Fucoidan increases TNF-α-induced MMP-9 secretion in monocytic cell line U937
Abstract
Objective To investigate the effect of fucoidan on the expression of matrix metalloproteinase-9 (MMP-9) from monocytes.Methods Human monocytic cell line U937 was purchased from ATCC. During the experiment, FBS-free 1640 was used and U937 was cultivated with 20 ng/ml TNF-alpha and/or different concentrations of fucoidan for 24 h. RT-PCR experiments were used to determine the MMP-9 mRNA expression. ELISA and gelatin zymography detected MMP-9 amounts and activity in the supernatant. The intracellular level of MMP-9 was assayed by Western blot, and the level of CD44 on the surface was assayed by FACS.Results In this study, we showed that pro-inflammatory cytokine TNF-alpha up-regulated U937 MMP-9 mRNA and protein levels (P < 0.05). Fucoidan can increase the TNF-alpha-induced MMP-9 secretion from U937 (P < 0.05), but no significant difference was observed in MMP-9 mRNA. The intracellular level of MMP-9 treated with TNF-alpha and fucoidan was lower (P < 0.05) than that treated with TNF-alpha alone. In addition, we demonstrated that fucoidan downregulated the surface level of CD44, the main molecule to which MMP-9 attaches.Conclusions We demonstrated that fucoidan post-translationally regulated MMP-9 secretion from U937. Reduced intracellular level and decreased membrane attachment may contribute to the increase in MMP-9 secretion.
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Fucoidan from F. vesiculosus post-translationally regulated MMP-9 secretion from human monocyte cell line U937.
“The fucoidan from F. vesiculosus post-translationally regulated MMP-9 secretion from human monocyte cell line U937 [71].”
From Anticancer and Cancer Preventive Properties of Marine Polysaccharides: Some Results and Prospects (2013) · cites this paper as [71]
Cites
Strategies for MMP inhibition in cancer: innovations for the post-trial era
Overall, C. M., & López-Otín, C. (2002) · Nature Reviews Cancer · doi:10.1038/nrc884 · Cited by 1,349 · see citing works
Gelatinase B: a tuner and amplifier of immune functions
Opdenakker, G., den Steen, P. E. V., & Damme, J. V. (2001) · Trends in Immunology · doi:10.1016/s1471-4906(01)02023-3 · Cited by 398 · see citing works
A study of fucoidan from the brown seaweed Chorda filum
Chizhov, A. O., Dell, A., Morris, H. R., Haslam, S. M., McDowell, R. A., Shashkov, A. S., Nifant’ev, N. E., Khatuntseva, E. A., & Usov, A. I. (1999) · Carbohydrate Research · doi:10.1016/s0008-6215(99)00148-2 · Cited by 326 · see citing works
Moon, S., Cha, B., & Kim, C. (2003) · Journal of Cellular Physiology · doi:10.1002/jcp.10435 · Cited by 299 · see citing works
(2003) · PubMed · Cited by 170 · see citing works
Immunomodulating Activity of Arabinogalactan and Fucoidan In Vitro
Choi, E., Kim, A.-J., Kim, Y.-O., & Hwang, J. (2005) · Journal of Medicinal Food · doi:10.1089/jmf.2005.8.446 · Cited by 158 · see citing works
Cell surface association of matrix metalloproteinase-9 (gelatinase B)
Fridman, R. A., Tóth, M., Chvyrkova, I., Meroueh, S. O., & Mobashery, S. (2003) · Cancer and Metastasis Reviews · doi:10.1023/a:1023091214123 · Cited by 155 · see citing works
Fucoidan inhibits UVB-induced MMP-1 expression in human skin fibroblasts
Moon, H. J., Lee, S. R., Shim, S. N., Jeong, S. H., Stonik, V. A., Rasskazov, V. A., Zvyagintseva, T., & Lee, Y. H. (2008) · Biological and Pharmaceutical Bulletin · doi:10.1248/bpb.31.284 · Cited by 131 · see citing works
