Sheathless Capillary Electrophoresis-Tandem Mass Spectrometry for Top-Down Characterization of Pyrococcus furiosus Proteins on a Proteome Scale

Han, Xuemei; Wang, Yueju; Aslanian, Aaron; Bern, Marshall; Lavallée-Adam, Mathieu; Yates, John R.

doi:10.1021/ac503439n

Cited by 77Advanced Proteomics Techniques and ApplicationsMass Spectrometry Techniques and ApplicationsMicrofluidic and Capillary Electrophoresis Applications

Sheathless Capillary Electrophoresis-Tandem Mass Spectrometry for Top-Down Characterization of Pyrococcus furiosus Proteins on a Proteome Scale

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Han, Xuemei, Wang, Yueju, Aslanian, Aaron, Bern, Marshall, Lavallée-Adam, Mathieu, Yates, John R.·2014·Analytical Chemistry

DOI 10.1021/ac503439nPMC PMC4238646

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Abstract

Intact protein analysis via top-down mass spectrometry (MS) provides the unique capability of fully characterizing protein isoforms and combinatorial post-translational modifications (PTMs) compared to the bottom-up MS approach. Front-end protein separation poses a challenge for analyzing complex mixtures of intact proteins on a proteomic scale. Here we applied capillary electrophoresis (CE) through a sheathless capillary electrophoresis-electrospray ionization (CESI) interface coupled to an Orbitrap Elite mass spectrometer to profile the proteome from Pyrococcus furiosus. CESI-top-down MS analysis of Pyrococcus furiosus cell lysate identified 134 proteins and 291 proteoforms with a total sample consumption of 270 ng in 120 min of total analysis time. Truncations and various PTMs were detected, including acetylation, disulfide bonds, oxidation, glycosylation, and hypusine. This is the largest scale analysis of intact proteins by CE-top-down MS to date.

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PEI coated capillaries demonstrated high separation efficiency for protein complexes and Pyrococcus furiosus proteins via TDMS.
“Han et al. demonstrated the high separation efficiency of PEI coated capillaries for the analysis of protein complexes and Pyrococcus furiosus proteins via TDMS59,60.”
From Recent trends of capillary electrophoresis‐mass spectrometry in proteomics research (2019) · cites this paper as [60]

