Measuring the optimal exposure for single particle cryo-EM using a 2.6 Å reconstruction of rotavirus VP6

Optimal determination of particle orientation, absolute hand, and contrast loss in single-particle electron cryomicroscopy

Rosenthal, P. B., & Henderson, R. (2003) · Journal of Molecular Biology · doi:10.1016/j.jmb.2003.07.013 · Cited by 2,416

cryoSPARC: algorithms for rapid unsupervised cryo-EM structure determination

Punjani, A., Rubinstein, J. L., Fleet, D. J., & Brubaker, M. A. (2017) · Nature Methods · doi:10.1038/nmeth.4169 · Cited by 11,626

A neutralizing human antibody binds to the N-terminal domain of the Spike protein of SARS-CoV-2

Chi, X., Yan, R., Zhang, J., Zhang, G., Zhang, Y., Hao, M., Zhang, Z., Fan, P., Dong, Y., Yang, Y., et al. (2020) · Science · doi:10.1126/science.abc6952 · Cited by 1,385

Structural Basis of Substrate Recognition by the Multidrug Resistance Protein MRP1

Johnson, Z. L., & Chen, J. (2017) · Cell · doi:10.1016/j.cell.2017.01.041 · Cited by 375

Structure of the human LAT1–4F2hc heteromeric amino acid transporter complex

Yan, R., Zhao, X., Lei, J., & Zhou, Q. (2019) · Nature · doi:10.1038/s41586-019-1011-z · Cited by 358

Recognition of the amyloid precursor protein by human γ-secretase

Zhou, R., Yang, G., Guo, X., Zhou, Q., Lei, J., & Shi, Y. (2019) · Science · doi:10.1126/science.aaw0930 · Cited by 304

Structure of the human PKD1-PKD2 complex

Su, Q., Hu, F., Ge, X., Lei, J., Yu, S., Wang, T., Zhou, Q., Mei, C., & Shi, Y. (2018) · Science · doi:10.1126/science.aat9819 · Cited by 284

Structural Basis for the RNA-Guided Ribonuclease Activity of CRISPR-Cas13d

Zhang, C., Konermann, S., Brideau, N. J., Lotfy, P., Wu, X., Novick, S. J., Strutzenberg, T., Griffin, P. R., Hsu, P. D., & Lyumkis, D. (2018) · Cell · doi:10.1016/j.cell.2018.09.001 · Cited by 276

Structure of the membrane-assembled retromer coat determined by cryo-electron tomography

Kovtun, O., Leneva, N., Bykov, Y. S., Ariotti, N., Teasdale, R. D., Schaffer, M., Engel, B. D., Owen, D. J., Briggs, J. A. G., & Collins, B. M. (2018) · Nature · doi:10.1038/s41586-018-0526-z · Cited by 247

Structural basis for the recognition of Sonic Hedgehog by human Patched1

Gong, X., Qian, H., Cao, P., Zhao, X., Zhou, Q., Lei, J., & Yan, N. (2018) · Science · doi:10.1126/science.aas8935 · Cited by 216

Structure Basis for Directional R-loop Formation and Substrate Handover Mechanisms in Type I CRISPR-Cas System

Xiao, Y., Luo, M., Hayes, R. P., Kim, J., Ng, S., Ding, F., Liao, M., & Ke, A. (2017) · Cell · doi:10.1016/j.cell.2017.06.012 · Cited by 208

Cryo-EM structures and atomic model of the HIV-1 strand transfer complex intasome

Passos, D. O., Li, M., Yang, R., Rebensburg, S. V., Ghirlando, R., Jeon, Y., Shkriabai, N., Kvaratskhelia, M., Craigie, R., & Lyumkis, D. (2017) · Science · doi:10.1126/science.aah5163 · Cited by 202

Cryo-EM structure of the ATP-sensitive potassium channel illuminates mechanisms of assembly and gating

Martin, G. M., Yoshioka, C., Rex, E. A., Fay, J. F., Xie, Q., Whorton, M. R., Chen, J. Z., & Shyng, S.-L. (2017) · eLife · doi:10.7554/elife.24149 · Cited by 197

Structural basis of γ-secretase inhibition and modulation by small molecule drugs

Yang, G., Zhou, R., Guo, X., Yan, C., Lei, J., & Shi, Y. (2021) · Cell · doi:10.1016/j.cell.2020.11.049 · Cited by 189

Structural Basis of Low-pH-Dependent Lysosomal Cholesterol Egress by NPC1 and NPC2

Qian, H., Wu, X., Du, X., Yao, X., Zhao, X., Lee, J., Yang, H., & Yan, N. (2020) · Cell · doi:10.1016/j.cell.2020.05.020 · Cited by 185

The α-synuclein hereditary mutation E46K unlocks a more stable, pathogenic fibril structure

