somatic mammalian lymphocytes.
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“naïve T cells and Th17 cells cannot be transfected effectively in vitro by traditional methods without changing their phenotype or function”
From Dynamic regulatory network controlling TH17 cell differentiation (2013) · cites this paper as [22]
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Cites
Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans
Fire, A., Xu, S., Montgomery, M. K., Kostas, S. A., Driver, S. E., & Mello, C. C. (1998) · Nature · doi:10.1038/35888 · Cited by 15,429
The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14
Lee, R. C., Feinbaum, R. L., & Ambros, V. (1993) · Cell · doi:10.1016/0092-8674(93)90529-y · Cited by 12,948
Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells
Elbashir, S. M., Harborth, J., Lendeckel, W., Yalcin, A., Weber, K., & Tuschl, T. (2001) · Nature · doi:10.1038/35078107 · Cited by 9,456
The 21-nucleotide let-7 RNA regulates developmental timing in Caenorhabditis elegans
Reinhart, B. J., Slack, F. J., Basson, M., Pasquinelli, A. E., Bettinger, J. C., Rougvie, A. E., Horvitz, H. R., & Ruvkun, G. (2000) · Nature · doi:10.1038/35002607 · Cited by 4,989
Hannon, G. J. (2002) · Nature · doi:10.1038/418244a · Cited by 3,651
Hutvágner, G., McLachlan, J., Pasquinelli, A. E., Bálint, E., Tuschl, T., & Zamore, P. D. (2001) · Science · doi:10.1126/science.1062961 · Cited by 2,781
Conservation of the sequence and temporal expression of let-7 heterochronic regulatory RNA
Pasquinelli, A. E., Reinhart, B. J., Slack, F. J., Martindale, M. Q., Kuroda, M. I., Maller, B., Hayward, D. C., Ball, E. E., Degnan, B. M., Müller, P., et al. (2000) · Nature · doi:10.1038/35040556 · Cited by 2,671
Grishok, A., Pasquinelli, A. E., Conte, D., Li, N., Parrish, S., Ha, I., Baillie, D. L., Fire, A., Ruvkun, G., & Mello, C. C. (2001) · Cell · doi:10.1016/s0092-8674(01)00431-7 · Cited by 1,908
Ambros, V. (2001) · Cell · doi:10.1016/s0092-8674(01)00616-x · Cited by 1,705
Gene silencing in mammals by small interfering RNAs
McManus, M. T., & Sharp, P. A. (2002) · Nature Reviews Genetics · doi:10.1038/nrg908 · Cited by 1,400
Self-citationOn the Role of RNA Amplification in dsRNA-Triggered Gene Silencing
Sijen, T., Fleenor, J., Simmer, F., Thijssen, K. L., Parrish, S., Timmons, L., Plasterk, R. H. A., & Fire, A. Z. (2001) · Cell · doi:10.1016/s0092-8674(01)00576-1 · Cited by 1,287
Olsen, P. H., & Ambros, V. (1999) · Developmental Biology · doi:10.1006/dbio.1999.9523 · Cited by 1,234
Analysis of gene function in somatic mammalian cells using small interfering RNAs
Elbashir, S. M. (2002) · Methods · doi:10.1016/s1046-2023(02)00023-3 · Cited by 1,099
Caplen, N. J., Parrish, S., Imani, F., Fire, A. Z., & Morgan, R. A. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.171251798 · Cited by 1,042
ATR and ATRIP: Partners in Checkpoint Signaling
Cortez, D., Guntuku, S., Qin, J., & Elledge, S. J. (2001) · Science · doi:10.1126/science.1065521 · Cited by 985
Targeted mRNA degradation by double-stranded RNA in vitroPreprint
Tuschl, T., Zamore, P. D., Lehmann, R., Bartel, D. P., & Sharp, P. A. (1999) · Genes & Development · doi:10.1101/gad.13.24.3191 · Cited by 858
Self-citationsiRNA-directed inhibition of HIV-1 infection
Novina, C. D., Murray, M. F., Dykxhoorn, D. M., Beresford, P. J., Riess, J. W., Lee, S., Collman, R. G., Lieberman, J., Shankar, P., & Sharp, P. A. (2002) · Nature Medicine · doi:10.1038/nm725 · Cited by 775
Self-citationPhosphorylation-Dependent Ubiquitination of Cyclin E by the SCF Fbw7 Ubiquitin Ligase