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Peptide and protein sequence analysis by electron transfer dissociation mass spectrometry
Syka, J. E. P., Coon, J. J., Schroeder, M. J., Shabanowitz, J., & Hunt, D. F. (2004) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0402700101 · Cited by 2,370 · see citing works
Electron Capture Dissociation of Multiply Charged Protein Cations. A Nonergodic Process
Zubarev, R. A., Kelleher, N. L., & McLafferty, F. W. (1998) · Journal of the American Chemical Society · doi:10.1021/ja973478k · Cited by 1,881 · see citing works
The Orbitrap: a new mass spectrometer
Hu, Q., Noll, R. J., Li, H., Makarov, A., Hardman, M., & Cooks, R. G. (2005) · Journal of Mass Spectrometry · doi:10.1002/jms.856 · Cited by 1,338 · see citing works
Mapping intact protein isoforms in discovery mode using top-down proteomics
Tran, J. C., Zamdborg, L., Ahlf, D. R., Lee, J. E., Catherman, A. D., Durbin, K. R., Tipton, J. D., Vellaichamy, A., Kellie, J. F., Li, M., et al. (2011) · Nature · doi:10.1038/nature10575 · Cited by 665 · see citing works
Top Down versus Bottom Up Protein Characterization by Tandem High-Resolution Mass Spectrometry
Kelleher, N. L., Lin, H., Valaskovic, G. A., Aaserud, D. J., Fridriksson, E. K., & McLafferty, F. W. (1999) · Journal of the American Chemical Society · doi:10.1021/ja973655h · Cited by 606 · see citing works
Improved electrospray ionization interface for capillary zone electrophoresis-mass spectrometry
Smith, R. D., Barinaga, C. J., & Udseth, H. R. (1988) · Analytical Chemistry · doi:10.1021/ac00169a022 · Cited by 534 · see citing works
Mass Spectrometry: Bottom-Up or Top-Down?
Chait, B. T. (2006) · Science · doi:10.1126/science.1133987 · Cited by 442 · see citing works
Peer Reviewed: Top-Down Proteomics
Kelleher, N. (2004) · Analytical Chemistry · doi:10.1021/ac0415657 · Cited by 386 · see citing works
Complete Protein Characterization Using Top-Down Mass Spectrometry and Ultraviolet Photodissociation
Shaw, J. B., Li, W., Holden, D. D., Zhang, Y., Griep-Raming, J., Fellers, R. T., Early, B. P., Thomas, P. M., Kelleher, N. L., & Brodbelt, J. S. (2013) · Journal of the American Chemical Society · doi:10.1021/ja4029654 · Cited by 371 · see citing works
Top Down Characterization of Larger Proteins (45 kDa) by Electron Capture Dissociation Mass Spectrometry
Ge, Y., Lawhorn, B. G., ElNaggar, M., Strauss, E., Park, J.-H., Begley, T. P., & McLafferty, F. W. (2002) · Journal of the American Chemical Society · doi:10.1021/ja011335z · Cited by 356 · see citing works
Extending Top-Down Mass Spectrometry to Proteins with Masses Greater Than 200 Kilodaltons
Han, X., Jin, M., Breuker, K., & McLafferty, F. W. (2006) · Science · doi:10.1126/science.1128868 · Cited by 328 · see citing works
Self-citation
Top Down Proteomics
Yates, J. R., & Kelleher, N. L. (2013) · Analytical Chemistry · doi:10.1021/ac401484r · Cited by 324 · see citing works
Self-citation
Simplifying CE−MS Operation. 2. Interfacing Low-Flow Separation Techniques to Mass Spectrometry Using a Porous Tip
Moini, M. (2007) · Analytical Chemistry · doi:10.1021/ac0704560 · Cited by 322 · see citing works
ProSight PTM 2.0: improved protein identification and characterization for top down mass spectrometry
Zamdborg, L., LeDuc, R. D., Glowacz, K. J., Kim, Y.-B., Viswanathan, V., Spaulding, I. T., Early, B. P., Bluhm, E. J., Babai, S., & Kelleher, N. L. (2007) · Nucleic Acids Research · doi:10.1093/nar/gkm371 · Cited by 251 · see citing works
Simplified capillary electrophoresis nanospray sheath‐flow interface for high efficiency and sensitive peptide analysis
Wojcik, R., Dada, O. O., Sadilek, M., & Dovichi, N. J. (2010) · Rapid Communications in Mass Spectrometry · doi:10.1002/rcm.4672 · Cited by 234 · see citing works
ProSight PTM: an integrated environment for protein identification and characterization by top-down mass spectrometry
LeDuc, R. D., Taylor, G. K., Kim, Y.-B., Januszyk, T. E., Bynum, L., Solà, J., Garavelli, J., & Kelleher, N. (2004) · Nucleic Acids Res. · doi:10.1093/nar/gkh447 · Cited by 192 · see citing works
Probing proteomes using capillary isoelectric focusing-electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry.
Jensen, P. K., Paša-Tolić, L., Anderson, G. A., Horner, J. A., Lipton, M., Bruce, J. E., & Smith, R. D. (1999) · Analytical Chemistry · doi:10.1021/ac990196p · Cited by 190 · see citing works
Processing Complex Mixtures of Intact Proteins for Direct Analysis by Mass Spectrometry
Meng, F., Cargile, B. J., Patrie, S. M., Johnson, J. R., McLoughlin, S. M., & Kelleher, N. L. (2002) · Analytical Chemistry · doi:10.1021/ac020049i · Cited by 183 · see citing works
An integrated analysis of the genome of the hyperthermophilic archaeon Pyrococcus abyssi
Cohen, G. N., Barbe, V., Flament, D., Galperin, M. Y., Heilig, R., Lecompte, O., Poch, O., Prieur, D., Quérellou, J., Ripp, R., et al. (2003) · Molecular Microbiology · doi:10.1046/j.1365-2958.2003.03381.x · Cited by 183 · see citing works
High Capacity Capillary Electrophoresis-Electrospray Ionization Mass Spectrometry: Coupling a Porous Sheathless Interface with Transient-Isotachophoresis
Busnel, J., Schoenmaker, B., Ramautar, R., Carrasco‐Pancorbo, A., Ratnayake, C. K., Feitelson, J. S., Chapman, J. D., Deelder, A. M., & Mayboroda, O. A. (2010) · Analytical Chemistry · doi:10.1021/ac102159d · Cited by 165 · see citing works
Top-down proteomics reveals a unique protein S-thiolation switch in Salmonella Typhimurium in response to infection-like conditions
Ansong, C., Wu, S., Meng, D., Liu, X., Brewer, H. M., Deatherage Kaiser, B. L., Nakayasu, E. S., Cort, J. R., Pevzner, P., Smith, R. D., et al. (2013) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1221210110 · Cited by 157 · see citing works
Protein Identification Using Top-Down Spectra
Liu, X., Sirotkin, Y., Shen, Y., Anderson, G., Tsai, Y. S., Ting, Y. S., Goodlett, D. R., Smith, R. D., Bafna, V., & Pevzner, P. A. (2012) · Molecular & Cellular Proteomics · doi:10.1074/mcp.m111.008524 · Cited by 153 · see citing works
Optimization and Evaluation of a Sheathless Capillary Electrophoresis–Electrospray Ionization Mass Spectrometry Platform for Peptide Analysis: Comparison to Liquid Chromatography–Electrospray Ionization Mass Spectrometry
Faserl, K., Sarg, B., Kremser, L., & Lindner, H. H. (2011) · Analytical Chemistry · doi:10.1021/ac2010372 · Cited by 153 · see citing works
Low-Flow Sheathless Capillary Electrophoresis–Mass Spectrometry for Sensitive Glycoform Profiling of Intact Pharmaceutical Proteins
Haselberg, R., de Jong, G. J., & Somsen, G. W. (2013) · Analytical Chemistry · doi:10.1021/ac303158f · Cited by 149 · see citing works
Improving the Comprehensiveness and Sensitivity of Sheathless Capillary Electrophoresis–Tandem Mass Spectrometry for Proteomic Analysis
Wang, Y., Fonslow, B. R., Wong, C. C. L., Nakorchevsky, A., & Yates, J. R. (2012) · Analytical Chemistry · doi:10.1021/ac301091m · Cited by 144 · see citing works
Self-citation
Enhancing the Coverage of the Urinary Metabolome by Sheathless Capillary Electrophoresis-Mass Spectrometry
