Capillary Zone Electrophoresis–Electrospray Ionization-Tandem Mass Spectrometry for Top-Down Characterization of the Mycobacterium marinum Secretome
Open accessAbstract
Capillary zone electrophoresis (CZE) with an electrokinetically pumped sheath-flow nanospray interface was coupled with a high-resolution Q-Exactive mass spectrometer for the analysis of culture filtrates from Mycobacterium marinum. We confidently identified 22 gene products from the wildtype M. marinum secretome in a single CZE–tandem mass spectrometry (MS/MS) run. A total of 58 proteoforms were observed with post-translational modifications including signal peptide removal, N-terminal methionine excision, and acetylation. The conductivities of aqueous acetic acid and formic acid solutions were measured from 0.1% to 100% concentration (v/v). Acetic acid (70%) provided lower conductivity than 0.25% formic acid and was evaluated as low ionic-strength and a CZE–MS compatible sample buffer with good protein solubility.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
CRC Handbook of Chemistry and Physics
(2014) · doi:10.1201/b17118 · Cited by 57,706 · see citing works
CRC Handbook of Chemistry and Physics, 2009−2010, 90th ed.
(2009) · Journal of the American Chemical Society · doi:10.1021/ja906434c · Cited by 1,494 · see citing works
Mass spectrometry in proteomics.
Aebersold, R., & Goodlett, D. (2001) · Chemical Reviews · doi:10.1021/cr990076h · Cited by 1,378 · see citing works
Capillary Zone Electrophoresis
Jorgenson, J. W., & Lukacs, K. D. (1983) · Science · doi:10.1126/science.6623076 · Cited by 1,289 · see citing works
Mass spectrometry for proteomics
Han, X., Aslanian, A., & Yates, J. R. (2008) · Current Opinion in Chemical Biology · doi:10.1016/j.cbpa.2008.07.024 · Cited by 743 · see citing works
Mapping intact protein isoforms in discovery mode using top-down proteomics
Tran, J. C., Zamdborg, L., Ahlf, D. R., Lee, J. E., Catherman, A. D., Durbin, K. R., Tipton, J. D., Vellaichamy, A., Kellie, J. F., Li, M., et al. (2011) · Nature · doi:10.1038/nature10575 · Cited by 665 · see citing works
Top Down versus Bottom Up Protein Characterization by Tandem High-Resolution Mass Spectrometry
Kelleher, N. L., Lin, H., Valaskovic, G. A., Aaserud, D. J., Fridriksson, E. K., & McLafferty, F. W. (1999) · Journal of the American Chemical Society · doi:10.1021/ja973655h · Cited by 606 · see citing works
Mass spectrometry-based proteomics: existing capabilities and future directions
Angel, T. E., Aryal, U. K., Hengel, S. M., Baker, E. S., Kelly, R., Robinson, E. W., & Smith, R. D. (2012) · Chemical Society Reviews · doi:10.1039/c2cs15331a · Cited by 443 · see citing works
Mass Spectrometry: Bottom-Up or Top-Down?
Chait, B. T. (2006) · Science · doi:10.1126/science.1133987 · Cited by 442 · see citing works
Attomole Protein Characterization by Capillary Electrophoresis-Mass Spectrometry
Valaskovic, G. A., Kelleher, N. L., & McLafferty, F. W. (1996) · Science · doi:10.1126/science.273.5279.1199 · Cited by 399 · see citing works
Peer Reviewed: Top-Down Proteomics
Kelleher, N. (2004) · Analytical Chemistry · doi:10.1021/ac0415657 · Cited by 386 · see citing works
Ge, Y., Lawhorn, B. G., ElNaggar, M., Strauss, E., Park, J.-H., Begley, T. P., & McLafferty, F. W. (2002) · Journal of the American Chemical Society · doi:10.1021/ja011335z · Cited by 356 · see citing works
Extending Top-Down Mass Spectrometry to Proteins with Masses Greater Than 200 Kilodaltons
