Cited by 59Circadian rhythm and melatoninReproductive biology and impacts on aquatic speciesMarine Invertebrate Physiology and Ecology

Combined transcriptome and proteome profiling reveals specific molecular brain signatures for sex, maturation and circalunar clock phase

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Schenk, Sven, Bannister, Stephanie C, Sedlazeck, Fritz J, Anrather, Dorothea, Minh, Bui Quang, Bileck, Andrea, Hartl, Markus, von Haeseler, Arndt +3 more·2019·eLife

Abstract

Many marine animals, ranging from corals to fishes, synchronise reproduction to lunar cycles. In the annelid Platynereis dumerilii, this timing is orchestrated by an endogenous monthly (circalunar) clock entrained by moonlight. Whereas daily (circadian) clocks cause extensive transcriptomic and proteomic changes, the quality and quantity of regulations by circalunar clocks have remained largely elusive. By establishing a combined transcriptomic and proteomic profiling approach, we provide first systematic insight into the molecular changes in Platynereis heads between circalunar phases, and across sexual differentiation and maturation. Whereas maturation elicits large transcriptomic and proteomic changes, the circalunar clock exhibits only minor transcriptomic, but strong proteomic regulation. Our study provides a versatile extraction technique and comprehensive resources. It corroborates that circadian and circalunar clock effects are likely distinct and identifies key molecular brain signatures for reproduction, sex and circalunar clock phase. Examples include prepro-whitnin/proctolin and ependymin-related proteins as circalunar clock targets.

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  1. Meisel J 1990 Zur Hormonabhängigkeit der Spermatogenese bei Platynereis dumerilii: Licht- und elektronenmikroskopische Befunde sowie experimentelle Untersuchungen in vivo und in vitro. PhD-Thesis Bochum Ruhr-Universität
  2. Zantke J Oberlerchner H Tessmar-Raible K 2015 Keeping clockscoordinated: Crosstalk between light, circadian and circalunar clocks Hideharu N Helm B Annual, Lunar and Tidal Clocks: Patterns and Mechanisms of Nature’sEnigmatic Rhythms Japan Springer

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Authors
Schenk, S., Bannister, S. C., Sedlazeck, F. J., Anrather, D., Minh, B. Q., Bileck, A., Hartl, M., von Haeseler, A., Gerner, C., Raible, F., & Tessmar-Raible, K.
Affiliations
  • University of Vienna (AT)
  • Center of Integrative Bioinformatics Vienna, Max F Perutz Laboratories, University of Vienna, Medical University of Vienna, Vienna BioCenter, Vienna, Austria
  • Research Platform 'Rhythms of Life', University of Vienna, Vienna BioCenter, Vienna, Austria
Corresponding author
  • Schenk, Sven
  • Bannister, Stephanie C
  • Raible, Florian
Venue
eLife
Published
2019-02-15
Volume
8
ISSN
2050-084X (ISSN-L)
OpenAlex
W2911985263
Cited by
59
References
111
Citations by year
2026: 5 · 2025: 4 · 2024: 9 · 2023: 6 · 2022: 10 · 2021: 12 · 2020: 6 · 2019: 7
FWCI
4.83
Type
journal article
Language
en
Open access
Locations
  • eLife (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • European Research Council ((FP7/2007-2013)/ERC Grant Agreement 260304, (FP7/2007-2013)/ERC Grant Agreement 337011)
  • University of Vienna (Research Platform Rhythms of Life)
  • Austrian Science Fund (#AY0041321, #P28970, #I2972, #M1478)
  • Vienna International PostDoctoral Program for Molecular Life Sciences (#GM100402)
  • European Molecular Biology Organization (YIP #2893)
Topics
  • Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Circadian rhythm and melatonin
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Physiology › Reproductive biology and impacts on aquatic species
  • Physical Sciences › Earth and Planetary Sciences › Paleontology › Marine Invertebrate Physiology and Ecology
Keywords
circadian clock, Platynereis dumerilii, sexual differentiation, sexual maturation, gene expression profiling, proteomic profiling, ependymin
MeSH terms
Animals, Annelida, Brain, Female, Male, Phylogeny, Sexual Maturation, Signal Transduction, Proteome, Proteomics, Circadian Clocks, Transcriptome

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