Koepp, D. M., Schaefer, L. K., Ye, X., Keyomarsi, K., Chu, C., Harper, J. W., & Elledge, S. J. (2001) · Science · doi:10.1126/science.1065203 · Cited by 764
Specific interference with gene function by double-stranded RNA in early mouse development
Wianny, F., & Zernicka‐Goetz, M. (1999) · Nature Cell Biology · doi:10.1038/35000016 · Cited by 724
Positional effects of short interfering RNAs targeting the human coagulation trigger Tissue Factor
Holen, T. (2002) · Nucleic Acids Research · doi:10.1093/nar/30.8.1757 · Cited by 706
RNA as a target of double-stranded RNA-mediated genetic interference in Caenorhabditis elegans
Montgomery, M. K., Xu, S., & Fire, A. Z. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.26.15502 · Cited by 593
Selective reduction of dormant maternal mRNAs in mouse oocytes by RNA interference
Svoboda, P., Stein, P., Hayashi, H., & Schultz, R. M. (2000) · Development · doi:10.1242/dev.127.19.4147 · Cited by 411
Functional Anatomy of a dsRNA Trigger
Parrish, S., Fleenor, J., Xu, S., Mello, C. C., & Fire, A. Z. (2000) · Molecular Cell · doi:10.1016/s1097-2765(00)00106-4 · Cited by 403
RNAi as Random Degradative PCR
Lipardi, C., Qin, W., & Paterson, B. M. (2001) · Cell · doi:10.1016/s0092-8674(01)00537-2 · Cited by 373
RNA interference: listening to the sound of silence.
Zamore, P. D. (2001) · Nature Structural Biology · doi:10.1038/nsb0901-746 · Cited by 372
Control of Developmental Timing by MicroRNAs and Their Targets
Pasquinelli, A. E., & Ruvkun, G. (2002) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev.cellbio.18.012502.105832 · Cited by 341
Extracellular Export of Sphingosine Kinase-1 Enzyme
Ancellin, N., Colmont, C. S., Su, J. H., Li, Q., Mittereder, N., Chae, S.-S., Stefansson, S., Liau, G., & Hla, T. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m102841200 · Cited by 280
Bernstein, E., Denli, A. M., & Hannon, G. J. (2001) · PubMed · Cited by 223
Biosynthesis of the Linkage Region of Glycosaminoglycans
Bai, X., Zhou, D., Brown, J. R., Crawford, B. E., Hennet, T., & Esko, J. D. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m107339200 · Cited by 190
Li, L., Mao, J., Sun, L., Liu, W., & Wu, D. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m111131200 · Cited by 168
Banerjee, D., & Slack, F. J. (2002) · BioEssays · doi:10.1002/bies.10046 · Cited by 166
Großhans, H., & Slack, F. J. (2002) · The Journal of Cell Biology · doi:10.1083/jcb.200111033 · Cited by 141
Ohta, T., Essner, R., Ryu, J.-H., Palazzo, R. E., Uetake, Y., & Kuriyama, R. (2002) · The Journal of Cell Biology · doi:10.1083/jcb.200108088 · Cited by 136
RNA interference: antiviral defense and genetic tool
Cullen, B. R. (2002) · Nature Immunology · doi:10.1038/ni0702-597 · Cited by 126
MicroRNAs: Hidden in the Genome
Moss, E. G. (2002) · Current Biology · doi:10.1016/s0960-9822(02)00708-x · Cited by 123
Norment, A. M., Lönberg, N., Lacy, E., & Littman, D. R. (1989) · The Journal of Immunology · doi:10.4049/jimmunol.142.9.3312 · Cited by 52
A secreted form of the human lymphocyte cell surface molecule CD8 arises from alternative splicing.
Giblin, P. A., Ledbetter, J. A., & Kavathas, P. (1989) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.86.3.998 · Cited by 52
Mombaerts, P., Terhorst, C., Jacks, T., Tonegawa, S., & Sancho, J. (1995) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.92.16.7420 · Cited by 50
Wood, H. B. (2002) · Nature reviews. Neuroscience · doi:10.1038/nrn707 · Cited by 17
Flagged first, then by citations
1 of the 40 references couldn’t be matched to a known paper.