Han, X., Jin, M., Breuker, K., & McLafferty, F. W. (2006) · Science · doi:10.1126/science.1128868 · Cited by 328 · see citing works
Proteomics of Integral Membrane ProteinsTheory and Application
Speers, A. E., & Wu, C. C. (2007) · Chemical Reviews · doi:10.1021/cr068286z · Cited by 325 · see citing works
Wojcik, R., Dada, O. O., Sadilek, M., & Dovichi, N. J. (2010) · Rapid Communications in Mass Spectrometry · doi:10.1002/rcm.4672 · Cited by 234 · see citing works
Self-citationJensen, P. K., Paša-Tolić, L., Anderson, G. A., Horner, J. A., Lipton, M., Bruce, J. E., & Smith, R. D. (1999) · Analytical Chemistry · doi:10.1021/ac990196p · Cited by 190 · see citing works
Deconvolution and Database Search of Complex Tandem Mass Spectra of Intact Proteins
Liu, X., Inbar, Y., Dorrestein, P. C., Wynne, C., Edwards, N., Souda, P., Whitelegge, J. P., Bafna, V., & Pevzner, P. A. (2010) · Molecular & Cellular Proteomics · doi:10.1074/mcp.m110.002766 · Cited by 170 · see citing works
McLafferty, F. W., Fridriksson, E. K., Horn, D. M., Lewis, M. A., & Zubarev, R. A. (1999) · Science · doi:10.1126/science.284.5418.1289 · Cited by 166 · see citing works
Catherman, A. D., Durbin, K. R., Ahlf, D. R., Early, B. P., Fellers, R. T., Tran, J. C., Thomas, P. M., & Kelleher, N. L. (2013) · Molecular & Cellular Proteomics · doi:10.1074/mcp.m113.030114 · Cited by 157 · see citing works
Ansong, C., Wu, S., Meng, D., Liu, X., Brewer, H. M., Deatherage Kaiser, B. L., Nakayasu, E. S., Cort, J. R., Pevzner, P., Smith, R. D., et al. (2013) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1221210110 · Cited by 157 · see citing works
Champion, P. A. D., Champion, M. M., Manzanillo, P. S., & Cox, J. S. (2009) · Molecular Microbiology · doi:10.1111/j.1365-2958.2009.06821.x · Cited by 149 · see citing works
Self-citationHaselberg, R., de Jong, G. J., & Somsen, G. W. (2013) · Analytical Chemistry · doi:10.1021/ac303158f · Cited by 149 · see citing works
Li, Y., Champion, M. M., Sun, L., Champion, P. A. D., Wojcik, R., & Dovichi, N. J. (2012) · Analytical Chemistry · doi:10.1021/ac202899p · Cited by 128 · see citing works
Self-citationHaselberg, R., Ratnayake, C. K., de Jong, G. J., & Somsen, G. W. (2010) · Journal of Chromatography A · doi:10.1016/j.chroma.2010.10.006 · Cited by 109 · see citing works
Zhu, G., Sun, L., Yan, X., & Dovichi, N. J. (2013) · Analytical Chemistry · doi:10.1021/ac303750g · Cited by 103 · see citing works
Self-citationOptimizing capillary electrophoresis for top‐down proteomics of 30–80 kDa proteins
Li, Y., Compton, P. D., Tran, J. C., Ntai, I., & Kelleher, N. L. (2014) · PROTEOMICS · doi:10.1002/pmic.201300381 · Cited by 95 · see citing works
Vellaichamy, A., Tran, J. C., Catherman, A. D., Lee, J. E., Kellie, J. F., Sweet, S., Zamdborg, L., Thomas, P. M., Ahlf, D. R., Durbin, K. R., et al. (2010) · Analytical Chemistry · doi:10.1021/ac9021083 · Cited by 94 · see citing works
Chi, A., Bai, D. L., Geer, L. Y., Shabanowitz, J., & Hunt, D. F. (2006) · International Journal of Mass Spectrometry · doi:10.1016/j.ijms.2006.09.030 · Cited by 89 · see citing works
Top Down Proteomics of Human Membrane Proteins from Enriched Mitochondrial Fractions
Catherman, A. D., Li, M., Tran, J. C., Durbin, K. R., Compton, P. D., Early, B. P., Thomas, P. M., & Kelleher, N. L. (2013) · Analytical Chemistry · doi:10.1021/ac3031527 · Cited by 76 · see citing works
