Chemoprevention of rat liver toxicity and carcinogenesis by Spirulina
Open accessAbstract
Spirulina platensis (SP) is a filamentous cyanobacterium microalgae with potent dietary phyto-antioxidant, anti-inflammatory and anti-cancerous properties. The present study aimed to investigate the chemopreventive effect of SP against rat liver toxicity and carcinogenesis induced by dibutyl nitrosamine (DBN) precursors, and further characterized its underlying mechanisms of action in HepG2 cell line. Investigation by light and electron microscopy showed that DBN treatment induced severe liver injury and histopathological abnormalities, which were prevented by SP supplementation. The incidence of liver tumors was significantly reduced from 80 to 20% by SP. Immunohistochemical results indicated that both PCNA and p53 were highly expressed in the liver of DBN-treated rats, but were significantly reduced by SP supplementation. Molecular analysis indicated that SP treatment inhibited cell proliferation, which was accompanied by increased p21 and decreased Rb expression levels at 48hrs post-treatment. In addition, SP increased Bax and decreased Bcl-2 expression, indicating induction of apoptosis by 48hrs. This is the first report of the in vivo chemopreventive effect of SP against DBN-induced rat liver cytotoxicity and carcinogenesis, suggesting its potential use in chemoprevention of cancer.
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Spirulina platensis preparations showed a chemopreventive effect against carcinogenesis induced by dibutyl nitrosamine with a decrease of the incidence of liver tumors from 80% to 20%.
“Spirulina platensis preparations showed the chemopreventive effect against carcinogenesis induced by dibutyl nitrosamine with the decrease of the incidence of liver tumors from 80% to 20%.”
From Anticancer and Cancer Preventive Properties of Marine Polysaccharides: Some Results and Prospects (2013) · cites this paper as [125]
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- Authors
- Ismail, M. F., Ali, D. A., Fernando, A., Abdraboh, M. E., Gaur, R. L., Ibrahim, W. M., Raj, M. H., & Ouhtit, A.
- Affiliations
- Mansoura University (EG)
- Louisiana State University (US)
- Corresponding author
- Ouhtit, Allal
- Venue
- International Journal of Biological Sciences
- Publisher
- Ivyspring International Publisher
- Published
- 2009
- Volume
- 5
- Issue
- 4
- Pages
- 377-387
- ISSN
- 1449-2288 (ISSN-L)
- PMC
- PMC2695150
- OpenAlex
- W2171145157
- Cited by
- 113
- References
- 55
- Citations by year
- 2026: 4 · 2025: 1 · 2024: 5 · 2023: 5 · 2022: 6 · 2021: 10 · 2020: 8 · 2019: 10 · 2018: 8 · 2017: 7 · 2016: 3 · 2015: 12 · 2014: 7 · 2013: 7 · 2012: 3
- FWCI
- 10.07
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- cc-by-nc
- Locations
- International Journal of Biological Sciences (publishedVersion, cc-by-nc)
- PubMed Central (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmacology › Drug-Induced Hepatotoxicity and Protection
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Cancer and biochemical research
- Keywords
- Spirulina platensis, chemoprevention, hepatocarcinogenesis, hepatotoxicity, liver tumors, HepG2 cells, p53, PCNA, p21, Rb, Bax, Bcl-2, apoptosis, cell proliferation, microalgae
- MeSH terms
- Animals, Antioxidants, Body Weight, Cell Cycle, Carcinoma, Hepatocellular, Humans, Immunohistochemistry, Liver, Liver Neoplasms, Male, Nitrosamines, Blotting, Western, Apoptosis, Chemoprevention, Probiotics, Cell Line, Tumor, Microscopy, Electron, Transmission, Cell Proliferation, Rats, Spirulina
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