Ex vivo Dynamics of Human Glioblastoma Cells in a Microvasculature‐on‐a‐Chip System Correlates with Tumor Heterogeneity and Subtypes
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Abstract
The perivascular niche (PVN) plays an essential role in brain tumor stem‐like cell (BTSC) fate control, tumor invasion, and therapeutic resistance. Here, a microvasculature‐on‐a‐chip system as a PVN model is used to evaluate the ex vivo dynamics of BTSCs from ten glioblastoma patients. BTSCs are found to preferentially localize in the perivascular zone, where they exhibit either the lowest motility, as in quiescent cells, or the highest motility, as in the invasive phenotype, with migration over long distance. These results indicate that PVN is a niche for BTSCs, while the microvascular tracks may serve as a path for tumor cell migration. The degree of colocalization between tumor cells and microvessels varies significantly across patients. To validate these results, single‐cell transcriptome sequencing (10 patients and 21 750 single cells in total) is performed to identify tumor cell subtypes. The colocalization coefficient is found to positively correlate with proneural (stem‐like) or mesenchymal (invasive) but not classical (proliferative) tumor cells. Furthermore, a gene signature profile including PDGFRA correlates strongly with the “homing” of tumor cells to the PVN. These findings demonstrate that the model can recapitulate in vivo tumor cell dynamics and heterogeneity, representing a new route to study patient‐specific tumor cell functions.
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The perivascular niche (PVN) is a niche for brain tumor stem-like cells (BTSCs).
“It was found that the PVN is a niche for BTSCs, and the microvascular diameter can be used as a pathway for tumor metastasis.”
From Tumor-on-a-chip: from bioinspired design to biomedical application (2021) · cites this paper as [196]
The microvascular diameter can be used as a pathway for tumor metastasis.
“It was found that the PVN is a niche for BTSCs, and the microvascular diameter can be used as a pathway for tumor metastasis.”
From Tumor-on-a-chip: from bioinspired design to biomedical application (2021) · cites this paper as [196]
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Applications of single-cell sequencing in cancer research: progress and perspectives
Lei, Y., Tang, R., Xu, J., Wang, W., Zhang, B., Liu, J., Yu, X., & Shi, S. (2021) · Journal of Hematology & Oncology · doi:10.1186/s13045-021-01105-2 · Cited by 597 · see citing works
Self-citationOrgan-on-a-Chip: A New Paradigm for Drug Development
Ma, C., Peng, Y., Li, H., & Chen, W. (2020) · Trends in Pharmacological Sciences · doi:10.1016/j.tips.2020.11.009 · Cited by 589 · see citing works
Self-citationLi, S., Su, W., Wu, H., Yuan, T., Yuan, C., Liu, J., Deng, G., Gao, X., Chen, Z., Bao, Y., et al. (2020) · Nature Biomedical Engineering · doi:10.1038/s41551-020-0540-y · Cited by 425 · see citing works
Self-citationTumor-on-a-chip: from bioinspired design to biomedical application
Liu, X., Fang, J., Huang, S., Wu, X., Xie, X., Wang, J., Liu, F., Zhang, M., Peng, Z., & Hu, N. (2021) · Microsystems & Nanoengineering · doi:10.1038/s41378-021-00277-8 · Cited by 246 · see citing works
Cui, X., Ma, C., Vasudevaraja, V., Serrano, J., Tong, J., Peng, Y., DeLorenzo, M., Shen, G., Frenster, J. D., Morales, R. T., et al. (2020) · eLife · doi:10.7554/elife.52253 · Cited by 163 · see citing works