Ramautar, R., Busnel, J., Deelder, A. M., & Mayboroda, O. A. (2011) · Analytical Chemistry · doi:10.1021/ac202407v · Cited by 129 · see citing works
A Prokaryotic Gene Cluster Involved in Synthesis of Lysine through the Amino Adipate Pathway: A Key to the Evolution of Amino Acid BiosynthesisPreprint
Nishida, H., Nishiyama, M., Kobashi, N., Kosuge, T., Hoshino, T., & Yamane, H. (1999) · Genome Research · doi:10.1101/gr.9.12.1175 · Cited by 126 · see citing works
Identification of 2D-gel proteins: A comparison of MALDI/TOF peptide mass mapping to μ LC-ESI tandem mass spectrometry
Lim, H., Eng, J. K., Yates, J. R., Tollaksen, S. L., Giometti, C. S., Holden, J. F., Adams, M. W. W., Reich, C. I., Olsen, G. J., & Hays, L. G. (2003) · Journal of the American Society for Mass Spectrometry · doi:10.1016/s1044-0305(03)00144-2 · Cited by 118 · see citing works
Self-citation
Capillary electrophoresis–mass spectrometry for the analysis of intact proteins 2007–2010
Haselberg, R., de Jong, G. J., & Somsen, G. W. (2010) · Electrophoresis · doi:10.1002/elps.201000364 · Cited by 110 · see citing works
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Lee, J. E., Kellie, J. F., Tran, J. C., Tipton, J., Catherman, A. D., Thomas, H. M., Ahlf, D. R., Durbin, K. R., Vellaichamy, A., Ntai, I., et al. (2009) · Journal of the American Society for Mass Spectrometry · doi:10.1016/j.jasms.2009.08.001 · Cited by 104 · see citing works
<scp>CE</scp>‐<scp>MS</scp> for the analysis of intact proteins 2010–2012
Haselberg, R., de Jong, G. J., & Somsen, G. W. (2013) · ELECTROPHORESIS · doi:10.1002/elps.201200439 · Cited by 96 · see citing works
Optimizing capillary electrophoresis for top‐down proteomics of 30–80 kDa proteins
Li, Y., Compton, P. D., Tran, J. C., Ntai, I., & Kelleher, N. L. (2014) · PROTEOMICS · doi:10.1002/pmic.201300381 · Cited by 95 · see citing works
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Vellaichamy, A., Tran, J. C., Catherman, A. D., Lee, J. E., Kellie, J. F., Sweet, S., Zamdborg, L., Thomas, P. M., Ahlf, D. R., Durbin, K. R., et al. (2010) · Analytical Chemistry · doi:10.1021/ac9021083 · Cited by 94 · see citing works
Evaluation of the Compact High-Field Orbitrap for Top-Down Proteomics of Human Cells
Ahlf, D. R., Compton, P. D., Tran, J. C., Early, B. P., Thomas, P. M., & Kelleher, N. L. (2012) · Journal of Proteome Research · doi:10.1021/pr3004216 · Cited by 89 · see citing works
Interpreting Top-Down Mass Spectra Using Spectral Alignment
Frank, A. M., Pesavento, J. J., Mizzen, C. A., Kelleher, N. L., & Pevzner, P. A. (2008) · Analytical Chemistry · doi:10.1021/ac702324u · Cited by 77 · see citing works
Mass Transport in a Micro Flow-Through Vial of a Junction-at-the-Tip Capillary Electrophoresis-Mass Spectrometry Interface
Zhong, X., Maxwell, E. J., & Chen, D. D. Y. (2011) · Analytical Chemistry · doi:10.1021/ac200636y · Cited by 70 · see citing works
Automated Proteomics of E. coli via Top-Down Electron-Transfer Dissociation Mass Spectrometry
Bunger, M. K., Cargile, B. J., Ngunjiri, A., Bundy, J. L., & Stephenson, J. L. (2008) · Analytical Chemistry · doi:10.1021/ac7018409 · Cited by 67 · see citing works
Fast Top-Down Intact Protein Characterization with Capillary Zone Electrophoresis–Electrospray Ionization Tandem Mass Spectrometry
Sun, L., Knierman, M. D., Zhu, G., & Dovic̀hi, N. J. (2013) · Analytical Chemistry · doi:10.1021/ac4008122 · Cited by 65 · see citing works
“Proteotyping”: Population Proteomics of Human Leukocytes Using Top Down Mass Spectrometry
Roth, M. J., Parks, B. A., Ferguson, J. T., Boyne, M. T., & Kelleher, N. L. (2008) · Analytical Chemistry · doi:10.1021/ac800141g · Cited by 65 · see citing works
Capillary electrophoresis–mass spectrometry of intact basic proteins using Polybrene–dextran sulfate–Polybrene-coated capillaries: System optimization and performance
Haselberg, R., de Jong, G. J., & Somsen, G. W. (2010) · Analytica Chimica Acta · doi:10.1016/j.aca.2010.08.032 · Cited by 64 · see citing works
Robust Analysis of the Yeast Proteome under 50 kDa by Molecular-Mass-Based Fractionation and Top-Down Mass Spectrometry
Kellie, J. F., Catherman, A. D., Durbin, K. R., Tran, J. C., Tipton, J., Norris, J. L., Witkowski, C. E., Thomas, P. M., & Kelleher, N. (2011) · Analytical Chemistry · doi:10.1021/ac202384v · Cited by 62 · see citing works
Precursor ion independent algorithm for top-down shotgun proteomics
Tsai, Y. S., Scherl, A., Shaw, J. L., Mackay, C. L., Shaffer, S. A., Langridge‐Smith, P. R. R., & Goodlett, D. R. (2009) · Journal of the American Society for Mass Spectrometry · doi:10.1016/j.jasms.2009.07.024 · Cited by 59 · see citing works
Capillary Zone Electrophoresis–Electrospray Ionization-Tandem Mass Spectrometry for Top-Down Characterization of the Mycobacterium marinum Secretome
Zhao, Y., Sun, L., Champion, M. M., Knierman, M. D., & Dovichi, N. J. (2014) · Analytical Chemistry · doi:10.1021/ac500092q · Cited by 56 · see citing works
Asymmetrical Emitter Geometries for Increased Range of Stable Electrospray Flow Rates
Maxwell, E. J., Zhong, X., & Chen, D. D. Y. (2010) · Analytical Chemistry · doi:10.1021/ac1017953 · Cited by 50 · see citing works
Coupling porous sheathless interface <scp>MS</scp> with transient‐<scp>ITP</scp> in neutral capillaries for improved sensitivity in glycopeptide analysis
Heemskerk, A. A. M., Wuhrer, M., Busnel, J., Koeleman, C. A. M., Selman, M. H. J., Vidarsson, G., Kapur, R., Schoenmaker, B., Derks, R. J. E., Deelder, A. M., et al. (2013) · ELECTROPHORESIS · doi:10.1002/elps.201200357 · Cited by 43 · see citing works
Application of capillary electrophoresis/ electrospray ionization‐mass spectrometry to subcellular proteomics of Escherichia coli ribosomal proteins
Moini, M., & Huang, H.-Y. (2004) · Electrophoresis · doi:10.1002/elps.200305906 · Cited by 42 · see citing works
Mass spectrometry based proteomics, background, status and future needs
Roepstorff, P. (2012) · Protein & Cell · doi:10.1007/s13238-012-2079-5 · Cited by 38 · see citing works
Ultrahigh pressure fast size exclusion chromatography for top‐down proteomics
Chen, X., & Ge, Y. (2013) · PROTEOMICS · doi:10.1002/pmic.201200594 · Cited by 33 · see citing works
Proteomics of Pyrococcus furiosus , a Hyperthermophilic Archaeon Refractory to Traditional Methods
Lee, A. M., Sevinsky, J. R., Bundy, J. L., Grunden, A. M., & Stephenson, J. L. (2009) · Journal of Proteome Research · doi:10.1021/pr801119h · Cited by 20 · see citing works
Proteomics of Pyrococcus furiosus ( Pfu ): Identification of Extracted Proteins by Three Independent Methods
Wong, C. C. L., Cociorva, D., Miller, C. A., Schmidt, A., Monell, C. R., Aebersold, R. H., & Yates, J. R. (2013) · Journal of Proteome Research · doi:10.1021/pr300840j · Cited by 19 · see citing works
Self-citation
Two-dimensional gel electrophoresis mapping of proteins isolated from the hyperthermophile Pyrococcus furiosus
Giometti, C. S., Tollaksen, S. L., Mukund, S., Zhou, Z. H., Ma, K., Mai, X., & Adams, M. W. W. (1995) · Journal of Chromatography A · doi:10.1016/0021-9673(94)01262-d · Cited by 9 · see citing works