Boyer, D. R., Li, B., Sun, C., Fan, W., Zhou, K., Hughes, M. P., Sawaya, M. R., Jiang, L., & Eisenberg, D. S. (2020) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1917914117 · Cited by 179

Structures of human Patched and its complex with native palmitoylated sonic hedgehog

Qi, X., Schmiege, P., Coutavas, E., Wang, J., & Li, X. (2018) · Nature · doi:10.1038/s41586-018-0308-7 · Cited by 178

Structure of a CLC chloride ion channel by cryo-electron microscopy

Park, E., Campbell, E. B., & MacKinnon, R. (2017) · Nature · doi:10.1038/nature20812 · Cited by 164

Helicase-Dependent RNA Decay Illuminated by a Cryo-EM Structure of a Human Nuclear RNA Exosome-MTR4 Complex

Weick, E.-M., Puno, M. R., Januszyk, K., Zinder, J. C., DiMattia, M. A., & Lima, C. D. (2018) · Cell · doi:10.1016/j.cell.2018.05.041 · Cited by 153

Structure of the STRA6 receptor for retinol uptake

Chen, Y., Clarke, O. B., Kim, J., Stowe, S., Kim, Y.-K., Assur, Z., Cavalier, M., Godoy-Ruiz, R., von Alpen, D. C., Manzini, C., et al. (2016) · Science · doi:10.1126/science.aad8266 · Cited by 148

Structure and mechanism of human diacylglycerol O-acyltransferase 1

Wang, L., Qian, H., Nian, Y., Han, Y., Ren, Z., Zhang, H., Hu, L., Prasad, B. V. V., Laganowsky, A., Yan, N., et al. (2020) · Nature · doi:10.1038/s41586-020-2280-2 · Cited by 123

Structural basis for catalysis and substrate specificity of human ACAT1

Qian, H., Zhao, X., Yan, R., Yao, X., Gao, S., Sun, X., Du, X., Yang, H., Wong, C. C. L., & Yan, N. (2020) · Nature · doi:10.1038/s41586-020-2290-0 · Cited by 108

Structural basis for strand-transfer inhibitor binding to HIV intasomes

Passos, D. O., Li, M., Jóźwik, I. K., Zhao, X. Z., Santos-Martins, D., Yang, R., Smith, S. J., Jeon, Y., Forli, S., Hughes, S. H., et al. (2020) · Science · doi:10.1126/science.aay8015 · Cited by 106

Visualization of clustered protocadherin neuronal self-recognition complexes

Brasch, J., Goodman, K. M., Noble, A. J., Rapp, M., Mannepalli, S., Bahna, F., Dandey, V. P., Bepler, T., Berger, B., Maniatis, T., et al. (2019) · Nature · doi:10.1038/s41586-019-1089-3 · Cited by 99

Membrane-anchored HDCR nanowires drive hydrogen-powered CO2 fixation

Dietrich, H. M., Righetto, R. D., Kumar, A., Wietrzynski, W., Trischler, R., Schuller, S. K., Wagner, J., Schwarz, F. M., Engel, B. D., Müller, V., et al. (2022) · Nature · doi:10.1038/s41586-022-04971-z · Cited by 99

Cryo-EM reveals a novel octameric integrase structure for betaretroviral intasome function

Ballandras-Colas, A., Brown, M., Cook, N. J., Dewdney, T. G., Demeler, B., Cherepanov, P., Lyumkis, D., & Engelman, A. N. (2016) · Nature · doi:10.1038/nature16955 · Cited by 98

Architecture and mechanism of metazoan retromer:SNX3 tubular coat assembly

Leneva, N., Kovtun, O., Morado, D. R., Briggs, J. A. G., & Owen, D. J. (2021) · Science Advances · doi:10.1126/sciadv.abf8598 · Cited by 90

Structural basis of ion transport and inhibition in ferroportin

Pan, Y., Ren, Z., Gao, S., Shen, J., Wang, L., Xu, Z., Yu, Y., Bachina, P., Zhang, H., Fan, X., et al. (2020) · Nature Communications · doi:10.1038/s41467-020-19458-6 · Cited by 81

Structure of the DASH/Dam1 complex shows its role at the yeast kinetochore-microtubule interface

Jenni, S., & Harrison, S. C. (2018) · Science · doi:10.1126/science.aar6436 · Cited by 72

Molecular-scale visualization of sarcomere contraction within native cardiomyocytes

Burbaum, L., Schneider, J., Scholze, S., Böttcher, R. T., Baumeister, W., Schwille, P., Plitzko, J. M., & Jasnin, M. (2021) · Nature Communications · doi:10.1038/s41467-021-24049-0 · Cited by 67