Dissecting and rebuilding the glioblastoma microenvironment with engineered materials
Wolf, K. J., Chen, J., Coombes, J. D., Aghi, M. K., & Kumar, S. (2019) · Nature Reviews Materials · doi:10.1038/s41578-019-0135-y · Cited by 159 · see citing works
Endothelial Regulation of Drug Transport in a 3D Vascularized Tumor Model
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Organ‐On‐A‐Chip Models of the Blood–Brain Barrier: Recent Advances and Future Prospects
Kawakita, S., Mandal, K., Mou, L., Mecwan, M. M., Zhu, Y., Li, S., Sharma, S., Hernández, A. L., Nguyen, H. T., Maity, S., et al. (2022) · Small · doi:10.1002/smll.202201401 · Cited by 101 · see citing works
Microfluidic high-throughput 3D cell culture
Ko, J., Park, D., Lee, J., Jung, S., Baek, K., Sung, K. E., Lee, J., & Jeon, N. L. (2024) · Nature Reviews Bioengineering · doi:10.1038/s44222-024-00163-8 · Cited by 88 · see citing works
New Strategy for Promoting Vascularization in Tumor Spheroids in a Microfluidic Assay
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Lim, J., Ching, H., Yoon, J., Jeon, N. L., & Kim, Y. (2021) · Nano Convergence · doi:10.1186/s40580-021-00261-y · Cited by 83 · see citing works
Xiao, Y., Wang, Z., Zhao, M., Deng, Y., Yang, M., Su, G., Yang, K., Qian, C., Hu, X., Liu, Y., et al. (2022) · Frontiers in Immunology · doi:10.3389/fimmu.2022.914236 · Cited by 74 · see citing works
Self-citationRuiz-Garcia, H. J., Alvarado-Estrada, K., Schiapparelli, P. V., Quiñones‐Hinojosa, A., & Trifiletti, D. M. (2020) · Frontiers in Cellular Neuroscience · doi:10.3389/fncel.2020.558381 · Cited by 75 · see citing works
Angiogenic biomaterials to promote therapeutic regeneration and investigate disease progression
Ngo, M. T., & Harley, B. A. C. (2020) · Biomaterials · doi:10.1016/j.biomaterials.2020.120207 · Cited by 70 · see citing works
Winkelman, M. A., Kim, D. Y., Kakarla, S., Grath, A., Silvia, N., & Dai, G. (2021) · Lab on a Chip · doi:10.1039/d1lc00605c · Cited by 68 · see citing works
Self-citationPatient‐derived cancer organoids for drug screening: Basic technology and clinical application
Ren, X., Chen, W., Yang, Q., Li, X., & Xu, L. (2022) · Journal of Gastroenterology and Hepatology · doi:10.1111/jgh.15930 · Cited by 69 · see citing works
Tumor-on-a-chip models combined with mini-tissues or organoids for engineering tumor tissues
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A Dynamic 3D Tumor Spheroid Chip Enables More Accurate Nanomedicine Uptake Evaluation
Zhuang, J., Zhang, J., Wu, M., & Zhang, Y. (2019) · Advanced Science · doi:10.1002/advs.201901462 · Cited by 66 · see citing works
A Robust Method for Perfusable Microvascular Network Formation In Vitro
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Biofabrication Strategies and Engineered In Vitro Systems for Vascular Mechanobiology
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A 3D disease and regeneration model of peripheral nervous system–on–a–chip
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Recent Advances in Microfluidic Platforms for Programming Cell‐Based Living Materials
Zhang, P., Shao, N., & Qin, L. (2021) · Advanced Materials · doi:10.1002/adma.202005944 · Cited by 61 · see citing works
Adjei‐Sowah, E., O’Connor, S. A., Veldhuizen, J., Cascio, C. L., Plaisier, C. L., Mehta, S. V., & Nikkhah, M. (2022) · Advanced Science · doi:10.1002/advs.202201436 · Cited by 59 · see citing works