Sun, L., Knierman, M. D., Zhu, G., & Dovic̀hi, N. J. (2013) · Analytical Chemistry · doi:10.1021/ac4008122 · Cited by 65 · see citing works
Self-citationLi, Y., Wojcik, R., Dovic̀hi, N. J., & Champion, M. M. (2012) · Analytical Chemistry · doi:10.1021/ac300926h · Cited by 52 · see citing works
Self-citationSimó, C., Herrero, M., Neusüß, C., Pelzing, M., Kenndler, E., Barbas, C., Ibáñez, E., & Cifuentes, A. (2005) · Electrophoresis · doi:10.1002/elps.200500055 · Cited by 45 · see citing works
Tipton, J. D., Tran, J. C., Catherman, A. D., Ahlf, D. R., Durbin, K. R., Lee, J. E., Kellie, J. F., Kelleher, N. L., Hendrickson, C. L., & Marshall, A. G. (2012) · Analytical Chemistry · doi:10.1021/ac202651v · Cited by 40 · see citing works
Yan, X., Essaka, D. C., Sun, L., Zhu, G., & Dovic̀hi, N. J. (2013) · PROTEOMICS · doi:10.1002/pmic.201300062 · Cited by 40 · see citing works
Self-citationSun, L., Zhu, G., Li, Y., Wojcik, R., Yang, P., & Dovic̀hi, N. J. (2012) · PROTEOMICS · doi:10.1002/pmic.201200100 · Cited by 37 · see citing works
Self-citationQiao, Y., Di, Z., Ma, Y., Ma, P., & Xia, S. (2010) · Chinese Journal of Chemical Engineering · doi:10.1016/s1004-9541(10)60242-x · Cited by 28 · see citing works
Flagged first, then by citations
Research works printed but not linked: 3
- Kelleher N. L. Anal. Chem. 2004, 76, 197A– 203A. 15190879
- Kelleher N. L.; Lin H. Y.; Valaskovic G. A.; Aaserud D. J.; Fridriksson E. K.; McLafferty F. W. J. Am. Chem. Soc. 1999, 121, 806– 812.
- Qiao Y.; Di Z.; Ma Y.; Ma P.; Xia S. Chin. J. Chem. Eng. 2010, 18, 446– 454.
Cited by
Sorted by citations
Feist, P. E., & Hummon, A. B. (2015) · International Journal of Molecular Sciences · doi:10.3390/ijms16023537 · Cited by 289 · see citing works
Top-Down Proteomics: Ready for Prime Time?
Chen, B., Brown, K. A., Lin, Z., & Ge, Y. (2018) · Analytical Chemistry · doi:10.1021/acs.analchem.7b04747 · Cited by 210 · see citing works
Harstad, R. K., Johnson, A. C., Weisenberger, M. M., & Bowser, M. T. (2015) · Analytical Chemistry · doi:10.1021/acs.analchem.5b04125 · Cited by 170 · see citing works
Roberts, D. S., Loo, J. A., Tsybin, Y. O., Liu, X., Wu, S., Chamot-Rooke, J., Agar, J. N., Paša-Tolić, L., Smith, L. M., & Ge, Y. (2024) · Nature Reviews Methods Primers · doi:10.1038/s43586-024-00318-2 · Cited by 154 · see citing works
McCool, E. N., Lubeckyj, R. A., Shen, X., Chen, D., Kou, Q., Liu, X., & Sun, L. (2018) · Analytical Chemistry · doi:10.1021/acs.analchem.8b00693 · Cited by 117 · see citing works
Self-citationRecent trends of capillary electrophoresis‐mass spectrometry in proteomics research
Gomes, F. P., & Yates, J. R. (2019) · Mass Spectrometry Reviews · doi:10.1002/mas.21599 · Cited by 112 · see citing works
Recent advances in CE‐MS coupling: Instrumentation, methodology, and applications
Týčová, A., Ledvina, V., & Klepárník, K. (2017) · ELECTROPHORESIS · doi:10.1002/elps.201600366 · Cited by 99 · see citing works
Lubeckyj, R. A., McCool, E. N., Shen, X., Kou, Q., Liu, X., & Sun, L. (2017) · Analytical Chemistry · doi:10.1021/acs.analchem.7b02532 · Cited by 96 · see citing works
Self-citationBush, D. R., Zang, L., Belov, A. M., Ivanov, A. R., & Karger, B. L. (2016) · Analytical Chemistry · doi:10.1021/acs.analchem.5b03218 · Cited by 92 · see citing works
Zhu, G., Sun, L., & Dovic̀hi, N. J. (2015) · Talanta · doi:10.1016/j.talanta.2015.06.003 · Cited by 91 · see citing works
Self-citationCapillary zone electrophoresis-mass spectrometry for top-down proteomics
