Anti-proliferative activity of oversulfated fucoidan from commercially cultured Cladosiphon okamuranus TOKIDA in U937 cells
Abstract
Fucoidan from Cladosiphon okamuranus and its sulfate derivatives were prepared. Sulfate contents of native and oversulfated fucoidan were estimated to be 13.5% and 32.8%, respectively. The results of (1)H NMR suggest that 2,4-di-O-sulfo-, 2-mono-O-sulfo- and 4-mono-O-sulfo-l-fucopyranose were involved in oversulfated fucoidan and 4-mono-O-sulfo-l-fucopyranose was involved in native fucoidan. The oversulfated fucoidan reduced the proliferation of U937 cells in a dose-dependent manner, but the activity of native fucoidan was weak. The sulfate content and substituting position of sulfate group might be important factors of anti-proliferative activity in U937 cells. To examine whether the anti-proliferative activity of oversulfated fucoidan was caused by induction of apoptosis, apoptosis assay, caspase-3 activity assay and Western blotting analysis were performed. These results indicated that the oversulfated fucoidan induced apoptosis via caspase-3 and -7 activation-dependent pathway.
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Native and oversulfated FCSPs from Cladosiphon okamuranus: natural sulfation produced 4-mono-O-sulfo-l-fucopyranose whereas oversulfated FCSPs contained 2,4-di-, 2-mono-, and 4-mono-O-sulfo-l-fucopyranose.
“Native and oversulfated FCSPs derived from Cladosiphon okamuranus (Chordariales) were analyzed using 1H NMR spectroscopy and it was suggested that whereas natural sulfation produced 4-mono-O-sulfo-l-fucopyranose the oversulfated FCSPs contained 2,4-di-, 2-mono-, and 4-mono-O-sulfo-l-fucopyranose [89].”
Sulfate content and positioning of sulfate groups (e.g., 2,4-di- vs. 4-mono) might be important for anti-proliferative activity of fucoidan in human leukemia cell line U937, presumed via induction of apoptosis associated with activation of caspase-3 and -7.
“It was also suggested that sulfate content and the positioning of sulfate groups, e.g., 2,4-di- vs. 4-mono, might be important for the anti-proliferative activity of fucoidan in a human leukemia cell line (U937), an effect which is presumed to take place via induction of apoptosis associated with activation of caspase-3 and -7 [89].”
Oversulfated fucoidan induced apoptosis through caspase-3 and -7 activation.
“These results indicated that oversulfated fucoidan induced apoptosis through caspase-3 and -7 activation.”
From Anticancer and Cancer Preventive Properties of Marine Polysaccharides: Some Results and Prospects (2013) · cites this paper as [52]
Antitumor activity of fucoidan may be a result of an indirect effect associated with enhancement of cytolytic activity of NK cells augmented by increased production of macrophage-mediated immune response signaling molecules IL-2, IFN-γ and IL-12.
“Some findings indicate that the antitumor activity of fucoidan is a result of an indirect effect which may be associated with a significant enhancement of the cytolytic activity of natural killer (NK) cells augmented by increased production of macrophage-mediated immune response signaling molecules, 50,51 namely interleukins (IL)-2, IFN-c and IL-12.50”
From Fucoidans from brown seaweeds: an update on structures, extraction techniques and use of enzymes as tools for structural elucidation (2013) · cites this paper as [51]
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- Authors
- Teruya, T., Konishi, T., Uechi, S., Tamaki, H., & Tako, M.
- Affiliations
- Kagoshima University (JP)
- University of the Ryukyus (JP)
- Corresponding author
- Tako, Masakuni
- Venue
- International Journal of Biological Macromolecules
- Publisher
- Elsevier BV
- Published
- 2007-08
- Volume
- 41
- Issue
- 3
- Pages
- 221-226
- ISSN
- 0141-8130 (ISSN-L)
- OpenAlex
- W2002472283
- Cited by
- 156
- References
- 25
- Citations by year
- 2026: 3 · 2025: 4 · 2024: 5 · 2023: 7 · 2022: 11 · 2021: 12 · 2020: 9 · 2019: 4 · 2018: 5 · 2017: 8 · 2016: 10 · 2015: 6 · 2014: 11 · 2013: 15 · 2012: 5
- FWCI
- 12.42
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- International Journal of Biological Macromolecules (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
- Physical Sciences › Earth and Planetary Sciences › Oceanography › Marine and coastal plant biology
- Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
- Keywords
- Cladosiphon okamuranus, antiproliferative activity, U937 cells, apoptosis, caspase-3, caspase-7, sulfated polysaccharides, seaweed polysaccharides
- MeSH terms
- Phaeophyceae, Antineoplastic Agents, Dose-Response Relationship, Drug, Humans, Polysaccharides, Apoptosis, U937 Cells, Cell Proliferation, Caspase 3, Caspase 7
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