Glioblastoma-on-a-chip construction and therapeutic applications
Xie, Z., Chen, M., Lian, J., Wang, H., & Ma, J. (2023) · Frontiers in Oncology · doi:10.3389/fonc.2023.1183059 · Cited by 59 · see citing works
Faisal, S. M., Comba, A., Varela, M. L., Argento, A. E., Brumley, E. K., Abel, C. C., Castro, M. G., & Lowenstein, P. R. (2022) · Frontiers in Oncology · doi:10.3389/fonc.2022.1005069 · Cited by 57 · see citing works
Gonzales‐Aloy, E., Ahmed-Cox, A., Tsoli, M., Ziegler, D. S., & Kavallaris, M. (2023) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2023.114777 · Cited by 58 · see citing works
Organ-on-a-chip microengineering for bio-mimicking disease models and revolutionizing drug discovery
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A placenta-on-a-chip model to determine the regulation of FKBPL and galectin-3 in preeclampsia
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Pathophysiology of blood-brain barrier in brain tumor. Novel therapeutic advances using nanomedicine
Sharma, H. S., Muresanu, D. F., Nozari, A., Lafuente, J. V., Tian, Z. R., Sahib, S., Bryukhovetskiy, I., Bryukhovetskiy, A. S., Buzoianu, A. D., Patnaik, R., et al. (2020) · International review of neurobiology · doi:10.1016/bs.irn.2020.03.001 · Cited by 50 · see citing works
Electrophysiology Read-Out Tools for Brain-on-Chip Biotechnology
Forró, C., Caron, D., Angotzi, G. N., Gallo, V., Berdondini, L., Santoro, F., Palazzolo, G., & Panuccio, G. (2021) · Micromachines · doi:10.3390/mi12020124 · Cited by 50 · see citing works
Engineering Organ-on-a-Chip to Accelerate Translational Research
Ko, J., Park, D., Lee, S., Gümüşcü, B., & Jeon, N. L. (2022) · Micromachines · doi:10.3390/mi13081200 · Cited by 52 · see citing works
Slika, H., Karimov, Z., Alimonti, P., Abou‐Mrad, T., Fazio, E. D., Alomari, S., & Tyler, B. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms242216316 · Cited by 49 · see citing works
Gerigk, M., Bulstrode, H. J. C. J., Shi, H., Tönisen, F., Cerutti, C., Morrison, G. M., Rowitch, D. H., & Huang, Y. Y. S. (2021) · Lab on a Chip · doi:10.1039/d1lc00271f · Cited by 48 · see citing works
Cancer Models on Chip: Paving the Way to Large‐Scale Trial Applications
Gil, J. F., Moura, C. S., Silvério, V., Gonçalves, G., & Santos, H. A. (2023) · Advanced Materials · doi:10.1002/adma.202300692 · Cited by 45 · see citing works
Zhang, Y., Zhang, B., Lv, C., Zhang, N., Xing, K., Wang, Z., Lv, R., Yu, M., Xu, C., & Wang, Y. (2023) · Theranostics · doi:10.7150/thno.81407 · Cited by 44 · see citing works
Engineering Breast Cancer On-chip—Moving Toward Subtype Specific Models
Moccia, C., & Haase, K. (2021) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2021.694218 · Cited by 34 · see citing works
Cancer-on-a-chip for precision cancer medicine
Liu, L., Wang, H., Chen, R., Song, Y., Wei, W., Baek, D. S. H., Gillin, M., Kurabayashi, K., & Chen, W. (2025) · Lab on a Chip · doi:10.1039/d4lc01043d · Cited by 47 · see citing works
Ayensa-Jiménez, J., Pérez-Aliacar, M., Ranđelović, T., Oliván, S., Fernández, L. J., Sanz‐Herrera, J. A., Ochoa, I., Doweidar, M. H., & Doblaré, M. (2020) · Scientific Reports · doi:10.1038/s41598-020-78215-3 · Cited by 39 · see citing works
Jiang, G., Chen, H., Huang, J., Song, Q., Chen, Y., Gu, X. Q., Zhen-huan, J., Huang, Y., Lin, Y., Feng, J., et al. (2020) · Advanced Science · doi:10.1002/advs.201903290 · Cited by 38 · see citing works