Autophagy preferentially degrades non-fibrillar polyQ aggregates

(2024) · doi:10.1016/j.molcel.2024.04.018 · Cited by 51

Determinants shaping the nanoscale architecture of the mouse rod outer segment

Pöge, M., Mahamid, J., Imanishi, S. S., Plitzko, J. M., Palczewski, K., & Baumeister, W. (2021) · eLife · doi:10.7554/elife.72817 · Cited by 50

In situ cryo-electron tomography reveals local cellular machineries for axon branch development

Nedozralova, H., Basnet, N., Ibiricu, I., Bodakuntla, S., Biertümpfel, C., & Mizuno, N. (2022) · Journal of Cell Biology · doi:10.1083/jcb.202106086 · Cited by 43

Integrative modeling reveals the molecular architecture of the intraflagellar transport A (IFT-A) complex

McCafferty, C. L., Papoulas, O., Jordan, M. A., Hoogerbrugge, G., Nichols, C., Pigino, G., Taylor, D. W., Wallingford, J. B., & Marcotte, E. M. (2022) · eLife · doi:10.7554/elife.81977 · Cited by 40

Visualising the cytoskeletal machinery in neuronal growth cones using cryo-electron tomography

Atherton, J., Stouffer, M., Francis, F., & Moores, C. A. (2022) · Journal of Cell Science · doi:10.1242/jcs.259234 · Cited by 34

Cryo-EM structure of the HIV-1 Pol polyprotein provides insights into virion maturation

Harrison, J. J. E. K., Passos, D. O., Bruhn, J. F., Bauman, J. D., Tuberty, L., DeStefano, J. J., Ruiz, F. X., Lyumkis, D., & Arnold, E. (2022) · Science Advances · doi:10.1126/sciadv.abn9874 · Cited by 31

Structural basis for the assembly of the DNA polymerase holoenzyme from a monkeypox virus variant

Li, Y., Shen, Y., Hu, Z., & Yan, R. (2023) · Science Advances · doi:10.1126/sciadv.adg2331 · Cited by 31

Extracellular domain of PepT1 interacts with TM1 to facilitate substrate transport

Shen, J., Hu, M., Fan, X., Ren, Z., Portioli, C., Yan, X., Rong, M., & Zhou, M. (2022) · Structure · doi:10.1016/j.str.2022.04.011 · Cited by 29

Structure of the yeast Swi/Snf complex in a nucleosome free state

Wang, C., Guo, Z., Zhan, X., Yang, F., Wu, M., & Zhang, X. (2020) · Nature Communications · doi:10.1038/s41467-020-17229-x · Cited by 25

CryoDRGN-AI: neural ab initio reconstruction of challenging cryo-EM and cryo-ET datasets

Levy, A., Raghu, R., Feathers, J. R., Grzadkowski, M., Poitevin, F., Johnston, J. D., Vallese, F., Clarke, O. B., Wetzstein, G., & Zhong, E. D. (2025) · Nature Methods · doi:10.1038/s41592-025-02720-4 · Cited by 25

Structure of the mature Rous sarcoma virus lattice reveals a role for IP6 in the formation of the capsid hexamer

Obr, M., Ricana, C. L., Nikulin, N., Feathers, J.-P. R., Klanschnig, M., Thader, A., Johnson, M. C., Vogt, V. M., Schur, F. K. M., & Dick, R. A. (2021) · Nature Communications · doi:10.1038/s41467-021-23506-0 · Cited by 23

Rational exploration of fold atlas for human solute carrier proteins

Xie, T., Chi, X., Huang, B., Ye, F., Zhou, Q., & Huang, J. (2022) · Structure · doi:10.1016/j.str.2022.05.015 · Cited by 23

Structural and functional insights of the human peroxisomal ABC transporter ALDP

Jia, Y., Zhang, Y., Wang, W., Lei, J., Ying, Z., & Yang, G. (2022) · eLife · doi:10.7554/elife.75039 · Cited by 15

Cryo-EM structure of the human Asc-1 transporter complex

Li, Y., Guo, Y., Bröer, A., Dai, L., Brӧer, S., & Yan, R. (2024) · Nature Communications · doi:10.1038/s41467-024-47468-1 · Cited by 12

Doublet microtubule inner junction protein FAP20 recruits tubulin to the microtubule lattice

(2023) · doi:10.1016/j.str.2023.09.010 · Cited by 9

Autophagy preferentially degrades non-fibrillar polyQ aggregates

Zhao, D. Y., Bäuerlein, F. J. B., Saha, I., Hartl, F. U., Baumeister, W., & Wilfling, F. (2023) · doi:10.1101/2023.08.08.552291 · Cited by 3

Rational Exploration of Fold Atlas for Human Solute Carrier Proteins

Xie, T., Chi, X., Huang, B., Ye, F., Zhou, Q., & Huang, J. (2021) · doi:10.1101/2021.08.21.457230