Shen, X., Yang, Z.-C., McCool, E. N., Lubeckyj, R. A., Chen, D.-Y., & Sun, L.-L. (2019) · Trends in Analytical Chemistry (TrAC) · doi:10.1016/j.trac.2019.115644 · Cited by 71 · see citing works
Self-citationHan, X., Wang, Y., Aslanian, A., Bern, M., Lavallée-Adam, M., & Yates, J. R. (2014) · Analytical Chemistry · doi:10.1021/ac503439n · Cited by 77 · see citing works
Lindenburg, P. W., Haselberg, R., Rozing, G. P., & Ramautar, R. (2014) · Chromatographia · doi:10.1007/s10337-014-2795-5 · Cited by 74 · see citing works
Han, X., Wang, Y., Aslanian, A., Fonslow, B., Graczyk, B., Davis, T. N., & Yates, J. R. (2014) · Journal of Proteome Research · doi:10.1021/pr500971h · Cited by 72 · see citing works
Lubeckyj, R. A., Basharat, A. R., Shen, X., Liu, X., & Sun, L. (2019) · Journal of the American Society for Mass Spectrometry · doi:10.1007/s13361-019-02167-w · Cited by 72 · see citing works
Self-citationŠtěpánová, S., & Kašička, V. (2015) · Journal of Separation Science · doi:10.1002/jssc.201500973 · Cited by 70 · see citing works
Palma‐Cando, A., Woitassek, D., Brunklaus, G., & Scherf, U. (2016) · Materials Chemistry Frontiers · doi:10.1039/c6qm00281a · Cited by 70 · see citing works
Zhao, Y., Sun, L., Zhu, G., & Dovichi, N. J. (2016) · Journal of Proteome Research · doi:10.1021/acs.jproteome.6b00493 · Cited by 61 · see citing works
Self-citationChampion, M. M., Williams, E. A., Pinapati, R. S., & Champion, P. A. D. (2014) · Journal of Proteome Research · doi:10.1021/pr500484w · Cited by 59 · see citing works
Self-citationZhao, Y., Riley, N. M., Sun, L., Hebert, A. S., Yan, X., Westphall, M. S., Rush, M. J. P., Zhu, G., Champion, M. M., Medie, F. M., et al. (2015) · Analytical Chemistry · doi:10.1021/acs.analchem.5b00883 · Cited by 55 · see citing works
Self-citationZhu, G., Sun, L., & Dovichi, N. J. (2016) · The Analyst · doi:10.1039/c6an01140c · Cited by 52 · see citing works
Self-citationDadouch, M., Ladner, Y., & Perrin, C. (2021) · Separations · doi:10.3390/separations8010004 · Cited by 50 · see citing works
Zhao, Y., Sun, L., Knierman, M. D., & Dovichi, N. J. (2016) · Talanta · doi:10.1016/j.talanta.2015.11.020 · Cited by 49 · see citing works
Self-citationDevelopment and Characterizations of a Miniature Capillary Electrophoresis Mass Spectrometry System
He, M., Xue, Z., Zhang, Y., Huang, Z., Fang, X., Qu, F., Ouyang, Z., & Xu, W. L. (2015) · Analytical Chemistry · doi:10.1021/ac504868w · Cited by 46 · see citing works
Dar, A. I., Randhawa, S., Verma, M., Saini, T. C., & Acharya, A. (2025) · Advances in Colloid and Interface Science · doi:10.1016/j.cis.2025.103535 · Cited by 35 · see citing works
Capillary electrophoresis as a powerful tool for the analyses of bacterial samples
Kartsova, L. A., Makeeva, D. V., Kravchenko, A. V., Moskvichev, D. O., & Поликарпова, Д. А. (2020) · TrAC Trends in Analytical Chemistry · doi:10.1016/j.trac.2020.116110 · Cited by 35 · see citing works
Parra, J., Marcoux, J., Poncin, I., Canaan, S., Herrmann, J., Nigou, J., Burlet‐Schiltz, O., & Rivière, M. (2017) · Scientific Reports · doi:10.1038/srep43682 · Cited by 35 · see citing works
Top-Down Mass Spectrometry: Proteomics to Proteoforms
Patrie, S. M. (2016) · Advances in experimental medicine and biology · doi:10.1007/978-3-319-41448-5_8 · Cited by 34 · see citing works
Zhao, Z.-T., Guo, Y., Chowdhury, T., Anjum, S., Li, J.-X., Huang, L.-S., Cupp-Sutton, K. A., Burgett, A. W. G., Shi, D.-J., & Wu, S. (2024) · Analytical Chemistry · doi:10.1021/acs.analchem.4c01119 · Cited by 24 · see citing works
