Cited by 83Redox biology and oxidative stressAlcohol Consumption and Health EffectsAntioxidant Activity and Oxidative Stress

Increased oxidation and degradation of cytosolic proteins in alcohol-exposed mouse liver and hepatoma cells

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Kim, Bong‐Jo, Hood, Brian L., Aragon, Richard A., Hardwick, James P., Conrads, Thomas P., Veenstra, Timothy D., Song, Byoung J.·2006·PROTEOMICS

Abstract

We recently developed a sensitive method using biotin-N-maleimide (biotin-NM) as a probe to positively identify oxidized mitochondrial proteins. In this study, biotin-NM was used to identify oxidized cytosolic proteins in alcohol-fed mouse livers. Alcohol treatment for 6 wk elevated the levels of CYP2E1 and nitrotyrosine, a marker of oxidative stress. Markedly increased levels of oxidized proteins were detected in alcohol-fed mouse livers compared to pair-fed controls. The biotin-NM-labeled oxidized proteins from alcohol-exposed mouse livers were subsequently purified with streptavidin-agarose and resolved on 2-DE. More than 90 silver-stained protein spots that displayed differential intensities on 2-D gels were identified by MS. Peptide sequence analysis revealed that many enzymes or proteins involved in stress response, chaperone activity, intermediary metabolism, and antioxidant defense systems such as peroxiredoxin were oxidized after alcohol treatment. Smaller fragments of many proteins were repeatedly detected only in alcohol-fed mice, indicating that many oxidized proteins after alcohol exposure were degraded. Immunoblot results showed that the level of oxidized peroxiredoxin (inactivated) was markedly increased in the alcohol-exposed mouse livers and ethanol-sensitive hepatoma cells compared to the corresponding controls. Our results may explain the underlying mechanism for cellular dysfunction and increased susceptibility to other toxic agents following alcohol-mediated oxidative stress.

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  1. [7]Wu D, Cederbaum AI. Alcohol Clin Exp Res. 1999;23:67–76. [PubMed] [Google Scholar]
  2. [10]Takahashi T, Lasker JM, Rosman AS, Lieber CS. Hepatology. 1993;17:236–245. [PubMed] [Google Scholar]
  3. [11]Miles MF, Wilke N, Elliot M, Tanner W, Shah S. Mol Pharmacol. 1994;46:873–879. [PubMed] [Google Scholar]
  4. [14]Kono H, Rusyn I, Bradford BU, Connor HD, et al. J Pharmacol Exp Ther. 2000;293:296–303. [PubMed] [Google Scholar]
  5. [25]Bae MA, Pie JE, Song BJ. Mol Pharmacol. 2001;60:847–856. [PubMed] [Google Scholar]
  6. [32]Lin Z, Crockett DK, Lim MS, Elenitoba-Johnson KS. J Biomol Tech. 2003;14:149–155. [PMC free article] [PubMed] [Google Scholar]
  7. [43]Rivett AJ. J Biol Chem. 1985;260:300–305. [PubMed] [Google Scholar]
  8. [44]Davies KJA. J Biol Chem. 1987;262:9895–9901. [PubMed] [Google Scholar]
  9. [50]Grune T, Reinheckel T, Davies KJA. FASEB J. 1997;11:526–534. [PubMed] [Google Scholar]
  10. [54]Song BJ, Veech RL, Park SS, Gelboin HV, Gonzalez FJ. J Biol Chem. 1989;264:3568–3572. [PubMed] [Google Scholar]
  11. [57]Israel Y. Gastroenterology. 1997;113:353–355. [PubMed] [Google Scholar]

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Authors
Kim, B., Hood, B. L., Aragon, R. A., Hardwick, J. P., Conrads, T. P., Veenstra, T. D., & Song, B. J.
Affiliations
  • National Institute on Alcohol Abuse and Alcoholism (US)
  • Science Applications International Corporation (United States) (US)
  • Northeast Ohio Medical University (US)
Corresponding author
Song, Byoung J.
Venue
PROTEOMICS
Publisher
Wiley
Published
2006-02
Volume
6
Issue
4
Pages
1250-1260
ISSN
  • 1615-9853 (ISSN-L)
  • 1615-9861
OpenAlex
W2159676392
Cited by
83
References
65
Citations by year
2025: 1 · 2022: 1 · 2021: 3 · 2020: 2 · 2019: 2 · 2018: 4 · 2017: 1 · 2016: 1 · 2015: 4 · 2014: 5 · 2013: 5 · 2012: 6
FWCI
4.35
Type
journal article
Language
en
Open access
Locations
  • PROTEOMICS (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
  • DigitalCommons-Cedarville (Cedarville University) (submittedVersion)
Funders
  • National Institutes of Health (n01co12400-79-0-22)
  • National Institute on Alcohol Abuse and Alcoholism (Z01AA000036)
  • National Cancer Institute (N01CO12400, N01-CO-012400)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
  • Health Sciences › Medicine › Pathology and Forensic Medicine › Alcohol Consumption and Health Effects
  • Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
Keywords
alcohol-induced oxidative stress, protein oxidation, protein degradation, cytosolic proteins, peroxiredoxin, CYP2E1, nitrotyrosine, liver, antioxidant defense, molecular chaperones, stress response
MeSH terms
Ethanol, Animals, Bacterial Proteins, Biotin, Central Nervous System Depressants, Cytosol, Carcinoma, Hepatocellular, Liver, Liver Extracts, Liver Neoplasms, Male, Mice, Inbred C57BL, Oxidation-Reduction, Peroxidases, Sepharose, Biomarkers, Tumor, Tyrosine, Electrophoresis, Gel, Two-Dimensional, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Computational Biology, Cytochrome P-450 CYP2E1, Mice, Peroxiredoxins

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