Ma, Z., Shah, R. C., Chang, M. J., & Benveniste, E. N. (2004) · Molecular and Cellular Biology · doi:10.1128/mcb.24.12.5496-5509.2004 · Cited by 117 · see citing works
Effects of PDGF-C and PDGF-D on monocyte migration and MMP-2 and MMP-9 expression
Wågsäter, D., Zhu, C., Björck, H. M., & Eriksson, P. O. (2008) · Atherosclerosis · doi:10.1016/j.atherosclerosis.2008.04.050 · Cited by 88 · see citing works
Zen, K., Liu, Y., Cairo, D., & Parkos, C. A. (2002) · The Journal of Immunology · doi:10.4049/jimmunol.169.9.5270 · Cited by 87 · see citing works
Fucoidan stimulation induces a functional maturation of human monocyte-derived dendritic cells
Yang, M., Ma, C., Sun, J., Shao, Q., Gao, W., Zhang, Y., Li, Z., Xie, Q., Dong, Z., & Qu, X. (2008) · International Immunopharmacology · doi:10.1016/j.intimp.2008.08.007 · Cited by 78 · see citing works
Fucoidan prevents Cε germline transcription and NFκB p52 translocation for IgE production in B cells
Oomizu, S., Yanase, Y., Suzuki, H., Kameyoshi, Y., & Hide, M. (2006) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2006.08.009 · Cited by 71 · see citing works
Rahat, M. A., Marom, B., Bitterman, H., et al. (2006) · Journal of Leukocyte Biology · doi:10.1189/jlb.0605302 · Cited by 67 · see citing works
Ho, T., Yan, W., & Bagnell, C. A. (2007) · Journal of Leukocyte Biology · doi:10.1189/jlb.0906556 · Cited by 35 · see citing works
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Wijesinghe, W. A. J. P., & Jeon, Y.-J. (2012) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2011.12.029 · Cited by 449 · see citing works
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Tanna, B., & Mishra, A. (2019) · Comprehensive Reviews in Food Science and Food Safety · doi:10.1111/1541-4337.12441 · Cited by 329 · see citing works
Anticancer and Cancer Preventive Properties of Marine Polysaccharides: Some Results and Prospects
Fedorov, S., Ermakova, S., Zvyagintseva, T., & Stonik, V. (2013) · Marine Drugs · doi:10.3390/md11124876 · Cited by 189 · see citing works
- Authors
- Jintang, S., Alei, F., Yun, Z., Shanzhen, S., Weixu, H., Meixiang, Y., Fengcai, W., & Xun, Q.
- Affiliations
- Shandong University (CN)
- Venue
- Inflammation Research
- Published
- 2009-09-23
- Volume
- 59
- Issue
- 4
- Pages
- 271-276
- ISSN
- 1023-3830 (ISSN-L)
- 1420-908X
- OpenAlex
- W1994623714
- Cited by
- 27
- References
- 15
- Citations by year
- 2025: 1 · 2023: 2 · 2022: 1 · 2021: 1 · 2020: 3 · 2019: 1 · 2018: 1 · 2017: 3 · 2016: 3 · 2013: 4 · 2012: 1
- FWCI
- 3.52
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Inflammation Research (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China
- Natural Science Foundation of Shandong Province
- Topics
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Protease and Inhibitor Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Proteoglycans and glycosaminoglycans research
- Keywords
- fucoidan, MMP-9, TNF-α, U937 cells, monocytes, MMP-9 secretion, CD44, post-translational regulation, inflammatory cytokines
- MeSH terms
- Antibodies, Monoclonal, Antineoplastic Agents, Culture Media, Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Humans, Indicators and Reagents, Monocytes, Polysaccharides, RNA, Transcription, Genetic, Tumor Necrosis Factor-alpha, Blotting, Western, Down-Regulation, Hyaluronan Receptors, Reverse Transcriptase Polymerase Chain Reaction, U937 Cells, Matrix Metalloproteinase 9
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