Flagged first, then by citations

Research works printed but not linked: 5

Kelleher N. L. Anal. Chem. 2004, 76, 197A– 203A. 15190879
Giometti C. S.; Tollaksen S. L.; Mukund S.; Zhi Hao Z.; Keren M.; Xuhong M.; Adams M. W. W. J. Chromatogr., A 1995, 698, 341– 349.
Kelleher N. L.; Lin H. Y.; Valaskovic G. A.; Aaserud D. J.; Fridriksson E. K.; McLafferty F. W. J. Am. Chem. Soc. 1999, 121, 806– 812.
Smith R. D.; Barinaga C. J.; Udseth H. R. Anal. Chem. 1988, 60, 1948– 1952.
Zubarev R. A.; Kelleher N. L.; McLafferty F. W. J. Am. Chem. Soc. 1998, 120, 3265– 3266.

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Capillary Electrophoresis–Nanoelectrospray Ionization–Selected Reaction Monitoring Mass Spectrometry via a True Sheathless Metal-Coated Emitter Interface for Robust and High-Sensitivity Sample Quantification
Guo, X., Fillmore, T. L., Gao, Y., & Tang, K. (2016) · Analytical Chemistry · doi:10.1021/acs.analchem.5b04912 · Cited by 54 · see citing works
Characterization of cetuximab Fc/2 dimers by off-line CZE-MS
François, Y., Biacchi, M., Saïd, N., Renard, C., Beck, A., Gahoual, R., & Leize‐Wagner, E. (2016) · Analytica Chimica Acta · doi:10.1016/j.aca.2015.12.033 · Cited by 54 · see citing works
A Comprehensive Guide for Performing Sample Preparation and Top-Down Protein Analysis
Padula, M. P., Berry, I. J., O’Rourke, M. B., Raymond, B. B. A., Santos, J., & Djordjevic, S. P. (2017) · Proteomes · doi:10.3390/proteomes5020011 · Cited by 52 · see citing works
Fast separation and analysis of reduced monoclonal antibodies with capillary zone electrophoresis coupled to mass spectrometry
Zhao, Y., Sun, L., Knierman, M. D., & Dovichi, N. J. (2016) · Talanta · doi:10.1016/j.talanta.2015.11.020 · Cited by 49 · see citing works
EThcD and 213 nm UVPD for Top-Down Analysis of Bovine Seminal Plasma Proteoforms on Electrophoretic and Chromatographic Time Frames
Gomes, F. P., Diedrich, J. K., Saviola, A. J., Memili, E., Moura, A. A., & Yates, J. R. (2020) · Analytical Chemistry · doi:10.1021/acs.analchem.9b03856 · Cited by 46 · see citing works
Self-citation
Capillary electrophoresis-mass spectrometry for intact protein analysis: Pharmaceutical and biomedical applications (2018-March 2023).
Maráková, K., Opetová, M., & Tomašovský, R. (2023) · Journal of Separation Science · doi:10.1002/jssc.202300244 · Cited by 39 · see citing works
Automated Capillary Isoelectric Focusing-Tandem Mass Spectrometry for Qualitative and Quantitative Top-Down Proteomics
Xu, T., Shen, X., Yang, Z., Chen, D., Lubeckyj, R. A., McCool, E. N., & Sun, L. (2020) · Analytical Chemistry · doi:10.1021/acs.analchem.0c03266 · Cited by 42 · see citing works
Top-down and middle-down approach by fraction collection enrichment using off-line capillary electrophoresis – mass spectrometry coupling: Application to monoclonal antibody F c/2 charge variants
Biacchi, M., Said, N., Beck, A., Leize-Wagner, E., & François, Y.-N. (2017) · Journal of Chromatography A · doi:10.1016/j.chroma.2017.02.064 · Cited by 41 · see citing works
Enhanced Peptide Detection Toward Single-Neuron Proteomics by Reversed-Phase Fractionation Capillary Electrophoresis Mass Spectrometry
Choi, S. B., Lombard‐Banek, C., Muñoz-LLancao, P., Manzini, M. C., & Nemes, P. (2017) · Journal of the American Society for Mass Spectrometry · doi:10.1007/s13361-017-1838-1 · Cited by 36 · see citing works
Independent highly sensitive characterization of asparagine deamidation and aspartic acid isomerization by sheathless CZE‐ESI‐MS/MS
Gahoual, R., Beck, A., François, Y., & Leize‐Wagner, E. (2016) · Journal of Mass Spectrometry · doi:10.1002/jms.3735 · Cited by 36 · see citing works
Capillary electrophoresis as a powerful tool for the analyses of bacterial samples
Kartsova, L. A., Makeeva, D. V., Kravchenko, A. V., Moskvichev, D. O., & Поликарпова, Д. А. (2020) · TrAC Trends in Analytical Chemistry · doi:10.1016/j.trac.2020.116110 · Cited by 35 · see citing works
Capillary zone electrophoresis tandem mass spectrometry detects low concentration host cell impurities in monoclonal antibodies
Zhu, G., Sun, L., Heidbrink‐Thompson, J., Kuntumalla, S., Lin, H., Larkin, C. J., McGivney, J. B., & Dovichi, N. J. (2016) · ELECTROPHORESIS · doi:10.1002/elps.201500301 · Cited by 34 · see citing works
A simple sheathless CE-MS interface with a sub-micrometer electrical contact fracture for sensitive analysis of peptide and protein samples
Nguyen, T. T. T. N., Petersen, N. J., & Rand, K. D. (2016) · Analytica Chimica Acta · doi:10.1016/j.aca.2016.07.002 · Cited by 33 · see citing works
Revealing the potential of capillary electrophoresis/mass spectrometry: the tipping point
Gahoual, R., Leize‐Wagner, E., Houzé, P., & François, Y. (2018) · Rapid Communications in Mass Spectrometry · doi:10.1002/rcm.8238 · Cited by 32 · see citing works
Inventory of proteoforms as a current challenge of proteomics: Some technical aspects.
Naryzhny, S. (2019) · Journal of Proteomics · doi:10.1016/j.jprot.2018.05.008 · Cited by 29 · see citing works
Capillary Zone Electrophoresis-Tandem Mass Spectrometry with Activated Ion Electron Transfer Dissociation for Large-scale Top-down Proteomics