Revealing the potential of capillary electrophoresis/mass spectrometry: the tipping point
Gahoual, R., Leize‐Wagner, E., Houzé, P., & François, Y. (2018) · Rapid Communications in Mass Spectrometry · doi:10.1002/rcm.8238 · Cited by 32 · see citing works
Recent advances of capillary electrophoresis-mass spectrometry instrumentation and methodology
Jiang, Y., He, M.-Y., Zhang, W.-J., Luo, P., Guo, D., Fang, X., & Xu, W. (2017) · Chinese Chemical Letters · doi:10.1016/j.cclet.2017.05.008 · Cited by 30 · see citing works
Thompson, C. R., Champion, M. M., & Champion, P. A. D. (2018) · Journal of Proteome Research · doi:10.1021/acs.jproteome.8b00373 · Cited by 21 · see citing works
Self-citationShen, X., Xu, T., Hakkila, B., Hare, M. G., Wang, Q., Wang, Q., Beckman, J. S., & Sun, L. (2021) · Journal of the American Society for Mass Spectrometry · doi:10.1021/jasms.0c00484 · Cited by 22 · see citing works
Self-citationCapillary Electrophoresis–Mass Spectrometry for Top-Down Proteomics
Wang, Q., Wang, Q., Zhu, G., & Sun, L. (2025) · Annual Review of Analytical Chemistry · doi:10.1146/annurev-anchem-071124-092242 · Cited by 22 · see citing works
Self-citationAnnis, D. S., Ma, H., Balas, D. M., Kumfer, K. T., Sandbo, N. K., Potts, G. K., Coon, J. J., & Mosher, D. F. (2015) · PLoS ONE · doi:10.1371/journal.pone.0135374 · Cited by 21 · see citing works
Top-down protein identification using isotopic envelope fingerprinting
Xiao, K., Yu, F., & Tian, Z. (2016) · Journal of Proteomics · doi:10.1016/j.jprot.2016.10.010 · Cited by 21 · see citing works
Approaches to investigate the role of chelation in the corrosivity of biomass-derived oils
Connatser, R. M., Frith, M. G., Jun, J., Lewis, S. A., Sr., Brady, M. P., & Keiser, J. R. (2019) · Biomass and Bioenergy · doi:10.1016/j.biombioe.2019.105446 · Cited by 20 · see citing works
Redox Proteomics Applied to the Thiol Secretome
Ghezzi, P., & Chan, P. (2016) · Antioxidants and Redox Signaling · doi:10.1089/ars.2016.6732 · Cited by 17 · see citing works
Collars, O. A., Jones, B. S., Hu, D. D., Weaver, S. D., Sherman, T. A., Champion, M. M., & Champion, P. A. D. (2023) · mBio · doi:10.1128/mbio.00987-23 · Cited by 18 · see citing works
Self-citationCapillary Zone Electrophoresis as A Tool for Bottom-Up Protein Analysis
Wojcik, R., Zhu, G., Zhang, Z., Yan, X., Zhao, Y., Sun, L., Champion, M. M., & Dovic̀hi, N. J. (2015) · Bioanalysis · doi:10.4155/bio.15.227 · Cited by 16 · see citing works
Self-citationCE-MS for Proteomics and Intact Protein Analysis
Kuzyk, V., Somsen, G. W., & Haselberg, R. (2021) · Advances in experimental medicine and biology · doi:10.1007/978-3-030-77252-9_4 · Cited by 15 · see citing works
Guo, X., Arceo, J., Huge, B. J., Ludwig, K. R., & Dovic̀hi, N. J. (2016) · The Analyst · doi:10.1039/c5an02116b · Cited by 12 · see citing works
Self-citationDadouch, M., Ladner, Y., Bich, C., Montels, J., Morel, J., Bechara, C., & Perrin, C. (2021) · Journal of Chromatography A · doi:10.1016/j.chroma.2021.462213 · Cited by 11 · see citing works
Kerr, C. M., Schneider, O. L., Tichy, S. E., Huge, B. J., & Champion, M. M. (2025) · Analytical Chemistry · doi:10.1021/acs.analchem.4c05010 · Cited by 10 · see citing works
Self-citationCE-MS for the analysis of intact proteins
Haselberg, R., & Somsen, G. W. (2016) · Wiley-VCH eBooks · doi:10.1002/9783527693801.ch7 · Cited by 10 · see citing works