Review: 3D cell models for organ-on-a-chip applications
Żuchowska, A., Baranowska, P., Flont, M., Brzózka, Z., & Jastrzębska, E. (2024) · Analytica Chimica Acta · doi:10.1016/j.aca.2024.342413 · Cited by 38 · see citing works
Tissue‐Engineered Microvessels: A Review of Current Engineering Strategies and Applications
Zhao, N., Pessell, A. F., Zhu, N., & Searson, P. C. (2024) · Advanced Healthcare Materials · doi:10.1002/adhm.202303419 · Cited by 37 · see citing works
Pun, S., Prakash, A., Demaree, D., Krummel, D. P., Sciumè, G., Sengupta, S., & Barrile, R. (2024) · Advanced Healthcare Materials · doi:10.1002/adhm.202401876 · Cited by 36 · see citing works
Ustun, M., Dabbagh, S. R., Ilci, I., Onder, T. B., & Taşoğlu, S. (2021) · Micromachines · doi:10.3390/mi12050490 · Cited by 37 · see citing works
ZNF117 regulates glioblastoma stem cell differentiation towards oligodendroglial lineage
Liu, J., Wang, X., Chen, A. T., Gao, X., Himes, B. T., Zhang, H., Chen, Z., Wang, J., Sheu, W. C., Deng, G., et al. (2022) · Nature Communications · doi:10.1038/s41467-022-29884-3 · Cited by 36 · see citing works
Self-citationThenuwara, G., Javed, B., Singh, B., & Tian, F. (2024) · Sensors · doi:10.3390/s24092865 · Cited by 35 · see citing works
Ngo, M. T., Sarkaria, J. N., & Harley, B. A. C. (2022) · Advanced Science · doi:10.1002/advs.202201888 · Cited by 34 · see citing works
Luque‐González, M. A., Reis, R. L., Kundu, S. C., & Caballero, D. (2020) · Advanced Biosystems · doi:10.1002/adbi.202000045 · Cited by 34 · see citing works
TME-targeted approaches of brain metastases and its clinical therapeutic evidence
Khan, I. M., Khan, S. U., Sala, H. S. S., Khan, M. U., Din, M. A. U., Khan, S., Hassan, S. S. U., Khan, N. M., & Liu, Y. (2023) · Frontiers in Immunology · doi:10.3389/fimmu.2023.1131874 · Cited by 31 · see citing works
On-chip modeling of tumor evolution: Advances, challenges and opportunities
Li, C., Holman, J. B., Shi, Z., Qiu, B., & Ding, W. (2023) · Materials Today Bio · doi:10.1016/j.mtbio.2023.100724 · Cited by 32 · see citing works
Precision oncology using ex vivo technology: a step towards individualised cancer care?
Williams, S. T., Wells, G., Conroy, S., Gagg, H., Allen, R. J., Rominiyi, O., Helleday, T., Hullock, K., Pennington, C. E. W., Rantala, J., et al. (2022) · Expert Reviews in Molecular Medicine · doi:10.1017/erm.2022.32 · Cited by 32 · see citing works
Recent Developments in Glioblastoma‐On‐A‐Chip for Advanced Drug Screening Applications
Maity, S., Bhuyan, T., Jewell, C., Kawakita, S., Sharma, S., Nguyen, H. T., Najafabadi, A. H., Ermis, M., Falcone, N., Chen, J., et al. (2024) · Small · doi:10.1002/smll.202405511 · Cited by 31 · see citing works
Liu, F., Peng, B., Li, M., Ma, J., Deng, G., Zhang, S., Sheu, W. C., Zou, P., Wu, H., Liu, J., et al. (2021) · Cell Reports Physical Science · doi:10.1016/j.xcrp.2021.100691 · Cited by 30 · see citing works
Self-citationWang, R., Zhang, C., Li, D., & Yao, Y. (2022) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2022.1057913 · Cited by 30 · see citing works
Glioblastoma mechanobiology at multiple length scales
Kondapaneni, R. V., Gurung, S. K., Nakod, P. S., Goodarzi, K., Yakati, V., Lenart, N. A., & Rao, S. S. (2024) · Biomaterials Advances · doi:10.1016/j.bioadv.2024.213860 · Cited by 26 · see citing works
Ugodnikov, A., Persson, H., & Simmons, C. A. (2024) · Lab on a Chip · doi:10.1039/d3lc01027a · Cited by 29 · see citing works