McCool, E. N., Lodge, J. M., Basharat, A. R., Liu, X., Coon, J. J., & Sun, L. (2019) · Journal of the American Society for Mass Spectrometry · doi:10.1007/s13361-019-02206-6 · Cited by 28 · see citing works
Multidimensional Separations in Top-Down Proteomics
Guo, Y., Cupp-Sutton, K. A., Zhao, Z.-T., Anjum, S., & Wu, S. (2023) · Analytical Science Advances · doi:10.1002/ansa.202300016 · Cited by 25 · see citing works
Use of monolithic supports for high‐throughput protein and peptide separation in proteomics
Andjelković, U., Tufegdžić, S., & Popović, M. (2017) · Electrophoresis · doi:10.1002/elps.201700260 · Cited by 27 · see citing works
Pilot Evaluation of the Long-Term Reproducibility of Capillary Zone Electrophoresis–Tandem Mass Spectrometry for Top-Down Proteomics of a Complex Proteome Sample
Sadeghi, S. A., Chen, W., Wang, Q., Wang, Q., Fang, F., Liu, X., & Sun, L. (2024) · Journal of Proteome Research · doi:10.1021/acs.jproteome.3c00872 · Cited by 25 · see citing works
Total Biosynthesis of the Pyrrolo[4,2]benzodiazepine Scaffold Tomaymycin on an In Vitro Reconstituted NRPS System
von Tesmar, A., Hoffmann, M., Pippel, J., Fayad, A. G. A., Dausend-Werner, S., Bauer, A., Blankenfeldt, W., & Müller, R. (2017) · Cell chemical biology · doi:10.1016/j.chembiol.2017.08.001 · Cited by 25 · see citing works
Characterization of Post-Transcriptional RNA Modifications by Sheathless Capillary Electrophoresis–High Resolution Mass Spectrometry
Lechner, A., Wolff, P., Leize‐Wagner, E., & François, Y. (2020) · Analytical Chemistry · doi:10.1021/acs.analchem.0c01345 · Cited by 23 · see citing works
Capillary zone electrophoresis-tandem mass spectrometry with ultraviolet photodissociation (213 nm) for large-scale top–down proteomics
McCool, E. N., Chen, D., Li, W., Liu, Y., & Sun, L. (2019) · Analytical Methods · doi:10.1039/c9ay00585d · Cited by 22 · see citing works
Capillary Zone Electrophoresis-Electron-Capture Collision-Induced Dissociation on a Quadrupole Time-of-Flight Mass Spectrometer for Top-Down Characterization of Intact Proteins
Shen, X., Xu, T., Hakkila, B., Hare, M. G., Wang, Q., Wang, Q., Beckman, J. S., & Sun, L. (2021) · Journal of the American Society for Mass Spectrometry · doi:10.1021/jasms.0c00484 · Cited by 22 · see citing works
Capillary Electrophoresis–Mass Spectrometry for Top-Down Proteomics
Wang, Q., Wang, Q., Zhu, G., & Sun, L. (2025) · Annual Review of Analytical Chemistry · doi:10.1146/annurev-anchem-071124-092242 · Cited by 22 · see citing works
Top-down protein identification using isotopic envelope fingerprinting
Xiao, K., Yu, F., & Tian, Z. (2016) · Journal of Proteomics · doi:10.1016/j.jprot.2016.10.010 · Cited by 21 · see citing works
From Raw Data to Biological Discoveries: A Computational Analysis Pipeline for Mass Spectrometry-Based Proteomics
Lavallée‐Adam, M., Park, S. K. R., Martínez‐Bartolomé, S., He, L., & Yates, J. R. (2015) · Journal of the American Society for Mass Spectrometry · doi:10.1007/s13361-015-1161-7 · Cited by 20 · see citing works
Self-citation
Improvement of Mitochondria Extract fromSaccharomyces cerevisiaeCharacterization in Shotgun Proteomics Using Sheathless Capillary Electrophoresis Coupled to Tandem Mass Spectrometry
Ibrahim, M., Gahoual, R., Enkler, L., Becker, H. D., Chicher, J., Hammann, P., François, Y., Kühn, L., & Leize‐Wagner, E. (2016) · Journal of Chromatographic Science · doi:10.1093/chromsci/bmw005 · Cited by 16 · see citing works
Capillary Zone Electrophoresis as A Tool for Bottom-Up Protein Analysis
Wojcik, R., Zhu, G., Zhang, Z., Yan, X., Zhao, Y., Sun, L., Champion, M. M., & Dovic̀hi, N. J. (2015) · Bioanalysis · doi:10.4155/bio.15.227 · Cited by 16 · see citing works
CE-MS for Proteomics and Intact Protein Analysis
Kuzyk, V., Somsen, G. W., & Haselberg, R. (2021) · Advances in experimental medicine and biology · doi:10.1007/978-3-030-77252-9_4 · Cited by 15 · see citing works
Probing protein higher-order structures by native capillary electrophoresis-mass spectrometry
Zhang, W., Xiang, Y., & Xu, W. L. (2022) · TrAC Trends in Analytical Chemistry · doi:10.1016/j.trac.2022.116739 · Cited by 14 · see citing works
SpectroGene: A Tool for Proteogenomic Annotations Using Top-Down Spectra
Kolmogorov, M., Liu, X., & Pevzner, P. A. (2015) · Journal of Proteome Research · doi:10.1021/acs.jproteome.5b00610 · Cited by 14 · see citing works
A simple and efficient approach for preparing cationic coating with tunable electroosmotic flow for capillary zone electrophoresis-mass spectrometry-based top-down proteomics
Wang, Q., Gao, G., Fang, F., Wang, Q., Lundquist, P. K., & Sun, L. (2024) · Analytica Chimica Acta · doi:10.1016/j.aca.2024.343162 · Cited by 13 · see citing works
In-tip nanoreactors for cancer cells proteome profiling
Yan, L., Qiao, L., Ji, J., Li, Y., Yin, X., Lin, L., Liu, X., Yao, J., Wang, Y., Liu, B., et al. (2016) · Analytica Chimica Acta · doi:10.1016/j.aca.2016.11.021 · Cited by 11 · see citing works
Self-citation
Capillary Isoelectric Focusing of Proteins and Peptides Using an In-Line cIEF-ESI Interface with Improved MS Characteristics
