Cited by 62Polysaccharides Composition and ApplicationsProbiotics and Fermented FoodsPhytochemicals and Antioxidant Activities

Partially Purified Exopolysaccharide from Lactobacillus plantarum YML009 with Total Phenolic Content, Antioxidant and Free Radical Scavenging Efficacy

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Seo, Byoung-Joo, Bajpai, Vivek Kumar, Rather, Irfan Ahmad, Park, Yong-Ha·2015·Indian Journal of Pharmaceutical Education and Research

Abstract

Abstract: This study was aimed to partially purify the exopolysaccharide (EPS) from the culture of Lactobacillus plantarum YML009 using ethanol precipitation method with a yield of 260 mg/L. Analytical evaluationby Bradford and Phenolsulphuric methods revealed the presence of 2.2% and 68.1% total protein and total sugar contents in partially purified EPS, respectively. Further, to confirm the biological potential, the EPS was evaluated for its antioxidant activity in various scavenging models including DPPH and nitrite radicals as well as reducing power ability. The partially purified EPS (5-40 mg/mL) displayed considerable amount of antioxidant efficacy on scavenging DPPH and nitrite radicals by 44.73%, and 43.93%, respectively. Moreover, EPS showed potentreducing power capacity along with total phenolic content 18.96 μg/mg of GAE. Key words: Antioxidant, Exopolysaccharide, Free radical scavenger, Lactobacillus plantarum YML009, Total phenolic content.

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Crossref · 10.5530/ijper.49.4.6

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34 of 34 references verified0 citation errors · 0 not checkable

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  • Mechanisms by which bacterial EPS can exert antioxidant activity may include degradation of superoxide anion and hydrogen peroxide through ROS scavenging activity, inhibition of lipid peroxidation, reduction of metal ion chelating activity, and up-regulation of enzymatic and non-enzymatic antioxidant activities.

    “the mechanisms by which bacterial EPS can exert antioxidant activity may include degradation of superoxide anion and hydrogen peroxide through ROS scavenging activity, inhibition of lipid peroxidation, reduction of metal ion chelating activity as well as up-regulation of enzymatic and non-enzymatic antioxidant activities [115,116,117].”

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Authors
Seo, B.-J., Bajpai, V. K., Rather, I. A., & Park, Y.-H.
Affiliations
Yeungnam University (KR)
Corresponding author
Park, Yong-Ha
Venue
Indian Journal of Pharmaceutical Education and Research
Published
2015-06-23
Volume
49
Issue
4
Pages
282-292
ISSN
0019-5464 (ISSN-L)
OpenAlex
W1476008860
Cited by
62
References
34
Citations by year
2026: 2 · 2025: 2 · 2024: 11 · 2023: 9 · 2022: 8 · 2021: 6 · 2020: 8 · 2019: 6 · 2018: 5 · 2017: 5
FWCI
4.45
Type
journal article
Language
en
Open access
Licence
cc-by-nc-nd
Locations
Indian Journal of Pharmaceutical Education and Research (publishedVersion, cc-by-nc-nd)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
  • Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
Keywords
exopolysaccharides, Lactobacillus plantarum, antioxidant activity, free radical scavenging, total phenolic content, reducing power, DPPH radical, nitrite radical, ethanol precipitation

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