Research works printed but not linked: 38
- 1998, Nature, 806
- 1999, Genes Dev., 3191
- 1998, Proc. Natl. Acad. Sci. USA, 15502
- 2001, Cell, 23
- 2001, Science, 834
- 2002, Nat. Genet. Rev., 737
- 2001, RNA, 1509
- 2002, Nat. Immunol., 597
- 2002, Nature, 244
- 2001, Nat. Struct. Biol., 746
- 2000, Development, 4147
- 2000, Nat. Cell Biol., 70
- 2001, Nature, 494
- 2001, Proc. Natl. Acad. Sci. USA, 9742
- 2001, Cell, 465
- 2001, Cell, 297
- 2001, Science, 1713
- 2002, J. Biol. Chem., 6667
- 2001, J. Biol. Chem., 48189
- 2001, J. Biol. Chem., 12
- 2001, Science, 173
- 2002, J. Cell Biol., 87
- 1995, Proc. Natl. Acad. Sci. USA, 7420
- 2000, Nature, 901
- 1993, Cell, 843
- 1999, Dev. Biol., 671
- 1989, J. Immunol., 3312
- 1989, Proc. Natl. Acad. Sci. USA, 998
- 2000, Mol. Cell, 1077
- 2002, BioEssays, 119
- 2002, J. Cell Biol., 17
- 2002, Annu. Rev. Cell. Dev. Biol., 28
- 2002, Curr. Biol., R138
- 2001, Cell, 823
- 2002, Nat. Med., 3
- 2000, Nature, 86
- 2002, Nucleic Acids Res., 1757
- 2002, Methods, 199
Cited by
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Rubinson, D. A., Dillon, C. P., Kwiatkowski, A. V., Sievers, C., Yang, L., Kopinja, J. E., Rooney, D. L., Zhang, M., Ihrig, M. M., McManus, M. T., et al. (2003) · Nature Genetics · doi:10.1038/ng1117 · Cited by 1,499
Self-citationKilling the messenger: short RNAs that silence gene expression
Dykxhoorn, D. M., Novina, C. D., & Sharp, P. A. (2003) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm1129 · Cited by 1,185
Self-citationIntracellular Delivery by Membrane Disruption: Mechanisms, Strategies, and Concepts
Stewart, M. P., Langer, R., & Jensen, K. F. (2018) · Chemical Reviews · doi:10.1021/acs.chemrev.7b00678 · Cited by 767
Dynamic regulatory network controlling TH17 cell differentiation
Yosef, N., Shalek, A. K., Gaublomme, J. T., Jin, H., Lee, Y., Awasthi, A., Wu, C., Karwacz, K., Xiao, S., Jorgolli, M., et al. (2013) · Nature · doi:10.1038/nature11981 · Cited by 682
siRNA Delivery into Human T Cells and Primary Cells with Carbon‐Nanotube Transporters
Liu, Z., Winters, M. A., Holodniy, M., & Dai, H. (2007) · Angewandte Chemie International Edition · doi:10.1002/anie.200604295 · Cited by 666
Metadata
- Authors
- McManus, M. T., Haines, B. B., Dillon, C. P., Whitehurst, C. E., van Parijs, L., Chen, J., & Sharp, P. A.
- Affiliations
- Massachusetts Institute of Technology (US)
- Center for Cancer Research, Massachusetts Institute of Technology , Cambridge, MA 02139
- ORCID
- Venue
- The Journal of Immunology
- Publisher
- Oxford University Press (OUP)
- Published
- 2002-11
- Volume
- 169
- Issue
- 10
- Pages
- 5754-5760
- ISSN
- 0022-1767 (ISSN-L)
- 1550-6606
- OpenAlex
- W2157078151
- Cited by
- 215
- References
- 40
- Citations by year
- 2025: 1 · 2022: 2 · 2021: 2 · 2020: 4 · 2019: 5 · 2018: 5 · 2017: 2 · 2016: 5 · 2015: 4 · 2014: 6 · 2013: 2 · 2012: 14
- FWCI
- 9.97
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- The Journal of Immunology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Cancer Research Institute
- Arthritis Foundation
- National Institutes of Health (CA42063)
- National Cancer Institute (P01CA042063, P01 CA 42063)
- National Institute of General Medical Sciences (R37-GM34277, R37GM034277)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA regulation and disease
- Keywords
- siRNA, RNA interference, gene silencing, T cells, CD4, CD8A, primary lymphocytes
- MeSH terms
- Animals, Luminescent Proteins, Membrane Glycoproteins, Mice, Inbred C57BL, Mice, Mutant Strains, Mice, Transgenic, RNA, Messenger, Spleen, T-Lymphocytes, Thymus Gland, Tumor Cells, Cultured, CD4 Antigens, CD8 Antigens, Gene Silencing, RNA Stability, RNA, Small Interfering, Green Fluorescent Proteins, Mice
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