Kerr, C. M., Schneider, O. L., Tichy, S. E., Huge, B. J., & Champion, M. M. (2025) · Analytical Chemistry · doi:10.1021/acs.analchem.4c05010 · Cited by 10 · see citing works
CE-MS for the analysis of intact proteins
Haselberg, R., & Somsen, G. W. (2016) · Wiley-VCH eBooks · doi:10.1002/9783527693801.ch7 · Cited by 10 · see citing works
Capillary zone electrophoresis-tandem mass spectrometry for top-down proteomics using attapulgite nanoparticles functionalized separation capillaries
Wang, T., Chen, D., Lubeckyj, R. A., Shen, X., Yang, Z., McCool, E. N., Qiao, X., & Sun, L. (2019) · Talanta · doi:10.1016/j.talanta.2019.04.040 · Cited by 10 · see citing works
Electrospray Regime Controls Sensitivity and Quantitative Performance in Micro- and Nano-Flow CE–ESI–MS
O'Neal, R. M., García, L. D., & Nemes, P. (2026) · Analytical Chemistry · doi:10.1021/acs.analchem.6c01323 · Cited by 7 · see citing works
Electrospray Ionization Interface Development for Capillary Electrophoresis-Mass Spectrometry
Risley, J. M., Jong, C. A. G. D., & Chen, D. D. Y. (2016) · Wiley-VCH eBooks · doi:10.1002/9783527693801.ch2 · Cited by 6 · see citing works
Proteoform-Specific Protein Binding of Small Molecules in Complex Matrices
Gil, G., Mao, P., Avula, B., Ali, Z., Chittiboyina, A. G., Khan, I. A., Walker, L., & Wang, D. (2016) · ACS Chemical Biology · doi:10.1021/acschembio.6b01018 · Cited by 5 · see citing works
Top-Down Proteomics by Capillary Zone Electrophoresis-Tandem Mass Spectrometry for Large-Scale Characterization of Proteoforms in Complex Samples
McCool, E. N., Lubeckyj, R. A., Chen, D., & Sun, L. (2022) · Methods in molecular biology · doi:10.1007/978-1-0716-2493-7_8 · Cited by 5 · see citing works
Capillary Electrophoresis-Mass Spectrometry for Proteomics
Martelli, C., & Desiderio, C. (2018) · Elsevier eBooks · doi:10.1016/b978-0-12-809375-7.00014-9 · Cited by 5 · see citing works
Sheathless CESI–MS versus LC–MS: Results of qualitative and quantitative analyses of the primary and secondary metabolites of Pleioblastus amarus bamboo shoots
Ren, Y., Liu, Y., Zhang, W., Ran, J., Li, L., & Zhang, Z. (2023) · Electrophoresis · doi:10.1002/elps.202300030 · Cited by 4 · see citing works
Reproducible Capillary Electrophoresis–Mass Spectrometry–Based Top‐Down Proteomics of Complex Proteomes Enabled by an Advanced Cationic Polymer Coating
Gao, G., Fang, F., Yue, Y., Zhang, A. T., Wang, Q., Wang, Q., Zhu, G., & Sun, L. (2025) · Journal of Mass Spectrometry · doi:10.1002/jms.70005 · Cited by 3 · see citing works
Advancing the Reproducibility and Repeatability of Capillary Zone Electrophoresis-Mass Spectrometry-Based Top-Down Proteomics by an Improved Capillary Coating Procedure
Yue, Y.-F., Fang, F., Gao, G., Sadeghi, S., Colón-Rosado, J. A., Nimavard, R. T., Askarani, M. F., Thorp, L., Dashtaki, M. R., Zhu, G., et al. (2026) · Journal of Proteome Research · doi:10.1021/acs.jproteome.5c01194 · Cited by 2 · see citing works
Magnetic Fe3O4@mTiO2-AIPA Microspheres for Separation of Phosphoproteins and Non-phosphoproteins
Tang, Q., Zhao, R., Lu, Q., & Qing, G. (2019) · Journal of Wuhan University of Technology-Mater Sci Ed · doi:10.1007/s11595-019-2113-z · Cited by 1 · see citing works
Proteoforms: General Concepts and Methodological Process for Identification
Araújo, J. D. S., & Machado, O. L. T. (2019) · Proteoforms - Concept and Applications in Medical Sciences · doi:10.5772/intechopen.89914 · Cited by 1 · see citing works
Characterization of Intact Therapeutics and Protein Subunits Using CE-MS Platform
Wang, W. (2016) · Springer eBooks · doi:10.1007/978-3-319-46240-0_7
Awkt: A Physicochemical Parameter Estimation Tool for Capillary Zone ElectrophoresisPreprint
Loeffeld, C. (2017) · arXiv (Cornell University) · doi:10.48550/arxiv.1706.03561
Proteomic and Metabolomic Analyses of Biological Systems with Liquid Chromatography Tandem Mass Spectrometry and Ion Mobility Mass Spectrometry
Maurer, M. M. (2017) · doi:10.33915/etd.6179
Capillary Zone Electrophoresis–Mass Spectrometry for Top‐Down Proteomics: Technological Development and Biological Applications
Sun, L. (2022) · Capillary Electrophoresis‐Mass Spectrometry for Proteomics and Metabolomics · doi:10.1002/9783527833092.ch10
Advances in Structural Proteomics using Mass Spectrometry
Otun, S., Mokhantso, T., & Achilonu, I. (2024) · Applications of modern mass spectrometry · doi:10.2174/9789815050059124020007
Characterization of therapeutic proteins by capillary electrophoresis (CE) coupled to mass spectrometry (MS)
Saïd, N. (2017) · doi:10.70675/a41cd9b1z5c4dz4d60z84fdz2821afe2e277
Apport de l'électrophorèse capillaire pour l'étude des anomalies de glycosylation de protéines liées à des pathologies : vers l'identification de nouveaux biomarqueurs
Ruel, C. (2018) · doi:10.70675/bbbf66daz24dfz4f61z8146z3e537087ba11
Développement de l'électrophorèse capillaire couplée à la spectrométrie de masse pour l'étude des modifications post-transcriptionnelles des ARN.
Lechner, A. (2020) · doi:10.70675/32f9b3fdz9716z4ae5zaf28zb85e431c27fe

