Partially Purified Exopolysaccharide from Lactobacillus plantarum YML009 with Total Phenolic Content, Antioxidant and Free Radical Scavenging Efficacy
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Abstract: This study was aimed to partially purify the exopolysaccharide (EPS) from the culture of Lactobacillus plantarum YML009 using ethanol precipitation method with a yield of 260 mg/L. Analytical evaluationby Bradford and Phenolsulphuric methods revealed the presence of 2.2% and 68.1% total protein and total sugar contents in partially purified EPS, respectively. Further, to confirm the biological potential, the EPS was evaluated for its antioxidant activity in various scavenging models including DPPH and nitrite radicals as well as reducing power ability. The partially purified EPS (5-40 mg/mL) displayed considerable amount of antioxidant efficacy on scavenging DPPH and nitrite radicals by 44.73%, and 43.93%, respectively. Moreover, EPS showed potentreducing power capacity along with total phenolic content 18.96 μg/mg of GAE. Key words: Antioxidant, Exopolysaccharide, Free radical scavenger, Lactobacillus plantarum YML009, Total phenolic content.
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Mechanisms by which bacterial EPS can exert antioxidant activity may include degradation of superoxide anion and hydrogen peroxide through ROS scavenging activity, inhibition of lipid peroxidation, reduction of metal ion chelating activity, and up-regulation of enzymatic and non-enzymatic antioxidant activities.
“the mechanisms by which bacterial EPS can exert antioxidant activity may include degradation of superoxide anion and hydrogen peroxide through ROS scavenging activity, inhibition of lipid peroxidation, reduction of metal ion chelating activity as well as up-regulation of enzymatic and non-enzymatic antioxidant activities [115,116,117].”
From Lactic Acid Bacteria Exopolysaccharides Producers: A Sustainable Tool for Functional Foods (2021) · cites this paper as [116]
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- Authors
- Seo, B.-J., Bajpai, V. K., Rather, I. A., & Park, Y.-H.
- Affiliations
- Yeungnam University (KR)
- Corresponding author
- Park, Yong-Ha
- Venue
- Indian Journal of Pharmaceutical Education and Research
- Publisher
- Manuscript Technomedia LLP
- Published
- 2015-06-23
- Volume
- 49
- Issue
- 4
- Pages
- 282-292
- ISSN
- 0019-5464 (ISSN-L)
- OpenAlex
- W1476008860
- Cited by
- 62
- References
- 34
- Citations by year
- 2026: 2 · 2025: 2 · 2024: 11 · 2023: 9 · 2022: 8 · 2021: 6 · 2020: 8 · 2019: 6 · 2018: 5 · 2017: 5
- FWCI
- 4.45
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- cc-by-nc-nd
- Locations
- Indian Journal of Pharmaceutical Education and Research (publishedVersion, cc-by-nc-nd)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
- Keywords
- exopolysaccharides, Lactobacillus plantarum, antioxidant activity, free radical scavenging, total phenolic content, reducing power, DPPH radical, nitrite radical, ethanol precipitation
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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