Flaherty, R. J., Sarver, S. A., Sun, L., Brownell, G. A., Go, D. B., & Dovic̀hi, N. J. (2016) · Journal of the American Society for Mass Spectrometry · doi:10.1007/s13361-016-1529-3 · Cited by 9 · see citing works
Self-citationA method developed to fractionate intact proteins based on capillary electrophoresis
Fu, X., Xiao, H., Liang, S. A., Bao, J. J., Li, T., & Zhang, Y. (2015) · The Analyst · doi:10.1039/c5an01680k · Cited by 7 · see citing works
Capillary Electrophoresis-Mass Spectrometry for Proteomics
Martelli, C., & Desiderio, C. (2018) · Elsevier eBooks · doi:10.1016/b978-0-12-809375-7.00014-9 · Cited by 5 · see citing works
Collars, O. A., Jones, B. S., Hu, D. D., Weaver, S. D., Champion, M. M., & Champion, P. A. D. (2023) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2023.03.14.532585 · Cited by 5 · see citing works
Self-citationTandem Mass Spectrometry in Resolving Complex Gut Microbiota Functions
Kolmeder, C. A., Lähteenmäki, K., Wacklin, P., Kotovuori, A., Ritamo, I., Mättö, J., de Vos, W. M., & Valmu, L. (2017) · doi:10.1002/9781118960226.ch19 · Cited by 2 · see citing works
CE–MS Methods for the Characterization of Monoclonal Antibodies
Reinert, T., Beck, A., Houzé, P., Gahoual, R., & François, Y. (2022) · Wiley eBooks · doi:10.1002/9783527833092.ch11 · Cited by 2 · see citing works
Capillary electrophoresis of glycoproteins
Puerta, A., Gomez-Ruiz, L., Díez‐Masa, J. C., & de Frutos, M. (2021) · Elsevier eBooks · doi:10.1016/b978-0-12-821447-3.00009-3 · Cited by 1 · see citing works
Proteomic Approaches for Diagnostics of Canine and Feline Dementia
Kovačech, B., Zilkova, M., Hanes, J., & Škrabana, R. (2017) · Springer eBooks · doi:10.1007/978-3-319-53219-6_7
Sun, L. (2022) · Capillary Electrophoresis‐Mass Spectrometry for Proteomics and Metabolomics · doi:10.1002/9783527833092.ch10
Self-citation
- Authors
- Zhao, Y., Sun, L., Champion, M. M., Knierman, M. D., & Dovichi, N. J.
- Affiliations
- University of Notre Dame (US)
- Eli Lilly (United States) (US)
- University of Notre Dame , , Notre Dame , ,
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2014-04-11
- Volume
- 86
- Issue
- 10
- Pages
- 4873-4878
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- PMC
- PMC4033641
- OpenAlex
- W2321501735
- Cited by
- 56
- References
- 36
- Citations by year
- 2025: 3 · 2024: 2 · 2023: 2 · 2022: 2 · 2021: 5 · 2020: 2 · 2019: 4 · 2018: 5 · 2017: 6 · 2016: 10 · 2015: 11 · 2014: 4
- FWCI
- 4.42
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Locations
- Analytical Chemistry (publishedVersion, other-oa)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (R01GM096767)
- Topics
- Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
- Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Capillary Electrophoresis Applications
- Physical Sciences › Chemistry › Spectroscopy › Mass Spectrometry Techniques and Applications
- Keywords
- Mycobacterium marinum, secretome, top-down proteomics, capillary zone electrophoresis, proteoforms, post-translational modification, conductivity, culture filtrate, signal peptide removal, N-terminal methionine excision, acetylation
- MeSH terms
- Bacterial Proteins, Peptides, Protein Processing, Post-Translational, Electrophoresis, Capillary, Mycobacterium marinum, Spectrometry, Mass, Electrospray Ionization, Tandem Mass Spectrometry
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review