Recent advances in CE‐MS coupling: Instrumentation, methodology, and applications
Abstract
This review focuses on the latest development of microseparation electromigration methods in capillaries and microfluidic devices coupled with MS for detection and identification of important analytes. It is a continuation of the review article on the same topic by Kleparnik (Electrophoresis 2015, 36, 159–178). A wide selection of 161 relevant articles covers the literature published from June 2014 till May 2016. New improvements in the instrumentation and methodology of MS interfaced with capillary or microfluidic versions of zone electrophoresis, isotachophoresis, and isoelectric focusing are described in detail. The most frequently implemented MS ionization methods include electrospray ionization, matrix‐assisted desorption/ionization and inductively coupled plasma ionization. Although the main attention is paid to the development of instrumentation and methodology, representative examples illustrate also applications in the proteomics, glycomics, metabolomics, biomarker research, forensics, pharmacology, food analysis, and single‐cell analysis. The combinations of MS with capillary versions of electrochromatography, and micellar electrokinetic chromatography are not included.
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Recent advances in CE-MS platforms have attracted considerable interest from the proteomics community as an alternative to LC-MS.
“Recent advances in CE-MS platforms have attracted considerable interest from the proteomics community as an alternative to LC-MS9–11.”
From Recent trends of capillary electrophoresis‐mass spectrometry in proteomics research (2019) · cites this paper as [9]
Solvent consumption in CE-MS is very low, making it environmentally advantageous.
“In addition, solvent consumption (aqueous or organic) in CE-MS is very low, making it environmentally advantageous9.”
From Recent trends of capillary electrophoresis‐mass spectrometry in proteomics research (2019) · cites this paper as [9]
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Cites
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Dai, J., Lamp, J., Xia, Q., & Zhang, Y. (2018) · Analytical Chemistry · doi:10.1021/acs.analchem.7b04608 · Cited by 123 · see citing works
Recent trends of capillary electrophoresis‐mass spectrometry in proteomics research
Gomes, F. P., & Yates, J. R. (2019) · Mass Spectrometry Reviews · doi:10.1002/mas.21599 · Cited by 112 · see citing works
Capillary zone electrophoresis-mass spectrometry for bottom-up proteomics
Zhang, Z., Qu, Y., & Dovichi, N. J. (2018) · TrAC Trends in Analytical Chemistry · doi:10.1016/j.trac.2018.08.008 · Cited by 61 · see citing works
- Authors
- Týčová, A., Ledvina, V., & Klepárník, K.
- Affiliations
- Czech Academy of Sciences (CZ)
- Institute of Analytical Chemistry Czech Academy of Sciences Brno Czech Republic
- Venue
- ELECTROPHORESIS
- Publisher
- Wiley
- Published
- 2016-10-26
- Volume
- 38
- Issue
- 1
- Pages
- 115-134
- ISSN
- 0173-0835 (ISSN-L)
- 1522-2683
- OpenAlex
- W2542746093
- Cited by
- 99
- References
- 14
- Citations by year
- 2026: 6 · 2025: 5 · 2024: 3 · 2023: 4 · 2022: 7 · 2021: 12 · 2020: 12 · 2019: 14 · 2018: 26 · 2017: 9 · 2016: 1
- FWCI
- 1.19
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Electrophoresis (publishedVersion)
- PubMed (publishedVersion)
- ASEP (submittedVersion, public-domain)
- Funders
- Grantová Agentura České Republiky (14–28254s)
- Akademie Věd České Republiky (68081715)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Capillary Electrophoresis Applications
- Physical Sciences › Chemistry › Spectroscopy › Mass Spectrometry Techniques and Applications
- Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
- Keywords
- CE-MS coupling, capillary electrophoresis, microfluidics, zone electrophoresis, isotachophoresis, isoelectric focusing, electrospray ionization, proteomics, glycomics, metabolomics, biomarkers, forensic analysis, food analysis, single-cell analysis
- MeSH terms
- Cell Line, Chromatography, Food Analysis, Humans, Isoelectric Focusing, Mass Spectrometry, Biomarkers, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Electrophoresis, Capillary, Spectrometry, Mass, Electrospray Ionization, Glycomics, Metabolomics, Lab-On-A-Chip Devices, Single-Cell Analysis
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