A Scaffold-Free 3-D Co-Culture Mimics the Major Features of the Reverse Warburg Effect In Vitro
Keller, F., Bruch, R., Schneider, R., Meier-Hubberten, J. C., Hafner, M., & Rudolf, R. (2020) · Cells · doi:10.3390/cells9081900 · Cited by 24 · see citing works
Development of Tumor-Vasculature Interaction on Chip Mimicking Vessel Co-Option of Glioblastoma
Bae, J., Kim, M., Han, S., & Park, S. (2022) · BioChip Journal · doi:10.1007/s13206-022-00090-z · Cited by 25 · see citing works
The Applications and Challenges of the Development of In Vitro Tumor Microenvironment Chips
Johnson, A., Reimer, S., Childres, R., Cupp, G., Kohs, T. C. L., McCarty, O. J. T., & Kang, Y. B. (2022) · Cellular and Molecular Bioengineering · doi:10.1007/s12195-022-00755-7 · Cited by 24 · see citing works
Hill, L. K., Bruns, J., & Zustiak, S. P. (2021) · Acta Biomaterialia · doi:10.1016/j.actbio.2021.05.005 · Cited by 24 · see citing works
Piantino, M., Figarol, A., & Matsusaki, M. (2021) · Frontiers in Toxicology · doi:10.3389/ftox.2021.656254 · Cited by 24 · see citing works
Use and application of organ-on-a-chip platforms in cancer research
Yu, Y., Zhou, T., & Cao, L.-L. (2023) · Journal of Cell Communication and Signaling · doi:10.1007/s12079-023-00790-7 · Cited by 25 · see citing works
Rattray, Z., Deng, G., Zhang, S., Shirali, A. R., May, C., Chen, X., Cuffari, B. J., Liu, J., Zou, P., Rattray, N. J. W., et al. (2021) · JCI Insight · doi:10.1172/jci.insight.145875 · Cited by 22 · see citing works
Self-citationSong, J., Bang, S., Choi, N., & Kim, H. N. (2022) · Biomicrofluidics · doi:10.1063/5.0121476 · Cited by 23 · see citing works
Fei, X., Wu, J., Tian, H., Jiang, D., Chen, H., Yan, K., Wang, Y., Zhao, Y., Chen, H., Xie, X., et al. (2024) · Cancer Biomarkers · doi:10.3233/cbm-230486 · Cited by 20 · see citing works
Bang, S., Tahk, D., Choi, Y. H., Lee, S., Lim, J., Lee, B., Kim, B., Kim, H. N., Hwang, N. S.-Y., & Jeon, N. L. (2021) · Advanced Functional Materials · doi:10.1002/adfm.202105475 · Cited by 19 · see citing works
A Microfluidic Organ-On-A-Chip: Into the Next Decade of Bone Tissue Engineering Applied in Dentistry
Syahruddin, M. H., Anggraeni, R., & Ana, I. D. (2023) · Future Science OA · doi:10.2144/fsoa-2023-0061 · Cited by 19 · see citing works
Microphysiological systems for human aging research
Park, S., Laskow, T. C., Chen, J., Guha, P., Dawn, B., & Kim, D. (2024) · Aging Cell · doi:10.1111/acel.14070 · Cited by 20 · see citing works
Self-citationPhenocopying Glioblastoma: A Review
Oraiopoulou, M., Tzamali, E., Papamatheakis, J. D., & Sakkalis, V. (2021) · IEEE Reviews in Biomedical Engineering · doi:10.1109/rbme.2021.3111744 · Cited by 18 · see citing works
Amemiya, T., Hata, N., Mizoguchi, M., Yokokawa, R., Kawamura, Y., Hatae, R., Sangatsuda, Y., Kuga, D., Fujioka, Y., Takigawa, K., et al. (2021) · Molecular Biology Reports · doi:10.1007/s11033-020-06061-7 · Cited by 18 · see citing works
Advances in the application of colorectal cancer organoids in precision medicine
Zhang, Y., Meng, R., Sha, D., Gao, H., Wang, S., Zhou, J., Wang, X., Li, F., Li, X., & Song, W. (2024) · Frontiers in Oncology · doi:10.3389/fonc.2024.1506606 · Cited by 17 · see citing works
Self-citationAdvanced Microfluidics for Single Cell‐Based Cancer Research
Carneiro, A., Palomer, M. A., Esteves, M., Rodrigues, C., Fernandes, J. M., Oliveira, F. A. D. S. D. S., Teixeira, A., Honrado, C., Diéguez, L., Abalde‐Cela, S., et al. (2025) · Advanced Science · doi:10.1002/advs.202500975 · Cited by 17 · see citing works