Top-down proteomic analysis of protein pharmaceuticals, mixtures of protein complexes, and ribosomal protein extracts by capillary zone electrophoresis-mass spectrometry
Belov, A. M. (n.d.) · Digital Repository Service (Northeastern University)
In-Line Separation by Capillary Electrophoresis Prior to Analysis by Top-Down Mass Spectrometry Enables Sensitive Characterization of Protein Complexes
Han, X., Wang, Y., Aslanian, A., Fonslow, B., Graczyk, B., Davis, T. N., & Yates, J. R. (2014) · Journal of Proteome Research · doi:10.1021/pr500971h · Cited by 72 · see citing works
Self-citation
Optimizing capillary electrophoresis for top‐down proteomics of 30–80 kDa proteins
Li, Y., Compton, P. D., Tran, J. C., Ntai, I., & Kelleher, N. L. (2014) · PROTEOMICS · doi:10.1002/pmic.201300381 · Cited by 95 · see citing works
Methodologies and applications for the analysis of intact proteins and protein-ligand interactions by top-down mass spectrometry
Nshanian, M. (2018) · eScholarship (California Digital Library)
Top-Down Proteomics Using Sheath-Flow Capillary Electrophoresis Coupled to Mass Spectrometry
Zhao, Y. (2022) · Figshare · doi:10.7274/3r074t66h2p

