Cited by 243Alzheimer's disease research and treatmentsAutophagy in Disease and TherapyMesenchymal stem cell research

Mesenchymal stem cells enhance autophagy and increase β-amyloid clearance in Alzheimer disease models

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Shin, Jin Young, Park, Hyun Jung, Kim, Ha Na, Oh, Se Hee, Bae, Jae-Sung, Ha, Hee-Jin, Lee, Phil Hyu·2014·Autophagy

Abstract

Current evidence suggests a central role for autophagy in Alzheimer disease (AD), and dysfunction in the autophagic system may lead to amyloid-β (Aβ) accumulation. Using in vitro and in vivo AD models, the present study investigated whether mesenchymal stem cells (MSCs) could enhance autophagy and thus exert a neuroprotective effect through modulation of Aβ clearance In Aβ-treated neuronal cells, MSCs increased cellular viability and enhanced LC3-II expression compared with cells treated with Aβ only. Immunofluorescence revealed that MSC coculture in Aβ-treated neuronal cells increased the number of LC3-II-positive autophagosomes that were colocalized with a lysosomal marker. Ultrastructural analysis revealed that most autophagic vacuoles (AVs) in Aβ-treated cells were not fused with lysosomes, whereas a large portion of autophagosomes were conjoined with lysosomes in MSCs cocultured with Aβ-treated neuronal cells. Furthermore, MSC coculture markedly increased Aβ immunoreactivity colocalized within lysosomes and decreased intracellular Aβ levels compared with Aβ-treated cells. In Aβ-treated animals, MSC administration significantly increased autophagosome induction, final maturation of late AVs, and fusion with lysosomes. Moreover, MSC administration significantly reduced the level of Aβ in the hippocampus, which was elevated in Aβ-treated mice, concomitant with increased survival of hippocampal neurons. Finally, MSC coculture upregulated BECN1/Beclin 1 expression in AD models. These results suggest that MSCs significantly enhance autolysosome formation and clearance of Aβ in AD models, which may lead to increased neuronal survival against Aβ toxicity. Modulation of the autophagy pathway to repair the damaged AD brain using MSCs would have a significant impact on future strategies for AD treatment.

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Authors
Shin, J. Y., Park, H. J., Kim, H. N., Oh, S. H., Bae, J.-S., Ha, H.-J., & Lee, P. H.
Affiliations
  • Yonsei University (KR)
  • Department of Neurology and Brain Research Institute; Yonsei University College of Medicine; Seoul, Korea
  • Kyungpook National University (KR)
  • Sungkyunkwan University (KR)
Corresponding author
Lee, Phil Hyu
Venue
Autophagy
Published
2013-01-01
Volume
10
Issue
1
Pages
32-44
ISSN
  • 1554-8627 (ISSN-L)
  • 1554-8635
OpenAlex
W2031977308
Cited by
243
References
57
Citations by year
2026: 9 · 2025: 8 · 2024: 15 · 2023: 20 · 2022: 23 · 2021: 27 · 2020: 24 · 2019: 31 · 2018: 21 · 2017: 19 · 2016: 21 · 2015: 13 · 2014: 10 · 2013: 1
FWCI
10.42
Type
journal article
Language
en
Open access
Locations
  • Autophagy (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • YUHSpace (Yonsei University Medical Library) (submittedVersion, cc-by-nc-nd)
  • Figshare (submittedVersion, cc-by)
  • Figshare (cc-by)
Topics
  • Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
  • Health Sciences › Medicine › Epidemiology › Autophagy in Disease and Therapy
  • Health Sciences › Medicine › Genetics › Mesenchymal stem cell research
Keywords
mesenchymal stem cells, Alzheimer's disease, amyloid-beta clearance, autophagosome-lysosome fusion, Beclin-1, LC3-II, neuronal survival, hippocampus, neuroprotection, stem cell therapy
MeSH terms
Beclin-1, Alzheimer Disease, Animals, Autophagy, Cell Line, Cell Survival, Cricetulus, Disease Models, Animal, Cricetinae, Hippocampus, Humans, Lysosomes, Membrane Proteins, Neurons, Phagosomes, Time Factors, Amyloid beta-Peptides, CHO Cells, Neuroprotective Agents, Apoptosis Regulatory Proteins, Mice, Mesenchymal Stem Cells

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