Ngo, H. T., Amartumur, S., Tran, V. T. A., Tran, M., Diep, Y. N., Cho, H., & Lee, L. P. (2023) · Biosensors · doi:10.3390/bios13020231 · Cited by 15 · see citing works
Vascular microphysiological systems (MPS): biologically relevant and potent models
Breuil, L., Kitada, A., Yadav, S., Zhou, H., Fujimoto, K., & Yokokawa, R. (2025) · Lab on a Chip · doi:10.1039/d5lc00014a · Cited by 15 · see citing works
Samantasinghar, A., Sunildutt, N., Ahmed, F., Memon, F. H., Kang, C.-U., & Choi, K. H. (2025) · ACS Omega · doi:10.1021/acsomega.4c11227 · Cited by 15 · see citing works
Chuaychob, S., Lyu, R., Tanaka, M., Haginiwa, A., Kitada, A., Nakamura, T., & Yokokawa, R. (2024) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2312472121 · Cited by 13 · see citing works
Multi-scale cellular engineering: From molecules to organ-on-a-chip
Huang, N. F., Chaudhuri, O., Cahan, P., Wang, A., Engler, A. J., Wang, Y., Kumar, S., Khademhosseini, A., & Li, S. (2020) · APL Bioengineering · doi:10.1063/1.5129788 · Cited by 14 · see citing works
Flow‐Induced Vascular Remodeling on‐Chip: Implications for Anti‐VEGF Therapy
Mirzapourshafiyi, F., Huber, E., Karr, L., Tong, J., Bausch, A. R., Simmel, F. C., & Alim, K. (2025) · Advanced Functional Materials · doi:10.1002/adfm.202501416 · Cited by 12 · see citing works
Microphysiological systems to study tumor-stroma interactions in brain cancer
Neves, E. R., Harley, B. A. C., & Pedrón, S. (2021) · Brain Research Bulletin · doi:10.1016/j.brainresbull.2021.06.012 · Cited by 12 · see citing works
Considerations for modelling diffuse high-grade gliomas and developing clinically relevant therapies
Higginbottom, S. L., Tomaskovic‐Crook, E., & Crook, J. M. (2023) · Cancer and Metastasis Reviews · doi:10.1007/s10555-023-10100-7 · Cited by 11 · see citing works
Recapitulating the Cancer‐Immunity Cycle on a Chip
Lee, Y., Min, J., Kim, S., Park, W., Ko, J., & Jeon, N. L. (2024) · Advanced Healthcare Materials · doi:10.1002/adhm.202401927 · Cited by 10 · see citing works
Cross-platform analysis reveals cellular and molecular landscape of glioblastoma invasion
Chen, A. T., Xiao, Y., Tang, X., Baqri, M., Gao, X., Reschke, M., Sheu, W. C., Long, G., Zhou, Y., Deng, G., et al. (2022) · Neuro-Oncology · doi:10.1093/neuonc/noac186 · Cited by 10 · see citing works
Self-citationGlioma nanomedicine: Design, fabrication and theranostic application
Wu, D., Zhao, J., Xu, T., Xiang, H., Zhao, B., Gao, L., & Chen, Y. (2024) · Coordination Chemistry Reviews · doi:10.1016/j.ccr.2024.215696 · Cited by 9 · see citing works
Moghaddam, F. D., Rafiee, M., Setayeshfar, A., Moradi, A., Esmaeili, Y., Bertani, F. R., Neisiany, R. E., You, Z., & Zare, E. N. (2024) · Advanced Materials Technologies · doi:10.1002/admt.202400134 · Cited by 9 · see citing works
Microfabricated Organ-Specific Models of Tumor Microenvironments
Oh, J. M., Park, Y., Lee, J., & Shen, K. (2025) · Annual Review of Biomedical Engineering · doi:10.1146/annurev-bioeng-110222-103522 · Cited by 9 · see citing works
Parihar, P., Sunildutt, N., Rahim, C. S. A., Samantasinghar, A., Lim, J., & Choi, K. H. (2024) · Advanced Therapeutics · doi:10.1002/adtp.202300410 · Cited by 10 · see citing works
Biomaterials meet organ-on-chips – a perspective on tumor modeling
Monteiro, C. F., Deus, I. A., Custódio, C. A., & Mano, J. F. (2024) · International Materials Reviews · doi:10.1177/09506608241303436 · Cited by 8 · see citing works