Authors: Han, X., Wang, Y., Aslanian, A., Bern, M., Lavallée-Adam, M., & Yates, J. R.
Affiliations: Scripps Research Institute (US)
Palo Alto Research Center (US)
The Scripps Research Institute , , 10550 North Torrey Pines Road , , ,
Corresponding author: Yates, John R.
ORCID: Han, Xuemei
Wang, Yueju
Bern, Marshall
Yates, John R.
Venue: Analytical Chemistry
Publisher: American Chemical Society (ACS)
Published: 2014-10-26
Volume: 86
Issue: 22
Pages: 11006-11012
ISSN: 0003-2700 (ISSN-L)
1520-6882
DOI: 10.1021/ac503439n
PMC: PMC4238646
OpenAlex: W2323269825
Cited by: 77
References: 51
Citations by year: 2026: 2 · 2025: 3 · 2024: 3 · 2023: 4 · 2022: 3 · 2021: 5 · 2020: 6 · 2019: 9 · 2018: 5 · 2017: 10 · 2016: 16 · 2015: 11
FWCI: 5.28
Type: journal article
Language: en
Open access: hybrid
doi.org
Licence: other-oa
pubs.acs.org/page/policy/authorchoice_termsofuse.html (vor, 365 d)
Locations: Analytical Chemistry (publishedVersion, other-oa)
Europe PMC (PubMed Central) (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Funders: National Institutes of Health (8P41GM103533-17, R01 MH067880, R43 GM103352)
National Heart, Lung, and Blood Institute (HHSN268201000035C)
Topics: Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
Physical Sciences › Chemistry › Spectroscopy › Mass Spectrometry Techniques and Applications
Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Capillary Electrophoresis Applications
Keywords: top-down mass spectrometry, Pyrococcus furiosus, proteoforms, protein isoforms, post-translational modification, truncation, acetylation, disulfide bonds, oxidation, glycosylation, hypusine, cell lysate
MeSH terms: Electrophoresis, Capillary, Archaeal Proteins, Pyrococcus furiosus, Proteome, Proteomics, Tandem Mass Spectrometry

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Sheathless Capillary Electrophoresis-Tandem Mass Spectrometry for Top-Down Characterization of Pyrococcus furiosus Proteins on a Proteome Scale

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