Liang, M., Lei, F., Liu, Y., Lan, D., Huang, H., Zhang, G., Feng, Q., Cao, X., & Dong, H. (2021) · ACS Applied Bio Materials · doi:10.1021/acsabm.1c00534 · Cited by 8 · see citing works
Advances in computational and translational approaches for malignant glioma
Bhargav, A. G., Domino, J. S., Alvarado, A. M., Tuchek, C. A., Akhavan, D., & Camarata, P. J. (2023) · Frontiers in Physiology · doi:10.3389/fphys.2023.1219291 · Cited by 7 · see citing works
Sheidaei, Z., Akbarzadeh, P., Nguyen, N., & Kashaninejad, N. (2022) · Biomedical Microdevices · doi:10.1007/s10544-022-00615-1 · Cited by 5 · see citing works
Lungu, I. I., & Grumezescu, A. M. (2019) · Biomedical Engineering International · doi:10.33263/biomed11.002008 · Cited by 5 · see citing works
Bridging the Gap: How Organ‐on‐a‐Chip Technology Facilitates the Battle against Glioma
Liu, S., Gong, Z., Zhou, D., Ayala‐Nunez, V., Xu, T., & Wick, P. (2025) · Small Science · doi:10.1002/smsc.202500154 · Cited by 5 · see citing works
MEMS in Twenty-First-Century Healthcare
Noushin, T., Parvez, M. S., Biswas, P., Bhuiyan, M. E. H., Rumon, M. A. A., & Tabassum, S. (2025) · Springer tracts in electrical and electronics engineering · doi:10.1007/978-981-96-4659-3_3 · Cited by 5 · see citing works
Mares, A. G., Feiner‐Gracia, N., Muela, Y., Martínez, G., Delgado, L., Albertazzi, L., & Pujals, S. (2021) · Applied Nano · doi:10.3390/applnano2040021 · Cited by 4 · see citing works
Perspective of using in vitro models to understand immunotherapy-induced cytokine release syndrome
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Understanding Glioblastoma Dynamics Using 3D Organoids and Engineered Extracellular Matrix
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On-chip perivascular niche with patient-derived glioma cellsPreprint
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- Authors
- Xiao, Y., Kim, D., Dura, B., Zhang, K., Yan, R., Li, H., Han, E., Ip, J., Zou, P., Liu, J., Chen, A. T., Vortmeyer, A. O., Zhou, J., & Fan, R.
- Affiliations
- Yale University (US)
- Carnegie Mellon University (US)
- Indiana University Health (US)
- Yale Cancer Center (US)
- Corresponding author
- Fan, Rong
- ORCID
- Venue
- Advanced Science
- Publisher
- Wiley
- Published
- 2019-02-10
- Volume
- 6
- Issue
- 8
- Pages
- 1801531-1801531
- ISSN
- 2198-3844 (ISSN-L)
- PMC
- PMC6468969
- OpenAlex
- W2913629863
- Cited by
- 115
- References
- 83
- Citations by year
- 2026: 9 · 2025: 12 · 2024: 20 · 2023: 15 · 2022: 20 · 2021: 21 · 2020: 15 · 2019: 3
- FWCI
- 8.08
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Advanced Science (publishedVersion, cc-by)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- IUScholarWorks (Indiana University) (submittedVersion, cc-by)
- Publisher
- Funders
- National Science Foundation (CBET‐1351443)
- National Cancer Institute (U54 CA193461, U54 CA209992)
- National Institutes of Health (R01 NS095817)
- Topics
- Health Sciences › Medicine › Genetics › Glioma Diagnosis and Treatment
- Health Sciences › Medicine › Oncology › Cancer Cells and Metastasis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Angiogenesis and VEGF in Cancer
- Keywords
- glioblastoma, perivascular niche, tumor heterogeneity, tumor cell migration, tumor cell subtypes, proneural subtype, mesenchymal subtype, PDGFRA, single-cell transcriptomics
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